目的:探讨利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术快速鉴定灵芝的可行性。方法:采集灵芝菌丝、子实体、孢子样本后,用Autof ms800全自动微生物质谱检测系统检测,Autof Acquirer进行分析处理。结果:经过检测,得到了灵...目的:探讨利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术快速鉴定灵芝的可行性。方法:采集灵芝菌丝、子实体、孢子样本后,用Autof ms800全自动微生物质谱检测系统检测,Autof Acquirer进行分析处理。结果:经过检测,得到了灵芝的蛋白指纹图谱。其蛋白指纹图谱可以分为四部分:菌丝图谱、产孢子前子实体图谱、产孢子后子实体图谱、孢子图谱。以此初步建立数据库后,可快速鉴定未知样本是否为本品种灵芝。结论:MALDI-TOF-MS技术快速鉴定灵芝具有可行性。Objective: To explore the feasibility of rapid identification of Ganoderma lucidum by using MALDI-TOF-MS technique. Methods: The samples of Ganoderma lucidum mycelium, fruiting bodies, and spores were collected first and then detected by Autof ms800 automatic microbial mass spectrometry system and analyzed by Autof Acquirer finally. Results: After testing, the protein fingerprint of Ganoderma lucidum was obtained. Its protein fingerprint can be divided into four parts: hyphal profile, pre-sporulation fruiting body profile, post-sporulation fruiting body profile, and spore profile. After initially establishing a database with this, it is possible to quickly identify whether unknown samples are of this variety of Ganoderma lucidum. Conclusion: It is feasible for rapid identification of Ganoderma lucidum by MALDI-TOF-MS technique.展开更多
目的通过扩充基质辅助激光解吸电离飞行时间质谱(matrixassisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF-MS)的数据库研究宋内志贺菌的鉴定。方法用提取法进行宋内志贺菌质谱图的建库,建库后分别使...目的通过扩充基质辅助激光解吸电离飞行时间质谱(matrixassisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF-MS)的数据库研究宋内志贺菌的鉴定。方法用提取法进行宋内志贺菌质谱图的建库,建库后分别使用直接涂抹法和提取法对50株宋内志贺菌野生株进行鉴定。结果提取法的鉴定符合率为100%,而直接涂抹法的鉴定符合率为60%。结论 MALDI-TOF-MS通过数据库的扩充和流程的优化可以鉴定宋内志贺菌到血清学水平,它将会成为临床微生物实验室的诊断和鉴别工具。展开更多
文摘目的:探讨利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术快速鉴定灵芝的可行性。方法:采集灵芝菌丝、子实体、孢子样本后,用Autof ms800全自动微生物质谱检测系统检测,Autof Acquirer进行分析处理。结果:经过检测,得到了灵芝的蛋白指纹图谱。其蛋白指纹图谱可以分为四部分:菌丝图谱、产孢子前子实体图谱、产孢子后子实体图谱、孢子图谱。以此初步建立数据库后,可快速鉴定未知样本是否为本品种灵芝。结论:MALDI-TOF-MS技术快速鉴定灵芝具有可行性。Objective: To explore the feasibility of rapid identification of Ganoderma lucidum by using MALDI-TOF-MS technique. Methods: The samples of Ganoderma lucidum mycelium, fruiting bodies, and spores were collected first and then detected by Autof ms800 automatic microbial mass spectrometry system and analyzed by Autof Acquirer finally. Results: After testing, the protein fingerprint of Ganoderma lucidum was obtained. Its protein fingerprint can be divided into four parts: hyphal profile, pre-sporulation fruiting body profile, post-sporulation fruiting body profile, and spore profile. After initially establishing a database with this, it is possible to quickly identify whether unknown samples are of this variety of Ganoderma lucidum. Conclusion: It is feasible for rapid identification of Ganoderma lucidum by MALDI-TOF-MS technique.
文摘目的通过扩充基质辅助激光解吸电离飞行时间质谱(matrixassisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF-MS)的数据库研究宋内志贺菌的鉴定。方法用提取法进行宋内志贺菌质谱图的建库,建库后分别使用直接涂抹法和提取法对50株宋内志贺菌野生株进行鉴定。结果提取法的鉴定符合率为100%,而直接涂抹法的鉴定符合率为60%。结论 MALDI-TOF-MS通过数据库的扩充和流程的优化可以鉴定宋内志贺菌到血清学水平,它将会成为临床微生物实验室的诊断和鉴别工具。
