摘要
在双叉犀金龟人工饲养过程中,连续2年出现了的拟寄生现象,对拟寄生的寄主种类进行鉴定。形态鉴定:寄主的幼虫、蛹和成虫均符合蛆症异蚤蝇(Megaselia scalaris)的形态特征;DNA条形码鉴定:扩增的842 bp碱基含量分别为A:29.6%,T:37.9%,G:16.2%,C:16.3%,A+T:67.5%,DNA碱基序列与BOLD(The Barcode of Life Data system)中收录蛆症异蚤蝇的序列相似度为100%,BOLD库中结果有20个序列推荐支持。综合形态和DNA条形码的鉴定结果,确定拟寄生种类为蛆症异蚤蝇(Megaselia scalaris)。双叉犀金龟拟寄生蚤蝇的鉴定为鞘翅目的生物防治提供了新证据。
During the artificial breeding process of Allomyrina dichotoma,there has been a clear phenomenon of parasitism for two consecutive years.The host species of parasitism were now identified.Morphological identification:The larvae,pupae,and adults of the host all conformed to the morphological characteristics of Megaselia scalaris.DNA barcode identification:The amplified 842 bp base contents were A:29.6%,T:37.9%,G:16.2%,C:16.3%,A+T:67.5%.The DNA based sequence has a 100%confidence similarity to the sequence of Megaselia scalaris included in BOLD(The Barcode of Life Data System).There were 20 sequence recommendations supported in the BOLD library.Based on the identification results of morphology and DNA barcode,it was determined that the parasitic species is Megaselia scalaris.The identification of Megaselia scalaris as a parasitic flea fly provided a new evidence for biological control of the Coleoptera.
作者
田子禾
张卓
李雯静
张艺琼
李思颐
王金霞
TIAN Zihe;ZHANG Zhuo;LI Wenjing;ZHANG Yiqiong;LI Siyi;WANG Jinxia(College of Life and Bioengineering,Shenyang University,Shenyang 110044,China;Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security,Shenyang University,Shenyang 110044,China)
出处
《佳木斯大学学报(自然科学版)》
2025年第11期165-167,53,共4页
Journal of Jiamusi University:Natural Science Edition
基金
国家自然基金面上项目(32071860)
2024年国家级大学生创新创业项目(202411035018)。
关键词
双叉犀金龟
蛆症异蚤蝇
形态鉴定
DNA条形码
Allomyrina dichotoma
Megaselia scalaris
Morphological identification
DNA barcoding
