Two unusual sesquiterpenoids,irpexlactones A(1)and B(2),were isolated from cultures of the basidiomycete Irpex lacteus.Their structures were established by means of spectroscopic methods,the single crystal X-ray diffr...Two unusual sesquiterpenoids,irpexlactones A(1)and B(2),were isolated from cultures of the basidiomycete Irpex lacteus.Their structures were established by means of spectroscopic methods,the single crystal X-ray diffraction,as well as electronic circular dichroism(ECD)calculations.They possess a novel carbon skeleton with a 5/6/3-fused ring system that may derive from tremulane type sesquiterpenoids with ring-rearrangement.Both compounds show significant inhibitory activities against nitric oxide production with half maximal inhibitory concentration(IC50)values of 2.2 and 1.4μmol/L,respectively.Their anti-inflammatory effects were further evaluated by enzyme-linked immunosorbent assay(ELISA)and Western blot.展开更多
Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese medicine.Seven water-soluble polysaccharide fractions（ILN I,ILN II,ILN III,ILA I,ILA II,ILB I and ILB II） were isol...Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese medicine.Seven water-soluble polysaccharide fractions（ILN I,ILN II,ILN III,ILA I,ILA II,ILB I and ILB II） were isolated and purified from Irpex lacteus by hot-water extraction,deproteinization,decolorization,dicthy laminoethyl（DEAE）-cellulose ion exchange and sephadex G100 chromatographies,respectively.The average molecular weights and monosaccharide composition of these polysaccharide fractions greatly differed from each other.The antitumor and antinephritis activities of the seven polysaccharide fractions were evaluated.It was found that ILN III displayed significant inhibition effects on both humar hepatocellular liver carcinoma（HepG2） and hentietta lacks（HeLa） tumor cells with IC 50 values of 60.95 and 99.95 μg/mL,respectively.ILA I exhibited significant inhibition effects on murine mesangial cells（HBZT-1） with an IC 50 value of 185.06 μg/mL.The inhibition effects of other polysaccharide fractions on these three cells were significantly different.These results suggest that the polysaccharide fractions isolated from Irpex lacteus have potential antitumor and antinephritis activities.展开更多
Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers I...Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off.展开更多
Four eburicane-type triterpenoids with malonyl modifications,namely irpexoates A-D(1-4),were isolated from the fruiting bodies of the medicinal fungus Irpex lacteus.The structures of the new compounds were established...Four eburicane-type triterpenoids with malonyl modifications,namely irpexoates A-D(1-4),were isolated from the fruiting bodies of the medicinal fungus Irpex lacteus.The structures of the new compounds were established by extensive spectroscopic methods,including 1D and 2D NMR,HRESIMS spectroscopic analysis.Irpexoate B(2)displayed weak cytotoxicity against four human cancer cell lines(A-549,SMMC-7721,MCF-7,SW480)with IC50 values varying from 22.9 to 34.0 pM,and irpexoate D(4)showed weak cytotoxicity against the human cancer cell line SW480 with an IC50 value of 35.2μM.展开更多
A new irlactane-type,namely irlactin K(1),and 22 tremulane-type sesquiterpenes including fourteen previously undescribed ones,namely irpexolactins A-N(2-15),and a known irlactane-type sesquiterpenoid,were isolated fro...A new irlactane-type,namely irlactin K(1),and 22 tremulane-type sesquiterpenes including fourteen previously undescribed ones,namely irpexolactins A-N(2-15),and a known irlactane-type sesquiterpenoid,were isolated from the fermentation broth of the medicinal fungus Irpex lacteus HFG1102.The structures of all the isolates were characterized by extensive spectroscopic methods,including 1D and 2D NMR and MS spectroscopic analysis.The absolute configurations of irlactin K and the known compound conocenol B(20)were established by single-crystal X-ray diffraction analysis.The vasorelaxant effects of irlactin K(1),irpexolactins A(2),C(4),K(12),and irlactam(22)were evaluated.展开更多
