目的对银杏叶提取物(extract of Ginkgo biloba L.,EGb)中化学成分及其入血成分进行定性分析。方法采用超高效液相色谱串联四极杆静电场轨道阱质谱方法,在正离子、负离子模式下分析EGb样品溶液、对照品溶液,获得化合物一级、二级质谱信...目的对银杏叶提取物(extract of Ginkgo biloba L.,EGb)中化学成分及其入血成分进行定性分析。方法采用超高效液相色谱串联四极杆静电场轨道阱质谱方法,在正离子、负离子模式下分析EGb样品溶液、对照品溶液,获得化合物一级、二级质谱信息,通过分析典型化合物的裂解规律,结合对照品、文献的质谱信息,定性鉴定EGb中化合物。将20只C57BL/6小鼠随机分为空白对照组与给药组,给药组每日灌胃给予EGb(100 mg/kg),连续给药1周,空白对照组给予等体积空白溶剂。末次给药2小时后处死小鼠并收集血清进行成分分析。结果在EGb中共鉴定出75种化合物,总结其中典型成分银杏内酯B、白果内酯、异槲皮苷的裂解规律;对比EGb、空白血清、含药血清的质谱数据,发现EGb中13种成分以原型进入小鼠血液内。结论具有高分辨率、高灵敏度的超高效液相色谱串联四极杆静电场轨道阱质谱方法适用于EGb及其含药血清的成分分析,为阐释EGb药效物质基础研究提供科学依据。展开更多
[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw mate...[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.展开更多
文摘[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.
