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In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea(Camellia sinensis L.) 被引量:2
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作者 Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第12期959-963,共5页
Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.... Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500,1.000 and 2.000 mg/mL) of black tea brew(BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate(EGCG) was used as the reference agent(concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL).Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid(from rooster comb)and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC50=(1.09±0.12) mg/mL]and dose dependent(r2=0.94) anti-hyaluronidase activity.EGCG also exhibited dose dependent(r2=0.93,P【0.05) anti-hyaluronidase activity which was superior[IC50=(0.09±0.00) mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation. 展开更多
关键词 Camellia sinensis Orange pekoe Black tea Sri lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid
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A Sri Lankan Development Communication Model: Use of Traditional Forms of Communication
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作者 Manoj Jinadasa Chandrasiri Rajapaksha Upali Pannilage 《Journalism and Mass Communication》 2016年第8期427-454,共28页
This paper explores the potentials of traditional forms of communication in rural development communication. Experimenting a local development communication model that can be used at the grass-root level communication... This paper explores the potentials of traditional forms of communication in rural development communication. Experimenting a local development communication model that can be used at the grass-root level communication at the community development programs in Sri Lanka is the research objective. This study is a participatory research. Live participatory observation, impact analysis using a questionnaire and direct interview tools were used to analyze the impact of the creative model. This research study was experimented at Rasnayakapura divisional secretariat, Sri Lanka from 2004 to 2005. Results suggest that the traditional forms of communication can be utilized as a useful instrument in the rural community programs more than the dominant use of mass media and conventional use of small group communication. Forms of traditional communication are rich in communication because of their closeness to rural life, credibility, Utilization of familiar signs & symbols, Participation of the same community, collective presentation, Utilization of past experiences, plot and the stories from their own life and psychological understanding (attraction, attention, rationality). Communicating a message through entertainment was the practice of this model. Finally impact analysis confirmed the success of the model. 展开更多
关键词 traditional forms of communication community empowerment development communication Sri lankan Folk-Lore
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Life Narratives of the Lankan Other: An Exploratory Study of the Jaffna Community in “Project I Am”
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作者 Lakshani Willarachchi 《Journalism and Mass Communication》 2017年第9期529-535,共7页
Life narrative is a field which is currently drawing increasing academic interest in Sri Lanka, though life narratives are an integral part of the socio-political fabric of the nation. In the Sri Lankan, context biogr... Life narrative is a field which is currently drawing increasing academic interest in Sri Lanka, though life narratives are an integral part of the socio-political fabric of the nation. In the Sri Lankan, context biography and autobiography are commonly seen as two separate genres in life writing, but certain life narratives tend to blur those boundaries. From This Point Forward, a photography exhibition by Luka Alagiyawanna, My Mother’s Village, a documentary life narrative by Aaron Burton, and Project I Am by Kannan Arunasalam are some examples. The present study analyses life narratives from I Am Jaffna section in Project I Am1; a digital archive of life narratives of Sri Lankan elders. Based on the life narratives, memories, and experiences of Sri Lankan elders, the project strives to find out whether there was a time when people in Sri Lanka did not describe themselves as Sinhalese, Tamil, Muslim, or Burgher, or a time when ethnic identity was not the primary defining factor of an individual. The reviews and articles on the projects have looked at the project merely as a repository of stories that help Sri Lankans understand their differences and a project promoting reconciliation with hardly any focus on the project as a Sri Lankan life narrative, so that a gap bridged by the present study. The study also focuses on the concept and politics of “othering” with a view to exploring how these life narratives are constructed as Sri Lankan life narratives. For this purpose, the study focuses on the segment on people from Jaffna-I am Jaffa, since the Jaffna community has a long standing history of being “the other.” The inseparability of the concept and politics ofothering and the identity of Sri Lankans show that it is extremely challenging to dispose of the notion of the other as it has actually become an integral aspect of Sri Lankan identity and a social reality. 展开更多
