Lotus(Nelumbo spp.)is a valuable plant resource with promising applications in the cosmetic and food industries.To effectively utilize the aromatic compounds of lotus flowers,it is essential to clarify the optimal har...Lotus(Nelumbo spp.)is a valuable plant resource with promising applications in the cosmetic and food industries.To effectively utilize the aromatic compounds of lotus flowers,it is essential to clarify the optimal harvesting organ and flowering stage.In this study,headspace solidphase microextraction(HS-SPME)and gas chromatography-mass spectrometry(GC—MS)were employed to investigate the volatile profiles of four lotus cultivars across different flowering stages and floral organs.A total of 63 volatile compounds were identified,including 36 terpenes,10 benzenoids/phenylpropanoids,and 17 fatty acid derivatives.Among them,1,4-dimethoxybenzene was the dominant aromatic compound.The volatile profiles of petals differed significantly from those of the other five floral organs.The appendages,a component of the stamens,released the highest concentrations of volatiles,with peak emission at the initial-flowering or full-flowering stages.However,due to pollen dispersal,lotus flowers at full flowering were unsuitable for food or cosmetic applications.Thus,appendages harvested during the initialflowering stage were the most suitable material for high-quality extraction.This study lays a foundation for the industrial development of lotus flower fragrance.展开更多
[Objectives]To investigate the potential applications and mechanisms of action of medicinal plants as bacteriostatic agents,utilizing snow lotus as a case study through network pharmacology.[Methods]The TCMSP and HIT ...[Objectives]To investigate the potential applications and mechanisms of action of medicinal plants as bacteriostatic agents,utilizing snow lotus as a case study through network pharmacology.[Methods]The TCMSP and HIT 2.0 databases were employed to screen and obtain the active components and corresponding targets of snow lotus.The identified targets were subsequently intersected with the antibacterial and bacteriostatic targets sourced from the GeneCards and OMIM databases,resulting in the identification of the antibacterial and bacteriostatic targets associated with snow lotus.Cytoscape software was employed to construct the network diagram illustrating the active components and their corresponding action targets for snow lotus,as well as to analyze the network s topology.Additionally,GO enrichment analysis of the action targets was conducted utilizing the DAVID database.[Results]A total of 12 active components of snow lotus were identified through screening,which corresponded to 294 action targets.Subsequent analysis revealed 117 core action targets of snow lotus that exhibit antibacterial and bacteriostatic properties.The results from the network diagram suggested that snow lotus may exert its antibacterial and bacteriostatic effects through active components such as quercetin,apigenin,and luteolin.Additionally,it appeared to activate the immunomodulatory functions of the human body by interacting with targets such as CASP3,TNF,and IL-6.[Conclusions]Snow lotus may demonstrate antibacterial and bacteriostatic properties through mechanisms of action that involve multiple components,targets,and pathways,from in vivo and in vitro multiple pathways.The integration of botanical bacteriostatic agents with chemical disinfectants that possess broad-spectrum bactericidal effects is advantageous for broadening the bactericidal spectrum and minimizing irritation,in order to facilitate the development of more environmentally friendly and low-toxicity disinfection and bacteriostatic products.展开更多
