摘要
目的探讨过敏性哮喘的治疗方法。方法选取25只雄性BALB/c鼠,随机分为CON、OVA、EXO、LIP-1、LIP-1+EXO组,每组各5只。CON组采用每天PBS雾化吸入30 min;其他各组成功构建哮喘小鼠模型后给予不同的治疗方法。治疗结束后取小鼠肺组织和BALF。利用ELISA法检测BALF中的IgE、IL-4,并进行白细胞总数和细胞分类计数。肺组织切片做HE染色。结果与其他各模型组相比,LIP-1+EXO组可明显降低IL-4、IgE的水平及白细胞总数和各类细胞数量(P<0.05);肺组织炎症细胞浸润减少,气道上皮增生不明显。结论LIP-1+EXO联合能有效治疗过敏性哮喘模型小鼠。
Objective Explore the treatment methods for allergic asthma.Methods 25 male BALB/c mice were randomly divided into CON,OVA,EXO,LIP-1,and LIP-1+EXO groups,with 5 mice in each group.The CON group received daily PBS nebulization inhalation for 30 minutes;After successfully constructing asthma mouse models in other groups,different treatment methods were given.After treatment,lung tissue and BALF were collected from mice.Use ELISA method to detect IgE and IL-4 in BALF,and perform total white blood cell count and cell classification counting.Perform HE staining on lung tissue slices.Results Compared with other model groups,the LIP-1+EXO group significantly reduced the levels of IL-4 and IgE,as well as the total number of white blood cells and various types of cells(P<0.05);The infiltration of inflammatory cells in lung tissue is reduced,and the proliferation of airway epithelium is not significant.Conclusion The combination of LIP-1 and EXO can effectively treat allergic asthma model mice.
作者
殷勇
丁勇
曹琦
张艳丽
马春玲
YIN Yong;DING Yong;CAO Qi;ZHANG Yan-li;MA Chun-ling(Binzhou Medical College,Yantai,China 264003;Linyi City Maternal and Child Health Hospital,Linyi,China 276000)
出处
《山东医学高等专科学校学报》
2024年第6期19-22,F0003,共5页
Journal of Shandong Medical College
