目的探讨三乌胶丸治疗寒湿痹阻证膝骨关节炎的疗效。方法按照随机数表法将304例膝骨关节炎患者分为对照组和研究组,各152例。对照组给予美洛昔康片、硫酸氨基葡萄糖片治疗,研究组在对照组基础上加用三乌胶丸治疗,均治疗28 d。比较2组治...目的探讨三乌胶丸治疗寒湿痹阻证膝骨关节炎的疗效。方法按照随机数表法将304例膝骨关节炎患者分为对照组和研究组,各152例。对照组给予美洛昔康片、硫酸氨基葡萄糖片治疗,研究组在对照组基础上加用三乌胶丸治疗,均治疗28 d。比较2组治疗28 d后的疗效,治疗前和治疗28 d后的中医症状、膝关节功能、疼痛情况、骨代谢指标、血清基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)、基质金属蛋白酶-3(MMP-3)、Smad-1水平及治疗期间的安全性。结果研究组治疗28 d后的总有效率(93.42%,142/152)高于对照组(78.95%,120/152)(P<0.05)。治疗28 d后,2组中医症状、西安大略麦克马斯特大学骨关节炎指数(Western Ontario and McMaster University Osteoarthritis Index,WOAMC)、视觉模拟评分法(visual analog scale,VAS)评分及血清MMP-1、MMP-3水平与治疗前比较降低,研究组低于对照组(P<0.05);血清骨特异性碱性磷酸酶(bone-specifi c alkaline phosphatase,BALP)、骨保护素(osteoprotegerin,OPG)、骨钙素(bone gla-protein,BGP)、Smad-1水平升高,研究组高于对照组(P<0.05)。研究组治疗期间不良反应总发生率低于对照组(P<0.05)。结论三乌胶丸可调节寒湿痹阻证膝骨关节炎患者血清MMP-1、MMP-3、Smad-1及骨代谢指标水平,促进软骨修复,改善患者中医症状、膝关节功能,减轻疼痛程度,提高疗效和安全性。展开更多
AIM: To study the characteristics of the four bioactive pure polysaccharides of Panax quiquefolium L. (PPQI 1～4). METHODS: The polysaccharides were extracted with hot water and grade precipitated with ethanol. They w...AIM: To study the characteristics of the four bioactive pure polysaccharides of Panax quiquefolium L. (PPQI 1～4). METHODS: The polysaccharides were extracted with hot water and grade precipitated with ethanol. They were further purified with Sephadex DEAE A50 and Sephadex G200 chromatography. The molecular weights of the polysaccharides were determined with matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). The sugar compositions were analyzed with GC. The linkage positions of the component sugars were determined by methylation and GC MS. RESULTS: The four polysaccharides are all uronic acid containing heteropolysaccharides with the component sugars of arabinose, galactose and glucose in different molar ratios. The uronic acid contents are within 30%～60%. Their molecular weights are in the range of 2×10 4～7×10 4 u. CONCLUSION: The four new bioactive polysaccharides are all uronic acid containing heteropolysaccharides.展开更多
目的探讨血管紧张素Ⅱ(A ngⅡ)、转化生长因子1β(TGF-1β)、基质金属蛋白酶-1(MM P-1)及其抑制剂(T IM P-1)与原发性高血压(EH)左心室肥厚(LVH)的关系。方法对81例EH患者行超声心动仪检测,根据其结果计算左心室重量指数(LVM I),分为左...目的探讨血管紧张素Ⅱ(A ngⅡ)、转化生长因子1β(TGF-1β)、基质金属蛋白酶-1(MM P-1)及其抑制剂(T IM P-1)与原发性高血压(EH)左心室肥厚(LVH)的关系。方法对81例EH患者行超声心动仪检测,根据其结果计算左心室重量指数(LVM I),分为左心室肥厚(LVH)组和非肥厚(NLVH)组;检测其血中A ngⅡ、TGF-1β、MM P-1、T IM P-1水平,并与对照组进行对比分析。结果EH患者血中A ngⅡ、TGF-1β、T IM P-1水平均较对照组显著增高,而MM P-1水平显著降低;LVH组尤甚。LVM I与A ngⅡ、TGF-1β、T IM P-1呈显著正相关,与MM P-1呈显著负相关。结论MM P-1水平降低、T IM P-1水平升高是导致心肌胶原代谢紊乱,促进LVH形成的重要因素;TGF-1β不仅直接抑制MM P-1表达,而且介导A ngⅡ作用,共同促进LVH形成。展开更多
