[Objectives]This study was conducted to efficiently extract loganin from Viburnum erosum Thunb.[Methods]Ultrasound-assisted extraction coupled with response surface methodology was employed.The extraction was carried ...[Objectives]This study was conducted to efficiently extract loganin from Viburnum erosum Thunb.[Methods]Ultrasound-assisted extraction coupled with response surface methodology was employed.The extraction was carried out with 60%methanol as the solvent and loganin extraction rate as the evaluation index.The effects of liquid-to-solid ratio,extraction temperature,and extraction time on loganin extraction rate were investigated through single-factor experiments.Subsequently,a Box-Behnken experimental design was implemented based on the single-factor results to optimize the extraction process.[Results]Under the conditions of liquid-to-solid ratio of 6 ml/g,extraction temperature of 39℃,and extraction time of 88 min,the loganin extraction rate was 4.199%,which agreed with the predicted value of 4.271%.The order of three factors affecting the extraction rate of loganin in V.erosum Thunb was liquid-to-solid ratio>extraction time>extraction temperature.The response surface methodology demonstrates high stability and feasibility for optimizing the extraction process,and the proposed protocol achieves efficient loganin extraction from V.erosum Thunb.[Conclusions]This study provides a novel approach for extracting iridoid glycosides from V.erosum Thunb.and expands the plant sources of loganin.展开更多
Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocyte...Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocytes using lipopolysaccharide(LPS)and subsequently treated these cells with LOG.We employed fluorescence analysis to quantify reactive oxygen species(ROS)levels and measured the expression of NLRP3 and nuclear factor erythropoietin-2-related factor 2(NRF2)using real-time quantitative polymerase chain reaction(qRT-PCR),Western blotting,and immunofluorescence(IF)techniques.Additionally,we developed an OA mouse model by performing medial meniscus destabilization(DMM)surgery and monitored disease progression through micro-com-puted tomography(micro-CT),hematoxylin and eosin(H&E)staining,safranin O and fast green(S&F)staining,and immunohisto-chemical(IHC)analysis.Our results indicate that LOG significantly reduced LPS-induced ROS levels in chondrocytes,inhibited the activation of the NLRP3 inflammasome,and enhanced NRF2/heme oxygenase 1(HO-1)signaling.In vivo,LOG treatment mitigated cartilage degradation and osteophyte formation triggered by DMM surgery,decreased NLRP3 expression,and increased NRF2 expres-sion.These findings suggest that LOG has a protective effect against OA,potentially delaying disease progression by inhibiting the ROS-NLRP3-IL-1β axis and activating the NRF2/HO-1 pathway.展开更多
Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A hig...Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.展开更多
基金Supported by Natural Science Foundation Project of Hubei Province(RZ2300001603)Open Fund of Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province(Hubei Institute for Nationalities)(PT012017).
文摘[Objectives]This study was conducted to efficiently extract loganin from Viburnum erosum Thunb.[Methods]Ultrasound-assisted extraction coupled with response surface methodology was employed.The extraction was carried out with 60%methanol as the solvent and loganin extraction rate as the evaluation index.The effects of liquid-to-solid ratio,extraction temperature,and extraction time on loganin extraction rate were investigated through single-factor experiments.Subsequently,a Box-Behnken experimental design was implemented based on the single-factor results to optimize the extraction process.[Results]Under the conditions of liquid-to-solid ratio of 6 ml/g,extraction temperature of 39℃,and extraction time of 88 min,the loganin extraction rate was 4.199%,which agreed with the predicted value of 4.271%.The order of three factors affecting the extraction rate of loganin in V.erosum Thunb was liquid-to-solid ratio>extraction time>extraction temperature.The response surface methodology demonstrates high stability and feasibility for optimizing the extraction process,and the proposed protocol achieves efficient loganin extraction from V.erosum Thunb.[Conclusions]This study provides a novel approach for extracting iridoid glycosides from V.erosum Thunb.and expands the plant sources of loganin.
基金supported by the National Natural Science Foundation of China(No.82074462)the Major Research Project of Guangzhou University of Chinese Medicine(No.2021xk53)the First Affiliated Hospital of Guangzhou University of Chinese Medicine National Center for Traditional Chinese Medicine Inheritance and Innovation Special Research(No.2022QN02).
文摘Loganin(LOG),a bioactive compound derived from Cornus officinalis Siebold & Zucc,has been understudied in the context of osteoarthritis(OA)treatment.In this study,we induced an inflammatory response in chondrocytes using lipopolysaccharide(LPS)and subsequently treated these cells with LOG.We employed fluorescence analysis to quantify reactive oxygen species(ROS)levels and measured the expression of NLRP3 and nuclear factor erythropoietin-2-related factor 2(NRF2)using real-time quantitative polymerase chain reaction(qRT-PCR),Western blotting,and immunofluorescence(IF)techniques.Additionally,we developed an OA mouse model by performing medial meniscus destabilization(DMM)surgery and monitored disease progression through micro-com-puted tomography(micro-CT),hematoxylin and eosin(H&E)staining,safranin O and fast green(S&F)staining,and immunohisto-chemical(IHC)analysis.Our results indicate that LOG significantly reduced LPS-induced ROS levels in chondrocytes,inhibited the activation of the NLRP3 inflammasome,and enhanced NRF2/heme oxygenase 1(HO-1)signaling.In vivo,LOG treatment mitigated cartilage degradation and osteophyte formation triggered by DMM surgery,decreased NLRP3 expression,and increased NRF2 expres-sion.These findings suggest that LOG has a protective effect against OA,potentially delaying disease progression by inhibiting the ROS-NLRP3-IL-1β axis and activating the NRF2/HO-1 pathway.
基金supported by the Qi Huang Scholars Program of the State Administration of Traditional Chinese Medicine(10400633210005)the National Natural Science Foundation of China (NSFC82174329&NSFC81503540)+1 种基金the Key Drug Development Program (2012ZX09103201-005)the Key Research Project of Beijing University of Chinese Medicine (2020-JYB-ZDGG-029)。
文摘Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.
