The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental sta...The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental stages,the plants produce leaves that curve inward to produce the leafy head.Many microRNAs and their target genes have been described participating in leaf development and leaf curvature.The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage.We compared the mi RNA and gene abundances between emerging rosette and heading leaves.To remove transcripts(miRNAs and genes)whose regulation was most likely associated with plant age rather than the change from rosette to heading stage,we utilized a non-heading collard green(B.oleracea var.acephala)morphotype as control.This resulted in 33 DEMs and 1998 DEGs with likely roles in the transition from rosette to heading stage in cabbage.Among these 1998 DEGs,we found enriched GO terms related to DNA-binding transcription factor activity,transcription regulator activity,iron ion binding,and photosynthesis.We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed(1998DEGs)between rosette and heading stage leaves to construct mi RNA-target gene interaction networks.Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3(GRF3)and 4(GRF4)in pointed cabbage head formation.展开更多
The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of...The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of the aqueous extract of the recipe based on three leafy vegetables (Amaranthus hybridus L., Spinacia oleracea, Brassica campestris L.) in laboratory rodents. The aqueous extract of the food recipe was administered orally using a cannula. Regarding acute toxicity, the administration of single doses of 5000 and 10,000 mg/kg to mice had no significant effects on behavior. On the other hand, weight loss was significantly (**p < 0.01) compared to control mice (1 mL/100g). The results on subacute toxicity showed that daily administration of the aqueous extract of the food recipe at a dose of 1000 mg/kg for 21 days in rats resulted in a non-significant weight gain, and caused changes in some biochemical parameters including HDL-cholesterol levels, blood sugar levels and ALAT activity.展开更多
The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was ...The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.展开更多
基金funded by the Mexican government through the Consejo Nacional de Ciencia y Tecnología (CONACYT),C.V.761325,for the PhD project of Jorge Aleman-Baez。
文摘The vegetative development of cabbage(Brassica oleracea var.capitata)passes through seedling,rosette,folding and heading stages.Leaves that form the rosette are large and mostly flat.In the following developmental stages,the plants produce leaves that curve inward to produce the leafy head.Many microRNAs and their target genes have been described participating in leaf development and leaf curvature.The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage.We compared the mi RNA and gene abundances between emerging rosette and heading leaves.To remove transcripts(miRNAs and genes)whose regulation was most likely associated with plant age rather than the change from rosette to heading stage,we utilized a non-heading collard green(B.oleracea var.acephala)morphotype as control.This resulted in 33 DEMs and 1998 DEGs with likely roles in the transition from rosette to heading stage in cabbage.Among these 1998 DEGs,we found enriched GO terms related to DNA-binding transcription factor activity,transcription regulator activity,iron ion binding,and photosynthesis.We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed(1998DEGs)between rosette and heading stage leaves to construct mi RNA-target gene interaction networks.Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3(GRF3)and 4(GRF4)in pointed cabbage head formation.
文摘The consumption of a mixture of leafy vegetables has always been a practice anchored in the dietary habits of the people of Brazzaville. The objective of this study was to evaluate the acute and subacute toxicities of the aqueous extract of the recipe based on three leafy vegetables (Amaranthus hybridus L., Spinacia oleracea, Brassica campestris L.) in laboratory rodents. The aqueous extract of the food recipe was administered orally using a cannula. Regarding acute toxicity, the administration of single doses of 5000 and 10,000 mg/kg to mice had no significant effects on behavior. On the other hand, weight loss was significantly (**p < 0.01) compared to control mice (1 mL/100g). The results on subacute toxicity showed that daily administration of the aqueous extract of the food recipe at a dose of 1000 mg/kg for 21 days in rats resulted in a non-significant weight gain, and caused changes in some biochemical parameters including HDL-cholesterol levels, blood sugar levels and ALAT activity.
文摘The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.
