AIM To assess the efficacy and safety of a Chinese herbal medicine (CHM), Xiangsha Liujunzi granules, in the treatment of patients with functional dyspepsia (FD). METHODS We performed a randomized, double-blind, place...AIM To assess the efficacy and safety of a Chinese herbal medicine (CHM), Xiangsha Liujunzi granules, in the treatment of patients with functional dyspepsia (FD). METHODS We performed a randomized, double-blind, placebo-controlled trial with patients from three centers. Two hundred and sixteen subjects diagnosed with FD according to ROME. criteria and confirmed by upper gastrointestinal endoscopy and spleen-deficiency and Qi-stagnation syndrome were selected to receive Xiangsha Liujunzi granules or placebo for 4 wk in a 2: 1 ratio by blocked randomization. The subjects also received follow-up after the 4-wk intervention. Herbal or placebo granules were dissolved in 300 mL of water. Participants in both groups were administered 130 mL (45 degrees C) three times a day. Participants were evaluated prior to and following 4 wk of the intervention in terms of changes in the postprandial discomfort severity scale (PDSS) score, clinical global impression (CGI) scale score, hospital anxiety and depression scale (HADS) score, traditional Chinese medicine symptoms score (SS), scores of various domains of the 36-item short form health survey (SF-36), gastric emptying (GE) and any observed adverse effects. RESULTS Compared with the placebo group, patients in the CHM group showed significant improvements in the scores of PDSS, HADS, SS, SF-36 and CGI scale (P < 0.05 or P < 0.01). They also showed the amelioration in the GE rates of the proximal stomach and distal stomach (P < 0.05 or P < 0.01). CONCLUSION Xiangsha Liujunzi granules offered significant symptomatic improvement in patients with FD.展开更多
Objective:To observe the effect and mechanism of Xiangsha Liujunzi decoction(XSLJZD)drug serum on gastric antrum smooth muscle cells(SMCs)in rats with functional dyspepsia(FD).Methods:Gastric antrum SMCs from rats wit...Objective:To observe the effect and mechanism of Xiangsha Liujunzi decoction(XSLJZD)drug serum on gastric antrum smooth muscle cells(SMCs)in rats with functional dyspepsia(FD).Methods:Gastric antrum SMCs from rats with FD were isolated,cultured,and then divided into six groups as follows:control,model,domperidone,low-dose XSLJZD(LXSLJZD),medium-dose XSLJZD(MXSLJZD),and high-dose XSLJZD(HXSLJZD).Each group was administered the corresponding drug serum for intervention.Drug serum intervention conditions and proliferative activity of SMCs were tested by cholecystokinin octapeptide.Ghrelin,gastrin,somatostatin,and substance P(SP)levels were measured by ELISA.Somatostatin and SP mRNA expression was measured by real-time PCR.Results:A concentration of 10%drug serum for 24 h was decided to be the best intervention condition for later study.The mean optical density value in the model group was lower than that in the control group(P紏.001).Optical density values in the domperidone and HXSLJZD groups were higher than those in the model group(P?.025,P?.032,respectively).Gastrin,SP,and ghrelin levels in the model group were lower(P?.007,P?.037,P?.005,respectively),but somatostatin levels were higher,compared with those in the control group(P?.031).Gastrin,SP,and ghrelin levels in the domperidone,MXSLJZD,and HXSLJZD groups were higher than those in the model group(all P<.05).Somatostatin levels in the four drug-treated groups were lower than those in the model group(P?.002,P?.007,P?.001,P?.009,respectively).SP mRNA levels in the model group were lower than those in the control,domperidone,MXSLJZD,and HXSLJZD groups(P?.037 P?.016,P?.025,P?.002,respectively).Somatostatin mRNA levels in the model group were higher than those in the control and MXSLJZD groups(P紏.042,P紏.035).Conclusions:XSLJZD and domperidone drug serum effectively promote proliferative activity of gastric antrum SMCs in an FD model.The mechanism of this activity may be regulated by gastrointestinal hormones.展开更多
OBJECTIVE:To further clarify the anticancer mechanisms of Liujunzi decoction(六君子汤)and provide possible targets for the treatment of advanced-stage nonsmall cell lung cancer(NSCLC)by re-analyzing differential gene ...OBJECTIVE:To further clarify the anticancer mechanisms of Liujunzi decoction(六君子汤)and provide possible targets for the treatment of advanced-stage nonsmall cell lung cancer(NSCLC)by re-analyzing differential gene expression profile of peripheral blood mononuclear cells(PBMCs)from Liujunzi decoctiontreated NSCLC patients receiving first-line chemotherapy.METHODS:The PBMC gene expression microarray data set GSE61926 was retrieved from a high throughput gene expression database.Differentially expressed genes(DEGs)were screened by paired sample t-test and the multiple ratio method.Gene ontology and Kyoto encyclopedia of genes and genomes(KEGG)pathway analyses were performed using the DAVID database.The protein–protein interaction(PPI)network was constructed using interaction gene library retrieval tools and Cytoscape software.RESULTS:A total of 162 DEGs were identified,with 67 upregulated genes and 95 downregulated genes.The functional distribution of Gene Oncology(GO)genes showed that DEGs were mostly concentrated in extracellular regions,calcium ion binding,and transcriptase activity.KEGG pathway analysis showed that cytokine–cytokine receptor interactions were significantly enriched.PPI network analysis screened out the top 10 central protein-coding genes with the highest nodal degree:IL2,PIWIL4,DICER1,PIWIL2,SAA1,XCL1,IL22RA1,ARHGAP11A,DCP1A,and GDNF.Among them,the central protein-coding gene with the highest node degree was IL2.In addition,the central protein-coding genes with high node degrees and high molecular complex detection(MCODE)scores were PIWIL4,DICER1,PIWIL2,and DCP1A,all of which are related to tumor development.CONCLUSIONS:One signaling pathway and 10 central protein-coding genes related to anticancer mechanisms were screened by re-analysis of GSE61926 data.IL2,PIWIL4,DICER1,PIWIL2,and DCP1 A may have important roles in the mechanism of Liujunzi decoction treatment against NSCLC.Our results suggest that the anticancer mechanism of Liujunzi decoction may be related to gene silencing by RNA and the biological processes of piwi-interacting RNA and other small RNAs.展开更多
OBJECTIVE Cisplatin is a formidable chemotherapy agent widely applying in antineoplastic treatments,but its side effects often limit the clinical usage.Metabolic disorders are one of the side effects induced by cispla...OBJECTIVE Cisplatin is a formidable chemotherapy agent widely applying in antineoplastic treatments,but its side effects often limit the clinical usage.Metabolic disorders are one of the side effects induced by cisplatin,which closely relate to the onset of chemotherapy-induced anorexia(CIA)in cancer patients but lacks effective controls.Liujunzi decoction(LJZD)is a traditional Chinese formula that has a promising effect in treating CIA.However,whether LJZD ameliorates CIA through adjusting cisplatin-induced metabolic disorders remain unknow.The present study evaluated the mechanism of cisplatin-induced metabolic disorders,and the effect of LJZD in ameliorating these disturbances.METHODS 42 male Sprague-Dawley(SD)rats(180-220 g)were randomly divided into 3 groups:normal control group(distilled water+saline),model group(distilled water+cisplatin),LJZD group(4.8 g·kg^(-1)Liujunzi decoction ingredients+cisplatin).Intragastrical administered each drug twice a day(7∶00-19∶00)since day 0 for 4 d,animals were intraperitoneal injected with cisplatin 6 mg·kg^(-1)1 h after administration while normal control groups were injected with same volume of saline.On day 3,each group was anesthetized with pentobarbital sodium 45 mg·kg^(-1)(ip),and blood samples were collected from aorta abdominalis.Then the samples were analyzed using an LC-ESI-MS/MS system.Significantly regulated metabolites between groups were determined by VIP≥1 and absolute Log2FC(fold change)≥1.Identified metabolites were mapped to Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway database using Metaboanalyst 5.0(https://www.metaboanalyst.ca/).RESULTS A total of 133,77 and 32 differential metabolites were filtrated in control vs model,control vs LJZD and model vs LJZD groups respectively.Comparing to control,the levels of hexadecanoic acid(Log2FC=6.3153),linoleic acid(Log2FC=5.3478),and 8,11-icosadienoic acid(Log2FC=5.2342)significantly increased,and the levels of N-acetyl-L-tyrosine(Log2FC=-2.6283),cinnamic acid(Log2FC=-2.3381),N-acetylphenylalanine(Log2FC=-2.2501)significantly decreased in model group.The KEGG pathway enrichments of these metabolites indicated that,cisplatin-induced metabolic disorders by disturbing metabolism pathways such as linoleic acid metabolism,biosynthesis of unsaturated fatty acids,and phenylalanine metabolism,which suggested that the onset of CIA was partly associated with the metabolic disorders of linoleic acid,unsaturated fatty acids,and phenylalanine.Compared to control,treatment of LJZD significantly increased the levels of 4-hydroxytryptamine(Log2FC=12.0186),hexadecanoic acid(Log2FC=5.7412),linoleic acid(Log2FC=5.1877)and significantly decreased the levels of N-acetylmethionine(Log2FC=-1.7317),2-aminoethanesulfinic acid(Log2FC=-1.6578),N-acetyl-L-tyrosine(Log2FC=-1.5355).And comparing to the model group,4-hydroxytryptamine(Log2FC=12.0186),7,12-diketocholic acid(Log2FC=2.0998),N-acetylneuraminic acid(Log2FC=2.0560)markedly increased,and 3-hydroxy-3-methylpentane-1(Log2FC=-1.9202),5-dioic acid(Log2FC=-1.7166),N-isovaleroylglycine,hexanoyl glycine(Log2FC=-1.4958)markedly decreased in LJZD group.It was worth noting that,there were 23 differential metabolites filtrated both in control vs model and model vs LJZD groups,which were the key metabolites of LJZD in treating CIA.Among these 23 common metabolites,there were 16 metabolites excluding the control vs LJZD group,that was,LJZD had no effect in normal rats while being able to ameliorated cisplatin-induced metabolic disorders by regulating these 16 metabolites.Cisplatin-induced downregulation of 11 metabolites such as hydrocinnamic acid,(±)12(13)epoxy-9Z-octadecenoic acid,cinnamic acid were upregulated after LJZD treatment,and cisplatin-induced upregulation of imidazoleacetic acid,2′-deoxycytidine-5′-monophosphate and other 5 metabolites were downregulated by LJZD.The KEGG pathway analysis indicated that the linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism were the most enriched metabolic pathway.Thus,cisplatin-induced metabolic disturbances mainly by disturbing linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism,and LJZD interacted with these metabolic pathways to reduce metabolic disorders and thus ameliorated CIA.CONCLUSION Cisplatin-induced anorexia was closely related to the metabolic disorders of linoleic acid metabolism,biosynthesis of unsaturated fatty acids,and phenylalanine metabolism.The mechanism of LJZD in ameliorating CIA was in concerned with the metabolic adjustments,relating to the regulation of linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism.展开更多
Objective: to study the therapeutic effect of chemotherapy combined with Chaishao Liujunzi decoction on breast cancer. Methods: 130 cases of breast cancer patients admitted to our hospital from September 2020 to Septe...Objective: to study the therapeutic effect of chemotherapy combined with Chaishao Liujunzi decoction on breast cancer. Methods: 130 cases of breast cancer patients admitted to our hospital from September 2020 to September 2021 were randomly divided into control group and experimental group according to the computer method. There were 65 cases in each group, and the patients in the control group received routine chemotherapy and medication. The patients in the experimental group were treated with Chaishao Liujunzi Decoction on the basis of the control group. The curative effect, TCM symptom score before and after the intervention and myelosuppression status (platelet count, white blood cell count and hemoglobin concentration) of the two groups were compared. Results: compared with the effective rate of comprehensive treatment, 64 cases (98.46%) in the experimental group and 58 cases (89.23%) in the control group were comprehensively effective (P < 0.05). Before treatment, there was no significant difference between the two groups (P>0.05). After treatment, the TCM waiting score of the experimental group was better than that of the control group (P<0.05). Before treatment, there was no significant difference in platelet count, leukocyte count and hemoglobin concentration between the two groups (P > 0.05). After treatment, the measurement results of various myelosuppression indexes in the experimental group were significantly better than those in the control group (P < 0.05). The incidence of adverse reactions in the experimental group on the 2nd day of chemotherapy was significantly lower than that in the control group (p < 0.05). Conclusion: the application of Chaishao Liujunzi Decoction in the clinical treatment of breast cancer patients undergoing chemotherapy can improve the patient's symptoms, and the curative effect is ideal. It is recommended to be popularized.展开更多
Objective:To explore the effects and mechanism of Chinese medicine Liujunzi Decoction(LJZD)on regulating microbial flora in mice with asthma flora disorder.Methods:Thirty BALB/c female mice were divided into control,m...Objective:To explore the effects and mechanism of Chinese medicine Liujunzi Decoction(LJZD)on regulating microbial flora in mice with asthma flora disorder.Methods:Thirty BALB/c female mice were divided into control,model,LJZD[3.5 g/(kg·d),by gavage],dexamethasone[DXMS,0.7 mg/(kg·d),intraperitoneal injection],and Clostridium butyricum[CB,230 mg/(kg·d),by gavage]groups according to a random number table,6 mice in each group.The asthma flora disorder mice model was induced with ovalbumin(OVA).Lung and gut lesions were analyzed by hematoxylin-eosin(HE)and periodic acid-Schiff(PAS)stainings.The secretory immunoglobulin A(slgA)protein expression in lung and gut tissues was detected by Western blot.Flow cytometry was used to detect the relative counts of GATA binding protein 3(GATA3)/type 2 innate lymphoid cells(ILC2)in lung and gut.The levels of inflammatory factors in lung and gut tissues were detected by enzyme-linked immunosorbent assay(ELISA).Chao1 and Shannon index were used to compare microbial abundance and diversity in alveolar lavage fluid and cecal contents.The similarity or difference in the composition of mice microbial communities was analyzed through cluster analysis.The serum short-chain fatty acids(SCFAs)content was detected by ultra performance liquid chromatograph mass spectrometer(LC-MS)/MS.Results:The asthma flora disorder model mice showed obvious asthma-related symptoms,but LJZD treatment effectively alleviated these symptoms.LJZD restored alveolar wall thickening,airway inflammatory cell infiltration,gut tissue structure destruction,and inflammatory cell infiltration in asthma flora disorder mice.LJZD downregulated the slgA protein expression in mice(P<0.05).Moreover,LJZD decreased the activation of GATA3/ILC2s in lung and gut tissue(P<0.01),and reduced the levels of interleukin(IL)-5,IL-33,IL-25,IL-9 and IL-13(P<0.01).LJZD treatment returned the abundance of microbial species and the microbial community structure of alveolar lavage fluid and cecal content in asthma flora disorder mice to the normal state.The SCFAs content and body metabolism were also improved.Conclusion:LJZD exerted anti-asthmatic effects by improving the microbial balance of lung-gut axis and affecting systemic metabolism,consequently regulating the GATA3/ILC2s axis to impact the lung inflammatory response.展开更多
基金Supported by the Major State Basic Research Development Program of China(973 Program)No.2013CB531703+1 种基金National Nature Science Foundation of China,No.81503567 and No.81673853the China Postdoctoral Science Foundation,No.2015M1227 and No.2016T90195
文摘AIM To assess the efficacy and safety of a Chinese herbal medicine (CHM), Xiangsha Liujunzi granules, in the treatment of patients with functional dyspepsia (FD). METHODS We performed a randomized, double-blind, placebo-controlled trial with patients from three centers. Two hundred and sixteen subjects diagnosed with FD according to ROME. criteria and confirmed by upper gastrointestinal endoscopy and spleen-deficiency and Qi-stagnation syndrome were selected to receive Xiangsha Liujunzi granules or placebo for 4 wk in a 2: 1 ratio by blocked randomization. The subjects also received follow-up after the 4-wk intervention. Herbal or placebo granules were dissolved in 300 mL of water. Participants in both groups were administered 130 mL (45 degrees C) three times a day. Participants were evaluated prior to and following 4 wk of the intervention in terms of changes in the postprandial discomfort severity scale (PDSS) score, clinical global impression (CGI) scale score, hospital anxiety and depression scale (HADS) score, traditional Chinese medicine symptoms score (SS), scores of various domains of the 36-item short form health survey (SF-36), gastric emptying (GE) and any observed adverse effects. RESULTS Compared with the placebo group, patients in the CHM group showed significant improvements in the scores of PDSS, HADS, SS, SF-36 and CGI scale (P < 0.05 or P < 0.01). They also showed the amelioration in the GE rates of the proximal stomach and distal stomach (P < 0.05 or P < 0.01). CONCLUSION Xiangsha Liujunzi granules offered significant symptomatic improvement in patients with FD.
