摘要
目的研究壮药火钱胶囊对D-氨基半乳糖/脂多糖(D-GalN/LPS)所致急性肝衰竭小鼠的保护作用。方法小鼠随机分为正常组、模型组、水飞蓟宾组(100 mg/kg)和火钱胶囊低、中、高剂量组(365、730、1460 mg/kg),每组25只。预防性灌胃给药10 d后,采用单次腹腔注射给予D-GalN(700 mg/kg)和LPS(10μg/kg)建立肝衰竭模型。检测小鼠存活率;计算肝指数;检测血清AST、ALT、IL-1β、IL-6、TNF-α、MCP-1水平,肝组织MDA、NO、IL-1β、IL-6、TNF-α水平和SOD活性;HE染色观察肝组织病理学改变,RT-qPCR法检测肝组织IL-1β、IL-6、TNF-α、iNOS、NF-κB、IκBα、TLR 4 mRNA表达;Western blot法检测肝组织Bax、Bcl-2、Bcl-xL、p-p38、p-p65蛋白表达。结果与模型组比较,水飞蓟宾组和火钱胶囊各剂量组小鼠存活率提高,肝指数、血清AST、ALT、IL-1β、IL-6、TNF-α、MCP-1和肝组织MDA、NO、IL-1β、IL-6、TNF-α水平降低(P<0.05,P<0.01),肝组织SOD活性升高(P<0.01),肝组织病理损伤减轻;水飞蓟宾组和火钱胶囊高剂量组小鼠肝组织IL-1β、IL-6、TNF-α、iNOS、NF-κB、IκBα、TLR 4 mRNA和Bax、p-p38、p-p65蛋白表达降低(P<0.05,P<0.01)。结论火钱胶囊对D-GalN/LPS所致急性肝衰竭小鼠具有保护作用,其机制可能与抑制肝细胞炎症和凋亡有关。
AIM To investigate the protective effects of Zhuang-medicine Huoqian Capsules on acute liver failure(ALF)induced by D-galactosamine/lipopolysaccharide(D-GalN/LPS)in mice.METHODS The mice were randomly divided into the normal group,the model group,the silibinin group(100 mg/kg)and the low-dose,medium-dose and high-dose Huoqian Capsules groups(365,730,1460 mg/kg),with 25 mice in each group.After 10 days preventive intragastric administration of the corresponding drug,single intraperitoneal injection of D-GalN(700 mg/kg)and LPS(10μg/kg)was conducted to establish the mouse model of ALF.The mice had their survival rate examined;their liver index calculated;their serum levels of AST,ALT,IL-1β,IL-6,TNF-α and MCP-1 and hepatic levels of MDA,NO,IL-1β,IL-6 and TNF-α and SOD activity measured;their liver histopathological changed examined by HE staining;their hepatic mRNA expressions of IL-1β,IL-6,TNF-α,iNOS,NF-κB,IκBαand TLR 4 measured by RT-qPCR;and their hepatic protein expressions of Bax,Bcl-2,Bcl-xL,p-p38 and p-p65 measured by Western blot.RESULTS Compared with the model group,the groups intervened with either silibinin or Huoqian Capsules displayed increased survival rate;decreased levels of liver index,serum AST,ALT,IL-1β,IL-6,TNF-α,MCP-1 and hepatic MDA,NO,IL-1β,IL-6 and TNF-α(P<0.05,P<0.01);increased hepatic SOD activity(P<0.01);and alleviated pathological damage of the liver tissue.Additionally,the silibinin and high-dose Huoqian Capsules groups shared decreased hepatic expressions of IL-1β,IL-6,TNF-α,iNOS,NF-κB,IκBα,TLR 4 mRNA and Bax,p-p38,p-p65 proteins(P<0.05,P<0.01).CONCLUSION With their good hepatoprotective effects on the D-GalN/LPS-induced mouse model of ALF,Huoqian Capsules may inhibit the hepatocytic inflammation and apoptosis.
作者
王冰冰
朱华
谢凤凤
许立拔
韦安达
余秀琪
WANG Bing-bing;ZHU Hua;XIE Feng-feng;XU Li-ba;WEI An-da;YU Xiu-qi(Guangxi Key Laboratory for Zhuang and Yao Medicines,Collaborative Innovation Center for Zhuang and Yao Medicines,Guangxi Zhuang Autonomous Regional Engineering Research Center for Ethnic Medicine Resource and Application,Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《中成药》
北大核心
2026年第1期49-56,共8页
Chinese Traditional Patent Medicine
基金
广西重点研发计划项目(桂科AB21196016)
广西科技基地和人才专项(桂科AD21238031)
广西中医药重点学科壮药学(GZXK-Z-20-64)
广西一流学科中药学(民族药学)(桂教科研[2018]12号)。