Eight new furan derivatives,irpexins A‒H(1‒8),two new polyketides,irpexins I and J(9 and 10),together with nine known compounds were isolated from the fermentation of Irpex lacteus.The structures and absolute configur...Eight new furan derivatives,irpexins A‒H(1‒8),two new polyketides,irpexins I and J(9 and 10),together with nine known compounds were isolated from the fermentation of Irpex lacteus.The structures and absolute configurations were elucidated on the basis of extensive spectroscopic methods and Mosher ester reaction.All compounds shows no cytotoxicity to human MCF-7 and Hela cancer cell lines at the concentration of 10μM.展开更多
Six previously undescribed 5,6-seco-tremulane analogues,together with two known ones,were isolated from the culture broth of the medicinal fungus Irpex lacteus HFG1102.The structures of the new compounds were elucidat...Six previously undescribed 5,6-seco-tremulane analogues,together with two known ones,were isolated from the culture broth of the medicinal fungus Irpex lacteus HFG1102.The structures of the new compounds were elucidated via extensive spectroscopic methods,including NMR and HRMS spectroscopic analyses.展开更多
Traditional autoclaving,slow degradation rate and preservation of biomass treated by fungi are the main factors restricting biological treatment.In our previous studies,strains with high efficiency and selective ligni...Traditional autoclaving,slow degradation rate and preservation of biomass treated by fungi are the main factors restricting biological treatment.In our previous studies,strains with high efficiency and selective lignin degradation ability were obtained.To further solve the limiting factors of biological treatment,this paper proposed a composite treatment technology,which could replace autoclaves for fungal treatment and improve the preservation and utilization of fungal-pretreated straw.The autoclaved and expanded buckwheat straw were,respectively,degraded by Irpex lacteus for 14 days(CIL,EIL),followed by ensiling of raw materials(CK)and biodegraded straw of CIL and EIL samples with Lactobacillus plantarum for different days,respectively(CP,CIP,EIP).An expansion led to lactic acid bacteria,mold,and yeast of the samples below the detection line,and aerobic bacteria was significantly reduced,indicating a positive sterilization effect.Expansion before I.lacteus significantly enhanced lignin selective degradation by about 6%,and the absolute content of natural detergent solute was about 5%higher than that of the CIL.Moreover,EIL decreased pH by producing higher organic acids.The combination treatment created favorable conditions for ensiling.During ensiling,EIP silage produced high lactic acid about 26.83 g/kg DM and the highest acetic acid about 22.35 g/kg DM,and the pH value could be stable at 4.50.Expansion before I.lacteus optimized the microbial community for ensiling,resulting in EIP silage co-dominated by Lactobacillus,Pediococcus and Weissella,whereas only Lactobacillus was always dominant in CP and CIP silage.Clavispora gradually replaced Irpex in EIP silage,which potentially promoted lactic acid bacteria growth and acetic acid production.In vitro gas production(IVGP)in EIL was increased by 30%relative to CK and was higher than 24%in CIL.The role of expansion was more significant after ensiling,the IVGP in EIP was increased by 22%relative to CP,while that in CIP silage was only increased by 9%.Silage of fungal-treated samples reduced methane emissions by 28%to 31%.The study demonstrated that expansion provides advantages for fungal colonization and delignification,and further improves the microbial community and fermentation quality for silage,enhancing the nutrition and utilization value.This has practical application value for scaling up biological treatment and preserving the fungal-treated lignocellulose.展开更多
