The 5＇-flanking proximal region of stress-induced gene encoding choline monooxygenase （CMO） was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtai...The 5＇-flanking proximal region of stress-induced gene encoding choline monooxygenase （CMO） was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtained. The transcription start site, which is located at 128 bp upstream to the start ATG, was predicted by the TSSP-TCM program. The functional elements were analysed by PLACE program. The obtained SICMO gene promoter contains the basic elements： TATA-box, CAAT-box, and stress-induced elements, for example, salt responsive element （GAAAAA）, cold responsive elements （CANNTG）, ABA （Abscisic Acid） responsive elements （NAACAA）, water stress element （CGGTTG）, and WUN responsive elements （GTTAGGTTC）. Isolation and analysis of the promoter of the CMO gene from S. liaotungensis lays a foundation for characterising the stress-induced promoter elements, studying the relationship between the structure and function of the promoter, and investigating the molecular mechanism of CMO gene regulation.展开更多
从沉迷阅读的小“书虫”到全球畅销作家,J.K.罗琳用魔法书写传奇,诠释灵感与坚持的力量。J.K.Rowling,born Joanne Rowling in 1965,England,loved books from a young age.She wrote her first story,Rabbit,at six and a novel about ...从沉迷阅读的小“书虫”到全球畅销作家,J.K.罗琳用魔法书写传奇,诠释灵感与坚持的力量。J.K.Rowling,born Joanne Rowling in 1965,England,loved books from a young age.She wrote her first story,Rabbit,at six and a novel about cursed(被诅咒)diamonds at eleven.As a child,she was a bookworm with freckles(雀斑)and glasses.展开更多
基金This work was supported by the National Natural Sciences Foundation of China (No. 30370806).
文摘The 5＇-flanking proximal region of stress-induced gene encoding choline monooxygenase （CMO） was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis K. A total of 2,204 bp DNA sequence was obtained. The transcription start site, which is located at 128 bp upstream to the start ATG, was predicted by the TSSP-TCM program. The functional elements were analysed by PLACE program. The obtained SICMO gene promoter contains the basic elements： TATA-box, CAAT-box, and stress-induced elements, for example, salt responsive element （GAAAAA）, cold responsive elements （CANNTG）, ABA （Abscisic Acid） responsive elements （NAACAA）, water stress element （CGGTTG）, and WUN responsive elements （GTTAGGTTC）. Isolation and analysis of the promoter of the CMO gene from S. liaotungensis lays a foundation for characterising the stress-induced promoter elements, studying the relationship between the structure and function of the promoter, and investigating the molecular mechanism of CMO gene regulation.
文摘从沉迷阅读的小“书虫”到全球畅销作家,J.K.罗琳用魔法书写传奇,诠释灵感与坚持的力量。J.K.Rowling,born Joanne Rowling in 1965,England,loved books from a young age.She wrote her first story,Rabbit,at six and a novel about cursed(被诅咒)diamonds at eleven.As a child,she was a bookworm with freckles(雀斑)and glasses.
