为了研究在培养基中添加微量生长因子对菌株冷冻干燥存活率的影响,该研究以植物乳植杆菌LIP-1(Lactiplantibacillus plantarum LIP-1)为研究对象,探究培养基中添加尿嘧啶对菌株冷冻干燥存活率的影响及其作用机制。结果表明与未添加尿嘧...为了研究在培养基中添加微量生长因子对菌株冷冻干燥存活率的影响,该研究以植物乳植杆菌LIP-1(Lactiplantibacillus plantarum LIP-1)为研究对象,探究培养基中添加尿嘧啶对菌株冷冻干燥存活率的影响及其作用机制。结果表明与未添加尿嘧啶的空白对照组相比,在培养基中添加0.05 g/L尿嘧啶能够显著提高植物乳植杆菌LIP-1的活菌数与冻干存活率(P<0.05)。对其作用机制进行探究,发现尿嘧啶的加入能够促使菌株合成更多的尿苷二磷酸(P<0.05),从而使肽聚糖含量显著增加(P<0.05),进而提高细胞壁的稳定性;同时尿嘧啶的加入抑制了乳清酸向尿苷二磷酸的转化,使胞内的乳清酸含量显著升高(P<0.05),菌株利用乳清酸的抗氧化能力减轻了活性氧对细胞膜不饱和脂肪酸的氧化程度,进而减少了细胞膜的损伤程度。结果表明植物乳植杆菌LIP-1通过代谢尿嘧啶减轻了细胞壁与细胞膜受到的冻干损伤,提高了菌株的冻干存活率。研究结果为提高菌株冷冻干燥存活率提供了新的方法和思路。展开更多
We determined the effects of different pre-freezing temperatures (−20℃ and −80℃) on the freeze-drying survival rate and stability during room temperature storage of Lactiplantibacillus plantarum LIP-1. The underlyin...We determined the effects of different pre-freezing temperatures (−20℃ and −80℃) on the freeze-drying survival rate and stability during room temperature storage of Lactiplantibacillus plantarum LIP-1. The underlying mechanisms of survival and stability were also assessed. The freeze-drying survival rate and stability during room temperature storage of Lactiplantibacillus plantarum LIP-1 was significantly higher in the −80℃ than −20℃ group (p <0.05). Regarding the underlying mechanism, compared with the −20℃ group, the −80℃ group had a lower level of intracellular reactive oxygen species, lower degree of oxidation of fatty acids in the cell membrane, high integrity and fluidity of the cell membrane during freeze-drying and room temperature storage, and a significantly higher freeze-drying survival rate and storage stability (p<0.05). This study provides reference data that could help improve the freeze-drying survival rate and stability of probiotics during room temperature storage.展开更多
文摘为了研究在培养基中添加微量生长因子对菌株冷冻干燥存活率的影响,该研究以植物乳植杆菌LIP-1(Lactiplantibacillus plantarum LIP-1)为研究对象,探究培养基中添加尿嘧啶对菌株冷冻干燥存活率的影响及其作用机制。结果表明与未添加尿嘧啶的空白对照组相比,在培养基中添加0.05 g/L尿嘧啶能够显著提高植物乳植杆菌LIP-1的活菌数与冻干存活率(P<0.05)。对其作用机制进行探究,发现尿嘧啶的加入能够促使菌株合成更多的尿苷二磷酸(P<0.05),从而使肽聚糖含量显著增加(P<0.05),进而提高细胞壁的稳定性;同时尿嘧啶的加入抑制了乳清酸向尿苷二磷酸的转化,使胞内的乳清酸含量显著升高(P<0.05),菌株利用乳清酸的抗氧化能力减轻了活性氧对细胞膜不饱和脂肪酸的氧化程度,进而减少了细胞膜的损伤程度。结果表明植物乳植杆菌LIP-1通过代谢尿嘧啶减轻了细胞壁与细胞膜受到的冻干损伤,提高了菌株的冻干存活率。研究结果为提高菌株冷冻干燥存活率提供了新的方法和思路。
基金the National Natural Science Foundation of China[grant numbers 31660456].
文摘We determined the effects of different pre-freezing temperatures (−20℃ and −80℃) on the freeze-drying survival rate and stability during room temperature storage of Lactiplantibacillus plantarum LIP-1. The underlying mechanisms of survival and stability were also assessed. The freeze-drying survival rate and stability during room temperature storage of Lactiplantibacillus plantarum LIP-1 was significantly higher in the −80℃ than −20℃ group (p <0.05). Regarding the underlying mechanism, compared with the −20℃ group, the −80℃ group had a lower level of intracellular reactive oxygen species, lower degree of oxidation of fatty acids in the cell membrane, high integrity and fluidity of the cell membrane during freeze-drying and room temperature storage, and a significantly higher freeze-drying survival rate and storage stability (p<0.05). This study provides reference data that could help improve the freeze-drying survival rate and stability of probiotics during room temperature storage.
