促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)级联是植物中重要的信号传递通路,参与调控植株的多种生物和非生物胁迫。为研究SlMAPK3对番茄耐旱方面的影响,本试验以野生型番茄M82及其过表达SlMAPK3株系OE1、OE3、OE4...促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)级联是植物中重要的信号传递通路,参与调控植株的多种生物和非生物胁迫。为研究SlMAPK3对番茄耐旱方面的影响,本试验以野生型番茄M82及其过表达SlMAPK3株系OE1、OE3、OE4为研究对象,定植田间后,6~7片真叶期进行中度干旱处理保持田间持水量45%~55%,于开花期,统计植株的高度、结果量,比较了不同株系之间果实大小、果实产量以及植株叶片抗氧化活性差异。在田间条件研究中发现,SlMAPK3过表达番茄株系的耐性更强,暗示SlMAPK3在番茄抗旱中发挥重要调控作用,为番茄抗逆育种奠定基础。展开更多
Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances...Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances.Therefore,we obtained 2 peptides from salted egg white:Val-Val-His-Phe(VF-4)and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg(DR-8).The sodium dextran sulfate(DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo.The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis,attenuated colon tissue damage,inhibited the activation of nuclear factor kappa-B(NF-κB)/mitogen-activated protein kinase(MAPK)/phosphoinositide 3-kinase-Akt(PI3K-AKT)signaling pathways,and inhibited the expression of inflammatory cytokines.16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus,Blautia,and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter,Lachnospiraceae_NK4A136_group,Klebsiella.Moreover,the degree of correlation between pro-inflammatory cytokines and microbiota was as follows:interleukin-6(IL-6)>tumor necrosis factor-α(TNF-α)>interleukin-1β(IL-1β)>interferon-γ(IFN-γ).In conclusion,this study suggests that salted egg white peptides VF-4 and DR-8 have a significant antiinflammatory effect in vivo.It also provides a strategy for the treatment of IBD and a new way for the highvalue utilization of salted egg white.展开更多
文摘促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)级联是植物中重要的信号传递通路,参与调控植株的多种生物和非生物胁迫。为研究SlMAPK3对番茄耐旱方面的影响,本试验以野生型番茄M82及其过表达SlMAPK3株系OE1、OE3、OE4为研究对象,定植田间后,6~7片真叶期进行中度干旱处理保持田间持水量45%~55%,于开花期,统计植株的高度、结果量,比较了不同株系之间果实大小、果实产量以及植株叶片抗氧化活性差异。在田间条件研究中发现,SlMAPK3过表达番茄株系的耐性更强,暗示SlMAPK3在番茄抗旱中发挥重要调控作用,为番茄抗逆育种奠定基础。
基金the financial support provided by the Project of Jiangxi Provincial Department of Education,China(GJJ200433)。
文摘Inflammation underlies many chronic diseases,and inflammatory bowel disease(IBD)is a condition characterized by long-term inflammation of the gut.Egg whites have been shown to contain many beneficial active substances.Therefore,we obtained 2 peptides from salted egg white:Val-Val-His-Phe(VF-4)and Asp-Thr-Gln-Ala-Met-Pro-Phe-Arg(DR-8).The sodium dextran sulfate(DSS)-induced mice colitis model was used to evaluate its regulatory effect on colitis in vivo.The results showed that VF-4 and DR-8 improved the clinical symptoms of DSS-induced colitis,attenuated colon tissue damage,inhibited the activation of nuclear factor kappa-B(NF-κB)/mitogen-activated protein kinase(MAPK)/phosphoinositide 3-kinase-Akt(PI3K-AKT)signaling pathways,and inhibited the expression of inflammatory cytokines.16S rRNA gene sequencing showed that VF-4 and DR-8 administration increased the relative abundance of intestinal beneficial bacteria including Lactobacillus,Blautia,and down-regulated the relative abundance of inflammation-related bacteria including Acinetobacter,Lachnospiraceae_NK4A136_group,Klebsiella.Moreover,the degree of correlation between pro-inflammatory cytokines and microbiota was as follows:interleukin-6(IL-6)>tumor necrosis factor-α(TNF-α)>interleukin-1β(IL-1β)>interferon-γ(IFN-γ).In conclusion,this study suggests that salted egg white peptides VF-4 and DR-8 have a significant antiinflammatory effect in vivo.It also provides a strategy for the treatment of IBD and a new way for the highvalue utilization of salted egg white.
