This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabe...This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabetes(T2DM). Random amplified polymorphic DNA, sequence-characterized amplified region, and high-performance liquid chromatography(HPLC) were used for the quality control of Ic-17-7fb. The pharmacological effects on streptozocin(STZ)-induced high fat diet(HFD)-fed Albino Wistar rats were evaluated. The rats underwent the following treatments: control, metformin, Ic-17-7fb(0.166 and 0.5 g·kg;) or without treatment. The fasting blood glucose(FBG), blood glucose, total cholesterol(TC), triglyceride(TG), high-density lipoprotein(HDL-c), and low-density lipoprotein(LDL-c) were measured. Ic-17-7fb amplified a single specific band by S11-2-F3 and S11-2-R3 primers. An HPLC-based quality and quantity method was established for industrial application. The contents of adenosine and N;-(2-hydroxyethyl) adenosine(HEA) of the cultivated Ic-17-7fb were analyzed. All of the validation lots of cultured Ic-17-7fb passed the quantity control of the training set(0.90 mg·g;of adenosine and 0.89 mg·g;of HEA). After two weeks of administration, the average FBG was 4.89 ± 0.42(control), 26.10 ± 5.77(model), 23.63 ± 6.15(metformin), 17.96 ± 9.36(Ic-17-7fb for 0.166 g·kg;), and 19.69 ±8.71 mmol·L;(Ic-17-7fb for 0.5 g·kg;). The FBG of Ic-17-7fb(0.166 g·kg;) treatment significantly reduced by 31.19%, compared with the model after two weeks of administration(P < 0.01). Metformin, Ic-17-7fb(0.166 g·kg;), and Ic-17-7fb(0.5 g·kg;) reduced TC, TG, HDL-c, and LDL-c compared with the T2DM model treatment at the 6 th week of treatment(P < 0.05). This study established the first quality standard for Ic-17-7fb, which can be effectively applied in the treatment of T2DM. The reliable quality control method and pharmacological effect will broaden its application space.展开更多
The influence of twelve different pesticides(six insecticides and six fungicides) on lipase production by Isaria fumosorosea was investigated under laboratory conditions. Imidacloprid caused higher reduction in enzyme...The influence of twelve different pesticides(six insecticides and six fungicides) on lipase production by Isaria fumosorosea was investigated under laboratory conditions. Imidacloprid caused higher reduction in enzyme activities at all three concentrations whereas very low reduction in enzyme activities was caused by azadrichtin and Indoxacarb when used at 10μg/ml. In case of fungicides, propiconazole and pyraclostrobin caused higher reduction in enzyme activities at all three concentrations whereas low reduction in enzyme activities was caused by chlorothalonil and mancozeb when used at 10 μg/ml. Different insecticides significantly affected the toxicity of I. fumosorosea lipase against second instar Dysmicoccus neobrevipesnymphs. I.fumosorosea lipase combined with azadrichtin was proved to be the most aggressive, and those from control were the least virulent with a mean ST50 value of 6.34 ± 0.41 d.展开更多
The rice planthopper,Sogatella furcifera,is a piercing-sucking insect pest of rice,Oryza sativa.It is responsible for significant crop yield losses,and has developed moderate to high resistance to several commonly use...The rice planthopper,Sogatella furcifera,is a piercing-sucking insect pest of rice,Oryza sativa.It is responsible for significant crop yield losses,and has developed moderate to high resistance to several commonly used chemical insecticides.We investigated the effects of the insect fungal pathogen Isaria javanica,alone and in combination with the chemical insecticide dinotefuran,on S.furcifera under both laboratory and field conditions.Our results show that I.javanica displays high infection efficiency and mortality for different stages of S.furcifera,reducing adult survival,female oviposition and ovary development.Laboratory bioassays showed that the combined use of I.javanica with a low dose(4-16 mg L^(-1))of dinotefuran resulted in higher mortality in S.furcifera than the use of I.javanica or dinotefuran alone.The combined treatment also had more significant effects on several host enzymes,including superoxide dismutase,catalase,peroxidase,and prophenol oxidase