BACKGROUND Lotus plumule and its active components have demonstrated inhibitory effects on gastric cancer(GC).However,the molecular mechanism of lotus plumule against GC remains unclear and requires further investigat...BACKGROUND Lotus plumule and its active components have demonstrated inhibitory effects on gastric cancer(GC).However,the molecular mechanism of lotus plumule against GC remains unclear and requires further investigation.AIM To identify the key hub genes associated with the anti-GC effects of lotus plumule.METHODS This study investigated the potential targets of traditional Chinese medicine for inhibiting GC using weighted gene co-expression network analysis and bio-informatics.Initially,the active components and targets of the lotus plumule and the differentially expressed genes associated with GC were identified.Sub-sequently,a protein-protein interaction network was constructed to elucidate the interactions between drug targets and disease-related genes,facilitating the identification of hub genes within the network.The clinical significance of these hub genes was evaluated,and their upstream transcription factors and down-stream targets were identified.The binding ability of a hub gene with its down-stream targets was verified using molecular docking technology.Finally,molecular docking was performed to evaluate the binding affinity between the active ingredients of lotus plumule and the hub gene.RESULTS This study identified 26 genes closely associated with GC.Machine learning analysis and external validation narrowed the list to four genes:Aldo-keto reductase family 1 member B10,fructose-bisphosphatase 1,protein arginine methyltransferase 1,and carbonic anhydrase 9.These genes indicated a strong correlation with anti-GC activity.CONCLUSION Lotus plumule exhibits anti-GC effects.This study identified four hub genes with potential as novel targets for diagnosing and treating GC,providing innovative perspectives for its clinical management.展开更多
Non-alcoholic fatty liver disease(NAFLD)is a globally prevalent chronic liver disease with a complex pathogenesis,driven by an intricate interplay of metabolic disorders,oxidative stress,inflammatory,and gut microbiot...Non-alcoholic fatty liver disease(NAFLD)is a globally prevalent chronic liver disease with a complex pathogenesis,driven by an intricate interplay of metabolic disorders,oxidative stress,inflammatory,and gut microbiota imbalance.With changes in lifestyle and dietary habits,in recent years,the incidence of NAFLD has significantly risen,becoming an important public health issue.The traditional Chinese medicine(TCM)diagnostic and therapeutic system has shown unique advantages in the prevention and treatment of NAFLD,especially the use of lotus leaf,which has attracted attention due to its multi-target and multi-pathway regulatory effects.This review examines the pathogenesis of NAFLD,explores the application of TCM in NAFLD,and focuses on the pharmacological effects of lotus leaf and its potential therapeutic value,providing a theoretical basis for the comprehensive prevention and treatment of NAFLD.展开更多
Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived c...Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived carbon by carbonization of one hour displayed excellent capacitive performance benefits from the comprehensive effect of hierarchically porous structure with large SSA,more mesopores,good electrical conductivity and high heteroatom doping.Coin-type ZHSCs deliver 164.4 F·g^(-1)at 0.2 A·g^(-1)and 70.0 F·g^(-1)at 20 A·g^(-1)with capacitance retention of 42.6%assembled with carbonic cathode and Zn@Zn_(3)(PO_(4))_(2)anode using 2 M ZnSO4 solution as electrolyte.Moreover,coin-type ZHSCs deliver the maximum energy density of 65.0 Wh·kg^(-1)at 168.7 W·kg^(-1)and the maximum power density of 11.4 kW·kg^(-1)at 12.7 Wh·kg^(-1).Thanks to the multifunctional Zn_(3)(PO_(4))_(2)interphase as Zn^(2+)-transfer ionic conductor and physical barrier.Moreover,coin-type ZHSCs exhibit outstanding recyclability with capacitance retention of 97.5%and coulombic efficiency of 100%after 10000 charge-discharge cycles at 1 A·g^(-1).展开更多
It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grow...It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active.展开更多
