Dysregulated inflammation and multi-organ failure are hallmarks of sepsis,a potentially fatal illness for which there are currently no effective treatments.Fatty acid-binding protein(A-FABP)has been identified in rece...Dysregulated inflammation and multi-organ failure are hallmarks of sepsis,a potentially fatal illness for which there are currently no effective treatments.Fatty acid-binding protein(A-FABP)has been identified in recent research as a crucial mediator of the inflammatory pathways underlying sepsis.In this study,we used a murine model of lipopolysaccharide(LPS)-induced endotoxemia to assess the therapeutic potential of 6H2,a monoclonal antibody that targets A-FABP.In comparison to untreated septic mice,6H2 treatment significantly increased survival rates,decreased histopathological damage in the liver,lungs,kidneys,and heart,and reduced systemic inflammation.According to biochemical analyses,6H2 treatment decreased circulating levels of A-FABP,and this was associated with a reduction in inflammatory markers.These results indicate that A-FABP inhibition is a potentially effective treatment approach for sepsis,with 6H2 demonstrating strong therapeutic efficacy.展开更多
针对风机偏航转速传感器在长期运行中受偏航旋转工况影响易出现供电不连续与电压波动的问题,开展基于有限电源(Limited Power Source,LPS)冗余供电的稳定性改造应用研究。通过在传感器供电侧引入LPS冗余供电方式,从供电连续性增强、供...针对风机偏航转速传感器在长期运行中受偏航旋转工况影响易出现供电不连续与电压波动的问题,开展基于有限电源(Limited Power Source,LPS)冗余供电的稳定性改造应用研究。通过在传感器供电侧引入LPS冗余供电方式,从供电连续性增强、供电波动抑制及转速信号稳定输出3个方面分析LPS冗余供电在稳定性改造方面的应用,并结合工程实例对改造效果进行对比分析。结果表明,引入LPS冗余供电能够有效提升偏航转速传感器运行稳定性,具有良好的工程应用价值。展开更多
目的:由连梅汤(LMD)对脂多糖(LPS)诱导的脓毒症小鼠回肠屏障及肝肺损伤的保护作用的研究。方法:采用随机方式,将C57/6J雄性小鼠分为对照组(CON组)、LPS模型组(LPS组)和连梅汤治疗组(LMD组),每组各6只。适应性喂养7天后,分别经口给予生...目的:由连梅汤(LMD)对脂多糖(LPS)诱导的脓毒症小鼠回肠屏障及肝肺损伤的保护作用的研究。方法:采用随机方式,将C57/6J雄性小鼠分为对照组(CON组)、LPS模型组(LPS组)和连梅汤治疗组(LMD组),每组各6只。适应性喂养7天后,分别经口给予生理盐水与治疗剂量的连梅汤(LMD) 21天。第22天,PBS注入对照组腹腔,另两组LPS注入5 mg/kg腹腔,建立脓毒症模型小鼠。腹腔注射24小时后进行回肠和肝、肺组织的收集。用HE染色组织病理鉴定;通过RT-qPCR检测回肠屏障因子水平(ZO-1, Occludin)和肝肺组织炎性因子(IL-1α, IL-8, TNF-α)。结果LPS组与CON相比,体重下降明显(n = 6;P β、IL-8、TNF-α)水平(n = 6;P Objective: The protective effect of Lianmei Decoction on the intestine was studied to the intestinal barrier, liver, and lung damage caused by lipopolysaccharide (LPS) in septic mice. Methods: C57/6J male mice were randomly assigned to the control group (CON group), LPS model group (LPS group), and Lianmei Decoction treatment group (LMD group), with 6 mice in each group. After 7 days of adaptive feeding, normal saline and a therapeutic dose of LMD were given orally for 21 days. In the 22 days of the study, PBS was injected intraperitoneally into the CON group, and 5 mg/kg LPS was administered intraperitoneally to the remaining two groups to create a sepsis mouse model. Ileum, liver, and lung tissues were gathered 24 hours after intraperitoneal injection. Histopathological examination was done using HE staining;the amounts of ileal barrier factors (ZO-1, Occludin) and inflammatory factors (IL-1β, IL-8, TNF-α) in liver and lung tissues were detected by RT-qPCR. Results: Compared with the CON group, the body weight of the LPS group decreased dramatically (n = 6;P β, IL-8, TNF-a) in liver and lung tissues (n = 6;P < 0.05), reduce the pathological damage of liver and pulmonary tissue. Conclusion: Lianmei Decoction can effectively improve ileal barrier damage, liver and lung injury, and inflammatory imbalance in LPS-induced sepsis mice.展开更多
