摘要
目的:研究LEAFY基因在巨峰葡萄花芽分化期间的表达差异,为实际生产提供理论依据。方法:用半定量RT-PCR方法研究LEAFY基因在巨峰葡萄花芽分化期间的表达。结果:LEAFY基因的表达量在所研究的8个时期内有明显差异,在5叶期、6叶期、7叶期、8叶期的表达量相对较高。结论:根据成花基因LEAFY表达上的差异,可以人为地提前或延迟巨峰葡萄的花期。
Objective：To provide theoretical basis for the practical production by the study on LEAFY gene expression during the bud differentiation of ‘Kyoho’ grapevine.Methods：The LEAFY genes expression was studied during the period of bud differentiation with the method of semi-quantitative RT-PCR.Results：The LEAFY gene expression showed significant difference in the eight periods,the expression levels in five-leaf stage,six-leaf stage,seven-leaf stage and eight-leaf stage were higher than other stages.Conclusion：According to the differences of flower gene expression,we can advance or delay the flowering of ‘Kyoho’ grapevine.
出处
《生物技术通讯》
CAS
2011年第1期41-44,共4页
Letters in Biotechnology
基金
广西农业科学院发展基金(2007015)
广西农业科学院基本业务项目(200931基)
广西作物遗传生物技术重点开放实验室分子育种技术平台创建项目开放课题(桂科能0815011-6-1-12)