文摘目的探讨三乌胶丸治疗寒湿痹阻证膝骨关节炎的疗效。方法按照随机数表法将304例膝骨关节炎患者分为对照组和研究组,各152例。对照组给予美洛昔康片、硫酸氨基葡萄糖片治疗,研究组在对照组基础上加用三乌胶丸治疗,均治疗28 d。比较2组治疗28 d后的疗效,治疗前和治疗28 d后的中医症状、膝关节功能、疼痛情况、骨代谢指标、血清基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)、基质金属蛋白酶-3(MMP-3)、Smad-1水平及治疗期间的安全性。结果研究组治疗28 d后的总有效率(93.42%,142/152)高于对照组(78.95%,120/152)(P<0.05)。治疗28 d后,2组中医症状、西安大略麦克马斯特大学骨关节炎指数(Western Ontario and McMaster University Osteoarthritis Index,WOAMC)、视觉模拟评分法(visual analog scale,VAS)评分及血清MMP-1、MMP-3水平与治疗前比较降低,研究组低于对照组(P<0.05);血清骨特异性碱性磷酸酶(bone-specifi c alkaline phosphatase,BALP)、骨保护素(osteoprotegerin,OPG)、骨钙素(bone gla-protein,BGP)、Smad-1水平升高,研究组高于对照组(P<0.05)。研究组治疗期间不良反应总发生率低于对照组(P<0.05)。结论三乌胶丸可调节寒湿痹阻证膝骨关节炎患者血清MMP-1、MMP-3、Smad-1及骨代谢指标水平,促进软骨修复,改善患者中医症状、膝关节功能,减轻疼痛程度,提高疗效和安全性。
文摘AIM: To study the characteristics of the four bioactive pure polysaccharides of Panax quiquefolium L. (PPQI 1～4). METHODS: The polysaccharides were extracted with hot water and grade precipitated with ethanol. They were further purified with Sephadex DEAE A50 and Sephadex G200 chromatography. The molecular weights of the polysaccharides were determined with matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). The sugar compositions were analyzed with GC. The linkage positions of the component sugars were determined by methylation and GC MS. RESULTS: The four polysaccharides are all uronic acid containing heteropolysaccharides with the component sugars of arabinose, galactose and glucose in different molar ratios. The uronic acid contents are within 30%～60%. Their molecular weights are in the range of 2×10 4～7×10 4 u. CONCLUSION: The four new bioactive polysaccharides are all uronic acid containing heteropolysaccharides.
文摘目的探讨血管紧张素Ⅱ(A ngⅡ)、转化生长因子1β(TGF-1β)、基质金属蛋白酶-1(MM P-1)及其抑制剂(T IM P-1)与原发性高血压(EH)左心室肥厚(LVH)的关系。方法对81例EH患者行超声心动仪检测,根据其结果计算左心室重量指数(LVM I),分为左心室肥厚(LVH)组和非肥厚(NLVH)组;检测其血中A ngⅡ、TGF-1β、MM P-1、T IM P-1水平,并与对照组进行对比分析。结果EH患者血中A ngⅡ、TGF-1β、T IM P-1水平均较对照组显著增高,而MM P-1水平显著降低;LVH组尤甚。LVM I与A ngⅡ、TGF-1β、T IM P-1呈显著正相关,与MM P-1呈显著负相关。结论MM P-1水平降低、T IM P-1水平升高是导致心肌胶原代谢紊乱,促进LVH形成的重要因素;TGF-1β不仅直接抑制MM P-1表达,而且介导A ngⅡ作用,共同促进LVH形成。
