A new sterile line UP-3s, which carries the Dominant Early Maturity Gene (DEMG), was bred on the farm of University of Arkansas at Pine Bluff (UAPB). UP-3s and two check sterile lines, Jin23-A and Xie-A which do not c...A new sterile line UP-3s, which carries the Dominant Early Maturity Gene (DEMG), was bred on the farm of University of Arkansas at Pine Bluff (UAPB). UP-3s and two check sterile lines, Jin23-A and Xie-A which do not carry the Dominant Early Maturity Gene, were crossed with a group of different maturity restorer lines, PB-1R, PB-5R,PB11, PB-13R, PB-20, PB-21, PB-22R, and PB-23R. Eighteen new hybrid rice combinations of these crosses were then tested at UAPB in 2012 and 2013. The results showed that panicle differentiation (PD) of hybrids from female parent UP-3s (DEMG) crossed with the 8 male parents, were earlier than the hybrids from female parent Jin23-A or Xie-A crossed with the 8 male parents. The PD of these earlier hybrids was before Jun 25 and heading was before July 20. Early PD and heading avoided the high temperature (over 34°C) period which usually occurs after July 20 in Arkansas. The yields of these earlier maturity hybrids with female parent UP-3s were higher than those of the late maturity hybrids thatwereF1 progeny of sterile lines Jin23-A or Xie-A (these two female parent checks with non-DEMG). These results showed that the DEMG sterile line UP-3s can be adopted in making crosses with later maturity restorer lines to obtain earlier maturity hybrids to avoid the high temperature period in Arkansas.展开更多
The voltage uprating of existing overhead lines is an interesting solution for increasing the transmission of electricity, especially in areas where it is difficult to build new lines. If a line is uprated with minor ...The voltage uprating of existing overhead lines is an interesting solution for increasing the transmission of electricity, especially in areas where it is difficult to build new lines. If a line is uprated with minor changes of its design and without improvement of the grounding electrodes of towers, its lightning performance remains unchanged. The consequence is that the uprated line will have a lightning flashover rate very high compared to an overhead line of same nominal voltage but of standard design. One attractive solution to solve this problem is to use line arresters. The goal of this paper is to study the use of line arresters to achieve a satisfactory lightning performance of an overhead line uprated from 225 kV to 400 kV without major design modifications. In order to compare different strategies of line arrester installation the flashover rate is calculated based on a software able to launch automatically EMTP-RV. The software named LIPS has been developed under the cover of a partnership between Hydro-Quebec, RTE and EDF.展开更多
Random integration of massive distributed photovoltaic(PV)generation poses serious challenges to distribution networks.Voltage violations,line overloads,increased peak-valley differences,and power-flow reversals can o...Random integration of massive distributed photovoltaic(PV)generation poses serious challenges to distribution networks.Voltage violations,line overloads,increased peak-valley differences,and power-flow reversals can occur at different locations,times,and severities.Traditional planning methods such as energy storage(ES)allocation and upgrading of lines may result in poor economics and low equipment utilization.This study proposes a distribution-network planning strategy that coordinates three planning mechanisms:ES allocation to substations and to feeders,and line upgrading.The proposed strategy achieved the lowest annual cost,considering the distribution network problems at different locations,times,and severities.A net load curve description method was first proposed considering economic planning and the uncertain distribution of loads and PVs.Second,a multistage optimization model for ES allocation and line upgrading was established based on the net load curves.Finally,an improved IEEE33 node system was used in the case study.The planning results show that the proposed coordinated planning strategy can address the problems caused by the random integration of massive distributed PVs,improve equipment utilization,and obtain the lowest total annual planning cost.展开更多
Leopard coral grouper(Plectropomus leopardus)is a commercially important marine fish species.It is important to study how to prevent it from infecting with various viruses.In this study,we established and characterize...Leopard coral grouper(Plectropomus leopardus)is a commercially important marine fish species.It is important to study how to prevent it from infecting with various viruses.In this study,we established and characterized a new cell line derived from the fin tissue of leopard coral grouper(PLF).The PLF cells were cultured for more than 55 passages.Cytochrome B gene sequencing confirmed the origin of the PLF cells is P.leopardus.Immunostaining against cytokeratin indicated that the PLF cells predominantly consist of epithelial cells.The chromosome number of PLF was 48.The cells grew well in Dulbecco's modified Eagle's medium(DMEM)supplemented with 10%–20%fetal bovine serum(FBS)at temperature between 20–28℃,with the highest growth rate at28℃.Transfection with pEGFP-N3 plasmid showed the transfection efficiency was about 35%.Virus susceptibility tests revealed that PLF cells are susceptible to red-spotted grouper nervous necrosis virus(NNV)and viral hemorrhagic septicemia virus(VHSV),and viral proliferation was confirmed by qRT-PCR and western blot.The altered expressions of immune-related genes TBK1,IRF3,and Mx after NNV and VHSV infections suggested that PLF cells can mount an immune response to fish viruses.Thus the PLF cells can be employed for studying virus-host interactions and developing antiviral strategies.展开更多
The published article titled“Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24”has been retracted from Oncology Research,Vol.26,No.8,2018,pp.1227–1234.
