摘要
In this study,Lactobacillus plantarum LP104(LP104)originating from kimchi was treated to C57BL/6N mice fed a chronic-plus-single-binge ethanol diet for ten days,aiming to evaluate the effects of the probiotics on intestinal and brain inflammation in the alcohol-induced liver injury model.Dietary supplementation with LP104 significantly reduced the concentrations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(TC),triglycerides(TG)and low-density lipoprotein cholesterol(LDL-C)in alcoholic hepatic injury mice.The 16S rDNA sequencing results inferred that LP104 modulated the intestinal microbiota by depleting inflammation-related bacterial genera Proteobacteria and enriching Lactobacillus and Roseburia.Furthermore,LP104 intervention attenuated alcohol-induced gut inflammation by the TLR4 signaling pathway,which regulated the expressions of intestinal tight junction proteins such as ZO-1,Claudin-1 and Occludin.Subsequently,LP104 intake exerted neuroprotection by restoring neurotrophic factor levels and the blood-brain barrier(BBB)function to decrease lipopolysaccharide(LPS)levels in the serum.The western blotting results suggested LP104 ameliorated alcohol-induced brain inflammation by inhibiting the LPS/TLR4 signaling pathway in mice.Spearman’s correlation analysis demonstrated that differential intestinal bacterial taxa were connected with the expressions of inflammation-related proteins in both gut and brain of alcohol liver injury mice.
基金
supported by the Jilin Province Science and Technology Plan Project(20230402032 GH).
