Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and dru...Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and drug release rates at different p H values to assess the biodistribution and drug delivery pathways in vivo. Here, we established a strategy to integrate Bi-doped carbon quantum dots(CQDs)with liposomes to produce fluorescence visualization and therapeutic effects, namely lipo/Bi-doped CQDs.Lipo/Bi-doped CQDs show good water solubility and physicochemical properties, which can be used for in vitro labeling of colon cancer(CT26) cells and in vivo imaging localization tracking tumors for monitoring. Simultaneously, thanks to the excellent p H sensitivity and ion doping characteristic of Bi-doped CQDs, lipo/Bi-doped CQDs can be used to reveal the drug release rate of liposomes at different p H values and exhibit potential effects in vivo antitumor therapy.展开更多
The electronic and crystal structural properties of Bi-doped Sr3Ti2O7 are studied using the first principles density functional theory（DFT）based on pseudopotentials basis and plane-wave method.Our results show that ...The electronic and crystal structural properties of Bi-doped Sr3Ti2O7 are studied using the first principles density functional theory（DFT）based on pseudopotentials basis and plane-wave method.Our results show that the formation energy of Bi doping in Site-1 and Site-2 of Sr3Ti2O7 increases with increasing doping concentration.And at the same doping concentration,the formation energy of Bi doping in Site-2 is lower than that in Site-1.The undoped Sr3Ti2O7is found to be an insulator and its Fermi level stays at the top of the valence band.While the Fermi level of the Bi-doped Sr3Ti2O7moves into the bottom of conduction band,the system undergoes an insulator-to-metal phase transition.Furthermore,our calculation results demonstrated that the Fermi level of the Bi-doped Sr3Ti2O7goes deeper into the bottom of conduction band with increasing doping concentration.展开更多
目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌...目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌注(进行肺灌洗及保存)。两组都在4℃保存2 h后再灌注1 h,测定经肺氧合后动脉血氧分压值(PaO2)和肺气道峰压值(PawP)。于再灌注结束后取右上肺组织,测其湿重(Wr)与干重(Wd)、计算湿/干重比(Wr/Wd);ELISA法检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量;免疫组化法检测NF-κB的表达;并用光学显微镜和透射电子显微镜观察再灌注后肺组织结构的病理变化。结果:再灌注30 m in后,两组的PaO2逐渐降低,PawP逐渐升高,实验组PaO2降低程度及PawP的升高程度均低于对照组(P<0.01);实验组的SOD明显高于对照组,MDA及肺组织的NF-κB的表达低于对照组(P<0.01),肺组织的病理变化较对照组轻。结论:Lipo-PGE1注射液能减轻无心跳兔肺缺血/再灌注损伤,改善肺功能,具有肺保护功能。展开更多
基金funded by Beijing Natural Science Foundation (Nos.L222109, 3222018)Military Health Care Project(No.22BJZ22)+6 种基金Science Foundation of China University of Petroleum (Nos.2462019QNXZ02, 2462019BJRC007)National Natural Science Foundation of China (Nos.52211530034, 82273236)Guangdong Provincial Basic and Applied Basic Research Foundation (Nos.2022A151522004, 2022A1515220042)Science and Technology Innovation Commission of Shenzhen (Nos.JSGG20210802153410031, JCYJ20220530141609021)Science and Technology Plan of Shenzhen Nanshan District (No.NS016)Discipline Leader Foundation of Huazhong University of Science and Technology Union Shenzhen Hospital (No.YN2021002)Crosswise Project of Daan Gene (No.HXKY2022002)。
文摘Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and drug release rates at different p H values to assess the biodistribution and drug delivery pathways in vivo. Here, we established a strategy to integrate Bi-doped carbon quantum dots(CQDs)with liposomes to produce fluorescence visualization and therapeutic effects, namely lipo/Bi-doped CQDs.Lipo/Bi-doped CQDs show good water solubility and physicochemical properties, which can be used for in vitro labeling of colon cancer(CT26) cells and in vivo imaging localization tracking tumors for monitoring. Simultaneously, thanks to the excellent p H sensitivity and ion doping characteristic of Bi-doped CQDs, lipo/Bi-doped CQDs can be used to reveal the drug release rate of liposomes at different p H values and exhibit potential effects in vivo antitumor therapy.
基金supported by the National Natural Science Foundation of China (NSFC) (Grant Nos.51575452,51475378 and 51474176)the Fundamental Research Funds for the Central Universities (No.3102015ZY025)+2 种基金the Research Fund of the State Key Laboratory of Solidification Processing,Northwestern Polytechnic University (Grant No.161-QP-2016)NSFC-Guangdong mutual funds (phase ii) supercomputing science and applied research under special fundingNational supercomputing center in Guangzhou
文摘The electronic and crystal structural properties of Bi-doped Sr3Ti2O7 are studied using the first principles density functional theory（DFT）based on pseudopotentials basis and plane-wave method.Our results show that the formation energy of Bi doping in Site-1 and Site-2 of Sr3Ti2O7 increases with increasing doping concentration.And at the same doping concentration,the formation energy of Bi doping in Site-2 is lower than that in Site-1.The undoped Sr3Ti2O7is found to be an insulator and its Fermi level stays at the top of the valence band.While the Fermi level of the Bi-doped Sr3Ti2O7moves into the bottom of conduction band,the system undergoes an insulator-to-metal phase transition.Furthermore,our calculation results demonstrated that the Fermi level of the Bi-doped Sr3Ti2O7goes deeper into the bottom of conduction band with increasing doping concentration.
文摘目的:研究Lipo-PGE1注射液对无心跳兔肺缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:将16只健康新西兰大白兔随机平均分为2组:对照组以低钾右旋糖酐(LPD)液进行肺灌洗及保存;试验组采用Lipo-PGE1注射液(20μg.L-1)加LPD液灌注(进行肺灌洗及保存)。两组都在4℃保存2 h后再灌注1 h,测定经肺氧合后动脉血氧分压值(PaO2)和肺气道峰压值(PawP)。于再灌注结束后取右上肺组织,测其湿重(Wr)与干重(Wd)、计算湿/干重比(Wr/Wd);ELISA法检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量;免疫组化法检测NF-κB的表达;并用光学显微镜和透射电子显微镜观察再灌注后肺组织结构的病理变化。结果:再灌注30 m in后,两组的PaO2逐渐降低,PawP逐渐升高,实验组PaO2降低程度及PawP的升高程度均低于对照组(P<0.01);实验组的SOD明显高于对照组,MDA及肺组织的NF-κB的表达低于对照组(P<0.01),肺组织的病理变化较对照组轻。结论:Lipo-PGE1注射液能减轻无心跳兔肺缺血/再灌注损伤,改善肺功能,具有肺保护功能。