目的对均一耙齿菌I rp ex lacteus糖蛋白I1-2-1进行化学研究。方法利用化学方法和光谱方法分析其结构特征。结果I1-2-1的相对分子质量为40 000,由A ra、X y l、M an、G a l、G lu组成,甲基化分析表明I1-2-1的主链主要以1→2,1→6 linked...目的对均一耙齿菌I rp ex lacteus糖蛋白I1-2-1进行化学研究。方法利用化学方法和光谱方法分析其结构特征。结果I1-2-1的相对分子质量为40 000,由A ra、X y l、M an、G a l、G lu组成,甲基化分析表明I1-2-1的主链主要以1→2,1→6 linked M anp为主。结论I1-2-1为结构复杂的糖蛋白,为首次从耙齿菌中获得。展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.22277147,22177139)the State Key Laboratory of Applied Microbiology South China(No.SKLAM003–2022)。
文摘Two unusual sesquiterpenoids,irpexlactones A(1)and B(2),were isolated from cultures of the basidiomycete Irpex lacteus.Their structures were established by means of spectroscopic methods,the single crystal X-ray diffraction,as well as electronic circular dichroism(ECD)calculations.They possess a novel carbon skeleton with a 5/6/3-fused ring system that may derive from tremulane type sesquiterpenoids with ring-rearrangement.Both compounds show significant inhibitory activities against nitric oxide production with half maximal inhibitory concentration(IC50)values of 2.2 and 1.4μmol/L,respectively.Their anti-inflammatory effects were further evaluated by enzyme-linked immunosorbent assay(ELISA)and Western blot.
基金Supported by the Pharmaceutical Development Fund of China Medical Foundation(No.20061108)
文摘Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese medicine.Seven water-soluble polysaccharide fractions（ILN I,ILN II,ILN III,ILA I,ILA II,ILB I and ILB II） were isolated and purified from Irpex lacteus by hot-water extraction,deproteinization,decolorization,dicthy laminoethyl（DEAE）-cellulose ion exchange and sephadex G100 chromatographies,respectively.The average molecular weights and monosaccharide composition of these polysaccharide fractions greatly differed from each other.The antitumor and antinephritis activities of the seven polysaccharide fractions were evaluated.It was found that ILN III displayed significant inhibition effects on both humar hepatocellular liver carcinoma（HepG2） and hentietta lacks（HeLa） tumor cells with IC 50 values of 60.95 and 99.95 μg/mL,respectively.ILA I exhibited significant inhibition effects on murine mesangial cells（HBZT-1） with an IC 50 value of 185.06 μg/mL.The inhibition effects of other polysaccharide fractions on these three cells were significantly different.These results suggest that the polysaccharide fractions isolated from Irpex lacteus have potential antitumor and antinephritis activities.
文摘Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off.
基金supported by National Natural Science Foundation of China(81561148013)the Key Projects of Technological Innovation of Hubei Province(2016ACA138)the Fundamental Research Funds for the Central University,South-Central University for Nationalities(CZT18014,CZT18013).
文摘Four eburicane-type triterpenoids with malonyl modifications,namely irpexoates A-D(1-4),were isolated from the fruiting bodies of the medicinal fungus Irpex lacteus.The structures of the new compounds were established by extensive spectroscopic methods,including 1D and 2D NMR,HRESIMS spectroscopic analysis.Irpexoate B(2)displayed weak cytotoxicity against four human cancer cell lines(A-549,SMMC-7721,MCF-7,SW480)with IC50 values varying from 22.9 to 34.0 pM,and irpexoate D(4)showed weak cytotoxicity against the human cancer cell line SW480 with an IC50 value of 35.2μM.
基金supported by National Natural Science Foundation of China(Nos.81903512,21961142008).
文摘A new irlactane-type,namely irlactin K(1),and 22 tremulane-type sesquiterpenes including fourteen previously undescribed ones,namely irpexolactins A-N(2-15),and a known irlactane-type sesquiterpenoid,were isolated from the fermentation broth of the medicinal fungus Irpex lacteus HFG1102.The structures of all the isolates were characterized by extensive spectroscopic methods,including 1D and 2D NMR and MS spectroscopic analysis.The absolute configurations of irlactin K and the known compound conocenol B(20)were established by single-crystal X-ray diffraction analysis.The vasorelaxant effects of irlactin K(1),irpexolactins A(2),C(4),K(12),and irlactam(22)were evaluated.
