[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were ...[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were separated from the aerial part of B.frutescens,immersed in distilled water and treated by ultrasound for half an hour.The volatile oil in B.frutescens leaves was extracted by steam distillation.The leaves after removing the volatile oil were extracted by reflux condensation,and then the extract was concentrated into fluid extract.The organic solvent extraction method was applied to extract the fluid extract until the organic phase was nearly colorless,and the extracts were combined,and concentrated into fluid extract,which was then freeze-dried into powder for later use.A cytotoxicity experiment was carried out on human colon cancer LOVO cells with different extraction fractions of B.frutescens as test drugs.Cell activity was detected by MTT assay.[Results]The petroleum ether fraction and chloroform fraction of B.frutescens had no inhibitory effect on the proliferation of human intestinal cancer LOVO cells in vitro.The ethyl acetate fraction and n-butanol fraction of B.frutescens had inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.The IC_(50)of ethyl acetate was 398.94μg/ml,and that of n-butanol was 617.02μg/ml.[Conclusions]The ethyl acetate and n-butanol extraction fractions from B.frutescens have inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.展开更多
基金Supported by Social Development Program of Guilin Science and Technology Bureau(20210227-9-3)Guangxi Medical and Health Appropriate Technology Development and Application of the Project(S202310601143)University-Level Undergraduate Innovation and Entrepreneurship Training Program(X202410601223).
文摘[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were separated from the aerial part of B.frutescens,immersed in distilled water and treated by ultrasound for half an hour.The volatile oil in B.frutescens leaves was extracted by steam distillation.The leaves after removing the volatile oil were extracted by reflux condensation,and then the extract was concentrated into fluid extract.The organic solvent extraction method was applied to extract the fluid extract until the organic phase was nearly colorless,and the extracts were combined,and concentrated into fluid extract,which was then freeze-dried into powder for later use.A cytotoxicity experiment was carried out on human colon cancer LOVO cells with different extraction fractions of B.frutescens as test drugs.Cell activity was detected by MTT assay.[Results]The petroleum ether fraction and chloroform fraction of B.frutescens had no inhibitory effect on the proliferation of human intestinal cancer LOVO cells in vitro.The ethyl acetate fraction and n-butanol fraction of B.frutescens had inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.The IC_(50)of ethyl acetate was 398.94μg/ml,and that of n-butanol was 617.02μg/ml.[Conclusions]The ethyl acetate and n-butanol extraction fractions from B.frutescens have inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.