基金We are grateful for the technical support provided by Dr.Lin Lv and Dr.FengyunWang(Xiyuan Hospital,affiliated with the Chinese Academy of TCM,Beijing,China)and Xin Ma(Beijing University of Chinese Medicine,Beijing,China).
文摘Objective:To observe the effect and mechanism of Xiangsha Liujunzi decoction(XSLJZD)drug serum on gastric antrum smooth muscle cells(SMCs)in rats with functional dyspepsia(FD).Methods:Gastric antrum SMCs from rats with FD were isolated,cultured,and then divided into six groups as follows:control,model,domperidone,low-dose XSLJZD(LXSLJZD),medium-dose XSLJZD(MXSLJZD),and high-dose XSLJZD(HXSLJZD).Each group was administered the corresponding drug serum for intervention.Drug serum intervention conditions and proliferative activity of SMCs were tested by cholecystokinin octapeptide.Ghrelin,gastrin,somatostatin,and substance P(SP)levels were measured by ELISA.Somatostatin and SP mRNA expression was measured by real-time PCR.Results:A concentration of 10%drug serum for 24 h was decided to be the best intervention condition for later study.The mean optical density value in the model group was lower than that in the control group(P紏.001).Optical density values in the domperidone and HXSLJZD groups were higher than those in the model group(P?.025,P?.032,respectively).Gastrin,SP,and ghrelin levels in the model group were lower(P?.007,P?.037,P?.005,respectively),but somatostatin levels were higher,compared with those in the control group(P?.031).Gastrin,SP,and ghrelin levels in the domperidone,MXSLJZD,and HXSLJZD groups were higher than those in the model group(all P<.05).Somatostatin levels in the four drug-treated groups were lower than those in the model group(P?.002,P?.007,P?.001,P?.009,respectively).SP mRNA levels in the model group were lower than those in the control,domperidone,MXSLJZD,and HXSLJZD groups(P?.037 P?.016,P?.025,P?.002,respectively).Somatostatin mRNA levels in the model group were higher than those in the control and MXSLJZD groups(P紏.042,P紏.035).Conclusions:XSLJZD and domperidone drug serum effectively promote proliferative activity of gastric antrum SMCs in an FD model.The mechanism of this activity may be regulated by gastrointestinal hormones.