The published article titled“CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line”has been retracted from Oncology Research,Vol.24,No.6,2016,pp....The published article titled“CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line”has been retracted from Oncology Research,Vol.24,No.6,2016,pp.487–494.展开更多
To ensure the safety of power energy transmission channel and mitigate the harm caused by galloping of iced transmission lines,the axial time-delay velocity feedback strategy is adopted to suppress the galloping.The p...To ensure the safety of power energy transmission channel and mitigate the harm caused by galloping of iced transmission lines,the axial time-delay velocity feedback strategy is adopted to suppress the galloping.The par-tial differential equation of galloping with axial time-delay velocity feedback strategy is established based on the variational principle for Hamiltonian.Then,the partial differential equation of galloping is transformed into or-dinary differential equation based on normalization and the Galerkin method.The primary amplitude-frequency response equation,the first-order steady-state approximate solution,and the harmonic amplitude-frequency re-sponse equation are derived by the multiscale method.The impact of different parameters such as time-delay value,control coefficient,and amplitude of external excitation on the galloping response are analyzed.The am-plitude under the primary resonance exhibits periodicity as time-delay value varies.The amplitude diminishes with increased control coefficient and increases with external excitation.Comprehensive consideration of vari-ous influences of parameters on vibration characteristics is crucial when employing the axial time-delay velocity feedback strategy to suppress galloping.Therefore,to achieve the best vibration suppression effect,it is crucial to adjust the time-delay parameter for modifying the range and amplitude of the resonance zone.The conclusions obtained by this study are expected to advance the refinement of active control techniques for iced transmission lines,and may provide valuable insights for practical engineering applications.展开更多
China International Supply Chain Expo(hereinafter referred to as the CISCE)is a key platform for enterprises to showcase their innovation strength and connect with global resources.As a“hidden champion”in the field ...China International Supply Chain Expo(hereinafter referred to as the CISCE)is a key platform for enterprises to showcase their innovation strength and connect with global resources.As a“hidden champion”in the field of intelligent manufacturing,Hunan SpeedBot Robot ics Co.,Ltd.(hereinaf ter refer red to as“SpeedBot”)is planning to display its independently developed“AI+3D Vision”core technology at the CISCE,to empower the supply chain of industries such as automobiles and heavy industry through a full-process automated detection solution.Liu Tingting,Vice General Manager of SpeedBot,said in an interview with China’s Foreign Trade,“The CISCE is not only a window for technology display,but also a link to promote collaborative innovations of the whole industrial chain.We look forward to building resilient supply chains with global partners and bringing China’s‘smart’manufacturing to the world.”展开更多
Over the next 20 years,China's urban rail transit(hereinafter referred to as'urban rail')will face large-scalerenovation of existing line facilities and equipment,with more than 1000 km of renovated lines ...Over the next 20 years,China's urban rail transit(hereinafter referred to as'urban rail')will face large-scalerenovation of existing line facilities and equipment,with more than 1000 km of renovated lines to be added eachyear.In 2024,the China Association of Metros issued the Guiding Opinions on the Renovation of Existing UrbanRail Transit Lines in China,providing guiding opinions on norms,standards,and implementation approaches forthe renovation of existing lines in the coming period.In the practical work of renovating existing urban rail lines,it is necessary to continuously explore and refine relevant theoretical methods in line with industry developmenttrends and urban development requirements.The following are the author's recent reflections on theoreticalinnovation in this field.展开更多
Aircraft assembly is characterized by stringent precedence constraints,limited resource availability,spatial restrictions,and a high degree of manual intervention.These factors lead to considerable variability in oper...Aircraft assembly is characterized by stringent precedence constraints,limited resource availability,spatial restrictions,and a high degree of manual intervention.These factors lead to considerable variability in operator workloads and significantly increase the complexity of scheduling.To address this challenge,this study investigates the Aircraft Pulsating Assembly Line Scheduling Problem(APALSP)under skilled operator allocation,with the objective of minimizing assembly completion time.A mathematical model considering skilled operator allocation is developed,and a Q-Learning improved Particle Swarm Optimization algorithm(QLPSO)is proposed.In the algorithm design,a reverse scheduling strategy is adopted to effectively manage large-scale precedence constraints.Moreover,a reverse sequence encoding method is introduced to generate operation sequences,while a time decoding mechanism is employed to determine completion times.The problem is further reformulated as a Markov Decision Process(MDP)with explicitly defined state and action spaces.Within QLPSO,the Q-learning mechanism adaptively adjusts inertia weights and learning factors,thereby achieving a balance between exploration capability and convergence performance.To validate the effectiveness of the proposed approach,extensive computational experiments are conducted on benchmark instances of different scales,including small,medium,large,and ultra-large cases.The results demonstrate that QLPSO consistently delivers stable and high-quality solutions across all scenarios.In ultra-large-scale instances,it improves the best solution by 25.2%compared with the Genetic Algorithm(GA)and enhances the average solution by 16.9%over the Q-learning algorithm,showing clear advantages over the comparative methods.These findings not only confirm the effectiveness of the proposed algorithm but also provide valuable theoretical references and practical guidance for the intelligent scheduling optimization of aircraft pulsating assembly lines.展开更多
Dear Editor,Linear and whorled nevoid hypermelanosis(LWNH)is a rare,sporadic pigmentary disorder characterized by hyperpigmented macules arranged in linear streaks and whorls along Blaschko's lines,typically appea...Dear Editor,Linear and whorled nevoid hypermelanosis(LWNH)is a rare,sporadic pigmentary disorder characterized by hyperpigmented macules arranged in linear streaks and whorls along Blaschko's lines,typically appearing within the first few weeks of life[1],and remains a challenge to treat.Here,we report a case of LWNH and review the relevant literature to help clinicians better understand this disease.展开更多
