A reversed phase high performance liquid chromatography （HPLC） method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo Cl8 col...A reversed phase high performance liquid chromatography （HPLC） method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo Cl8 column （250 mm × 4.6 mm, 5 μm） was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid （0.2%, v/v）. Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 ℃. An ultraviolet （UV） detector was used with a selected wavelength of 240 nm. Calibration curves were linear within the concentration range of 4.6-45.75 μg/mL for 12, 13-dihydroxyeuparin （r〉0.9999） and 106.9-1068.9μg/mL for glycyrrhizic acid （r〉0.9999）, respectively. Recoveries were 102.18% for 12, 13-dihydroxyeuparin and 101.17% for glycyrrhizic acid. The method developed could be applied to the simultaneous determination of 12, 13- dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.展开更多
Sets of cold-filled SMA-13 asphalt mixture were designed by means of orthogonal design method. The bending and low temperature creep tests of the cold-filled SMA-13 asphalt mixture were carried out. The related models...Sets of cold-filled SMA-13 asphalt mixture were designed by means of orthogonal design method. The bending and low temperature creep tests of the cold-filled SMA-13 asphalt mixture were carried out. The related models of the fractal dimension and the road performance evaluation index including low temperature bending failure strain εB and bending strength RB are established by using fractal theory. The model can be used to predict the low temperature performance of cold-filled SMA-13 asphalt mixture according to the design gradation, which can reduce the test workload and improve the working efficiency, so as to provide the reference for engineering design.展开更多
基金supported by Guangdong Natural Product Reference Material Research & Development Central Lab,the First Affiliate Hospital of Sun Yat-sen University and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No:2010B090400533)
文摘A reversed phase high performance liquid chromatography （HPLC） method was established for the simultaneous determination of 12, 13-dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture. A Grace Apollo Cl8 column （250 mm × 4.6 mm, 5 μm） was used as the stationary phase and the mobile phase was composed of acetonitrile and aqueous phosphoric acid （0.2%, v/v）. Gradient elution was carried out at the flow rate of 1.0 mL/min and the column temperature was 30 ℃. An ultraviolet （UV） detector was used with a selected wavelength of 240 nm. Calibration curves were linear within the concentration range of 4.6-45.75 μg/mL for 12, 13-dihydroxyeuparin （r〉0.9999） and 106.9-1068.9μg/mL for glycyrrhizic acid （r〉0.9999）, respectively. Recoveries were 102.18% for 12, 13-dihydroxyeuparin and 101.17% for glycyrrhizic acid. The method developed could be applied to the simultaneous determination of 12, 13- dihydroxyeuparin and glycyrrhizic acid in Yanyanfang mixture.
文摘Sets of cold-filled SMA-13 asphalt mixture were designed by means of orthogonal design method. The bending and low temperature creep tests of the cold-filled SMA-13 asphalt mixture were carried out. The related models of the fractal dimension and the road performance evaluation index including low temperature bending failure strain εB and bending strength RB are established by using fractal theory. The model can be used to predict the low temperature performance of cold-filled SMA-13 asphalt mixture according to the design gradation, which can reduce the test workload and improve the working efficiency, so as to provide the reference for engineering design.