基金Supported by the National Natural Science Foundation of China(NSFC)(81903270)(Therapeutic Effect and Mechanism of Embryonic Lung Progenitor Cell Transplantation on Silicosis Fibrosis)and(81703987)(Study on the Synergistic Effect and Mechanism of Yiqi Yangyin Jiedu Prescription Combined with Anti-CTLA-4 Mab in Inducing Anti-Lung Cancer Immune Response)Jinan Science and Technology Project(201907019)(Study of Therapy and Mechanisms of Embryonic Lung Stem Cell Transplantation on Silicosis Fibrosis)+4 种基金the Shanghai Science and Technology Commission(19401971200)(a Multicenter Randomized Controlled Study on Improving Prognosis of Patients With StageⅢA Non-Small Cell Lung Cancer By Liu Jiaxiang's Prescription for Qi-Yin Differentiation and Treatment)the Emerging Frontier Technology Joint Research Project of Shanghai Shenkang Hospital Development Center(SHDC12018131)(a Multicenter Randomized Controlled Double-Blind Study on the Prevention and Treatment of Postoperative Recurrence and Metastasis Of StageⅢA Non-Small Cell Lung Cancer By Liu Jiaxiang Qi-Yin Differentiation Method)the Major Project of Scientific Research and Innovation Plan of Shanghai Education Commission(No.2017-01-07-00-10-E00064)(Study on the Mechanism of Feyanning Prescription Against Lung Cancer Growth and Metastasis Derived from Jingqi Theory)Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine(AJ-37)(Clinical Study of Yiqi Yangyin Jiedu Prescription Combined With Oxitinib in the Treatment of Advanced Lung Cancer With T790M Mutation)and(AJ-41)(Clinical Study of“Decubitus Chiropractic Therapy”in the Treatment of Vertebral Artery Type Cervical Spondylosis)National Traditional Chinese Medicine Clinical Research Base Long-Yi-Xue-Zhe Nurturing Plan(LYTD-64)(Mechanism of Reversal of EGFR-Tkis Resistance Induced By HGF in Tumor-Bearing H1975-SCID Mice Based on Huachansu Injection)。
文摘OBJECTIVE:To further clarify the anticancer mechanisms of Liujunzi decoction(六君子汤)and provide possible targets for the treatment of advanced-stage nonsmall cell lung cancer(NSCLC)by re-analyzing differential gene expression profile of peripheral blood mononuclear cells(PBMCs)from Liujunzi decoctiontreated NSCLC patients receiving first-line chemotherapy.METHODS:The PBMC gene expression microarray data set GSE61926 was retrieved from a high throughput gene expression database.Differentially expressed genes(DEGs)were screened by paired sample t-test and the multiple ratio method.Gene ontology and Kyoto encyclopedia of genes and genomes(KEGG)pathway analyses were performed using the DAVID database.The protein–protein interaction(PPI)network was constructed using interaction gene library retrieval tools and Cytoscape software.RESULTS:A total of 162 DEGs were identified,with 67 upregulated genes and 95 downregulated genes.The functional distribution of Gene Oncology(GO)genes showed that DEGs were mostly concentrated in extracellular regions,calcium ion binding,and transcriptase activity.KEGG pathway analysis showed that cytokine–cytokine receptor interactions were significantly enriched.PPI network analysis screened out the top 10 central protein-coding genes with the highest nodal degree:IL2,PIWIL4,DICER1,PIWIL2,SAA1,XCL1,IL22RA1,ARHGAP11A,DCP1A,and GDNF.Among them,the central protein-coding gene with the highest node degree was IL2.In addition,the central protein-coding genes with high node degrees and high molecular complex detection(MCODE)scores were PIWIL4,DICER1,PIWIL2,and DCP1A,all of which are related to tumor development.CONCLUSIONS:One signaling pathway and 10 central protein-coding genes related to anticancer mechanisms were screened by re-analysis of GSE61926 data.IL2,PIWIL4,DICER1,PIWIL2,and DCP1 A may have important roles in the mechanism of Liujunzi decoction treatment against NSCLC.Our results suggest that the anticancer mechanism of Liujunzi decoction may be related to gene silencing by RNA and the biological processes of piwi-interacting RNA and other small RNAs.
