[Objective] The aim was to study the germination behavior of Tamarix elongata Ledeb.under natural conditions.[Method] By using T.elongata as the experimental materials,the seed morphology and germination characteristi...[Objective] The aim was to study the germination behavior of Tamarix elongata Ledeb.under natural conditions.[Method] By using T.elongata as the experimental materials,the seed morphology and germination characteristics were observed and measured.[Result] ① T.elongata seed was 1 011.96 μm in length and 399.94 μm in width,and the thousand-seed weight was 38.66 mg;② the germination rate,germination energy,germination index and germination coefficient of T.elongata seeds were 95.56%,46.67%,20.25 and 56.86 respectively;③ there were no significant differences in morphology and germination characteristics among the three different geographical populations.[Conclusion] The present research will provide reference for the introduction and breeding of T.elongata as well as the protection of sand dune vegetation.展开更多
A simple and accurate high-performance liquid chromatography method （HPLC） was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of th...A simple and accurate high-performance liquid chromatography method （HPLC） was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable ehro- matographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid （H3PO4） as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality ofM. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.展开更多
To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effect...To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.展开更多
A new flavone glucoside,selagin-7-O-（6″-O-acetyl-）-β-D-glycoside,has been isolated from Cancrinia discoidea（Ledeb.） Poljak.Its structure was identified by spectroscopic methods,2D NMR and MS.Compounds exhibited ...A new flavone glucoside,selagin-7-O-（6″-O-acetyl-）-β-D-glycoside,has been isolated from Cancrinia discoidea（Ledeb.） Poljak.Its structure was identified by spectroscopic methods,2D NMR and MS.Compounds exhibited activity of anti-inflammatory in vitro.展开更多
[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was us...[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.展开更多
Background Melilotus suaveolens Ledeb （M. suaveolens Ledeb） has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant. Me...Background Melilotus suaveolens Ledeb （M. suaveolens Ledeb） has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant. Methods Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography （HPLC）. While dexamethasone （DM） was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor α （TNF-α） mRNA, cyclooxygenase 2 （COX-2） mRNA, COX-2 and nuclear factor KB （NF-KB） of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Westem blot analysis and immunocytochemical assay, respectively. Results Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-α mRNA, COX-2 mRNA and NF-KB to some extent. Conclusions Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.展开更多
Reconstructing the hydrological change based on dendrohydrological data has important implications for understanding the dynamic distribution and evolution pattern of a given river. The widespread, long-living conifer...Reconstructing the hydrological change based on dendrohydrological data has important implications for understanding the dynamic distribution and evolution pattern of a given river. The widespread, long-living coniferous forests on the Altay Mountains provide a good example for carrying out the dendrohydrological studies. In this study, a regional composite tree-ring width chronology developed by Lariat sibirica Ledeb. and Picea obovata Ledeb. was used to reconstruct a 301-year annual （from preceding July to succeeding June） streamflow for the Haba River, which originates in the southern Altay Mountains, Xinjiang, China. Results indicated that the reconstructed streamflow series and the observations were fitting well, and explained 47.5% of the variation in the observed streamflow of 1957-2008. Moreover, floods and droughts in 1949-2000 were precisely captured by the streamflow reconstruction. Based on the frequencies of the wettest/driest years and decades, we identified the 19th century as the century with the largest occurrence of hydrological fluctuations for the last 300 years. After applying a 21-year moving average, we found five wet （1724-1758, 1780-1810, 1822-1853, 1931-1967, and 1986-2004） and four dry （1759-1779, 1811-1821, 1854-1930, and 1968-1985） periods in the streamflow reconstruction. Furthermore, four periods （1770-1796, 1816-1836, 1884-1949, and 1973-1997） identified by the streamflow series had an obvious increasing trend. The increasing trend of streamflow since the 1970s was the biggest in the last 300 years and coincided with the recent warming-wetting trend in northwestern China. A significant correlation between streamflow and precipitation in the Altay Mountains indicated that the streamflow reconstruction contained not only local, but also broad-scale, hydro-climatic signals. The 24-year, 12-year, and 2.2-4.5-year cycles of the reconstruction revealed that the streamflow variability of the Haba River may be influenced by solar activity and the atmosphere-ocean system.展开更多
基金Supported by a Grant from the National Natural Science Foundation of China(30670133)~~
文摘[Objective] The aim was to study the germination behavior of Tamarix elongata Ledeb.under natural conditions.[Method] By using T.elongata as the experimental materials,the seed morphology and germination characteristics were observed and measured.[Result] ① T.elongata seed was 1 011.96 μm in length and 399.94 μm in width,and the thousand-seed weight was 38.66 mg;② the germination rate,germination energy,germination index and germination coefficient of T.elongata seeds were 95.56%,46.67%,20.25 and 56.86 respectively;③ there were no significant differences in morphology and germination characteristics among the three different geographical populations.[Conclusion] The present research will provide reference for the introduction and breeding of T.elongata as well as the protection of sand dune vegetation.
基金supported by Hubei and Wuhan Engineering Technology Center of Modernization Traditional Chinese Medicine Wuhan Jianmin Pharmacy of TCM Co. Ltd,Wuhan 430054,China.
文摘A simple and accurate high-performance liquid chromatography method （HPLC） was described to generate the metabolite fingerprints of Melilotus. suaveolens Ledeb of different harvesting times and different parts of the plant. A suitable ehro- matographic system was established using a linear gradient elution with acetonitrile and 0.1% phosphoric acid （H3PO4） as the mobile phase and a detection wavelength of 210 nm. HPLC analysis identified 12 common peaks and 8 common fingerprint peaks among the different parts of the herb. We found that the constituents varied at different harvesting times and in different parts of the plant. The HPLC fingerprint method could be used to evaluate the quality ofM. suaveolens Ledeb and Melilotus genus herbs. The same strategy can also be applied for the development of fingerprints for the quality control of other herbal medicines.
基金supported by Research Foundation for Science and Technology Innovation Talents of Harbin(2010RFQXS072)Scientific Research Foundation for the Returned Overseas Chinese Scholars of Heilongjiang Province of China(200923)
文摘To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.
基金supported by the National Natural Science Foundation of China(No.20676041)by the China Postdoctoral Science Foundation(No.2004036506)
文摘A new flavone glucoside,selagin-7-O-（6″-O-acetyl-）-β-D-glycoside,has been isolated from Cancrinia discoidea（Ledeb.） Poljak.Its structure was identified by spectroscopic methods,2D NMR and MS.Compounds exhibited activity of anti-inflammatory in vitro.
文摘[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.
文摘Background Melilotus suaveolens Ledeb （M. suaveolens Ledeb） has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant. Methods Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography （HPLC）. While dexamethasone （DM） was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor α （TNF-α） mRNA, cyclooxygenase 2 （COX-2） mRNA, COX-2 and nuclear factor KB （NF-KB） of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Westem blot analysis and immunocytochemical assay, respectively. Results Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-α mRNA, COX-2 mRNA and NF-KB to some extent. Conclusions Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.
基金supported by the National Natural Science Foundation of China (41275120, 41605047)the Shanghai Cooperation Organization Science and Technology Partnership (2017E01032)+1 种基金the Special Foundation for Asian Regional Cooperation (Climate Reconstruction of Tian Shan in China, Kyrgyzstan and Tajikistan)the Autonomous Region Youth Science and Technology Innovation Talents Training Project (qn2015bs025)
文摘Reconstructing the hydrological change based on dendrohydrological data has important implications for understanding the dynamic distribution and evolution pattern of a given river. The widespread, long-living coniferous forests on the Altay Mountains provide a good example for carrying out the dendrohydrological studies. In this study, a regional composite tree-ring width chronology developed by Lariat sibirica Ledeb. and Picea obovata Ledeb. was used to reconstruct a 301-year annual （from preceding July to succeeding June） streamflow for the Haba River, which originates in the southern Altay Mountains, Xinjiang, China. Results indicated that the reconstructed streamflow series and the observations were fitting well, and explained 47.5% of the variation in the observed streamflow of 1957-2008. Moreover, floods and droughts in 1949-2000 were precisely captured by the streamflow reconstruction. Based on the frequencies of the wettest/driest years and decades, we identified the 19th century as the century with the largest occurrence of hydrological fluctuations for the last 300 years. After applying a 21-year moving average, we found five wet （1724-1758, 1780-1810, 1822-1853, 1931-1967, and 1986-2004） and four dry （1759-1779, 1811-1821, 1854-1930, and 1968-1985） periods in the streamflow reconstruction. Furthermore, four periods （1770-1796, 1816-1836, 1884-1949, and 1973-1997） identified by the streamflow series had an obvious increasing trend. The increasing trend of streamflow since the 1970s was the biggest in the last 300 years and coincided with the recent warming-wetting trend in northwestern China. A significant correlation between streamflow and precipitation in the Altay Mountains indicated that the streamflow reconstruction contained not only local, but also broad-scale, hydro-climatic signals. The 24-year, 12-year, and 2.2-4.5-year cycles of the reconstruction revealed that the streamflow variability of the Haba River may be influenced by solar activity and the atmosphere-ocean system.