Angiosperms need light to synthesize chlorophyll, but lotus (Nelumbo nucifera Gaertn.) embryo was suspected to have the ability to form chlorophyll in the dark because lotus embryo can turn into green under the covera...Angiosperms need light to synthesize chlorophyll, but lotus (Nelumbo nucifera Gaertn.) embryo was suspected to have the ability to form chlorophyll in the dark because lotus embryo can turn into green under the coverage of four layers of integuments (cotyledon, seed coat, pericarp, lotus pod) which were thought impossible for light to pass through. The authors excluded this possibility based on two experimental results: First, enclosing the young lotus pod with aluminium foil, the growth of louts embryo continued, but the chlorophyll formation was seriously inhibited. A lot of protochlorophyllide, chlorophyll precursor, were accumulated, most of which were combined with LPOR (light dependent protochlorophyllide oxidoreductase). Second, DPOR (dark or light-independent protochlorophyllide oxidoreductase) was the enzyme necessary for chlorophyll synthesis in the dark. The genes encoding DPOR were conservative in many species, but no homologues could be found in lotus genome. Taken together, authers' results clearly demonstrated that lotus embryo synthesizes chlorophyll only through the light-dependent pathway.展开更多
Objective To study the co-effect of procyanidins extracted from the lotus seed pod （LSPC） and bilobalide （BIL） on ameliorating scopolamine-induced learning and memory impairment in young mice. Methods Fifty male K...Objective To study the co-effect of procyanidins extracted from the lotus seed pod （LSPC） and bilobalide （BIL） on ameliorating scopolamine-induced learning and memory impairment in young mice. Methods Fifty male Kunming mice with similar learning and memory capabilities were selected by Morris water maze test and were randomized into 5 groups （n=10 in each group）： control group, scopolamine group, L-（LSPC＋BIL） group （50 mg/kg LSPC＋10 mg/kg BIL）, M-（LSPC＋BIL） group （100 mg/kg LSPC＋20 mg/kg BIL）, H-（LSPC＋BIL） group （150 mg/kg LSPC＋30 mg/kg BIL）. Scopolamine model with impaired learning and memory was established by scopolamine treatment （1 mg/kg）, and after 10 min mice were tested. In L-, M-, and H- （LSPC＋BIL） groups, mice were treated with LSPC and BIL ig. for 30 days, while mice in the other 2 groups were treated with normal saline ig. instead. After the 30-day＇s treatment, the co-effect of LSPC and BIL on learning and memory was tested by Morris water maze and the step-down avoidance tests. Results The memory impairment caused by scopolamine in young mice could be ameliorated by co-treatment of LSPC and BIL, as indicated by significantly shorter escape latency and swimming distance in the Morris water maze test, when compared with those in the scopolamine group. In the step-down avoidance test, mice in all the 3 dose groups showed significantly smaller number of errors and longer latency than mice in the scopolamine group did. Conclusion Co-treatment of LSPE and BIL can ameliorate scopolamine-induced learning and memory impairment in young mice.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant No.32102413)the Biological Resources Programme CAS(Grant No.KFJ-BRP-017-44)Zhongshan Sinno Co.,Ltd.
文摘Lotus(Nelumbo spp.)is a valuable plant resource with promising applications in the cosmetic and food industries.To effectively utilize the aromatic compounds of lotus flowers,it is essential to clarify the optimal harvesting organ and flowering stage.In this study,headspace solidphase microextraction(HS-SPME)and gas chromatography-mass spectrometry(GC—MS)were employed to investigate the volatile profiles of four lotus cultivars across different flowering stages and floral organs.A total of 63 volatile compounds were identified,including 36 terpenes,10 benzenoids/phenylpropanoids,and 17 fatty acid derivatives.Among them,1,4-dimethoxybenzene was the dominant aromatic compound.The volatile profiles of petals differed significantly from those of the other five floral organs.The appendages,a component of the stamens,released the highest concentrations of volatiles,with peak emission at the initial-flowering or full-flowering stages.However,due to pollen dispersal,lotus flowers at full flowering were unsuitable for food or cosmetic applications.Thus,appendages harvested during the initialflowering stage were the most suitable material for high-quality extraction.This study lays a foundation for the industrial development of lotus flower fragrance.
基金Supported by Shanghai Putuo District Science and Technology R&D Platform Project(2024QX04).
