Mn(L-Phe)2稨2O]n (Phe=phenylalanine) with a single stranded coordination poly- mer has been synthesized by self-assembly of manganese salt and phenylalanine and characterized by X-ray single-crystal diffraction method...Mn(L-Phe)2稨2O]n (Phe=phenylalanine) with a single stranded coordination poly- mer has been synthesized by self-assembly of manganese salt and phenylalanine and characterized by X-ray single-crystal diffraction method. The complex crystallizes in the orthorhombic, space group P212121 with a = 6.0911(1), b = 10.3642(1), c = 30.4321(7) ? V = 1921.16(6) ?, Z = 4, Dc = 1.387 g/cm3, C18H22N2O5Mn, Mr = 401.32, F(000) = 836 and m(MoKa) = 0.717 mm-1. The final R = 0.0614 and wR = 0.1412 for 2291 observed reflections with I > 2s(I). The magnetic behavior study shows that there exist super-exchange antiferromagnetic interactions between the magnetic centers through the effective exchange pathway of the carboxylate group.展开更多
Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the devel...Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.展开更多
从土壤中筛选出1株具有L-苯丙氨酸解氨酶(L-Pheny la lan ine amm on la-lyase,PAL)较高酶活力的产生菌株——酵母菌SA 1。L-酪氨酸(L-T yr)为该菌株产PAL的最佳诱导物;利用反式肉桂酸转化L-苯丙氨酸(L-Phe)的最佳酶促反应条件为:2.0%...从土壤中筛选出1株具有L-苯丙氨酸解氨酶(L-Pheny la lan ine amm on la-lyase,PAL)较高酶活力的产生菌株——酵母菌SA 1。L-酪氨酸(L-T yr)为该菌株产PAL的最佳诱导物;利用反式肉桂酸转化L-苯丙氨酸(L-Phe)的最佳酶促反应条件为:2.0%反式肉桂酸,碳酸氢铵∶氨水(W∶V)=4∶4,pH 10.6,34°C,反应8 h,在转化体系中添加甘油对酶活力有较好的保护作用。展开更多
基金This work was supported by the grants of the National Natural Science Foundation of China the Chinese Academy of Sciences and Hong Kong Polytechnic University (29901005)
文摘Mn(L-Phe)2稨2O]n (Phe=phenylalanine) with a single stranded coordination poly- mer has been synthesized by self-assembly of manganese salt and phenylalanine and characterized by X-ray single-crystal diffraction method. The complex crystallizes in the orthorhombic, space group P212121 with a = 6.0911(1), b = 10.3642(1), c = 30.4321(7) ? V = 1921.16(6) ?, Z = 4, Dc = 1.387 g/cm3, C18H22N2O5Mn, Mr = 401.32, F(000) = 836 and m(MoKa) = 0.717 mm-1. The final R = 0.0614 and wR = 0.1412 for 2291 observed reflections with I > 2s(I). The magnetic behavior study shows that there exist super-exchange antiferromagnetic interactions between the magnetic centers through the effective exchange pathway of the carboxylate group.
文摘Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.
文摘从土壤中筛选出1株具有L-苯丙氨酸解氨酶(L-Pheny la lan ine amm on la-lyase,PAL)较高酶活力的产生菌株——酵母菌SA 1。L-酪氨酸(L-T yr)为该菌株产PAL的最佳诱导物;利用反式肉桂酸转化L-苯丙氨酸(L-Phe)的最佳酶促反应条件为:2.0%反式肉桂酸,碳酸氢铵∶氨水(W∶V)=4∶4,pH 10.6,34°C,反应8 h,在转化体系中添加甘油对酶活力有较好的保护作用。