关键词 life writing Project I Am Sri lankan life narratives
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Recent Advances in Molecular Biological Applications in Sri Lankan Tea(Camellia sinensis) Industry:An Overview on the Achievements During Last Ten Years 被引量:2
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作者 K Mudith Mewan I Sarath B Abeysinghe 《茶叶》 2013年第4期267-278,共12页
For tea,which is predominantly out-breeding woody perennial,genetic improvement through conventional approaches is relatively ineffective,slow and costly.As a potential tool to enhance the process,Tea Research Institu... For tea,which is predominantly out-breeding woody perennial,genetic improvement through conventional approaches is relatively ineffective,slow and costly.As a potential tool to enhance the process,Tea Research Institute of Sri Lanka(TRISL) has integrated molecular biology to supplement the conventional program and is in progress.To date,a considerable progress has been achieved in the key areas such as genetic characterization and estimation of genetic diversity,isolation and characterization of EST and genomic SSRs,construction of SSR primers,construction of genetic maps and marker assisted selection and application of genomic approaches to understand the role of secondary metabolites disease resistance in tea and in this paper,a summarized version of above studies is presented. 展开更多
关键词 生物学应用 分子生物学 斯里兰卡 茶树 分子标记辅助选择 基因组学方法 多年生木本植物 茶叶研究所
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Sri Lankan black tea(Camellia sinensis L.)inhibits the methylglyoxal mediated protein glycation and potentiates its reversing activity in vitro
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作者 Walimuni Kanchana Subhashini Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya +1 位作者 Wanigasekara Daya Ratnasooriya Sirimal Premakumara Galbada Arachchige 《Journal of Coastal Life Medicine》 2016年第2期148-153,共6页
Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pe... Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments. 展开更多
关键词 METHYLGLYOXAL Advanced glycation end products Glycation reversing Diabetes complications Orange pekoe grade black tea Sri lankan tea
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Determination of Sex from the Tibia in a Contemporary Sri Lankan Population
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作者 Deepthi Nanayakkara Amal Nishantha Vadysinghe +1 位作者 Lakshika SNawarathna Harshana Sampath 《Journal of Forensic Science and Medicine》 2019年第1期24-28,共5页
Determination of sex from the tibial dimensions has been attempted before in different populations.The formulae developed to determine sex of one population are not appropriate to be applied to another.Seven standard ... Determination of sex from the tibial dimensions has been attempted before in different populations.The formulae developed to determine sex of one population are not appropriate to be applied to another.Seven standard variables including the maximum length of tibia,proximal epiphyseal breadth,distal epiphyseal breadth,minimum circumference of shaft,anteroposterior diameter at nutrient foramen,transverse diameter at the nutrient foramen(TDNF),and circumference at the nutrient foramen obtained from 81 adult tibiae(56 male and 25 female)were analyzed to investigate the sexual dimorphism in the tibial dimensions to establish sex estimating formulae from the tibia in a contemporary Sri Lankan population.Results confirmed the existence of sexual dimorphism of the tibia.Discriminant functions obtained resulted in the classification accuracies ranging from 61.9%to 80.2%.The most dimorphic single parameter in males was the TDNF providing an accuracy of 92.9%,while in females,the minimum circumference of shaft provided an accuracy of 70.4%.The best multivariate equation utilizing two tibial dimensions resulted in an accuracy of 80.2%after cross‑validation.We envisage that sex estimating formulae established in this study for a contemporary Sri Lankan population will contribute toward the biological profiling and identification of unknown skeletal remains. 展开更多
关键词 Discriminant function analysis forensic anthropology sex determination Sri lankans TIBIA
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Sri Lankan Ambassador to China Nihal Rodrigo recently spoke to Beijing Review, about the current situation between the government and the LTTE.