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t...Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities.展开更多
To locate and quantify local damage in a simply supported bridge, in this study, we derived a rotational-angle influence line equation of a simply supported beam model with local damage. Using the diagram multiplicati...To locate and quantify local damage in a simply supported bridge, in this study, we derived a rotational-angle influence line equation of a simply supported beam model with local damage. Using the diagram multiplication method, we introduce an analytical formula for a novel damage-identification indicator, namely the diff erence of rotational-angle influence linescurvature(DRAIL-C). If the initial stiff ness of the simply supported beam is known, the analytical formula can be effectively used to determine the extent of damage under certain circumstances. We determined the effectiveness and anti-noise performance of this new damage-identification method using numerical examples of a simply supported beam, a simply supported hollow-slab bridge, and a simply supported truss bridge. The results show that the DRAIL-C is directly proportional to the moving concentrated load and inversely proportional to the distance between the bridge support and the concentrated load and the distance between the damaged truss girder and the angle measuring points. The DRAIL-C indicator is more sensitive to the damage in a steel-truss-bridge bottom chord than it is to the other elements.展开更多
Close-distance coal seams are widely distributed over China,and the coal pillars left by the overlying coal seams afect the retracement channel of the underlying coal seam in the stopping stage.Based on the engineerin...Close-distance coal seams are widely distributed over China,and the coal pillars left by the overlying coal seams afect the retracement channel of the underlying coal seam in the stopping stage.Based on the engineering background of close-distance seam mining in a coal mine,the reasonable position of the underlying coal seam's stopping line and the support method of the large section roadway during stopping are investigated using feld measurements,similar simulation experiments,and numerical simulations.There are three types of location relationships between the stopping line of the underlying coal seam and the stopping line of the overlying coal seam:"externally staggered with the upper stopping line"(ESUL,stops mining under the overlying goaf),"overlapped with upper stopping line"(OUL),and"internally staggered with the upper stopping line"(ISUL,ISUL-SD for shorter internal staggered distances,ISUL-LD for longer ones).There are diferent stress arch structures in the overlying strata of the above three positions,and the stress arch evolution process exists in the process of ESUL→OUL→ISUL-SD→ISUL-LD:a front and rear double stress arch structure→the front arch gradually decreases→the front arch dies out,and the double arch synthesizes the single arch→the single-arch range expands→the nested double arch.The relationship between the stress arch structure and the position of the stopping line is evaluated as follows:(1)ESUL:the stress concentration in the roof plate of the retracement channel of the underlying coal seam is the highest,because the overburden block of the extensive collapse zone acts directly on the roof plate of the retracement channel,resulting in relative difculties in roof support.(2)OUL:although the retracement channel roof pressure is minimal,the overlying rock structure has the potential for rotation or slippage instability.(3)ISUL-SD:the pressure on the roof of the retracement channel is small and the overburden structure is stable,which is conducive to the safe retraction of the support and not limited by the width of the end-mining coal pillar.(4)ISUL-LD:it is basically the same as the condition of stopping under the non-goaf;however,it has a limitation on the width of the end-mining coal pillar.The location of the stopping line is selected as ISUL-SD,and the retraction process of the self-excavating retraction channel was adopted.A partition asymmetric support scheme which is proven by feld practice is proposed,through a comprehensive analysis of the pre-stress feld simulation of the support scheme,based on the diferent control requirements of the roof above the support and the roof of the retracement channel in the stopping area.This method realizes safe and smooth withdrawal of the support.展开更多
BACKGROUND Colon cancer cell lines are widely used for research and for the screening of drugs that specifically target the stem cell compartment of colon cancers.It was reported that colon cancer carcinoma specimens ...BACKGROUND Colon cancer cell lines are widely used for research and for the screening of drugs that specifically target the stem cell compartment of colon cancers.It was reported that colon cancer carcinoma specimens contain a subset of leucine-rich repeatcontaining G protein-coupled receptor 5(LGR5)-expressing stem cells,these socalled“tumour-initiating”cells,reminiscent in their properties of the normal intestinal stem cells(ISCs),may explain the apparent heterogeneity of colon cancer cell lines.Also,colon cancer is initiated by aberrant Wnt signaling in ISCs known to express high levels of LGR5.Furthermore,in vivo reports demonstrate the clonal expansion of intestinal adenomas from a single LGR5-expressing cell.AIM To investigate whether colon cancer cell lines contain cancer stem cells and to characterize these putative cancer stem cells.METHODS A portable fluorescent reporter construct based on a conserved fragment of the LGR5 promoter was used to isolate the cell compartments expressing different levels of LGR5 in two widely used colon cancer cell lines(Caco-2 and LoVo).These cells were then characterized according to their proliferation capacity,gene expression signatures of ISC markers,and their tumorigenic properties in vivo and in vitro.RESULTS The data revealed that the LGR5 reporter can be used to identify and isolate a classical intestinal crypt stem cell-like population from the Caco-2,but not from the LoVo,cell lines,in which the cancer stem cell population is more akin to B lymphoma Moloney murine leukemia virus insertion region 1 homolog(+4 crypt)stem cells.This sub-population within Caco-2 cells exhibits an intestinal cancer stem cell gene expression signature and can both self-renew and generate differentiated LGR5 negative progeny.Our data also show that cells expressing high levels of LGR5/enhanced yellow fluorescent protein(EYFP)from this cell line exhibit tumorigenic-like properties in vivo and in vitro.In contrast,cell compartments of LoVo that are expressing high levels of LGR5/EYFP did not show these stem cell-like properties.Thus,cells that exhibit high levels of LGR5/EYFP expression represent the cancer stem cell compartment of Caco-2 colon cancer cells,but not LoVo cells.CONCLUSION Our findings highlight the presence of a spectrum of different ISC-like compartments in different colon cancer cell lines.Their existence is an important consideration for their screening applications and should be taken into account when interpreting drug screening data.We have generated a portable LGR5-reporter that serves as a valuable tool for the identification and isolation of different colon cancer stem cell populations in colon cancer lines.展开更多
