In this study,Lactobacillus plantarum LP104(LP104)originating from kimchi was treated to C57BL/6N mice fed a chronic-plus-single-binge ethanol diet for ten days,aiming to evaluate the effects of the probiotics on inte...In this study,Lactobacillus plantarum LP104(LP104)originating from kimchi was treated to C57BL/6N mice fed a chronic-plus-single-binge ethanol diet for ten days,aiming to evaluate the effects of the probiotics on intestinal and brain inflammation in the alcohol-induced liver injury model.Dietary supplementation with LP104 significantly reduced the concentrations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in alcoholic hepatic injury mice.The 16S rDNA sequencing results inferred that LP104 modulated the intestinal microbiota by depleting inflammation-related bacterial genera Proteobacteria and enriching Lactobacillus and Roseburia.Furthermore,LP104 intervention attenuated alcohol-induced gut inflammation by the TLR4 signaling pathway,which regulated the expressions of intestinal tight junction proteins such as ZO-1,Claudin-1 and Occludin.Subsequently,LP104 intake exerted neuroprotection by restoring neurotrophic factor levels and the blood-brain barrier(BBB)function to decrease lipopolysaccharide(LPS)levels in the serum.The western blotting results suggested LP104 ameliorated alcohol-induced brain inflammation by inhibiting the LPS/TLR4 signaling pathway in mice.Spearman’s correlation analysis demonstrated that differential intestinal bacterial taxa were connected with the expressions of inflammation-related proteins in both gut and brain of alcohol liver injury mice.展开更多
基金supported by the Jilin Province Science and Technology Plan Project(20230402032 GH).
文摘In this study,Lactobacillus plantarum LP104(LP104)originating from kimchi was treated to C57BL/6N mice fed a chronic-plus-single-binge ethanol diet for ten days,aiming to evaluate the effects of the probiotics on intestinal and brain inflammation in the alcohol-induced liver injury model.Dietary supplementation with LP104 significantly reduced the concentrations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in alcoholic hepatic injury mice.The 16S rDNA sequencing results inferred that LP104 modulated the intestinal microbiota by depleting inflammation-related bacterial genera Proteobacteria and enriching Lactobacillus and Roseburia.Furthermore,LP104 intervention attenuated alcohol-induced gut inflammation by the TLR4 signaling pathway,which regulated the expressions of intestinal tight junction proteins such as ZO-1,Claudin-1 and Occludin.Subsequently,LP104 intake exerted neuroprotection by restoring neurotrophic factor levels and the blood-brain barrier(BBB)function to decrease lipopolysaccharide(LPS)levels in the serum.The western blotting results suggested LP104 ameliorated alcohol-induced brain inflammation by inhibiting the LPS/TLR4 signaling pathway in mice.Spearman’s correlation analysis demonstrated that differential intestinal bacterial taxa were connected with the expressions of inflammation-related proteins in both gut and brain of alcohol liver injury mice.