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《Beijing Review》 2006年第22期16-17,共2页
Last week, Chinese Premier Wen Jiabao headed a delegation to New Zealand, which was part of his South Pacific trip, to discuss trade and a potential free trade area between the two countries. Ahead of the visit, Beiji... Last week, Chinese Premier Wen Jiabao headed a delegation to New Zealand, which was part of his South Pacific trip, to discuss trade and a potential free trade area between the two countries. Ahead of the visit, Beijing Review spoke with New Zealand Ambassador to China Tony Browne on his country's trade relationship with China. 展开更多
关键词 Sri lankan Ambassador to China Nihal Rodrigo recently spoke to Beijing Review
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A Sri Lanka Native & China Lover The Deeds of Sri Lankan Friend Indrananda Abeysekera
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作者 Jiao Tianjiao 《Voice of Friendship》 2017年第3期16-18,共3页
'In my childhood,I read articles about Chinese master Monk Faxian’s travels in Sri Lanka and took interest in Chinese and its culture.Later,I knew Premier Zhou Enlai,who had been a friend of the Sri Lanka people ... 'In my childhood,I read articles about Chinese master Monk Faxian’s travels in Sri Lanka and took interest in Chinese and its culture.Later,I knew Premier Zhou Enlai,who had been a friend of the Sri Lanka people and helped us when we were in dire need of outside support.In my view,China is like the big brother of Sri Lanka.'—Indrananda 展开更多
关键词 In A Sri Lanka Native China Lover The Deeds of Sri lankan Friend Indrananda Abeysekera GREEK
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斯里兰卡木薯花叶病毒TaqMan荧光定量PCR检测方法的建立及应用 被引量:1
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作者 黄境珊 王国芬 +6 位作者 时涛 李超萍 陈奕鹏 蔡吉苗 李博勋 刘先宝 黄贵修 《热带作物学报》 北大核心 2025年第9期2056-2062,共7页
由斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,SLCMV)侵染引起的斯里兰卡木薯花叶病是近年来我国木薯种植中新发的危险性病害。现有检测技术存在灵敏度低、效率不高等不足,限制了相关工作的开展。本研究根据病毒基因序列设... 由斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,SLCMV)侵染引起的斯里兰卡木薯花叶病是近年来我国木薯种植中新发的危险性病害。现有检测技术存在灵敏度低、效率不高等不足,限制了相关工作的开展。本研究根据病毒基因序列设计引物和探针,制备阳性质粒标准品,建立SLCMV的TaqMan荧光定量检测技术,并对其应用效果等进行验证。结果发现,该方法仅对SLCMV DNA样品产生特异性荧光信号,对阳性质粒标准品的最低检出量为4.5×10^(1)copies/μL。标准曲线显示,Ct值与拷贝数的对数呈良好线性关系,曲线斜率为-3.1312,相关系数(R^(2))为0.9969,扩增效率(E)为97.9%,标准曲线方程为y=-3.1312x+34.599。利用该技术对广西和福建的2个木薯种植园供试样品进行检测,叶片阳性检出率分别达95.45%和78.57%,最低检测拷贝数为1.45×10^(5)copies/g,而田间烟粉虱携毒率为86%,最低带毒量为9.42×10^(4)copies/头。该技术具有良好的灵敏度、特异性和重复性,可为该病的田间鉴定、早期诊断、无毒种茎评价等监控工作提供有效的技术支持。 展开更多
