Objective:Acute lung injury(ALI)is a life-threatening respiratory disorder and a major concern in modern medical research.Clinical research has shown that Qingfei Liyan Decoction(QFLYT),a formula based on Yin Qiao San...Objective:Acute lung injury(ALI)is a life-threatening respiratory disorder and a major concern in modern medical research.Clinical research has shown that Qingfei Liyan Decoction(QFLYT),a formula based on Yin Qiao San,is used to treat lung-associated inflammatory diseases.However,the mechanism through which QFLYT exerts its therapeutic effects remains unclear.The purpose of this study was to evaluate the effects of QFLYT on lipopolysaccharide(LPS)-induced ALI and investigate the underlying molecular mechanisms.Materials and Methods:We established an ALI model by administering LPS drops into the trachea of mice.Bronchoalveolar lavage fluid(BALF)and lung tissue were collected to determine inflammatory indices.The effects of QFLYT and its single herbs on the cyclic GMP-AMP synthase-stimulator of interferon gene(cGAS-STING)pathway and inflammasomes were studied in vitro.Relevant proteins and factors were detected using the enzyme linked immunosorbent assay,real-time quantitative polymerase chain reaction,and Western blotting.Results:QFLYT reduced the secretion of inflammatory factors in BALF and lung tissue,and attenuated pathological damage to the latter.In addition,QFLYT inhibited multiple stimuli-induced cGAS-STING pathway and inflammasomes activation in vitro.Further investigation revealed that the herbs in QFLYT had no combined effects on the activation of the two pathways at their formulated doses.However,Forsythiae fructus,Lonicerae japonicae flos,and Licorice inhibited the activation of both pathways at higher concentrations.Conclusions:Our findings show that QFLYT ameliorates LPS-induced ALI by inhibiting the cGAS-STING pathway and inflammasomes.展开更多
Objective： To study the effect of Qingre Liyan Decoction （清热利咽汤 QRLYD） in the prevention and treatment of acute radiative oral mucositis （AROM）, and to explore the mechanism of QRLYD by detecting epidermal g...Objective： To study the effect of Qingre Liyan Decoction （清热利咽汤 QRLYD） in the prevention and treatment of acute radiative oral mucositis （AROM）, and to explore the mechanism of QRLYD by detecting epidermal growth factor （EGF） and T lymphocytes （CD3, CD4, and CD8）. Methods： Sixty patients conforming with the standard were randomly assigned to two groups, 30 patients in each group. Patients in the trial group were treated with QRLYD, and those in the control group were treated with Dobell＇s solution, both groups receiving conventional radiation treatment. The treatment course for both groups was 6 weeks on average. Blood routine test, CD3, CD4, and CD8 in the peripheral blood and EGF in the saliva were detected one day before and on the 14th and 28th day of radio-therapy. Results： Patients in the trial group were in good condition with normal spirits and intake of food and drinks. The incidence of AROM is lower and the effect in preventing AROM is higher in the trial group than those in the control group （P〈0.05）. The EGF in saliva, and CD4 and CD8 in the blood of patients in the trial group were higher than those in the control group （P〈0.05）. Conclusion： QRLYD can cure and prevent AROM. The mechanism may be related with its effects in enhancing body immunity and promoting salivary EGF.展开更多
基金by the National Key R and D Program of China(2023YFC2308200)the Natural Science Foundation of Beijing,China(7232321)+1 种基金the National Natural Science Foundation of China(U23A20519,81930110,82230118)Key project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(2060302)。
文摘Objective:Acute lung injury(ALI)is a life-threatening respiratory disorder and a major concern in modern medical research.Clinical research has shown that Qingfei Liyan Decoction(QFLYT),a formula based on Yin Qiao San,is used to treat lung-associated inflammatory diseases.However,the mechanism through which QFLYT exerts its therapeutic effects remains unclear.The purpose of this study was to evaluate the effects of QFLYT on lipopolysaccharide(LPS)-induced ALI and investigate the underlying molecular mechanisms.Materials and Methods:We established an ALI model by administering LPS drops into the trachea of mice.Bronchoalveolar lavage fluid(BALF)and lung tissue were collected to determine inflammatory indices.The effects of QFLYT and its single herbs on the cyclic GMP-AMP synthase-stimulator of interferon gene(cGAS-STING)pathway and inflammasomes were studied in vitro.Relevant proteins and factors were detected using the enzyme linked immunosorbent assay,real-time quantitative polymerase chain reaction,and Western blotting.Results:QFLYT reduced the secretion of inflammatory factors in BALF and lung tissue,and attenuated pathological damage to the latter.In addition,QFLYT inhibited multiple stimuli-induced cGAS-STING pathway and inflammasomes activation in vitro.Further investigation revealed that the herbs in QFLYT had no combined effects on the activation of the two pathways at their formulated doses.However,Forsythiae fructus,Lonicerae japonicae flos,and Licorice inhibited the activation of both pathways at higher concentrations.Conclusions:Our findings show that QFLYT ameliorates LPS-induced ALI by inhibiting the cGAS-STING pathway and inflammasomes.
基金Supported by the Foundation Item of Shaanxi Provincial Administration of Traditional Chinese Medicine(No.0331)
文摘Objective： To study the effect of Qingre Liyan Decoction （清热利咽汤 QRLYD） in the prevention and treatment of acute radiative oral mucositis （AROM）, and to explore the mechanism of QRLYD by detecting epidermal growth factor （EGF） and T lymphocytes （CD3, CD4, and CD8）. Methods： Sixty patients conforming with the standard were randomly assigned to two groups, 30 patients in each group. Patients in the trial group were treated with QRLYD, and those in the control group were treated with Dobell＇s solution, both groups receiving conventional radiation treatment. The treatment course for both groups was 6 weeks on average. Blood routine test, CD3, CD4, and CD8 in the peripheral blood and EGF in the saliva were detected one day before and on the 14th and 28th day of radio-therapy. Results： Patients in the trial group were in good condition with normal spirits and intake of food and drinks. The incidence of AROM is lower and the effect in preventing AROM is higher in the trial group than those in the control group （P〈0.05）. The EGF in saliva, and CD4 and CD8 in the blood of patients in the trial group were higher than those in the control group （P〈0.05）. Conclusion： QRLYD can cure and prevent AROM. The mechanism may be related with its effects in enhancing body immunity and promoting salivary EGF.
