Ultrasonic-assisted extraction(UAE)combined with medium pressure liquid chromatography(MPLC)was designed for carbazole separation from anthracene slag(AS).The effects of liquid/solid ratio,temperature,and extraction t...Ultrasonic-assisted extraction(UAE)combined with medium pressure liquid chromatography(MPLC)was designed for carbazole separation from anthracene slag(AS).The effects of liquid/solid ratio,temperature,and extraction times on carbazole separation were investigated.When using CC14 and ethyl acetate as extraction solvents and combining with MPLC,carbazole recovery and purity are 75.1%and 95.4%,respectively.The mechanism for carbazole separation were presumed by examining intermolecular interactions such as N-H…π,π-π,and C-Cl…πinteractions.These results demonstrate that UAE/MPLC has a considerable potential as a green and promising strategy for separating and purifying carbazole and other chemicals from AS.展开更多
It becomes increasingly clear that non-uniform distribution of immiscible fluids in porous rock is particularly relevant to seismic wave dispersion. White proposed a patchy saturation model in 1975, in which spherical...It becomes increasingly clear that non-uniform distribution of immiscible fluids in porous rock is particularly relevant to seismic wave dispersion. White proposed a patchy saturation model in 1975, in which spherical gas pockets were located at the center of a liquid saturated cube. For an extremely light and compressible inner gas, the physical properties can be approximated by a vacuum with White＇s model. The model successfully analyzes the dispersion phenomena of a P-wave velocity in gas-water- saturated rocks. In the case of liquid pocket saturation, e.g., an oil-pocket surrounded by a water saturated host matrix, the light fluid-pocket assumption is doubtful, and few works have been reported in White＇s framework. In this work, Poisson＇s ratio, the bulk modulus, and the effective density of a dual-liquid saturated medium are formulated for the heterogeneous porous rocks containing liquid-pockets. The analysis of the difference between the newly derived bulk modulus and that of White＇s model shows that the effects of liquid-pocket saturation do not disappear unless the porosity approaches zero. The inner pocket fluid can no longer be ignored. The improvements of the P-wave velocity predictions are illustrated with two examples taken from experiments, i.e., the P-wave velocity in the sandstone saturated by oil and brine and the P-wave velocity for heavy oils and stones at different temperatures.展开更多
Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium t...Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid 1/2 MS modified medium supplemented with 4.44 μM 6-Benzyladenine(BA) and 16.1 μM 1-Naphthaleneacetic acid(NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 μM NAA. Shoot multiplication was carried out on 4.44 μM BA + 0.5 μM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation.Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid-IBA(5 and16 μM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 μM BA+0.5 μM NAA resulted in the most shoots/callus.Non-aerated liquid medium was more efficient in promoting shoot multiplication(53.5 shoots/callus) than was semisolid medium(28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting(76%) was obtained using 16 μM IBA.展开更多
The role of the Cattaneo-Christov heat flux theory in the two-dimensional laminar flow of the Jeffrey liquid is discussed with a vertical sheet. The salient feature in the energy equation is accounted due to the imple...The role of the Cattaneo-Christov heat flux theory in the two-dimensional laminar flow of the Jeffrey liquid is discussed with a vertical sheet. The salient feature in the energy equation is accounted due to the implementation of the Cattaneo-Christov heat flux. A liquid with variable thermal conductivity is considered in the Darcy-Forchheimer porous space. The mathematical expressions of momentum and energy are coupled due to the presence of mixed convection. A highly nonlinear coupled system of equations is tackled with the homotopic algorithm. The convergence of the homotopy expressions is calculated graphically and numerically. The solutions of the velocity and temperature are expressed for various values of the Deborah number, the ratio of the relaxation time to the retardation time, the porosity parameter, the mixed convective parameter, the Darcy-Forchheimer parameter, and the conductivity parameter. The results show that the velocity and temperature are higher in Fourier＇s law of heat conduction cases in comparison with the Cattaneo-Christov heat flux model.展开更多
[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different ca...[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.展开更多
Excellent yields and purity were obtained in the aqueous medium Suzuki, Sonogashira, Stille and Heck reactions using palladium (II) as catalyst in liquid phase synthesis. Polyethylene glycol (PEG) acted as soluble pol...Excellent yields and purity were obtained in the aqueous medium Suzuki, Sonogashira, Stille and Heck reactions using palladium (II) as catalyst in liquid phase synthesis. Polyethylene glycol (PEG) acted as soluble polymeric support and phase transfer catalyst as well.展开更多