关键词 木薯 斯里兰卡木薯花叶病毒 TaqMan荧光定量PCR
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斯里兰卡木薯花叶病毒在亚洲的暴发、传播与防控 被引量:1
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作者 王琪琦 潘冉冉 +1 位作者 王亚杰 王晓伟 《生物安全学报(中英文)》 北大核心 2025年第1期20-26,共7页
木薯是重要的经济与粮食作物,由于其出色的耐旱、耐贫瘠、易种植等特性,在全球热带和亚热带地区广泛种植。木薯在生长过程中会受到多种病虫害的威胁,其中分布最广、危害最严重的是木薯花叶病毒(Cassava mosaic virus,CMV)。CMV主要通过... 木薯是重要的经济与粮食作物,由于其出色的耐旱、耐贫瘠、易种植等特性,在全球热带和亚热带地区广泛种植。木薯在生长过程中会受到多种病虫害的威胁,其中分布最广、危害最严重的是木薯花叶病毒(Cassava mosaic virus,CMV)。CMV主要通过带毒茎秆和媒介昆虫烟粉虱传播,可以造成木薯叶片出现斑驳花叶、卷曲甚至枯萎,植株整体长势衰弱,导致木薯产量下降,严重威胁木薯生产。我国和东南亚木薯产区此前并没有CMV发生和危害的报道。可近年来,斯里兰卡木薯花叶病毒(Sri Lanka cassava mosaic virus,SLCMV)在东南亚地区广泛传播,已经成为该地区木薯生产的主要病害,对我国的木薯生产也构成了重大威胁。本文围绕SLCMV在亚洲的发生、传播机制和防治策略展开了讨论,以期为防控SLCMV在我国的危害提供参考。 展开更多
关键词 木薯 斯里兰卡木薯花叶病毒 烟粉虱 抗性育种 综合防治
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我国木薯花叶病毒病的发生危害及其病原鉴定 被引量:11
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作者 王国芬 李超萍 +5 位作者 时涛 周司珊 李博勋 蔡吉苗 林兆威 黄贵修 《热带作物学报》 CSCD 北大核心 2021年第6期1668-1677,共10页
为明确国内木薯花叶病毒病(cassava mosaic virus disease)的危害情况、田间症状及其病原类型,本研究于2018—2019年调查了该病在国内的分布情况、症状特征及种质来源等数据,采集了8省(区)19地的384份样品,利用特异性引物检测2种木薯花... 为明确国内木薯花叶病毒病(cassava mosaic virus disease)的危害情况、田间症状及其病原类型,本研究于2018—2019年调查了该病在国内的分布情况、症状特征及种质来源等数据,采集了8省(区)19地的384份样品,利用特异性引物检测2种木薯花叶病毒侵染引起的症状类型,并通过序列比对明确其病原特点。结果表明:木薯花叶病毒病已经在我国发生,种茎调运是该病远距离传播的主要原因;国内木薯花叶病毒病病原有斯里兰卡木薯花叶病毒株系(Sri Lankan cassava mosaic virus,SLCMV)和木薯普通花叶病毒(Cassava common mosaic virus,CsCMV)2种病毒,可分为单独感染、复合感染,其中SLCMV的检出率为21.1%,CsCMV的检出率为36.5%,SLCMV-CsCMV复合侵染率为15.1%;检测到的SLVMV与东南亚SLCMV序列同源性为99.4%~99.7%,检测到的CsCMV与拉丁美洲的CsCMV序列相似性为91%~96%。推测国内木薯花叶病毒病主要由2种病毒侵染引起,且普通花叶病毒有产生序列变异的可能性。 展开更多
关键词 木薯 斯里兰卡木薯花叶病毒(SLCMV) 木薯普通花叶病毒(CsCMV) 检测 复合侵染
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斯里兰卡学生汉语四声习得的调查分析 被引量:5
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作者 杨刚 朱珠 《云南师范大学学报(对外汉语教学与研究版)》 2013年第4期63-68,共6页