This paper reports a novel dual-band coupled-line bandpass filter.Comprising a quadruple-mode coupled-line resonator,this proposed filter could filter signals on two frequency bands.After introducing two varactors,one...This paper reports a novel dual-band coupled-line bandpass filter.Comprising a quadruple-mode coupled-line resonator,this proposed filter could filter signals on two frequency bands.After introducing two varactors,one of the four resonances could be continuously altered by changing the capacitance of the varactors,thus the two frequency bandwidths could be independently tunned.In this paper,the detailed explanation for its operation is given,and the agreement between the expected and practical performance sufficiently confirms the robustness and effectiveness of this proposed filter.展开更多
Introgression lines （ILs） are valuable materials for identifying quantitative trait loci （QTLs）, evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs （BC5F3） containing segments from u...Introgression lines （ILs） are valuable materials for identifying quantitative trait loci （QTLs）, evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs （BC5F3） containing segments from upland tropical japonica cultivar IRAT109 in a lowland temperate japonica cultivar Yuefu background were developed. One hundred and seventy-six polymorphic markers were used to identify introgressed segments. No segment from IRAT109 was found in 160 lines. Introgressed segments of the other 270 lines covered 99.1% of the donor genome. The mean number of introgressed donor segments per individual was 3.3 with an average length of 14.4 cM. QTL analysis was conducted on basal root thickness （BRT） of the 270 ILs grown under irrigated lowland, upland and hydroponic conditions. A total of 22 QTLs affecting BRT were identified, six QTLs （qBRT3.1, qBRT3.2, qBRT6.1, qBRTS.2, qBRT9.1, and qBRT9.2） were consistently expressed under at least two environments （location and water regime）, and qBRT7.2 was a new BRT QTL identified under lowland conditions. IL255 containing qBRT9.1 showed an increase of 10.09% and 7.07% BRT over cultivar Yuefu when grown under upland and lowland conditions, respectively. Using a population of 304 F2：3 lines derived from the cross IL255 Yuefu, qBRT9.1 was validated and mapped to a 1.2 cM interval between RM24271 and RM566. The presence of qBRT9.1 explained 12% of BRT variation. The results provide upland rice ILs and BRT QTLs for analyzing the genetic basis of drought resistance, detecting favorable genes from upland rice, and rice drought resistance breeding.展开更多
Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta(E.hirta)in MCF-7 cell line model using comet assay.Methods:The cytotoxicity of E.hirta extract was investigated by employing brine sh...Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta(E.hirta)in MCF-7 cell line model using comet assay.Methods:The cytotoxicity of E.hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E.hirta was assessed by using Comet assay.Results:Both toxicity tests exhibited significant toxicity result.In the comet assay,the E.hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage.The extract of E.hirta showed significant toxicity against brine shrimp with an LC_(50)value of 620.382μg/mL(24 h).Comparison with positive control potassium dichroniate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity.Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E.hirta.However,the observed toxicity of E.hta extracts needs to be confirmed in additional studies.展开更多
Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy （SBCE） sys- te...Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy （SBCE） sys- tems （PillCam and MiroCam） was performed. The oral cavity/aero-digestive tract （i.e., tongue, uvula and/or epiglottis） was captured/identified in almost all （99%） of PillCam videos but in none of MiroCam cases, P 〈 0.0001. Furthermore, oesophageal images （i.e., from the upper oesophageal sphincter to the Z-line were cap- tured in 99% of PillCam videos （mean =1= SD, 60.5 ± 334.1 frames, range： 0-3329 frames） and in 66% of Mi- roCam cases （mean ± SD, 11.1 ± 46.5 frames, range： 0-382 frames）, P 〈 0.0001. The Z-line was identified in 42% of PilICam videos and 17% of MiroCam, P = 0.0002. This information might be useful when perform- ing SBCE in patients with high risks for aspiration.展开更多
Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A （A...Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A （ALV-A） env gene into plasmid pcDNA3.1/Zeo （＋） and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCIDso/0.1 mL （50% tissue culture infective dose）ALV-A and was partially resistant to 10~ TCIDs0/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISAfor capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool.展开更多
文摘A new sterile line UP-3s, which carries the Dominant Early Maturity Gene (DEMG), was bred on the farm of University of Arkansas at Pine Bluff (UAPB). UP-3s and two check sterile lines, Jin23-A and Xie-A which do not carry the Dominant Early Maturity Gene, were crossed with a group of different maturity restorer lines, PB-1R, PB-5R,PB11, PB-13R, PB-20, PB-21, PB-22R, and PB-23R. Eighteen new hybrid rice combinations of these crosses were then tested at UAPB in 2012 and 2013. The results showed that panicle differentiation (PD) of hybrids from female parent UP-3s (DEMG) crossed with the 8 male parents, were earlier than the hybrids from female parent Jin23-A or Xie-A crossed with the 8 male parents. The PD of these earlier hybrids was before Jun 25 and heading was before July 20. Early PD and heading avoided the high temperature (over 34°C) period which usually occurs after July 20 in Arkansas. The yields of these earlier maturity hybrids with female parent UP-3s were higher than those of the late maturity hybrids thatwereF1 progeny of sterile lines Jin23-A or Xie-A (these two female parent checks with non-DEMG). These results showed that the DEMG sterile line UP-3s can be adopted in making crosses with later maturity restorer lines to obtain earlier maturity hybrids to avoid the high temperature period in Arkansas.
