This paper introduces an instrument and method for lamp life test. The design of the instrument is divided into three steps： the oyerall design scheme, hardware circuit design and host machine programming. The instru...This paper introduces an instrument and method for lamp life test. The design of the instrument is divided into three steps： the oyerall design scheme, hardware circuit design and host machine programming. The instrument contains luminance and temperature sensors which can measure luminance of the lamp lighting and environment temperature in the instrument body. Then, the measure data can be transmitted to PC via the wireless transceiver modules for real-time monitoring. The luminance degradation curve based on the measure data is used to reckon the life of the lamp. In the end, the method for processing measurement data is given.展开更多
Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control m...Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.展开更多
基金Sponsored by the Higher School Scientific Research Project Plan of Department of Education of Liaoning Province of China (Grant No. 2009A085)
文摘This paper introduces an instrument and method for lamp life test. The design of the instrument is divided into three steps： the oyerall design scheme, hardware circuit design and host machine programming. The instrument contains luminance and temperature sensors which can measure luminance of the lamp lighting and environment temperature in the instrument body. Then, the measure data can be transmitted to PC via the wireless transceiver modules for real-time monitoring. The luminance degradation curve based on the measure data is used to reckon the life of the lamp. In the end, the method for processing measurement data is given.
文摘Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.