A highly sensitive and precise method for the determination of the polyamines (including putrescine, spermidine and spermine) in cell culture media is described. The samples were concentrated by C18 column and the pol...A highly sensitive and precise method for the determination of the polyamines (including putrescine, spermidine and spermine) in cell culture media is described. The samples were concentrated by C18 column and the polyamines were converted to fluorometric derivatives with DNS-Cl. The polyamine derivatives were then completely separated by HPLC and determined by simultaneous fluorometric detection. The CV of intragroup and intergroup were 2. 49 %-4. 26% and 4. 29 %-5. 16 %, respectively. The rate of recovery was 103%- 99%. There was trace amount of polyamines detected by this method in the media of F12, 8900, RPMI1640, DMEM and M199 even without incubation with cells. So this method can be used for detecting the changes of polyamines in a medium before and after incubation with cells. It is helpful for the researches on the regulation of cell proliferation by polyamines.展开更多
Lower temperature waste heats less than 373 K have strong potentials to supply additional energies because of their enormous quantities and ubiquity. Accordingly, reinforcement of power generations harvesting low temp...Lower temperature waste heats less than 373 K have strong potentials to supply additional energies because of their enormous quantities and ubiquity. Accordingly, reinforcement of power generations harvesting low temperature heats is one of the urgent tasks for the current generation in order to accomplish energy sustainability in the coming decades. In this study, a liquid turbine power generator driven by lower temperature heats below 373 K was proposed in the aim of expanding selectable options for harvesting low temperature waste heats less than 373 K. The proposing system was so simply that it was mainly composed of a liquid turbine, a liquid container with a biphasic medium of water and an underlying water-insoluble low-boiling-point medium in a liquid phase, a heating section for vaporization of the liquid and a cooling section for entropy discharge outside the system. Assumed power generating steps via the proposing liquid turbine power generator were as follows: step 1: the underlying low-boiling-point medium in a liquid phase was vaporized, step 2: the surfacing vapor bubbles of low-boiling-point medium accompanied the biphasic medium in their wakes, step 3: such high momentum flux by step 2 rotated the liquid turbine (i.e. power generation), step 4: the surfacing low-boiling-point medium vapor was gradually condensed into droplets, step 5: the low-boiling-point medium droplets were submerged to the underlying medium in a liquid phase. Experiments with a prototype liquid turbine power generator proved power generations in accordance with the assumed steps at a little higher than ordinary temperature. Increasing output voltage could be obtained with an increase in the cooling temperature among tested ranging from 294 to 296 K in contrast to normal thermal engines. Further improvements of the direct current voltage from the proposing liquid turbine power generator can be expected by means of far more vigorous multiphase flow induced by adding solid powders and theoretical optimizations of heat and mass transfers.展开更多
基金Supported by the Key Project of Joint Fund for the research on Coal-Based Low Carbon Technology from National Natural Science Foundation of China and the Government of Shanxi(U1610223)Coal-based Key Scientific and Technological Projects of Shanxi Province(MJH2014-15)the National Key Research and Development Program of China(2018YFB0604602).
文摘Ultrasonic-assisted extraction(UAE)combined with medium pressure liquid chromatography(MPLC)was designed for carbazole separation from anthracene slag(AS).The effects of liquid/solid ratio,temperature,and extraction times on carbazole separation were investigated.When using CC14 and ethyl acetate as extraction solvents and combining with MPLC,carbazole recovery and purity are 75.1%and 95.4%,respectively.The mechanism for carbazole separation were presumed by examining intermolecular interactions such as N-H…π,π-π,and C-Cl…πinteractions.These results demonstrate that UAE/MPLC has a considerable potential as a green and promising strategy for separating and purifying carbazole and other chemicals from AS.
基金Project supported by the Open Foundation of SINOPEC Key Laboratory of Geophysics(No.WTYJY-WX2013-04-02)the National Key Basic Research Program of China(973 Program)(No.2014CB239006)the 12th 5-Year Basic Research Program of China National Packaging Corporation(CNPC)(No.2014A-3611)
文摘It becomes increasingly clear that non-uniform distribution of immiscible fluids in porous rock is particularly relevant to seismic wave dispersion. White proposed a patchy saturation model in 1975, in which spherical gas pockets were located at the center of a liquid saturated cube. For an extremely light and compressible inner gas, the physical properties can be approximated by a vacuum with White＇s model. The model successfully analyzes the dispersion phenomena of a P-wave velocity in gas-water- saturated rocks. In the case of liquid pocket saturation, e.g., an oil-pocket surrounded by a water saturated host matrix, the light fluid-pocket assumption is doubtful, and few works have been reported in White＇s framework. In this work, Poisson＇s ratio, the bulk modulus, and the effective density of a dual-liquid saturated medium are formulated for the heterogeneous porous rocks containing liquid-pockets. The analysis of the difference between the newly derived bulk modulus and that of White＇s model shows that the effects of liquid-pocket saturation do not disappear unless the porosity approaches zero. The inner pocket fluid can no longer be ignored. The improvements of the P-wave velocity predictions are illustrated with two examples taken from experiments, i.e., the P-wave velocity in the sandstone saturated by oil and brine and the P-wave velocity for heavy oils and stones at different temperatures.