斯里兰卡的汉语教学起步较晚,目前,针对斯里兰卡人学习汉语的国别调查与研究分析在学界较为少见。文章以斯里兰卡凯拉尼亚大学中文系学生为样本,运用实验语音学的基本方法调查分析其汉语四声发音情况。调查发现:斯里兰卡学生发汉语普通... 斯里兰卡的汉语教学起步较晚,目前,针对斯里兰卡人学习汉语的国别调查与研究分析在学界较为少见。文章以斯里兰卡凯拉尼亚大学中文系学生为样本,运用实验语音学的基本方法调查分析其汉语四声发音情况。调查发现:斯里兰卡学生发汉语普通话四声,具有音高偏低,调域偏窄的总体特征。四声中,学习难度最大的是上声,学生常将其割裂为"降调+升调+短促降调"的组合,听觉感受非常生硬;其次为阳平,通常将其发为降调或"降调+升调"的组合;较易掌握的是阴平和去声。在连续语流中进行声调组合时学生既受僧伽罗语语调影响,也受前后声调的同化、异化影响,加上汉语语音变调的要求,总体较混乱。偏误率超过五成的依次是"阴平+上声","上声+上声","去声+阴平"和"阳平+阳平"等组合。 展开更多
关键词 斯里兰卡学生 汉语四声 偏误分析 教学策略
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“一带一路”汉班托塔港项目的舆论生态及其影响因素分析 被引量:4
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作者 欧亚 刘星瑞 《国际论坛》 CSSCI 北大核心 2021年第5期76-90,157,158,共17页
作为连接东西方的航运和商贸中心,斯里兰卡汉班托塔港是“一带一路”倡议的重点项目之一,也是国际舆论关注的焦点。与西方国家普遍将汉班托塔港项目建构为“中国为小国制造债务陷阱”或“中国掠夺性政策”的负面舆论不同,斯里兰卡媒体... 作为连接东西方的航运和商贸中心,斯里兰卡汉班托塔港是“一带一路”倡议的重点项目之一,也是国际舆论关注的焦点。与西方国家普遍将汉班托塔港项目建构为“中国为小国制造债务陷阱”或“中国掠夺性政策”的负面舆论不同,斯里兰卡媒体认可这一项目发挥了促进斯里兰卡经济发展与推进中斯两国关系的正向效应,在“中国债务陷阱论”“港口股份所有权”“港口项目加剧大国博弈”等议题上反映了各利益相关方的政治博弈与话语竞争,展现了复杂、多元的舆论生态。媒体报道显示,斯里兰卡对中斯加深合作既有期待也有疑虑。斯里兰卡民众怀有的“小国对大国处于弱势”的心理倾向、斯里兰卡国内政治局势变化以及大国博弈是影响港口项目舆论生态的内外动因,但项目本身的进展与成效仍是其中的决定性因素。后疫情时期,中国针对汉班托塔港等关键项目需要“软硬并进”,在调整“一带一路”倡议的建设模式、妥善处理债务问题等工作的基础上,改变被动应对西方舆论的局面,为推进更加透明、更加具有可持续性和共商共建的“一带一路”建设营造良好的舆论环境。 展开更多
关键词 “一带一路”倡议 汉班托塔港 斯里兰卡舆论 债务陷阱 大国博弈
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岛国发展困局与中斯经济合作 被引量:5
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作者 陈利君 《南亚东南亚研究》 2022年第3期92-107,155,156,共18页
岛国与内陆国、沿海国的地理、资源、市场容量等条件不同,其发展道路也不尽相同。世界上岛国众多,但大多数都面临一定的“发展困局”。斯里兰卡是印度洋岛国,长期奉行独立自主的外交政策,同世界建立了广泛联系。中国与斯里兰卡虽然地理... 岛国与内陆国、沿海国的地理、资源、市场容量等条件不同,其发展道路也不尽相同。世界上岛国众多,但大多数都面临一定的“发展困局”。斯里兰卡是印度洋岛国,长期奉行独立自主的外交政策,同世界建立了广泛联系。中国与斯里兰卡虽然地理位置较远,但友好交往源远流长。近年来,中斯在“一带一路”框架下开展全方位合作,合作成果丰硕,双边关系迈上新台阶。目前斯里兰卡受俄乌冲突、疫情、国际大宗商品价格猛涨等外溢因素的影响,正面临着严重的外汇危机、能源危机、粮食危机、药品危机和经济危机,经济发展、人民生活、社会稳定受到前所未有的挑战。中国积极发展中斯关系,努力推进经济合作,并在力所能及的范围之内为斯提供各种帮助,这对斯里兰卡经济社会发展和民生改善提供了强有力的支持,获得了斯各界的广泛认可。斯里兰卡要恢复往日的“亚洲之虎”荣光,既需要政局与社会稳定、深化改革、振兴产业,增强内生发展动力,又需要扩大开放、优化环境、保持“外交平衡”,以充分利用国内外资源促进发展。中斯可借建交65周年和《米胶协定》签署70周年之机,承前启后,进一步深化务实合作,高质量共建“一带一路”,共同开启友好合作新篇章。 展开更多
关键词 “一带一路” 岛国经济 岛国困局 经济危机 中斯合作
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内外之间,即景生情——中国古代建筑、文学与艺术中的栏杆 被引量:8
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作者 李路珂 《世界建筑》 2018年第9期18-23,共6页
本文以宋《营造法式》的"钩阑"和"阑槛钓窗"制度为中心,从起源、演变、意义和形式四个方面探讨了中国古代建筑、文学与艺术中"栏杆"的内涵。
关键词 栏杆 扶手 《营造法式》 小木作 石作 钩阑 勾栏 阑槛钓窗
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我国斯里兰卡木薯花叶病毒的分子特征及致病性分析
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作者 王国芬 李超萍 +5 位作者 时涛 吴会杰 蔡吉苗 李博勋 陆翠梅 黄贵修 《热带作物学报》 CSCD 北大核心 2024年第1期187-196,共10页
为进一步探究我国斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,SLCMV)的分子特征及致病性。以感染SLCMV的木薯叶片基因组DNA为模板,PCR扩增获得DNA-A和DNA-B基因组全长,通过生物信息软件分析比较其核酸及氨基酸序列特征;构... 为进一步探究我国斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,SLCMV)的分子特征及致病性。以感染SLCMV的木薯叶片基因组DNA为模板,PCR扩增获得DNA-A和DNA-B基因组全长,通过生物信息软件分析比较其核酸及氨基酸序列特征;构建了强、弱致病力分离物(SLCMV-Colombo和SLCMV-DG1922)的侵染性克隆,分别将2种致病力分离物的DNA-A和DNA-B组分进行重组,接种烟草(Nicotianatabacum),比较2种分离物的致病性差异。结果显示:我国SLCMV为“旧世界”双组份菜豆花叶病毒,编码包括AV2基因在内的8个开放阅读框(ORFs);具有双生病毒典型的共同序列(CR)、重复序列、TATABox和TAATATT↓AC茎环结构,Rep蛋白羧基末端有7个氨基酸缺失;我国SLCMV的基因组、编码和非编码区与柬埔寨、泰国和越南分离物的相似性在97.0%~100.0%之间,与印度和斯里兰卡早期的分离物相似性为86.5%~98.6%,DNA-B组份与印度木薯花叶病毒株系(ICMV)编码区序列相似性为95.0%~97.6%,与其他9个非洲木薯花叶病毒株系序列之间的序列相似性均低于80.0%。侵染性克隆接种结果证实,强致病力分离物SLCMV-Col的DNA-A(Col-A)组份在烟草叶片表现典型花叶症状中起主要作用,而我国分离物的DNA-B(DG1922-B)能协同Col-A对烟草产生强致病性。本研究分析了我国SLCMV的全基因组结构,建立的侵染性克隆及其技术为进一步解析SLCMV致病性机理提供重要的研究基础。 展开更多
关键词 斯里兰卡木薯花叶病毒 分子特征 侵染性克隆 致病性
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超声辅助-GC-MS联用分析斯里兰卡桂叶油挥发性成分
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作者 崔成哲 付祺 +3 位作者 杨泽恩 李宝志 梁淼 李河霖 《农产品加工》 2024年第6期64-66,共3页
采用超声辅助萃取结合气相色谱-质谱联用法(Gas chromatography-mass spectrometry,GC-MS)分析斯里兰卡桂叶油中挥发性成分。结果表明,①斯里兰卡桂叶油中共检测出32种挥发性成分,包含烯烃类、酚类、酯类、醇类、醛酮类、杂环类化合物;... 采用超声辅助萃取结合气相色谱-质谱联用法(Gas chromatography-mass spectrometry,GC-MS)分析斯里兰卡桂叶油中挥发性成分。结果表明,①斯里兰卡桂叶油中共检测出32种挥发性成分,包含烯烃类、酚类、酯类、醇类、醛酮类、杂环类化合物;②其中酚类化合物总含量最高,为23.05 mg/g,占总挥发性物质的81.10%;③斯里兰卡桂叶油中含量较高的物质为丁香酚(23.05 mg/g)、苯甲酸苄酯(1.04 mg/g)、芳樟醇(0.53 mg/g)、氧化石竹烯(0.43 mg/g)、反式肉桂醛(0.15 mg/g)。 展开更多
关键词 斯里兰卡桂叶油 气相色谱-质谱联用法 挥发性成分 超声辅助萃取
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木薯花叶病毒AC4蛋白与AtPARN互作研究 被引量:4
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作者 刘琳玉 赵平娟 +3 位作者 符艳 刘志昕 任艳利 张秀春 《热带作物学报》 CSCD 北大核心 2024年第1期197-204,共8页
木薯(Manihot esculenta Crantz)作为重要的热带作物,是全球六大粮食作物之一,并为全球近七亿人口提供主粮,然而病毒侵染引起的病害在全球范围内严重威胁木薯产业的发展。木薯花叶病毒病(cassava mosaic disease,CMD)是最有威胁的病害之... 木薯(Manihot esculenta Crantz)作为重要的热带作物,是全球六大粮食作物之一,并为全球近七亿人口提供主粮,然而病毒侵染引起的病害在全球范围内严重威胁木薯产业的发展。木薯花叶病毒病(cassava mosaic disease,CMD)是最有威胁的病害之一,造成严重的经济损失。斯里兰卡木薯花叶病毒(SriLankancassavamosaicvirus,SLCMV)是引发木薯花叶病的病原物之一,SLCMV是典型的双组分双生病毒,其基因组由DNA-A和DNA-B两个环状组分组成。无义介导的mRNA降解(nonsense-mediatedmRNAdecay,NMD)是真核细胞mRNA降解的主要途径之一,也是真核生物普遍存在的抗病毒防御机制。越来越多的研究显示,NMD不仅是真核生物重要的mRNA数量、质量调控机制,还与转录后基因沉默(post transcriptional gene silencing,PTGS)同样能降解病毒RNA,是真核生物普遍存在的抗病毒防御机制。NMD的mRNA衰减过程包括PTC的识别、脱腺苷酸、脱帽和最后的核酸外切酶降解4个过程,UPF1、PARN、DCP2和XRN4分别是上述4个过程中的关键蛋白。病毒是专性寄生生物,必须逃避或耐受寄主细胞的降解,才能成功感染。聚腺苷酸特异性核糖核酸酶[poly(A)-specific ribonuclease,PARN]是NMD信号通路的一个关键因子。目前,关于SLCMV抵御寄主NMD的分子机制尚不明确。本研究采用酵母双杂交(yeast two-hybrid system)和荧光双分子互补(bimolecular fluorescence complementation,BiFC)试验证明斯里兰卡木薯花叶病毒(SLCMV)编码的沉默抑制子AC4与拟南芥PARN相互作用。据此推测SLCMV AC4蛋白可能通过与AtPARN相互作用而抑制寄主NMD的病毒防御功能帮助病毒逃避或耐受寄主细胞的降解。研究结果为阐明木薯花叶病毒调控寄主NMD抗病毒防御功能的分子机理奠定基础。 展开更多
关键词 斯里兰卡木薯花叶病毒(SLCMV) AC4蛋白 聚腺苷酸特异性核糖核酸酶(PARN) 酵母双杂交(Y2H) 荧光双分子互补(BiFC)
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斯里兰卡木薯花叶病毒AC2与拟南芥SGS3蛋白互作研究 被引量:1
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作者 刘雪婷 谢秋贤 +3 位作者 刘琳玉 符艳 张秀春 任艳利 《热带作物学报》 CSCD 北大核心 2024年第7期1323-1331,共9页
木薯花叶病毒病(cassava mosaic disease,CMD)对我国和世界木薯生产造成严重威胁和危害,目前尚无有效防治方法且缺乏抗性材料,造成严重经济损失,被认为是最有威胁性的病毒病之一,斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,... 木薯花叶病毒病(cassava mosaic disease,CMD)对我国和世界木薯生产造成严重威胁和危害,目前尚无有效防治方法且缺乏抗性材料,造成严重经济损失,被认为是最有威胁性的病毒病之一,斯里兰卡木薯花叶病毒(Sri Lankan cassava mosaic virus,SLCMV)是引起木薯花叶病的主要病原之一。RNA沉默是植物和动物先天性的一种抗病毒免疫反应机制,寄主转录后基因沉默(post transcriptional gene silencing,PTGS)是真核生物中一种保守的基因表达调控机制,也是重要的抗病毒免疫机制之一。寄主基因沉默抑制子(suppressor of gene silencing 3,SGS3)是PTGS通路的关键蛋白,在寄主抵御病毒侵染中发挥着关键作用。为抵御宿主这种免疫反应,病毒通常编码病毒沉默抑制子(viral suppressors of RNA silencing,VSRs)来抵御寄主PTGS的病毒防御机制,与寄主蛋白互作是沉默抑制子发挥作用的主要作用机制之一。为探究SLCMV AC2是否通过与AtSGS3互作而抑制寄主PTGS的免疫功能,本研究采用酵母双杂交技术(yeast two hybrid system,Y2H)和双分子荧光互补技术(bimolecular fluorescence complementation,BiFC)分析SLCMV AC2与AtSGS3之间的相互作用。结果表明:SLCMV AC2与AtSGS3蛋白在酵母细胞和植物体内均存在相互作用的关系。亚细胞共定位研究进一步证明SLCMV AC2在与AtSGS3互作后,可与AtSGS3形成siRNA-body的RDR6发生共定位。这些结果表明SLCMV AC2可能通过与siRNA-body互作而影响寄主自身免疫机制,提高病毒致病性,SLCMV AC2与AtSGS3的互作可能是SLCMV侵染木薯导致花叶症状发生的分子基础。该研究结果为后续深入解析SLCMV致病机理提供新的理论依据。 展开更多
关键词 斯里兰卡木薯花叶病毒 SLCMV AC2 AtSGS3 蛋白互作
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