基金National Natural Science Foundation of China(82174143)and Scientific Research Foundation of Guangdong Pharmaceutical University(51348136)。
文摘OBJECTIVE Cisplatin is a formidable chemotherapy agent widely applying in antineoplastic treatments,but its side effects often limit the clinical usage.Metabolic disorders are one of the side effects induced by cisplatin,which closely relate to the onset of chemotherapy-induced anorexia(CIA)in cancer patients but lacks effective controls.Liujunzi decoction(LJZD)is a traditional Chinese formula that has a promising effect in treating CIA.However,whether LJZD ameliorates CIA through adjusting cisplatin-induced metabolic disorders remain unknow.The present study evaluated the mechanism of cisplatin-induced metabolic disorders,and the effect of LJZD in ameliorating these disturbances.METHODS 42 male Sprague-Dawley(SD)rats(180-220 g)were randomly divided into 3 groups:normal control group(distilled water+saline),model group(distilled water+cisplatin),LJZD group(4.8 g·kg^(-1)Liujunzi decoction ingredients+cisplatin).Intragastrical administered each drug twice a day(7∶00-19∶00)since day 0 for 4 d,animals were intraperitoneal injected with cisplatin 6 mg·kg^(-1)1 h after administration while normal control groups were injected with same volume of saline.On day 3,each group was anesthetized with pentobarbital sodium 45 mg·kg^(-1)(ip),and blood samples were collected from aorta abdominalis.Then the samples were analyzed using an LC-ESI-MS/MS system.Significantly regulated metabolites between groups were determined by VIP≥1 and absolute Log2FC(fold change)≥1.Identified metabolites were mapped to Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway database using Metaboanalyst 5.0(https://www.metaboanalyst.ca/).RESULTS A total of 133,77 and 32 differential metabolites were filtrated in control vs model,control vs LJZD and model vs LJZD groups respectively.Comparing to control,the levels of hexadecanoic acid(Log2FC=6.3153),linoleic acid(Log2FC=5.3478),and 8,11-icosadienoic acid(Log2FC=5.2342)significantly increased,and the levels of N-acetyl-L-tyrosine(Log2FC=-2.6283),cinnamic acid(Log2FC=-2.3381),N-acetylphenylalanine(Log2FC=-2.2501)significantly decreased in model group.The KEGG pathway enrichments of these metabolites indicated that,cisplatin-induced metabolic disorders by disturbing metabolism pathways such as linoleic acid metabolism,biosynthesis of unsaturated fatty acids,and phenylalanine metabolism,which suggested that the onset of CIA was partly associated with the metabolic disorders of linoleic acid,unsaturated fatty acids,and phenylalanine.Compared to control,treatment of LJZD significantly increased the levels of 4-hydroxytryptamine(Log2FC=12.0186),hexadecanoic acid(Log2FC=5.7412),linoleic acid(Log2FC=5.1877)and significantly decreased the levels of N-acetylmethionine(Log2FC=-1.7317),2-aminoethanesulfinic acid(Log2FC=-1.6578),N-acetyl-L-tyrosine(Log2FC=-1.5355).And comparing to the model group,4-hydroxytryptamine(Log2FC=12.0186),7,12-diketocholic acid(Log2FC=2.0998),N-acetylneuraminic acid(Log2FC=2.0560)markedly increased,and 3-hydroxy-3-methylpentane-1(Log2FC=-1.9202),5-dioic acid(Log2FC=-1.7166),N-isovaleroylglycine,hexanoyl glycine(Log2FC=-1.4958)markedly decreased in LJZD group.It was worth noting that,there were 23 differential metabolites filtrated both in control vs model and model vs LJZD groups,which were the key metabolites of LJZD in treating CIA.Among these 23 common metabolites,there were 16 metabolites excluding the control vs LJZD group,that was,LJZD had no effect in normal rats while being able to ameliorated cisplatin-induced metabolic disorders by regulating these 16 metabolites.Cisplatin-induced downregulation of 11 metabolites such as hydrocinnamic acid,(±)12(13)epoxy-9Z-octadecenoic acid,cinnamic acid were upregulated after LJZD treatment,and cisplatin-induced upregulation of imidazoleacetic acid,2′-deoxycytidine-5′-monophosphate and other 5 metabolites were downregulated by LJZD.The KEGG pathway analysis indicated that the linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism were the most enriched metabolic pathway.Thus,cisplatin-induced metabolic disturbances mainly by disturbing linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism,and LJZD interacted with these metabolic pathways to reduce metabolic disorders and thus ameliorated CIA.CONCLUSION Cisplatin-induced anorexia was closely related to the metabolic disorders of linoleic acid metabolism,biosynthesis of unsaturated fatty acids,and phenylalanine metabolism.The mechanism of LJZD in ameliorating CIA was in concerned with the metabolic adjustments,relating to the regulation of linoleic acid metabolism,histidine metabolism,and pyrimidine metabolism.