文摘[Objectives]To investigate the potential applications and mechanisms of action of medicinal plants as bacteriostatic agents,utilizing snow lotus as a case study through network pharmacology.[Methods]The TCMSP and HIT 2.0 databases were employed to screen and obtain the active components and corresponding targets of snow lotus.The identified targets were subsequently intersected with the antibacterial and bacteriostatic targets sourced from the GeneCards and OMIM databases,resulting in the identification of the antibacterial and bacteriostatic targets associated with snow lotus.Cytoscape software was employed to construct the network diagram illustrating the active components and their corresponding action targets for snow lotus,as well as to analyze the network s topology.Additionally,GO enrichment analysis of the action targets was conducted utilizing the DAVID database.[Results]A total of 12 active components of snow lotus were identified through screening,which corresponded to 294 action targets.Subsequent analysis revealed 117 core action targets of snow lotus that exhibit antibacterial and bacteriostatic properties.The results from the network diagram suggested that snow lotus may exert its antibacterial and bacteriostatic effects through active components such as quercetin,apigenin,and luteolin.Additionally,it appeared to activate the immunomodulatory functions of the human body by interacting with targets such as CASP3,TNF,and IL-6.[Conclusions]Snow lotus may demonstrate antibacterial and bacteriostatic properties through mechanisms of action that involve multiple components,targets,and pathways,from in vivo and in vitro multiple pathways.The integration of botanical bacteriostatic agents with chemical disinfectants that possess broad-spectrum bactericidal effects is advantageous for broadening the bactericidal spectrum and minimizing irritation,in order to facilitate the development of more environmentally friendly and low-toxicity disinfection and bacteriostatic products.
基金Supported by Ningxia Key Research and Development Program,No.2023BEG02015Talent Development Projects of Young Qihuang of National Administration of Traditional Chinese Medicine(2020).
文摘BACKGROUND Lotus plumule and its active components have demonstrated inhibitory effects on gastric cancer(GC).However,the molecular mechanism of lotus plumule against GC remains unclear and requires further investigation.AIM To identify the key hub genes associated with the anti-GC effects of lotus plumule.METHODS This study investigated the potential targets of traditional Chinese medicine for inhibiting GC using weighted gene co-expression network analysis and bio-informatics.Initially,the active components and targets of the lotus plumule and the differentially expressed genes associated with GC were identified.Sub-sequently,a protein-protein interaction network was constructed to elucidate the interactions between drug targets and disease-related genes,facilitating the identification of hub genes within the network.The clinical significance of these hub genes was evaluated,and their upstream transcription factors and down-stream targets were identified.The binding ability of a hub gene with its down-stream targets was verified using molecular docking technology.Finally,molecular docking was performed to evaluate the binding affinity between the active ingredients of lotus plumule and the hub gene.RESULTS This study identified 26 genes closely associated with GC.Machine learning analysis and external validation narrowed the list to four genes:Aldo-keto reductase family 1 member B10,fructose-bisphosphatase 1,protein arginine methyltransferase 1,and carbonic anhydrase 9.These genes indicated a strong correlation with anti-GC activity.CONCLUSION Lotus plumule exhibits anti-GC effects.This study identified four hub genes with potential as novel targets for diagnosing and treating GC,providing innovative perspectives for its clinical management.
基金Sichuan Provincial Administration of Traditional Chinese Medicine Scientific Research Project(Project No.:2023MS250)Chengdu Medical Research Project(Project No.:2021077)。
文摘Non-alcoholic fatty liver disease(NAFLD)is a globally prevalent chronic liver disease with a complex pathogenesis,driven by an intricate interplay of metabolic disorders,oxidative stress,inflammatory,and gut microbiota imbalance.With changes in lifestyle and dietary habits,in recent years,the incidence of NAFLD has significantly risen,becoming an important public health issue.The traditional Chinese medicine(TCM)diagnostic and therapeutic system has shown unique advantages in the prevention and treatment of NAFLD,especially the use of lotus leaf,which has attracted attention due to its multi-target and multi-pathway regulatory effects.This review examines the pathogenesis of NAFLD,explores the application of TCM in NAFLD,and focuses on the pharmacological effects of lotus leaf and its potential therapeutic value,providing a theoretical basis for the comprehensive prevention and treatment of NAFLD.
基金Funded by the Natural Science Foundation of Hubei Province(No.2024AFB930)the Scientific Research Project of Department of Education of Hubei Province(No.B2022096)+2 种基金the Scientific Research Project of Enshi Tujia&Miao Autonomous Prefecture(No.D20220073)the Open Fund of Hubei Key Laboratory of Biological Resources Protection and Utilization(Hubei Minzu University)(No.PT012210)the Hengsheng Industry Donation Fund(No.2025001)。
文摘Carbonaceous cathode materials were prepared by a low-cost and facile molten salt carbonization of lotus stalks in molten carbonates at 850℃for aqueous zinc-ion hybrid supercapacitors(ZHSCs).The lotus stalk-derived carbon by carbonization of one hour displayed excellent capacitive performance benefits from the comprehensive effect of hierarchically porous structure with large SSA,more mesopores,good electrical conductivity and high heteroatom doping.Coin-type ZHSCs deliver 164.4 F·g^(-1)at 0.2 A·g^(-1)and 70.0 F·g^(-1)at 20 A·g^(-1)with capacitance retention of 42.6%assembled with carbonic cathode and Zn@Zn_(3)(PO_(4))_(2)anode using 2 M ZnSO4 solution as electrolyte.Moreover,coin-type ZHSCs deliver the maximum energy density of 65.0 Wh·kg^(-1)at 168.7 W·kg^(-1)and the maximum power density of 11.4 kW·kg^(-1)at 12.7 Wh·kg^(-1).Thanks to the multifunctional Zn_(3)(PO_(4))_(2)interphase as Zn^(2+)-transfer ionic conductor and physical barrier.Moreover,coin-type ZHSCs exhibit outstanding recyclability with capacitance retention of 97.5%and coulombic efficiency of 100%after 10000 charge-discharge cycles at 1 A·g^(-1).
文摘It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active.
文摘Angiosperms need light to synthesize chlorophyll, but lotus (Nelumbo nucifera Gaertn.) embryo was suspected to have the ability to form chlorophyll in the dark because lotus embryo can turn into green under the coverage of four layers of integuments (cotyledon, seed coat, pericarp, lotus pod) which were thought impossible for light to pass through. The authors excluded this possibility based on two experimental results: First, enclosing the young lotus pod with aluminium foil, the growth of louts embryo continued, but the chlorophyll formation was seriously inhibited. A lot of protochlorophyllide, chlorophyll precursor, were accumulated, most of which were combined with LPOR (light dependent protochlorophyllide oxidoreductase). Second, DPOR (dark or light-independent protochlorophyllide oxidoreductase) was the enzyme necessary for chlorophyll synthesis in the dark. The genes encoding DPOR were conservative in many species, but no homologues could be found in lotus genome. Taken together, authers' results clearly demonstrated that lotus embryo synthesizes chlorophyll only through the light-dependent pathway.
基金supported by the National Key Technology R&D Program of China (No.2006BAD27B08 and 2006BAD27B09-4)
文摘Objective To study the co-effect of procyanidins extracted from the lotus seed pod （LSPC） and bilobalide （BIL） on ameliorating scopolamine-induced learning and memory impairment in young mice. Methods Fifty male Kunming mice with similar learning and memory capabilities were selected by Morris water maze test and were randomized into 5 groups （n=10 in each group）： control group, scopolamine group, L-（LSPC＋BIL） group （50 mg/kg LSPC＋10 mg/kg BIL）, M-（LSPC＋BIL） group （100 mg/kg LSPC＋20 mg/kg BIL）, H-（LSPC＋BIL） group （150 mg/kg LSPC＋30 mg/kg BIL）. Scopolamine model with impaired learning and memory was established by scopolamine treatment （1 mg/kg）, and after 10 min mice were tested. In L-, M-, and H- （LSPC＋BIL） groups, mice were treated with LSPC and BIL ig. for 30 days, while mice in the other 2 groups were treated with normal saline ig. instead. After the 30-day＇s treatment, the co-effect of LSPC and BIL on learning and memory was tested by Morris water maze and the step-down avoidance tests. Results The memory impairment caused by scopolamine in young mice could be ameliorated by co-treatment of LSPC and BIL, as indicated by significantly shorter escape latency and swimming distance in the Morris water maze test, when compared with those in the scopolamine group. In the step-down avoidance test, mice in all the 3 dose groups showed significantly smaller number of errors and longer latency than mice in the scopolamine group did. Conclusion Co-treatment of LSPE and BIL can ameliorate scopolamine-induced learning and memory impairment in young mice.
