虚拟化技术日益成为关注的焦点,正在最大程度地改变企业 IT 基础设施的部署及运营。甘肃电力协同办公系统打破原有本部、各地市公司两级部署模式,采用大集中部署服务器的技术架构构建电力业务应用系统。 2 台 IBM p6 570 小型机采用逻...虚拟化技术日益成为关注的焦点,正在最大程度地改变企业 IT 基础设施的部署及运营。甘肃电力协同办公系统打破原有本部、各地市公司两级部署模式,采用大集中部署服务器的技术架构构建电力业务应用系统。 2 台 IBM p6 570 小型机采用逻辑分区技术(LPAR),将单台服务器划分成多个逻辑服务器,彼此运行独立的应用程序。逻辑分区技术在甘肃电力协同办公系统中的应用,不仅可以减少服务器的数量,通过降低空间、散热以及电力消耗等途径提供一种服务器整合的方法,节省成本投资;而且可以提高可用性,带来具有透明负载均衡、动态迁移、故障自动隔离、系统自动重构的高可靠服务器应用环境。展开更多
目的探讨miR-15b靶向LPAR3调控食管癌细胞增殖、迁移及侵袭的影响。方法取对数生长期的人食管癌Eca109细胞分为对照组、miR-15b mimic组、miR-NC组、miR-15b mimic+pcDNA3.1组、miR-15b mimic+pcDNA-LPAR3组;另设对数期生长的人食管上...目的探讨miR-15b靶向LPAR3调控食管癌细胞增殖、迁移及侵袭的影响。方法取对数生长期的人食管癌Eca109细胞分为对照组、miR-15b mimic组、miR-NC组、miR-15b mimic+pcDNA3.1组、miR-15b mimic+pcDNA-LPAR3组;另设对数期生长的人食管上皮细胞HEEpiC为空白组。采用qRT-PCR检测各组细胞中miR-15b和LPAR3 mRNA的表达;应用MTT法、划痕实验和Transwell小室实验分别检测各组细胞的增殖、迁移和侵袭情况;Western blot检测Eca109细胞中LPAR3和增殖、迁移、侵袭相关蛋白(Cyclin D1、MMP-2、MMP-9)的表达。结果与空白组相比,对照组Eca109细胞中miR-15b水平明显降低(1.00±0 vs 0.42±0.03,t=33.486,P<0.01),LPAR3 mRNA水平显著升高(1.00±0 vs 2.33±0.15,t=15.358,P<0.01);与对照组相比,miR-15b mimic组细胞miR-15b水平显著升高(0.42±0.03 vs 4.61±0.38,t=19.039,P<0.01),LPAR3 mRNA(2.33±0.15 vs 1.29±0.13)和蛋白(1.12±0.13 vs 0.35±0.03)水平、细胞增殖率(100.00±0 vs 64.57±6.11)、划痕愈合率(63.45±7.20 vs 36.52±4.29)、Eca109细胞进入Transwell小室下层的数量(155.31±14.23 vs 101.33±11.45)、Cyclin D1(0.85±0.10 vs 0.38±0.05)、MMP-2(0.76±0.08 vs 0.33±0.04)、MMP-9(0.94±0.10 vs 0.41±0.05)蛋白表达均显著降低(t=9.075、9.996、10.044、5.565、5.119、7.281、8.327、8.211,P<0.01);使用pcDNA-LPAR3过表达LPAR3能明显逆转miR-15b mimic对细胞增殖、迁移和侵袭的抑制能力。结论食管癌中过表达miR-15b可能通过下调LPAR3表达,抑制食管癌细胞增殖、迁移和侵袭。展开更多
Background and Aims:LPAR6 is the most recently deter-mined G protein-coupled receptor of lysophosphatidic acid,and hardly any study has demonstrated the performance of LPAR6 in cancers.We sought to clarify the relatio...Background and Aims:LPAR6 is the most recently deter-mined G protein-coupled receptor of lysophosphatidic acid,and hardly any study has demonstrated the performance of LPAR6 in cancers.We sought to clarify the relationship of LPAR6 to prognosis potential and tumor infiltration im-mune cells in different cancers.Methods:The expression of LPAR6 and its clinical characteristics were evaluated on various databases.The association between LPAR6 and im-mune infiltrates of various types of cancer were investigated via TIMER.Results:We determined that higher LPAR6 ex-pression level was associated with a better overall survival.Additionally,high LPAR6 expression level was significantly associated with better disease-specific survival(DSS)in bladder cancer,and better overall survival(OS)/progres-sion-free survival(PFS)/distant metastasis-free survival(DMFS)/relapse-free survival(RFS)in breast cancer and some other types of cancers.Moreover,LPAR6 significant-ly affects the prognosis of various cancers via The Cancer Genome Atlas(TCGA).Further research exposed that the mRNA level of LPAR6 was positively coordinated with in-filtrating levels of devious immune cells in hepatocellular carcinoma.Conclusions:Our results imply that LPAR6 is associated with prognosis potential and immune infiltration levels in liver cancer.Moreover,LPAR6 expression possibly contributes to the activation of CD8+T,naive T,effector T cells and natural killer cells and inactivates T regulatory cells,decreases T cell exhaustion and regulate T helper cells in liver cancer.These discoveries imply that LPAR6 could be a novel biomarker of prognosis for indicating progno-sis potential and immune-infiltrating level in hepatocellular carcinoma.展开更多