文摘Objective: to study the therapeutic effect of chemotherapy combined with Chaishao Liujunzi decoction on breast cancer. Methods: 130 cases of breast cancer patients admitted to our hospital from September 2020 to September 2021 were randomly divided into control group and experimental group according to the computer method. There were 65 cases in each group, and the patients in the control group received routine chemotherapy and medication. The patients in the experimental group were treated with Chaishao Liujunzi Decoction on the basis of the control group. The curative effect, TCM symptom score before and after the intervention and myelosuppression status (platelet count, white blood cell count and hemoglobin concentration) of the two groups were compared. Results: compared with the effective rate of comprehensive treatment, 64 cases (98.46%) in the experimental group and 58 cases (89.23%) in the control group were comprehensively effective (P < 0.05). Before treatment, there was no significant difference between the two groups (P>0.05). After treatment, the TCM waiting score of the experimental group was better than that of the control group (P<0.05). Before treatment, there was no significant difference in platelet count, leukocyte count and hemoglobin concentration between the two groups (P > 0.05). After treatment, the measurement results of various myelosuppression indexes in the experimental group were significantly better than those in the control group (P < 0.05). The incidence of adverse reactions in the experimental group on the 2nd day of chemotherapy was significantly lower than that in the control group (p < 0.05). Conclusion: the application of Chaishao Liujunzi Decoction in the clinical treatment of breast cancer patients undergoing chemotherapy can improve the patient's symptoms, and the curative effect is ideal. It is recommended to be popularized.
基金Supported by Hainan Province Clinical Medical Center(No.QWYH202175)the Natural Science Foundation of Hainan Province(No.822QN485)。
文摘Objective:To explore the effects and mechanism of Chinese medicine Liujunzi Decoction(LJZD)on regulating microbial flora in mice with asthma flora disorder.Methods:Thirty BALB/c female mice were divided into control,model,LJZD[3.5 g/(kg·d),by gavage],dexamethasone[DXMS,0.7 mg/(kg·d),intraperitoneal injection],and Clostridium butyricum[CB,230 mg/(kg·d),by gavage]groups according to a random number table,6 mice in each group.The asthma flora disorder mice model was induced with ovalbumin(OVA).Lung and gut lesions were analyzed by hematoxylin-eosin(HE)and periodic acid-Schiff(PAS)stainings.The secretory immunoglobulin A(slgA)protein expression in lung and gut tissues was detected by Western blot.Flow cytometry was used to detect the relative counts of GATA binding protein 3(GATA3)/type 2 innate lymphoid cells(ILC2)in lung and gut.The levels of inflammatory factors in lung and gut tissues were detected by enzyme-linked immunosorbent assay(ELISA).Chao1 and Shannon index were used to compare microbial abundance and diversity in alveolar lavage fluid and cecal contents.The similarity or difference in the composition of mice microbial communities was analyzed through cluster analysis.The serum short-chain fatty acids(SCFAs)content was detected by ultra performance liquid chromatograph mass spectrometer(LC-MS)/MS.Results:The asthma flora disorder model mice showed obvious asthma-related symptoms,but LJZD treatment effectively alleviated these symptoms.LJZD restored alveolar wall thickening,airway inflammatory cell infiltration,gut tissue structure destruction,and inflammatory cell infiltration in asthma flora disorder mice.LJZD downregulated the slgA protein expression in mice(P<0.05).Moreover,LJZD decreased the activation of GATA3/ILC2s in lung and gut tissue(P<0.01),and reduced the levels of interleukin(IL)-5,IL-33,IL-25,IL-9 and IL-13(P<0.01).LJZD treatment returned the abundance of microbial species and the microbial community structure of alveolar lavage fluid and cecal content in asthma flora disorder mice to the normal state.The SCFAs content and body metabolism were also improved.Conclusion:LJZD exerted anti-asthmatic effects by improving the microbial balance of lung-gut axis and affecting systemic metabolism,consequently regulating the GATA3/ILC2s axis to impact the lung inflammatory response.