文摘The voltage uprating of existing overhead lines is an interesting solution for increasing the transmission of electricity, especially in areas where it is difficult to build new lines. If a line is uprated with minor changes of its design and without improvement of the grounding electrodes of towers, its lightning performance remains unchanged. The consequence is that the uprated line will have a lightning flashover rate very high compared to an overhead line of same nominal voltage but of standard design. One attractive solution to solve this problem is to use line arresters. The goal of this paper is to study the use of line arresters to achieve a satisfactory lightning performance of an overhead line uprated from 225 kV to 400 kV without major design modifications. In order to compare different strategies of line arrester installation the flashover rate is calculated based on a software able to launch automatically EMTP-RV. The software named LIPS has been developed under the cover of a partnership between Hydro-Quebec, RTE and EDF.
基金supported by the National Key Research and Development Program of China(Grant No.2019YFE0118400).
文摘Random integration of massive distributed photovoltaic(PV)generation poses serious challenges to distribution networks.Voltage violations,line overloads,increased peak-valley differences,and power-flow reversals can occur at different locations,times,and severities.Traditional planning methods such as energy storage(ES)allocation and upgrading of lines may result in poor economics and low equipment utilization.This study proposes a distribution-network planning strategy that coordinates three planning mechanisms:ES allocation to substations and to feeders,and line upgrading.The proposed strategy achieved the lowest annual cost,considering the distribution network problems at different locations,times,and severities.A net load curve description method was first proposed considering economic planning and the uncertain distribution of loads and PVs.Second,a multistage optimization model for ES allocation and line upgrading was established based on the net load curves.Finally,an improved IEEE33 node system was used in the case study.The planning results show that the proposed coordinated planning strategy can address the problems caused by the random integration of massive distributed PVs,improve equipment utilization,and obtain the lowest total annual planning cost.
基金the National Natural Science Foundation of China(Nos.32473189,32173001,32273115)the Guangdong Province Special Support Plan Youth Top Talent Project(No.NIQN2024002)+2 种基金the Scientific and Technological Planning Project of Guangzhou City(No.2023B03J1267)the Natural Science Foundation of Guangxi(No.2021GXNSFDA075015)the Natural Science Foundation of Guangdong Province(Nos.2023B1515120074,2024A1515010880)。
文摘Leopard coral grouper(Plectropomus leopardus)is a commercially important marine fish species.It is important to study how to prevent it from infecting with various viruses.In this study,we established and characterized a new cell line derived from the fin tissue of leopard coral grouper(PLF).The PLF cells were cultured for more than 55 passages.Cytochrome B gene sequencing confirmed the origin of the PLF cells is P.leopardus.Immunostaining against cytokeratin indicated that the PLF cells predominantly consist of epithelial cells.The chromosome number of PLF was 48.The cells grew well in Dulbecco's modified Eagle's medium(DMEM)supplemented with 10%–20%fetal bovine serum(FBS)at temperature between 20–28℃,with the highest growth rate at28℃.Transfection with pEGFP-N3 plasmid showed the transfection efficiency was about 35%.Virus susceptibility tests revealed that PLF cells are susceptible to red-spotted grouper nervous necrosis virus(NNV)and viral hemorrhagic septicemia virus(VHSV),and viral proliferation was confirmed by qRT-PCR and western blot.The altered expressions of immune-related genes TBK1,IRF3,and Mx after NNV and VHSV infections suggested that PLF cells can mount an immune response to fish viruses.Thus the PLF cells can be employed for studying virus-host interactions and developing antiviral strategies.
文摘The published article titled“Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24”has been retracted from Oncology Research,Vol.26,No.8,2018,pp.1227–1234.
文摘The published article titled“CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line”has been retracted from Oncology Research,Vol.24,No.6,2016,pp.487–494.