activities.In field trials,I.javanica effectively suppressed populations of rice planthoppers to low levels(22-64%of the level in untreated plots).Additional field experiments showed synergistic effects,i.e.,enhanced efficiency,for the control of S.furcifera populations using the combination of a low dose of I.javanica(1×10^(4) conidia mL^(-1))and a low dose of dinotefuran(~4.8-19.2%of normal field use levels),with control effects of>90%and a population level under 50 insects per 100 hills at 3-14 days post-treatment.Our findings indicate that the entomogenous fungus I.javanica offers an attractive biological control addition as part of the integrated pest management(IPM)practices for the control of rice plant pests.展开更多
Beauvericin was analyzed in three forms of the Lepidoptra pathogenic fungus Isaria tenuipes(4 isolates):(a)natural specimen,(b)cultivated synnemata on rice media,and(c)mycelia from fermentation in liquid media.Beauver...Beauvericin was analyzed in three forms of the Lepidoptra pathogenic fungus Isaria tenuipes(4 isolates):(a)natural specimen,(b)cultivated synnemata on rice media,and(c)mycelia from fermentation in liquid media.Beauvericin was detected in very low amounts in all tested natural specimens.Synnemata on rice contained much higher concentrations of beauvericin than the corresponding natural materials,although the concentrations were lower than mycelia from liquid fermentation.The results casted a caution that beauvericin concentration should be carefully checked,as a possible toxic constituent,upon mass production of a selected strain of Isaria tenuipes for health food purposes.展开更多
[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different ca...[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.展开更多
目的对细脚棒束孢Isaria tenuipes次级代谢产物进行研究,为扩大细脚棒束孢的药用资源提供科学依据。方法采用硅胶柱层析,Sephad ex LH-20分离技术进行分离纯化单体化合物,根据理化性质及波谱数据等手段鉴定其结构。结果从细脚棒束孢Isar...目的对细脚棒束孢Isaria tenuipes次级代谢产物进行研究,为扩大细脚棒束孢的药用资源提供科学依据。方法采用硅胶柱层析,Sephad ex LH-20分离技术进行分离纯化单体化合物,根据理化性质及波谱数据等手段鉴定其结构。结果从细脚棒束孢Isaria tenuipes固体发酵产物的乙酸乙酯提取物中分离得到5个化合物,分别是:4-methoxy-6-entanoyl-2H-pyran-2-one(1),6-(1,2-dihydroxypentyl)-4-methoxy-5,6-dihydro-2H-pyran-2-one(2),6-[(3R)-2,3-dihydr oxybutan-2-yl]-4-methoxy-2H-pyran-2-one(3),(5-hydroxy-2-oxo-2H-pyran-4-yl)methylacetate(4)和6-(1-hydroxypentyl)-4-methoxy-5,6-dihydro-2H-pyran-2-one(5)。结论其中化合物1为新的天然产物。其余化合物均是首次从细脚棒束孢中分离得到。展开更多
Isaria cicadae Miquel(IcM)is an edible fungus used in traditional chinese medicine.in this study,we identified the nutritional components of IcM using classical analytical methods and investigated the protective effec...Isaria cicadae Miquel(IcM)is an edible fungus used in traditional chinese medicine.in this study,we identified the nutritional components of IcM using classical analytical methods and investigated the protective effects and mechanisms of intestinal mucosal inflammation mitigation and repair capacity in a dextran sulfate sodium(Dss)-induced ulcerative colitis(Uc)mouse model.IcM,mesalazine alone,and co-administration significantly reduced colonic pathological damage,downregulated inflammatory factors(anti-tumor necrosis factorα(tNF-α),interleukin-1β(iL-1β),high mobility group box 1(HMGB1),nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),secretory immunoglobulin A(sigA)),and improved the severity of Uc in a dose-dependent manner by regulating the interaction between glucocoriticoid receptor(GR)and key proteins in the Hippo pathway,remodeling the intestinal mucosal barrier tissue structure by increasing the expression of mucin 2(MUc2),occludin,zonula occluden-1(Zo-1),and E-cadherin,whereas high-dose IcM treatment increased significantly.Moreover,IcM and mesalazine have a synergistic effect,weaken toxicity to the liver and kidney,and contribute to alleviating the disease process in clinical patients.展开更多