文摘Dysregulated inflammation and multi-organ failure are hallmarks of sepsis,a potentially fatal illness for which there are currently no effective treatments.Fatty acid-binding protein(A-FABP)has been identified in recent research as a crucial mediator of the inflammatory pathways underlying sepsis.In this study,we used a murine model of lipopolysaccharide(LPS)-induced endotoxemia to assess the therapeutic potential of 6H2,a monoclonal antibody that targets A-FABP.In comparison to untreated septic mice,6H2 treatment significantly increased survival rates,decreased histopathological damage in the liver,lungs,kidneys,and heart,and reduced systemic inflammation.According to biochemical analyses,6H2 treatment decreased circulating levels of A-FABP,and this was associated with a reduction in inflammatory markers.These results indicate that A-FABP inhibition is a potentially effective treatment approach for sepsis,with 6H2 demonstrating strong therapeutic efficacy.
文摘针对风机偏航转速传感器在长期运行中受偏航旋转工况影响易出现供电不连续与电压波动的问题,开展基于有限电源(Limited Power Source,LPS)冗余供电的稳定性改造应用研究。通过在传感器供电侧引入LPS冗余供电方式,从供电连续性增强、供电波动抑制及转速信号稳定输出3个方面分析LPS冗余供电在稳定性改造方面的应用,并结合工程实例对改造效果进行对比分析。结果表明,引入LPS冗余供电能够有效提升偏航转速传感器运行稳定性,具有良好的工程应用价值。
文摘目的:由连梅汤(LMD)对脂多糖(LPS)诱导的脓毒症小鼠回肠屏障及肝肺损伤的保护作用的研究。方法:采用随机方式,将C57/6J雄性小鼠分为对照组(CON组)、LPS模型组(LPS组)和连梅汤治疗组(LMD组),每组各6只。适应性喂养7天后,分别经口给予生理盐水与治疗剂量的连梅汤(LMD) 21天。第22天,PBS注入对照组腹腔,另两组LPS注入5 mg/kg腹腔,建立脓毒症模型小鼠。腹腔注射24小时后进行回肠和肝、肺组织的收集。用HE染色组织病理鉴定;通过RT-qPCR检测回肠屏障因子水平(ZO-1, Occludin)和肝肺组织炎性因子(IL-1α, IL-8, TNF-α)。结果LPS组与CON相比,体重下降明显(n = 6;P β、IL-8、TNF-α)水平(n = 6;P Objective: The protective effect of Lianmei Decoction on the intestine was studied to the intestinal barrier, liver, and lung damage caused by lipopolysaccharide (LPS) in septic mice. Methods: C57/6J male mice were randomly assigned to the control group (CON group), LPS model group (LPS group), and Lianmei Decoction treatment group (LMD group), with 6 mice in each group. After 7 days of adaptive feeding, normal saline and a therapeutic dose of LMD were given orally for 21 days. In the 22 days of the study, PBS was injected intraperitoneally into the CON group, and 5 mg/kg LPS was administered intraperitoneally to the remaining two groups to create a sepsis mouse model. Ileum, liver, and lung tissues were gathered 24 hours after intraperitoneal injection. Histopathological examination was done using HE staining;the amounts of ileal barrier factors (ZO-1, Occludin) and inflammatory factors (IL-1β, IL-8, TNF-α) in liver and lung tissues were detected by RT-qPCR. Results: Compared with the CON group, the body weight of the LPS group decreased dramatically (n = 6;P β, IL-8, TNF-a) in liver and lung tissues (n = 6;P < 0.05), reduce the pathological damage of liver and pulmonary tissue. Conclusion: Lianmei Decoction can effectively improve ileal barrier damage, liver and lung injury, and inflammatory imbalance in LPS-induced sepsis mice.
