In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-greenA chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyz...In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-greenA chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were sereened out from 20 primers and 47 DNA fragments were ampli ed from 39individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and thepolymorphic rate was 87.23%,which could re ect the genetic variation that was with four plots of each kind and to the individuals.Chuster results showed that at rst,the grey A chinense of Wulimu and the grey-green A chinense of Wulimu were grouped together,and then they grouped with the grey A chinense of green grassland into one chuster,at last with the grey-green A.chinense of greengrassland.A.chinense of identical symusiologic type was grouped together.According to Shannon index,the green grassland of thegrey A chinense had the largest variation degree,which indicated that A.chinense had vaniation among species and interspecies byISSR analysis,but A.chinense didn't differentiate different species.展开更多
The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were iden...The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were identified representing 97.5% of the total components detected. The major constituents of the oil from the samples obtained in the eastern of Riyue Mountain (2700 - 3200 m) were 2-ethenyl-naphthalene (45.1%), beta-pinene (11.2%), 3-carene (8.7%), 3,7-dimethyl-1,3,6- octatriene (7.9%), limonene (5.4%), alpha-pinene (3.5%) and beta-myrcene (2.0%). Whereas the oil from the plant collected in Qilian Mountain (3300 - 3500 m) was composed mainly of thujone (21.4%), 1,8-cineole (18.9%), camphor (9.1%), 4-methyl-1-(1-methyl ethyl)-3-cyclo hexen-1-ol (7.8%), 4-methyl-1-(1-methylethyl)-bicyclo[3.1.0]hexan-3-one (5.3%) and 2-isopropyl-5-methyl- 3-cyclohexen-1-one(5.0%).展开更多
Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen...Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen plant pathogenic fungi were above 50%,which showed the essential oil had broad-spectrum antifungal activity.According to the toxicity curve of essential oil to Alternaria humicola,the EC50 to mycelium growth and spores germination were 326.86 μg/mL and 406.24 μg/mL respectively.Furthermore,the effect of essential oil on cell membrane potential of A.humicola was tested by the conductivity method.The results showed the action of essential oil was changing osmosis of cell membrane of fungi.展开更多
文摘In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-greenA chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were sereened out from 20 primers and 47 DNA fragments were ampli ed from 39individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and thepolymorphic rate was 87.23%,which could re ect the genetic variation that was with four plots of each kind and to the individuals.Chuster results showed that at rst,the grey A chinense of Wulimu and the grey-green A chinense of Wulimu were grouped together,and then they grouped with the grey A chinense of green grassland into one chuster,at last with the grey-green A.chinense of greengrassland.A.chinense of identical symusiologic type was grouped together.According to Shannon index,the green grassland of thegrey A chinense had the largest variation degree,which indicated that A.chinense had vaniation among species and interspecies byISSR analysis,but A.chinense didn't differentiate different species.
文摘The oils extracted by hydro distillation from the aerial parts of Artemisia scoparia waldst. & kitag growing wild in two regions on Qinghai-Tibetan Plateau were analyzed by GC-MS. Eighty-three components were identified representing 97.5% of the total components detected. The major constituents of the oil from the samples obtained in the eastern of Riyue Mountain (2700 - 3200 m) were 2-ethenyl-naphthalene (45.1%), beta-pinene (11.2%), 3-carene (8.7%), 3,7-dimethyl-1,3,6- octatriene (7.9%), limonene (5.4%), alpha-pinene (3.5%) and beta-myrcene (2.0%). Whereas the oil from the plant collected in Qilian Mountain (3300 - 3500 m) was composed mainly of thujone (21.4%), 1,8-cineole (18.9%), camphor (9.1%), 4-methyl-1-(1-methyl ethyl)-3-cyclo hexen-1-ol (7.8%), 4-methyl-1-(1-methylethyl)-bicyclo[3.1.0]hexan-3-one (5.3%) and 2-isopropyl-5-methyl- 3-cyclohexen-1-one(5.0%).
文摘Effect of essential oil from Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag on hypha growth of sixteen plant pathogenic fungi was tested at concentration of 500 μg/mL.The inhibition rates to fourteen plant pathogenic fungi were above 50%,which showed the essential oil had broad-spectrum antifungal activity.According to the toxicity curve of essential oil to Alternaria humicola,the EC50 to mycelium growth and spores germination were 326.86 μg/mL and 406.24 μg/mL respectively.Furthermore,the effect of essential oil on cell membrane potential of A.humicola was tested by the conductivity method.The results showed the action of essential oil was changing osmosis of cell membrane of fungi.