文摘虚拟化技术日益成为关注的焦点,正在最大程度地改变企业 IT 基础设施的部署及运营。甘肃电力协同办公系统打破原有本部、各地市公司两级部署模式,采用大集中部署服务器的技术架构构建电力业务应用系统。 2 台 IBM p6 570 小型机采用逻辑分区技术(LPAR),将单台服务器划分成多个逻辑服务器,彼此运行独立的应用程序。逻辑分区技术在甘肃电力协同办公系统中的应用,不仅可以减少服务器的数量,通过降低空间、散热以及电力消耗等途径提供一种服务器整合的方法,节省成本投资;而且可以提高可用性,带来具有透明负载均衡、动态迁移、故障自动隔离、系统自动重构的高可靠服务器应用环境。
文摘目的探讨miR-15b靶向LPAR3调控食管癌细胞增殖、迁移及侵袭的影响。方法取对数生长期的人食管癌Eca109细胞分为对照组、miR-15b mimic组、miR-NC组、miR-15b mimic+pcDNA3.1组、miR-15b mimic+pcDNA-LPAR3组;另设对数期生长的人食管上皮细胞HEEpiC为空白组。采用qRT-PCR检测各组细胞中miR-15b和LPAR3 mRNA的表达;应用MTT法、划痕实验和Transwell小室实验分别检测各组细胞的增殖、迁移和侵袭情况;Western blot检测Eca109细胞中LPAR3和增殖、迁移、侵袭相关蛋白(Cyclin D1、MMP-2、MMP-9)的表达。结果与空白组相比,对照组Eca109细胞中miR-15b水平明显降低(1.00±0 vs 0.42±0.03,t=33.486,P<0.01),LPAR3 mRNA水平显著升高(1.00±0 vs 2.33±0.15,t=15.358,P<0.01);与对照组相比,miR-15b mimic组细胞miR-15b水平显著升高(0.42±0.03 vs 4.61±0.38,t=19.039,P<0.01),LPAR3 mRNA(2.33±0.15 vs 1.29±0.13)和蛋白(1.12±0.13 vs 0.35±0.03)水平、细胞增殖率(100.00±0 vs 64.57±6.11)、划痕愈合率(63.45±7.20 vs 36.52±4.29)、Eca109细胞进入Transwell小室下层的数量(155.31±14.23 vs 101.33±11.45)、Cyclin D1(0.85±0.10 vs 0.38±0.05)、MMP-2(0.76±0.08 vs 0.33±0.04)、MMP-9(0.94±0.10 vs 0.41±0.05)蛋白表达均显著降低(t=9.075、9.996、10.044、5.565、5.119、7.281、8.327、8.211,P<0.01);使用pcDNA-LPAR3过表达LPAR3能明显逆转miR-15b mimic对细胞增殖、迁移和侵袭的抑制能力。结论食管癌中过表达miR-15b可能通过下调LPAR3表达,抑制食管癌细胞增殖、迁移和侵袭。
基金National Natural Science Foundation of China(81660588,81773582)New Catalytic Process in Clean Energy Production(ZDZX201803)+1 种基金the Key R&D Program of Ningxia(2018BFG02004)the Program for Leading Talents of Ningxia Province(KJT2018004)。
基金This study was supported by grants from the National Natural Science Foundation(81630086,82030099)the National Key R&D Program of China(2018YFC2000700)+3 种基金Shanghai Public Health System Construction Three-Year Action Plan(GWV-10.1-XK15)Innovative research team of high-level local universities in Shanghai for H.W.and the National Science Foundation of China(NSFC81702730)the Start-up Plan for New Young Teacher of SHSMU(KJ30214190026)of JH.
文摘Background and Aims:LPAR6 is the most recently deter-mined G protein-coupled receptor of lysophosphatidic acid,and hardly any study has demonstrated the performance of LPAR6 in cancers.We sought to clarify the relationship of LPAR6 to prognosis potential and tumor infiltration im-mune cells in different cancers.Methods:The expression of LPAR6 and its clinical characteristics were evaluated on various databases.The association between LPAR6 and im-mune infiltrates of various types of cancer were investigated via TIMER.Results:We determined that higher LPAR6 ex-pression level was associated with a better overall survival.Additionally,high LPAR6 expression level was significantly associated with better disease-specific survival(DSS)in bladder cancer,and better overall survival(OS)/progres-sion-free survival(PFS)/distant metastasis-free survival(DMFS)/relapse-free survival(RFS)in breast cancer and some other types of cancers.Moreover,LPAR6 significant-ly affects the prognosis of various cancers via The Cancer Genome Atlas(TCGA).Further research exposed that the mRNA level of LPAR6 was positively coordinated with in-filtrating levels of devious immune cells in hepatocellular carcinoma.Conclusions:Our results imply that LPAR6 is associated with prognosis potential and immune infiltration levels in liver cancer.Moreover,LPAR6 expression possibly contributes to the activation of CD8+T,naive T,effector T cells and natural killer cells and inactivates T regulatory cells,decreases T cell exhaustion and regulate T helper cells in liver cancer.These discoveries imply that LPAR6 could be a novel biomarker of prognosis for indicating progno-sis potential and immune-infiltrating level in hepatocellular carcinoma.