基金This work was financially supported by the joint research project from the National Natural Science Foundation of China(Grant No.21961142008)Thailand Research Fund(Grant No.DBG6280008).
文摘Eight new furan derivatives,irpexins A‒H(1‒8),two new polyketides,irpexins I and J(9 and 10),together with nine known compounds were isolated from the fermentation of Irpex lacteus.The structures and absolute configurations were elucidated on the basis of extensive spectroscopic methods and Mosher ester reaction.All compounds shows no cytotoxicity to human MCF-7 and Hela cancer cell lines at the concentration of 10μM.
基金supported by National Natural Science Foundation of China(81561148013)the Key Projects of Technological Innovation of Hubei Province(No.2016ACA138)the Fundamental Research Funds for the Central University,South-Central University for Nationalities(CZT18014,CZT18013).
文摘Six previously undescribed 5,6-seco-tremulane analogues,together with two known ones,were isolated from the culture broth of the medicinal fungus Irpex lacteus HFG1102.The structures of the new compounds were elucidated via extensive spectroscopic methods,including NMR and HRMS spectroscopic analyses.
文摘Traditional autoclaving,slow degradation rate and preservation of biomass treated by fungi are the main factors restricting biological treatment.In our previous studies,strains with high efficiency and selective lignin degradation ability were obtained.To further solve the limiting factors of biological treatment,this paper proposed a composite treatment technology,which could replace autoclaves for fungal treatment and improve the preservation and utilization of fungal-pretreated straw.The autoclaved and expanded buckwheat straw were,respectively,degraded by Irpex lacteus for 14 days(CIL,EIL),followed by ensiling of raw materials(CK)and biodegraded straw of CIL and EIL samples with Lactobacillus plantarum for different days,respectively(CP,CIP,EIP).An expansion led to lactic acid bacteria,mold,and yeast of the samples below the detection line,and aerobic bacteria was significantly reduced,indicating a positive sterilization effect.Expansion before I.lacteus significantly enhanced lignin selective degradation by about 6%,and the absolute content of natural detergent solute was about 5%higher than that of the CIL.Moreover,EIL decreased pH by producing higher organic acids.The combination treatment created favorable conditions for ensiling.During ensiling,EIP silage produced high lactic acid about 26.83 g/kg DM and the highest acetic acid about 22.35 g/kg DM,and the pH value could be stable at 4.50.Expansion before I.lacteus optimized the microbial community for ensiling,resulting in EIP silage co-dominated by Lactobacillus,Pediococcus and Weissella,whereas only Lactobacillus was always dominant in CP and CIP silage.Clavispora gradually replaced Irpex in EIP silage,which potentially promoted lactic acid bacteria growth and acetic acid production.In vitro gas production(IVGP)in EIL was increased by 30%relative to CK and was higher than 24%in CIL.The role of expansion was more significant after ensiling,the IVGP in EIP was increased by 22%relative to CP,while that in CIP silage was only increased by 9%.Silage of fungal-treated samples reduced methane emissions by 28%to 31%.The study demonstrated that expansion provides advantages for fungal colonization and delignification,and further improves the microbial community and fermentation quality for silage,enhancing the nutrition and utilization value.This has practical application value for scaling up biological treatment and preserving the fungal-treated lignocellulose.
文摘目的对均一耙齿菌I rp ex lacteus糖蛋白I1-2-1进行化学研究。方法利用化学方法和光谱方法分析其结构特征。结果I1-2-1的相对分子质量为40 000,由A ra、X y l、M an、G a l、G lu组成,甲基化分析表明I1-2-1的主链主要以1→2,1→6 linked M anp为主。结论I1-2-1为结构复杂的糖蛋白,为首次从耙齿菌中获得。