基金supported by the National Natural Science Foundation of China(Grant No.51507106)China Postdoctoral Science Foundation(Grant No.2021M702371)。
文摘To ensure the safety of power energy transmission channel and mitigate the harm caused by galloping of iced transmission lines,the axial time-delay velocity feedback strategy is adopted to suppress the galloping.The par-tial differential equation of galloping with axial time-delay velocity feedback strategy is established based on the variational principle for Hamiltonian.Then,the partial differential equation of galloping is transformed into or-dinary differential equation based on normalization and the Galerkin method.The primary amplitude-frequency response equation,the first-order steady-state approximate solution,and the harmonic amplitude-frequency re-sponse equation are derived by the multiscale method.The impact of different parameters such as time-delay value,control coefficient,and amplitude of external excitation on the galloping response are analyzed.The am-plitude under the primary resonance exhibits periodicity as time-delay value varies.The amplitude diminishes with increased control coefficient and increases with external excitation.Comprehensive consideration of vari-ous influences of parameters on vibration characteristics is crucial when employing the axial time-delay velocity feedback strategy to suppress galloping.Therefore,to achieve the best vibration suppression effect,it is crucial to adjust the time-delay parameter for modifying the range and amplitude of the resonance zone.The conclusions obtained by this study are expected to advance the refinement of active control techniques for iced transmission lines,and may provide valuable insights for practical engineering applications.
文摘China International Supply Chain Expo(hereinafter referred to as the CISCE)is a key platform for enterprises to showcase their innovation strength and connect with global resources.As a“hidden champion”in the field of intelligent manufacturing,Hunan SpeedBot Robot ics Co.,Ltd.(hereinaf ter refer red to as“SpeedBot”)is planning to display its independently developed“AI+3D Vision”core technology at the CISCE,to empower the supply chain of industries such as automobiles and heavy industry through a full-process automated detection solution.Liu Tingting,Vice General Manager of SpeedBot,said in an interview with China’s Foreign Trade,“The CISCE is not only a window for technology display,but also a link to promote collaborative innovations of the whole industrial chain.We look forward to building resilient supply chains with global partners and bringing China’s‘smart’manufacturing to the world.”
文摘Over the next 20 years,China's urban rail transit(hereinafter referred to as'urban rail')will face large-scalerenovation of existing line facilities and equipment,with more than 1000 km of renovated lines to be added eachyear.In 2024,the China Association of Metros issued the Guiding Opinions on the Renovation of Existing UrbanRail Transit Lines in China,providing guiding opinions on norms,standards,and implementation approaches forthe renovation of existing lines in the coming period.In the practical work of renovating existing urban rail lines,it is necessary to continuously explore and refine relevant theoretical methods in line with industry developmenttrends and urban development requirements.The following are the author's recent reflections on theoreticalinnovation in this field.
基金supported by the National Natural Science Foundation of China(Grant No.52475543)Natural Science Foundation of Henan(Grant No.252300421101)+1 种基金Henan Province University Science and Technology Innovation Talent Support Plan(Grant No.24HASTIT048)Science and Technology Innovation Team Project of Zhengzhou University of Light Industry(Grant No.23XNKJTD0101).
文摘Aircraft assembly is characterized by stringent precedence constraints,limited resource availability,spatial restrictions,and a high degree of manual intervention.These factors lead to considerable variability in operator workloads and significantly increase the complexity of scheduling.To address this challenge,this study investigates the Aircraft Pulsating Assembly Line Scheduling Problem(APALSP)under skilled operator allocation,with the objective of minimizing assembly completion time.A mathematical model considering skilled operator allocation is developed,and a Q-Learning improved Particle Swarm Optimization algorithm(QLPSO)is proposed.In the algorithm design,a reverse scheduling strategy is adopted to effectively manage large-scale precedence constraints.Moreover,a reverse sequence encoding method is introduced to generate operation sequences,while a time decoding mechanism is employed to determine completion times.The problem is further reformulated as a Markov Decision Process(MDP)with explicitly defined state and action spaces.Within QLPSO,the Q-learning mechanism adaptively adjusts inertia weights and learning factors,thereby achieving a balance between exploration capability and convergence performance.To validate the effectiveness of the proposed approach,extensive computational experiments are conducted on benchmark instances of different scales,including small,medium,large,and ultra-large cases.The results demonstrate that QLPSO consistently delivers stable and high-quality solutions across all scenarios.In ultra-large-scale instances,it improves the best solution by 25.2%compared with the Genetic Algorithm(GA)and enhances the average solution by 16.9%over the Q-learning algorithm,showing clear advantages over the comparative methods.These findings not only confirm the effectiveness of the proposed algorithm but also provide valuable theoretical references and practical guidance for the intelligent scheduling optimization of aircraft pulsating assembly lines.
文摘Dear Editor,Linear and whorled nevoid hypermelanosis(LWNH)is a rare,sporadic pigmentary disorder characterized by hyperpigmented macules arranged in linear streaks and whorls along Blaschko's lines,typically appearing within the first few weeks of life[1],and remains a challenge to treat.Here,we report a case of LWNH and review the relevant literature to help clinicians better understand this disease.
基金National Science and Technology Council,the Republic of China,under grants NSTC 113-2221-E-194-011-MY3 and Research Center on Artificial Intelligence and Sustainability,National Chung Cheng University under the research project grant titled“Generative Digital Twin System Design for Sustainable Smart City Development in Taiwan.
文摘Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities.
