Momordica antiviral protein 30 kD(MAP30)is a type I ribosome-inactivating protein(RIP)with antibacterial,anti-HIV and antitumor activities but lacks the ability to target tumor cells.To increase its tumor-targeting ab...Momordica antiviral protein 30 kD(MAP30)is a type I ribosome-inactivating protein(RIP)with antibacterial,anti-HIV and antitumor activities but lacks the ability to target tumor cells.To increase its tumor-targeting ability,the arginine-glycine-aspartic(RGD)peptide and the epidermal growth factor receptor interference(EGFRi)peptide were fused with MAP30,which was named ELRL-MAP30.The efficiency of targeted therapy for triple-negative breast cancer(TNBC)MDA-MB-231 cells,which lack the expression of estrogen receptor(ER),Progesterone receptor(PgR)and human epidermal growth factor receptor-2(HER2),is limited.In this study,we focus on exploring the effect and mechanism of ELRL-MAP30 on TNBC MDA-MB-231 cells.First,we discovered that ELRL-MAP30 significantly inhibited the migration and invasion of MDA-MB-231 cells and induced MDA-MB-231 cell apoptosis.Moreover,ELRL-MAP30 treatment resulted in a significant increase in Bax expression and a decrease in Bcl-2 expression.Furthermore,ELRL-MAP30 triggered apoptosis via the Fak/EGFR/Erk and Ilk/Akt signaling pathways.In addition,recombinant ELRL-MAP30 can inhibit chicken embryonic angiogenesis,and also inhibit the tube formation ability of human umbilical vein endothelial cells(HUVECs),indicating its potential therapeutic effects on tumor angiogenesis.Collectively,these results indicate that ELRL-MAP30 has significant tumor-targeting properties in MDA-MB-231 cancer cells and reveals potential therapeutic effects on angiogenesis.These findings indicate the potential role of ELRL-MAP30 in the targeted treatment of the TNBC cell line MDA-MB-231.展开更多
苦瓜是药食同源的蔬菜,其果实中含有多种有效成分,具有降血糖、抗病毒、抗肿瘤、抗生育、抗氧化、抑菌等功能特性。文章综述了近年来国内外苦瓜及苦瓜蛋白MAP30(Momordica anti-HIV Protein of 30 ku)的研究情况,阐述了苦瓜的保健功能...苦瓜是药食同源的蔬菜,其果实中含有多种有效成分,具有降血糖、抗病毒、抗肿瘤、抗生育、抗氧化、抑菌等功能特性。文章综述了近年来国内外苦瓜及苦瓜蛋白MAP30(Momordica anti-HIV Protein of 30 ku)的研究情况,阐述了苦瓜的保健功能及其在医学领域的应用研究进展,并展望了其研究应用前景。展开更多
目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检...目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检测细胞培养上清液中细胞色素c(Cytc)和肿瘤坏死因子-α(TNF-α)的水平;免疫细胞化学法检测细胞ERK1/PERK1,AKT/PAKT和NF-κB表达;荧光微板阅读仪检测细胞caspase-12活性。结果 MAP30诱导可以使EC-1.71细胞固缩,核膜扭曲,核染色质聚集成块并靠近核膜,内质网高度膨胀、扩张;ΔΨm水平下降;Cytc和TNF-α含量增加;胞质中ERK1、AKT表达增强而胞核中PERK1、PAKT表达降低,NF-κB活性降低,caspase-12活性增强。结论 MAP30可以诱导EC-1.71细胞凋亡。展开更多
文摘Momordica antiviral protein 30 kD(MAP30)is a type I ribosome-inactivating protein(RIP)with antibacterial,anti-HIV and antitumor activities but lacks the ability to target tumor cells.To increase its tumor-targeting ability,the arginine-glycine-aspartic(RGD)peptide and the epidermal growth factor receptor interference(EGFRi)peptide were fused with MAP30,which was named ELRL-MAP30.The efficiency of targeted therapy for triple-negative breast cancer(TNBC)MDA-MB-231 cells,which lack the expression of estrogen receptor(ER),Progesterone receptor(PgR)and human epidermal growth factor receptor-2(HER2),is limited.In this study,we focus on exploring the effect and mechanism of ELRL-MAP30 on TNBC MDA-MB-231 cells.First,we discovered that ELRL-MAP30 significantly inhibited the migration and invasion of MDA-MB-231 cells and induced MDA-MB-231 cell apoptosis.Moreover,ELRL-MAP30 treatment resulted in a significant increase in Bax expression and a decrease in Bcl-2 expression.Furthermore,ELRL-MAP30 triggered apoptosis via the Fak/EGFR/Erk and Ilk/Akt signaling pathways.In addition,recombinant ELRL-MAP30 can inhibit chicken embryonic angiogenesis,and also inhibit the tube formation ability of human umbilical vein endothelial cells(HUVECs),indicating its potential therapeutic effects on tumor angiogenesis.Collectively,these results indicate that ELRL-MAP30 has significant tumor-targeting properties in MDA-MB-231 cancer cells and reveals potential therapeutic effects on angiogenesis.These findings indicate the potential role of ELRL-MAP30 in the targeted treatment of the TNBC cell line MDA-MB-231.
文摘苦瓜是药食同源的蔬菜,其果实中含有多种有效成分,具有降血糖、抗病毒、抗肿瘤、抗生育、抗氧化、抑菌等功能特性。文章综述了近年来国内外苦瓜及苦瓜蛋白MAP30(Momordica anti-HIV Protein of 30 ku)的研究情况,阐述了苦瓜的保健功能及其在医学领域的应用研究进展,并展望了其研究应用前景。
文摘目的探讨用重组苦瓜蛋白MAP30(Momordica anti-HIV protein of 30 ku)诱导人食管癌细胞株EC-1.71细胞凋亡状况。方法 MAP30诱导EC-1.71细胞后,透射电镜观察细胞凋亡的形态特征;JC-1荧光探针检测细胞线粒体膜电位(ΔΨm)变化;ELISA法检测细胞培养上清液中细胞色素c(Cytc)和肿瘤坏死因子-α(TNF-α)的水平;免疫细胞化学法检测细胞ERK1/PERK1,AKT/PAKT和NF-κB表达;荧光微板阅读仪检测细胞caspase-12活性。结果 MAP30诱导可以使EC-1.71细胞固缩,核膜扭曲,核染色质聚集成块并靠近核膜,内质网高度膨胀、扩张;ΔΨm水平下降;Cytc和TNF-α含量增加;胞质中ERK1、AKT表达增强而胞核中PERK1、PAKT表达降低,NF-κB活性降低,caspase-12活性增强。结论 MAP30可以诱导EC-1.71细胞凋亡。
