目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5...目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5%无水乙醇)和低、中、高浓度枳葛保肝降脂方含药血清组(简称“低、中、高浓度组”)(10.0%、5.0%、2.5%枳葛保肝降脂方含药血清+2.5%无水乙醇)。采用酶联免疫吸附试验法检测各组细胞裂解液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-18含量;Western blot法检测各组Toll样受体4(TLR4)、髓样分化因子88(MyD88)、NLR家族含pyrin结构域蛋白3(NLRP3)、Caspase-1、磷酸化核因子κB p65亚基/核因子κB p65亚基(p-NF-κBp65/NF-κBp65)蛋白表达水平;RT-qPCR检测各组TLR4、MYD88、NLRP3、Caspase-1 m RNA表达情况。结果与正常组比较,诱导组中TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1 m RNA表达量均升高(P<0.01)。与诱导组比较,中、高浓度组TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65mRNA表达量均降低(P<0.01)。结论枳葛保肝降脂方含药血清对无水乙醇诱导的人肝LO2细胞炎症损伤具有保护作用,其作用可能通过TLR4/NF-κB信号通路产生。展开更多
On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction ...On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction of Chengdu.Appropriately,the street was French-styled,and melodious music filled the air.Jean-Jacques Barbaux,president of Seine-展开更多
In this paper,a terahertz slotted waveguide array antenna is designed based on photonic crystal,which can realize efficient radiation of terahertz waves.The electromagnetic wave is fed from the rectangular waveguide a...In this paper,a terahertz slotted waveguide array antenna is designed based on photonic crystal,which can realize efficient radiation of terahertz waves.The electromagnetic wave is fed from the rectangular waveguide at the bottom of the antenna,coupled to photonic crystal waveguide through photonic crystal cavity,and radiated outward through slots at the top layer of antenna.The simulation results show that the antenna achieves a peak gain of 13.45 dBi at 360 GHz,a half-power beam width of 10.9°,and a side lobe level of−13.9 dB.The antenna based on photonic crystal has the advantages of low profile,low loss,and high radiation efficiency,which can be applied to terahertz wireless communication systems.展开更多
BACKGROUND Finding active lead anti-hepatocellular carcinoma compounds from traditional Chinese medicine has important research value.AIM To assess the detailed mechanism of oxocrebanine,a compound separated from the ...BACKGROUND Finding active lead anti-hepatocellular carcinoma compounds from traditional Chinese medicine has important research value.AIM To assess the detailed mechanism of oxocrebanine,a compound separated from the traditional Chinese medicinal plant Stephania hainanensis H.S.Lo et Y.Tsoong,and to evaluate its inhibition of the proliferation of human hepatocellular carcinoma cells via apoptosis and autophagy.METHODS MTT,BrdU labeling,and colony formation assays were used to assess the inhibitory effect of oxocrebanine on the growth and proliferation of human hepatocellular carcinoma Hep3B2.1-7 cells.Flow cytometry was used to detect the effect of oxocrebanine on the apoptosis of Hep3B2.1-7 cells.Western blotting was used to assess the expression of apoptosis-related proteins in Hep3B2.1-7 cells.The aforementioned methods were also used to evaluate the effects of oxocrebanine on cell proliferation,autophagy markers,and autophagy-related protein expression levels after adding autophagy inhibitor 3-mA.Furthermore,to verify the anti-hepatocellular carcinoma effect of oxocrebanine in vivo,a nude mouse model was used to investigate the inhibitory effect of oxocrebanine treatment and its mechanism.Apoptosis was detected using a TUNEL assay and the expression of microtubule-associated protein 1 LC3 in tumor specimens was assessed using immunohistochemistry.RESULTS Oxocrebanine effectively inhibited the growth of Hep3B2.1-7 cells,whilst upregulating the protein expression of cleaved caspase-3,downregulating poly(ADP-ribose)polymerase 1 protein expression,increasing the levels of Bax and Bcl-2 antagonist/killer 1 protein expression,and decreasing the levels of Bcl-2 and myeloid cell leukemia 1 protein expression,which could promote apoptosis in Hep3B2.1-7 cells.Oxocrebanine promoted the transformation of LC3-I to LC3-II in Hep3B2.1-7 cells,suggesting the occurrence of autophagy,whilst the autophagy inhibitor 3-MA could reverse this process.Oxocrebanine was also shown to reduce the phosphorylation levels of the eukaryotic translation initiation factor 4EBP1 and ribosomal protein S6 kinase B1(P70S6K),two downstream effector molecules in the PI3K/Akt/mTOR pathway,inducing autophagy in Hep3B2.1-7 cells.Moreover,the tumor-bearing nude mouse experiment indicated that oxocrebanine effectively inhibited the growth of Hep3B2.1-7 cells in vivo.The results of the TUNEL assay and immunohistochemistry also revealed that oxocrebanine induced apoptosis in vivo and increased the expression level of LC3,an autophagy marker.CONCLUSION Oxocrebanine can inhibit the proliferation of human hepatocellular carcinoma cells by promoting apoptosis and inducing autophagy in vitro and in vivo.展开更多