基金supported by the National Natural Science Foundation of China(Nos.51608245 and 51568041)Natural Science Foundation of Gansu Province(Nos.148RJZA026 and 2014GS02269)
文摘To locate and quantify local damage in a simply supported bridge, in this study, we derived a rotational-angle influence line equation of a simply supported beam model with local damage. Using the diagram multiplication method, we introduce an analytical formula for a novel damage-identification indicator, namely the diff erence of rotational-angle influence linescurvature(DRAIL-C). If the initial stiff ness of the simply supported beam is known, the analytical formula can be effectively used to determine the extent of damage under certain circumstances. We determined the effectiveness and anti-noise performance of this new damage-identification method using numerical examples of a simply supported beam, a simply supported hollow-slab bridge, and a simply supported truss bridge. The results show that the DRAIL-C is directly proportional to the moving concentrated load and inversely proportional to the distance between the bridge support and the concentrated load and the distance between the damaged truss girder and the angle measuring points. The DRAIL-C indicator is more sensitive to the damage in a steel-truss-bridge bottom chord than it is to the other elements.
基金supported by the National Natural Science Foundation of China(52004286)the Fundamental Research Funds for the Central Universities(2022XJNY02)+3 种基金the National Natural Science Foundation of China(51974317,52074296)the China Postdoctoral Science Foundation(2020T130701,2019M650895)the Fundamental Research Funds for the Central Universities(2022YJSNY18,2022YJSNY09)all of which were gratefully acknowledged.
文摘Close-distance coal seams are widely distributed over China,and the coal pillars left by the overlying coal seams afect the retracement channel of the underlying coal seam in the stopping stage.Based on the engineering background of close-distance seam mining in a coal mine,the reasonable position of the underlying coal seam's stopping line and the support method of the large section roadway during stopping are investigated using feld measurements,similar simulation experiments,and numerical simulations.There are three types of location relationships between the stopping line of the underlying coal seam and the stopping line of the overlying coal seam:"externally staggered with the upper stopping line"(ESUL,stops mining under the overlying goaf),"overlapped with upper stopping line"(OUL),and"internally staggered with the upper stopping line"(ISUL,ISUL-SD for shorter internal staggered distances,ISUL-LD for longer ones).There are diferent stress arch structures in the overlying strata of the above three positions,and the stress arch evolution process exists in the process of ESUL→OUL→ISUL-SD→ISUL-LD:a front and rear double stress arch structure→the front arch gradually decreases→the front arch dies out,and the double arch synthesizes the single arch→the single-arch range expands→the nested double arch.The relationship between the stress arch structure and the position of the stopping line is evaluated as follows:(1)ESUL:the stress concentration in the roof plate of the retracement channel of the underlying coal seam is the highest,because the overburden block of the extensive collapse zone acts directly on the roof plate of the retracement channel,resulting in relative difculties in roof support.(2)OUL:although the retracement channel roof pressure is minimal,the overlying rock structure has the potential for rotation or slippage instability.(3)ISUL-SD:the pressure on the roof of the retracement channel is small and the overburden structure is stable,which is conducive to the safe retraction of the support and not limited by the width of the end-mining coal pillar.(4)ISUL-LD:it is basically the same as the condition of stopping under the non-goaf;however,it has a limitation on the width of the end-mining coal pillar.The location of the stopping line is selected as ISUL-SD,and the retraction process of the self-excavating retraction channel was adopted.A partition asymmetric support scheme which is proven by feld practice is proposed,through a comprehensive analysis of the pre-stress feld simulation of the support scheme,based on the diferent control requirements of the roof above the support and the roof of the retracement channel in the stopping area.This method realizes safe and smooth withdrawal of the support.
基金We thank Professor McGuckin M(MMRI,Brisbane)for providing human colon cancer cell lines(Caco-2,LoVo,and SW480)Dr.Rolfe B(AIBN,Brisbane)for providing mouse NSC-34 cells.
文摘BACKGROUND Colon cancer cell lines are widely used for research and for the screening of drugs that specifically target the stem cell compartment of colon cancers.It was reported that colon cancer carcinoma specimens contain a subset of leucine-rich repeatcontaining G protein-coupled receptor 5(LGR5)-expressing stem cells,these socalled“tumour-initiating”cells,reminiscent in their properties of the normal intestinal stem cells(ISCs),may explain the apparent heterogeneity of colon cancer cell lines.Also,colon cancer is initiated by aberrant Wnt signaling in ISCs known to express high levels of LGR5.Furthermore,in vivo reports demonstrate the clonal expansion of intestinal adenomas from a single LGR5-expressing cell.AIM To investigate whether colon cancer cell lines contain cancer stem cells and to characterize these putative cancer stem cells.METHODS A portable fluorescent reporter construct based on a conserved fragment of the LGR5 promoter was used to isolate the cell compartments expressing different levels of LGR5 in two widely used colon cancer cell lines(Caco-2 and LoVo).These cells were then characterized according to their proliferation capacity,gene expression signatures of ISC markers,and their tumorigenic properties in vivo and in vitro.RESULTS The data revealed that the LGR5 reporter can be used to identify and isolate a classical intestinal crypt stem cell-like population from the Caco-2,but not from the LoVo,cell lines,in which the cancer stem cell population is more akin to B lymphoma Moloney murine leukemia virus insertion region 1 homolog(+4 crypt)stem cells.This sub-population within Caco-2 cells exhibits an intestinal cancer stem cell gene expression signature and can both self-renew and generate differentiated LGR5 negative progeny.Our data also show that cells expressing high levels of LGR5/enhanced yellow fluorescent protein(EYFP)from this cell line exhibit tumorigenic-like properties in vivo and in vitro.In contrast,cell compartments of LoVo that are expressing high levels of LGR5/EYFP did not show these stem cell-like properties.Thus,cells that exhibit high levels of LGR5/EYFP expression represent the cancer stem cell compartment of Caco-2 colon cancer cells,but not LoVo cells.CONCLUSION Our findings highlight the presence of a spectrum of different ISC-like compartments in different colon cancer cell lines.Their existence is an important consideration for their screening applications and should be taken into account when interpreting drug screening data.We have generated a portable LGR5-reporter that serves as a valuable tool for the identification and isolation of different colon cancer stem cell populations in colon cancer lines.
基金supported by National Basic Research Program of China(973 Program)(No.2014CB339900)National Natural Science Foundations of China(No.61422103,and 61327806)
文摘This paper reports a novel dual-band coupled-line bandpass filter.Comprising a quadruple-mode coupled-line resonator,this proposed filter could filter signals on two frequency bands.After introducing two varactors,one of the four resonances could be continuously altered by changing the capacitance of the varactors,thus the two frequency bandwidths could be independently tunned.In this paper,the detailed explanation for its operation is given,and the agreement between the expected and practical performance sufficiently confirms the robustness and effectiveness of this proposed filter.
基金funded by the National Basic Research Program of China(No.2010CB1125904)the National Key Technologies R&D Program of China(No.2009BADA2B01)+2 种基金the National Natural Science Foundation of China(No. 31061140458)"948"Project of MOA(No.2011-G2B)the Key Project of Transgenic Crop Improvement of China (No.2009ZX08009-073B)
文摘Introgression lines （ILs） are valuable materials for identifying quantitative trait loci （QTLs）, evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs （BC5F3） containing segments from upland tropical japonica cultivar IRAT109 in a lowland temperate japonica cultivar Yuefu background were developed. One hundred and seventy-six polymorphic markers were used to identify introgressed segments. No segment from IRAT109 was found in 160 lines. Introgressed segments of the other 270 lines covered 99.1% of the donor genome. The mean number of introgressed donor segments per individual was 3.3 with an average length of 14.4 cM. QTL analysis was conducted on basal root thickness （BRT） of the 270 ILs grown under irrigated lowland, upland and hydroponic conditions. A total of 22 QTLs affecting BRT were identified, six QTLs （qBRT3.1, qBRT3.2, qBRT6.1, qBRTS.2, qBRT9.1, and qBRT9.2） were consistently expressed under at least two environments （location and water regime）, and qBRT7.2 was a new BRT QTL identified under lowland conditions. IL255 containing qBRT9.1 showed an increase of 10.09% and 7.07% BRT over cultivar Yuefu when grown under upland and lowland conditions, respectively. Using a population of 304 F2：3 lines derived from the cross IL255 Yuefu, qBRT9.1 was validated and mapped to a 1.2 cM interval between RM24271 and RM566. The presence of qBRT9.1 explained 12% of BRT variation. The results provide upland rice ILs and BRT QTLs for analyzing the genetic basis of drought resistance, detecting favorable genes from upland rice, and rice drought resistance breeding.
基金supported by USM Short Term Grant(304/CIPPM/6312034)from Universiti Sains Malaysia
文摘Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta(E.hirta)in MCF-7 cell line model using comet assay.Methods:The cytotoxicity of E.hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E.hirta was assessed by using Comet assay.Results:Both toxicity tests exhibited significant toxicity result.In the comet assay,the E.hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage.The extract of E.hirta showed significant toxicity against brine shrimp with an LC_(50)value of 620.382μg/mL(24 h).Comparison with positive control potassium dichroniate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity.Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E.hirta.However,the observed toxicity of E.hta extracts needs to be confirmed in additional studies.
文摘Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy （SBCE） sys- tems （PillCam and MiroCam） was performed. The oral cavity/aero-digestive tract （i.e., tongue, uvula and/or epiglottis） was captured/identified in almost all （99%） of PillCam videos but in none of MiroCam cases, P 〈 0.0001. Furthermore, oesophageal images （i.e., from the upper oesophageal sphincter to the Z-line were cap- tured in 99% of PillCam videos （mean =1= SD, 60.5 ± 334.1 frames, range： 0-3329 frames） and in 66% of Mi- roCam cases （mean ± SD, 11.1 ± 46.5 frames, range： 0-382 frames）, P 〈 0.0001. The Z-line was identified in 42% of PilICam videos and 17% of MiroCam, P = 0.0002. This information might be useful when perform- ing SBCE in patients with high risks for aspiration.
基金The work was founded by the National Key R&D Program of China(2016YFD0501606)the Public Industry Research Program,the Ministry of Agriculture of China(201203055)+2 种基金the Program of Science and Technology Development of Guangdong Province,China(2015A020209145)the China Meat-Type Chicken Research System(CARS-42-G09)the Modern Agriculture Talents Support Program,Ministry of Agriculture of China([2012] no.160)
文摘Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A （ALV-A） env gene into plasmid pcDNA3.1/Zeo （＋） and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCIDso/0.1 mL （50% tissue culture infective dose）ALV-A and was partially resistant to 10~ TCIDs0/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISAfor capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool.