Objective To evaluate the prophylaxis and therapy of Isaria felina(IF) on glycerin-induced acute renal failure(ARF) in rats.Methods Forty male Wistar rats were divided into control,model,Uremic Clearance Granule(UCG,p...Objective To evaluate the prophylaxis and therapy of Isaria felina(IF) on glycerin-induced acute renal failure(ARF) in rats.Methods Forty male Wistar rats were divided into control,model,Uremic Clearance Granule(UCG,positive control),high-and low-dose IF groups.Rats in the high-and low-dose IF groups were ig administered with 200 and 100 mg/kg IF,respectively,while rats were ig administered with 3.6 g/kg UCG successively for 7 d to establish UCG group.The rats in model,control,and drug-treated groups were im injected with 8 mL/kg 50% glycerin after drinking was quitted for 24 h to induce ARF in rats.The drugs were continued to give thereafter.The level of blood urea nitrogen(BUN) and serum creatinine(SCr) were determined 24 and 72 h after the injection of glycerin,also the kidney was dissected for pathology examination.Results Im injection with 8 mL/kg 50% glycerin could successfully induce ARF in rats.The dose of 200 mg/kg IF could reduce the high levels of BUN and SCr,and ameliorate the pathological damage of the kidney.Conclusion IF has good protective and therapeutic effects on ARF and it is a potential and valuable Chinese herb for ARF.展开更多
基金supported by China Spark Program (No.2015GA700011)。
文摘This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabetes(T2DM). Random amplified polymorphic DNA, sequence-characterized amplified region, and high-performance liquid chromatography(HPLC) were used for the quality control of Ic-17-7fb. The pharmacological effects on streptozocin(STZ)-induced high fat diet(HFD)-fed Albino Wistar rats were evaluated. The rats underwent the following treatments: control, metformin, Ic-17-7fb(0.166 and 0.5 g·kg;) or without treatment. The fasting blood glucose(FBG), blood glucose, total cholesterol(TC), triglyceride(TG), high-density lipoprotein(HDL-c), and low-density lipoprotein(LDL-c) were measured. Ic-17-7fb amplified a single specific band by S11-2-F3 and S11-2-R3 primers. An HPLC-based quality and quantity method was established for industrial application. The contents of adenosine and N;-(2-hydroxyethyl) adenosine(HEA) of the cultivated Ic-17-7fb were analyzed. All of the validation lots of cultured Ic-17-7fb passed the quantity control of the training set(0.90 mg·g;of adenosine and 0.89 mg·g;of HEA). After two weeks of administration, the average FBG was 4.89 ± 0.42(control), 26.10 ± 5.77(model), 23.63 ± 6.15(metformin), 17.96 ± 9.36(Ic-17-7fb for 0.166 g·kg;), and 19.69 ±8.71 mmol·L;(Ic-17-7fb for 0.5 g·kg;). The FBG of Ic-17-7fb(0.166 g·kg;) treatment significantly reduced by 31.19%, compared with the model after two weeks of administration(P < 0.01). Metformin, Ic-17-7fb(0.166 g·kg;), and Ic-17-7fb(0.5 g·kg;) reduced TC, TG, HDL-c, and LDL-c compared with the T2DM model treatment at the 6 th week of treatment(P < 0.05). This study established the first quality standard for Ic-17-7fb, which can be effectively applied in the treatment of T2DM. The reliable quality control method and pharmacological effect will broaden its application space.
基金Supported by Innovation Fund for Technology-Based Firms of Yangjiang(2015002)China Spark Program(2015GA780011)+2 种基金Research and Production Cooperation Project,Guangdong(2015B090903042)Welfare Research and Capacity Building Foundation,Guangdong(2017A020208060)Special Funds for Agriculture and Rural Work,Guangdong Province(Agricultural Construction and Reform and Development 2017[No.104],Agriculture Department and Finance Department,Guangdong)
文摘The influence of twelve different pesticides(six insecticides and six fungicides) on lipase production by Isaria fumosorosea was investigated under laboratory conditions. Imidacloprid caused higher reduction in enzyme activities at all three concentrations whereas very low reduction in enzyme activities was caused by azadrichtin and Indoxacarb when used at 10μg/ml. In case of fungicides, propiconazole and pyraclostrobin caused higher reduction in enzyme activities at all three concentrations whereas low reduction in enzyme activities was caused by chlorothalonil and mancozeb when used at 10 μg/ml. Different insecticides significantly affected the toxicity of I. fumosorosea lipase against second instar Dysmicoccus neobrevipesnymphs. I.fumosorosea lipase combined with azadrichtin was proved to be the most aggressive, and those from control were the least virulent with a mean ST50 value of 6.34 ± 0.41 d.