文摘目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5%无水乙醇)和低、中、高浓度枳葛保肝降脂方含药血清组(简称“低、中、高浓度组”)(10.0%、5.0%、2.5%枳葛保肝降脂方含药血清+2.5%无水乙醇)。采用酶联免疫吸附试验法检测各组细胞裂解液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-18含量;Western blot法检测各组Toll样受体4(TLR4)、髓样分化因子88(MyD88)、NLR家族含pyrin结构域蛋白3(NLRP3)、Caspase-1、磷酸化核因子κB p65亚基/核因子κB p65亚基(p-NF-κBp65/NF-κBp65)蛋白表达水平;RT-qPCR检测各组TLR4、MYD88、NLRP3、Caspase-1 m RNA表达情况。结果与正常组比较,诱导组中TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1 m RNA表达量均升高(P<0.01)。与诱导组比较,中、高浓度组TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65mRNA表达量均降低(P<0.01)。结论枳葛保肝降脂方含药血清对无水乙醇诱导的人肝LO2细胞炎症损伤具有保护作用,其作用可能通过TLR4/NF-κB信号通路产生。
文摘On the morning of Oct 12,2017,a representative office of France’s Seine-et-Marne department in the country’s Ile-de-France region was opened in Bailu Music Townof Pengzhou,a county-level city under the jurisdiction of Chengdu.Appropriately,the street was French-styled,and melodious music filled the air.Jean-Jacques Barbaux,president of Seine-
基金supported by the National Natural Science Foundation of China(No.62375031)the Basic Research Project of Chongqing Science and Technology Commission(No.CSTC-2021jcyj-bsh0194)the Science and Technology Research Program of Chongqing Municipal Education Commission(No.KJQN202200602)。
文摘In this paper,a terahertz slotted waveguide array antenna is designed based on photonic crystal,which can realize efficient radiation of terahertz waves.The electromagnetic wave is fed from the rectangular waveguide at the bottom of the antenna,coupled to photonic crystal waveguide through photonic crystal cavity,and radiated outward through slots at the top layer of antenna.The simulation results show that the antenna achieves a peak gain of 13.45 dBi at 360 GHz,a half-power beam width of 10.9°,and a side lobe level of−13.9 dB.The antenna based on photonic crystal has the advantages of low profile,low loss,and high radiation efficiency,which can be applied to terahertz wireless communication systems.
基金Supported by National Natural Science Foundation of China,No.82060778the Hainan Provincial Natural Science Foundation of China,No.820RC776+1 种基金the Hainan Province Health Science and Technology Innovation Joint Project,No.WSJK2024MS162the Heilongjiang Province Scientific Research Project of Traditional Chinese Medicine,No.ZHY2024-098.
文摘BACKGROUND Finding active lead anti-hepatocellular carcinoma compounds from traditional Chinese medicine has important research value.AIM To assess the detailed mechanism of oxocrebanine,a compound separated from the traditional Chinese medicinal plant Stephania hainanensis H.S.Lo et Y.Tsoong,and to evaluate its inhibition of the proliferation of human hepatocellular carcinoma cells via apoptosis and autophagy.METHODS MTT,BrdU labeling,and colony formation assays were used to assess the inhibitory effect of oxocrebanine on the growth and proliferation of human hepatocellular carcinoma Hep3B2.1-7 cells.Flow cytometry was used to detect the effect of oxocrebanine on the apoptosis of Hep3B2.1-7 cells.Western blotting was used to assess the expression of apoptosis-related proteins in Hep3B2.1-7 cells.The aforementioned methods were also used to evaluate the effects of oxocrebanine on cell proliferation,autophagy markers,and autophagy-related protein expression levels after adding autophagy inhibitor 3-mA.Furthermore,to verify the anti-hepatocellular carcinoma effect of oxocrebanine in vivo,a nude mouse model was used to investigate the inhibitory effect of oxocrebanine treatment and its mechanism.Apoptosis was detected using a TUNEL assay and the expression of microtubule-associated protein 1 LC3 in tumor specimens was assessed using immunohistochemistry.RESULTS Oxocrebanine effectively inhibited the growth of Hep3B2.1-7 cells,whilst upregulating the protein expression of cleaved caspase-3,downregulating poly(ADP-ribose)polymerase 1 protein expression,increasing the levels of Bax and Bcl-2 antagonist/killer 1 protein expression,and decreasing the levels of Bcl-2 and myeloid cell leukemia 1 protein expression,which could promote apoptosis in Hep3B2.1-7 cells.Oxocrebanine promoted the transformation of LC3-I to LC3-II in Hep3B2.1-7 cells,suggesting the occurrence of autophagy,whilst the autophagy inhibitor 3-MA could reverse this process.Oxocrebanine was also shown to reduce the phosphorylation levels of the eukaryotic translation initiation factor 4EBP1 and ribosomal protein S6 kinase B1(P70S6K),two downstream effector molecules in the PI3K/Akt/mTOR pathway,inducing autophagy in Hep3B2.1-7 cells.Moreover,the tumor-bearing nude mouse experiment indicated that oxocrebanine effectively inhibited the growth of Hep3B2.1-7 cells in vivo.The results of the TUNEL assay and immunohistochemistry also revealed that oxocrebanine induced apoptosis in vivo and increased the expression level of LC3,an autophagy marker.CONCLUSION Oxocrebanine can inhibit the proliferation of human hepatocellular carcinoma cells by promoting apoptosis and inducing autophagy in vitro and in vivo.