基金supported by the National Council for Scientific and Technological Development(CNPq)/Brazil,under Grant 477538/2013-4
文摘Eucalyptus is very recalcitrant to in vitro culture.In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid 1/2 MS modified medium supplemented with 4.44 μM 6-Benzyladenine(BA) and 16.1 μM 1-Naphthaleneacetic acid(NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 μM NAA. Shoot multiplication was carried out on 4.44 μM BA + 0.5 μM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation.Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid-IBA(5 and16 μM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 μM BA+0.5 μM NAA resulted in the most shoots/callus.Non-aerated liquid medium was more efficient in promoting shoot multiplication(53.5 shoots/callus) than was semisolid medium(28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting(76%) was obtained using 16 μM IBA.
文摘The role of the Cattaneo-Christov heat flux theory in the two-dimensional laminar flow of the Jeffrey liquid is discussed with a vertical sheet. The salient feature in the energy equation is accounted due to the implementation of the Cattaneo-Christov heat flux. A liquid with variable thermal conductivity is considered in the Darcy-Forchheimer porous space. The mathematical expressions of momentum and energy are coupled due to the presence of mixed convection. A highly nonlinear coupled system of equations is tackled with the homotopic algorithm. The convergence of the homotopy expressions is calculated graphically and numerically. The solutions of the velocity and temperature are expressed for various values of the Deborah number, the ratio of the relaxation time to the retardation time, the porosity parameter, the mixed convective parameter, the Darcy-Forchheimer parameter, and the conductivity parameter. The results show that the velocity and temperature are higher in Fourier＇s law of heat conduction cases in comparison with the Cattaneo-Christov heat flux model.
基金Supported by"Six Talents Peaks"Project in Jiangsu Province in 2015(NY-024)Project of Jiangsu Polytechnic College of Agriculture and Forestry(2014KJ28)
文摘[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.
文摘Excellent yields and purity were obtained in the aqueous medium Suzuki, Sonogashira, Stille and Heck reactions using palladium (II) as catalyst in liquid phase synthesis. Polyethylene glycol (PEG) acted as soluble polymeric support and phase transfer catalyst as well.
文摘A highly sensitive and precise method for the determination of the polyamines (including putrescine, spermidine and spermine) in cell culture media is described. The samples were concentrated by C18 column and the polyamines were converted to fluorometric derivatives with DNS-Cl. The polyamine derivatives were then completely separated by HPLC and determined by simultaneous fluorometric detection. The CV of intragroup and intergroup were 2. 49 %-4. 26% and 4. 29 %-5. 16 %, respectively. The rate of recovery was 103%- 99%. There was trace amount of polyamines detected by this method in the media of F12, 8900, RPMI1640, DMEM and M199 even without incubation with cells. So this method can be used for detecting the changes of polyamines in a medium before and after incubation with cells. It is helpful for the researches on the regulation of cell proliferation by polyamines.
文摘Lower temperature waste heats less than 373 K have strong potentials to supply additional energies because of their enormous quantities and ubiquity. Accordingly, reinforcement of power generations harvesting low temperature heats is one of the urgent tasks for the current generation in order to accomplish energy sustainability in the coming decades. In this study, a liquid turbine power generator driven by lower temperature heats below 373 K was proposed in the aim of expanding selectable options for harvesting low temperature waste heats less than 373 K. The proposing system was so simply that it was mainly composed of a liquid turbine, a liquid container with a biphasic medium of water and an underlying water-insoluble low-boiling-point medium in a liquid phase, a heating section for vaporization of the liquid and a cooling section for entropy discharge outside the system. Assumed power generating steps via the proposing liquid turbine power generator were as follows: step 1: the underlying low-boiling-point medium in a liquid phase was vaporized, step 2: the surfacing vapor bubbles of low-boiling-point medium accompanied the biphasic medium in their wakes, step 3: such high momentum flux by step 2 rotated the liquid turbine (i.e. power generation), step 4: the surfacing low-boiling-point medium vapor was gradually condensed into droplets, step 5: the low-boiling-point medium droplets were submerged to the underlying medium in a liquid phase. Experiments with a prototype liquid turbine power generator proved power generations in accordance with the assumed steps at a little higher than ordinary temperature. Increasing output voltage could be obtained with an increase in the cooling temperature among tested ranging from 294 to 296 K in contrast to normal thermal engines. Further improvements of the direct current voltage from the proposing liquid turbine power generator can be expected by means of far more vigorous multiphase flow induced by adding solid powders and theoretical optimizations of heat and mass transfers.