基金funded by grants from the Science and Technology Planning Project of Guangzhou,China(202002020029)the Science and Technology Planning Project of Guangdong Province,China(2019B020217003)+1 种基金the National Key R&D Program of China(2018YFD02003)the National Key Technology Support Program of China(201303019-02)。
文摘The rice planthopper,Sogatella furcifera,is a piercing-sucking insect pest of rice,Oryza sativa.It is responsible for significant crop yield losses,and has developed moderate to high resistance to several commonly used chemical insecticides.We investigated the effects of the insect fungal pathogen Isaria javanica,alone and in combination with the chemical insecticide dinotefuran,on S.furcifera under both laboratory and field conditions.Our results show that I.javanica displays high infection efficiency and mortality for different stages of S.furcifera,reducing adult survival,female oviposition and ovary development.Laboratory bioassays showed that the combined use of I.javanica with a low dose(4-16 mg L^(-1))of dinotefuran resulted in higher mortality in S.furcifera than the use of I.javanica or dinotefuran alone.The combined treatment also had more significant effects on several host enzymes,including superoxide dismutase,catalase,peroxidase,and prophenol oxidase activities.In field trials,I.javanica effectively suppressed populations of rice planthoppers to low levels(22-64%of the level in untreated plots).Additional field experiments showed synergistic effects,i.e.,enhanced efficiency,for the control of S.furcifera populations using the combination of a low dose of I.javanica(1×10^(4) conidia mL^(-1))and a low dose of dinotefuran(~4.8-19.2%of normal field use levels),with control effects of>90%and a population level under 50 insects per 100 hills at 3-14 days post-treatment.Our findings indicate that the entomogenous fungus I.javanica offers an attractive biological control addition as part of the integrated pest management(IPM)practices for the control of rice plant pests.
基金Financial support from the National Center for Genetic Engineering and Biotechnology(BIOTEC)is gratefully acknowledged.
文摘Beauvericin was analyzed in three forms of the Lepidoptra pathogenic fungus Isaria tenuipes(4 isolates):(a)natural specimen,(b)cultivated synnemata on rice media,and(c)mycelia from fermentation in liquid media.Beauvericin was detected in very low amounts in all tested natural specimens.Synnemata on rice contained much higher concentrations of beauvericin than the corresponding natural materials,although the concentrations were lower than mycelia from liquid fermentation.The results casted a caution that beauvericin concentration should be carefully checked,as a possible toxic constituent,upon mass production of a selected strain of Isaria tenuipes for health food purposes.
基金Supported by"Six Talents Peaks"Project in Jiangsu Province in 2015(NY-024)Project of Jiangsu Polytechnic College of Agriculture and Forestry(2014KJ28)
文摘[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.
基金supported by the National Natural science Foundation of china(81673918)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education institutions(2011ZYX6-006)the Project of science and technology of Jiangsu(BY2015008-03).
文摘Isaria cicadae Miquel(IcM)is an edible fungus used in traditional chinese medicine.in this study,we identified the nutritional components of IcM using classical analytical methods and investigated the protective effects and mechanisms of intestinal mucosal inflammation mitigation and repair capacity in a dextran sulfate sodium(Dss)-induced ulcerative colitis(Uc)mouse model.IcM,mesalazine alone,and co-administration significantly reduced colonic pathological damage,downregulated inflammatory factors(anti-tumor necrosis factorα(tNF-α),interleukin-1β(iL-1β),high mobility group box 1(HMGB1),nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),secretory immunoglobulin A(sigA)),and improved the severity of Uc in a dose-dependent manner by regulating the interaction between glucocoriticoid receptor(GR)and key proteins in the Hippo pathway,remodeling the intestinal mucosal barrier tissue structure by increasing the expression of mucin 2(MUc2),occludin,zonula occluden-1(Zo-1),and E-cadherin,whereas high-dose IcM treatment increased significantly.Moreover,IcM and mesalazine have a synergistic effect,weaken toxicity to the liver and kidney,and contribute to alleviating the disease process in clinical patients.
文摘Objective To evaluate the prophylaxis and therapy of Isaria felina(IF) on glycerin-induced acute renal failure(ARF) in rats.Methods Forty male Wistar rats were divided into control,model,Uremic Clearance Granule(UCG,positive control),high-and low-dose IF groups.Rats in the high-and low-dose IF groups were ig administered with 200 and 100 mg/kg IF,respectively,while rats were ig administered with 3.6 g/kg UCG successively for 7 d to establish UCG group.The rats in model,control,and drug-treated groups were im injected with 8 mL/kg 50% glycerin after drinking was quitted for 24 h to induce ARF in rats.The drugs were continued to give thereafter.The level of blood urea nitrogen(BUN) and serum creatinine(SCr) were determined 24 and 72 h after the injection of glycerin,also the kidney was dissected for pathology examination.Results Im injection with 8 mL/kg 50% glycerin could successfully induce ARF in rats.The dose of 200 mg/kg IF could reduce the high levels of BUN and SCr,and ameliorate the pathological damage of the kidney.Conclusion IF has good protective and therapeutic effects on ARF and it is a potential and valuable Chinese herb for ARF.
