Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very ...Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.展开更多
Is He Living or Is He Dead is mainly about an outstanding painter Francois Millet who pretend to be dead in order to get reputation and money,because at that time a great artist has never been acknowledged until after...Is He Living or Is He Dead is mainly about an outstanding painter Francois Millet who pretend to be dead in order to get reputation and money,because at that time a great artist has never been acknowledged until after he was starved and dead. Based on the critic approach of New Historicism, this thesis have analyzed how Millet subvert power and be contained by power, the capitalism and market, which criticized the phenomenon that the value of art is not decided by itself, but by power. No matter it is good or bad, if it is denied by power, it is valueless.展开更多
The dynamic differences between a living human brain and a clinically dead (fixed) human brain were measured over international Quantitative Electroencephalographic (QEEG) sites for 1 Hz increments between 1 Hz and 50...The dynamic differences between a living human brain and a clinically dead (fixed) human brain were measured over international Quantitative Electroencephalographic (QEEG) sites for 1 Hz increments between 1 Hz and 50 Hz. Although the expected greater power (μV2•Hz<sup>–</sup><sup>1</sup>) for the living brain was apparent, the difference was particularly obvious for theta and low beta bands. The integrated square root values over the entire band indicated the difference in intrinsic charge-based energy between the living (higher) and dead brain was ~2•10<sup>–</sup><sup>23</sup> J. This quantity is remarkably proximal to the Cosmic Background Microwave value and would be consistent with the Hameroff-Penrose definition of consciousness that suggests a permeating presence derived from discrete physical events. A power value obtained by multiplying this increment of energy by the frequency of the universal hydrogen line resulted in ~10<sup>–</sup><sup>12</sup> W•m<sup>–</sup><sup>2</sup> when applied to the surface area of the human cerebrum. This value is the median flux density that has been measured from human brains during cognition and defines the ultra-weak photon emissions displayed by cells, tissue, and organisms. These results suggest that modern technology may now be sufficiently precise to discern the critical parameters that differentiate the living brain from the fixed “dead” brain. This information might be useful for future designs of virtual consciousness and simulations within cerebral space.展开更多
In the interpretation of Mark Twain’s Is He Living or Is He Dead,the author points out that in the cage of human predic-ament the choice for being or nothingness lies in the fundamental precondition of ensuring subje...In the interpretation of Mark Twain’s Is He Living or Is He Dead,the author points out that in the cage of human predic-ament the choice for being or nothingness lies in the fundamental precondition of ensuring subjectivity first and foremost,with equal emphasis on the organic whole of the signifier and the signified in what he or she is doing,so as to realize the subject’s true value of life.展开更多
基金supported by a Grant-in-Aid for Challenging Exploratory Research(No.15K12192)from the Japan Society for the Promotion of Science(JSPS)The State Scholarship Fund of Chinese Scholarship Council(CSC No.201406010350)provides Mr.Wei Hu the scholarship for his study and stay at the Prefectural University of Kumamoto
文摘Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.
文摘Is He Living or Is He Dead is mainly about an outstanding painter Francois Millet who pretend to be dead in order to get reputation and money,because at that time a great artist has never been acknowledged until after he was starved and dead. Based on the critic approach of New Historicism, this thesis have analyzed how Millet subvert power and be contained by power, the capitalism and market, which criticized the phenomenon that the value of art is not decided by itself, but by power. No matter it is good or bad, if it is denied by power, it is valueless.
文摘The dynamic differences between a living human brain and a clinically dead (fixed) human brain were measured over international Quantitative Electroencephalographic (QEEG) sites for 1 Hz increments between 1 Hz and 50 Hz. Although the expected greater power (μV2•Hz<sup>–</sup><sup>1</sup>) for the living brain was apparent, the difference was particularly obvious for theta and low beta bands. The integrated square root values over the entire band indicated the difference in intrinsic charge-based energy between the living (higher) and dead brain was ~2•10<sup>–</sup><sup>23</sup> J. This quantity is remarkably proximal to the Cosmic Background Microwave value and would be consistent with the Hameroff-Penrose definition of consciousness that suggests a permeating presence derived from discrete physical events. A power value obtained by multiplying this increment of energy by the frequency of the universal hydrogen line resulted in ~10<sup>–</sup><sup>12</sup> W•m<sup>–</sup><sup>2</sup> when applied to the surface area of the human cerebrum. This value is the median flux density that has been measured from human brains during cognition and defines the ultra-weak photon emissions displayed by cells, tissue, and organisms. These results suggest that modern technology may now be sufficiently precise to discern the critical parameters that differentiate the living brain from the fixed “dead” brain. This information might be useful for future designs of virtual consciousness and simulations within cerebral space.
文摘In the interpretation of Mark Twain’s Is He Living or Is He Dead,the author points out that in the cage of human predic-ament the choice for being or nothingness lies in the fundamental precondition of ensuring subjectivity first and foremost,with equal emphasis on the organic whole of the signifier and the signified in what he or she is doing,so as to realize the subject’s true value of life.
