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Enhancing the biodegradation of low-density polyethylene(LDPE)using novel bacterial consortia:Bacillus sp.AS3 and Sphingobacterium sp.AS8
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作者 Pornya Khampratueng Anil Kumar Anal 《Journal of Environmental Sciences》 2026年第1期263-270,共8页
Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,red... Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency. 展开更多
关键词 BiODEGRADATiON Low-density polyethylene Bacillus sp. sphingobacterium sp.
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Revealing inheritance of a Xinjiang isolate BGTB-1 of Puccinia striiformis f.sp.tritici and the shift of pathogenicity from avirulence to virulence at heterozygous AvrYr5 locus
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作者 Lin Wang Fei Liu +3 位作者 Yumeng Bian Mudi Sun Zhensheng Kang Jie Zhao 《Journal of Integrative Agriculture》 2026年第2期744-755,共12页
In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.... In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang. 展开更多
关键词 wheat yellow rust Puccinia striiformis f.sp.tritici sexual reproduction virulence variation new race SNP Yr5 XinJiANG
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羊踯躅内生真菌Penicillium sp.RM的次生代谢产物及其肿瘤细胞增殖抑制活性研究
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作者 王雪 周磊 +3 位作者 胡倚航 刘洋 张雪 刘云宝 《中国中药杂志》 北大核心 2026年第2期444-449,共6页
对羊踯躅内生真菌Penicillium sp.RM的次级代谢产物进行研究,采用硅胶柱色谱、凝胶柱色谱、半制备液相色谱等进行分离纯化,共从Penicillium sp.RM发酵物的乙酸乙酯部位中分离鉴定出10个化合物,利用NMR、MS、UV、IR、CD等现代波谱技术确... 对羊踯躅内生真菌Penicillium sp.RM的次级代谢产物进行研究,采用硅胶柱色谱、凝胶柱色谱、半制备液相色谱等进行分离纯化,共从Penicillium sp.RM发酵物的乙酸乙酯部位中分离鉴定出10个化合物,利用NMR、MS、UV、IR、CD等现代波谱技术确定了化合物的结构,分别为penicidienone A(1)、diversonol(2)、alternariol(3)、alternariol 9-methyl ether(4)、methyla-ted dechloromonilicin(5)、norliquexantona(6)、alternethanoxin A(7)、α-diversonolic ester(8)、(±)-asperlone A(9)、verruculotoxin(10),其中化合物1是1种新的环戊烯酮衍生物。肿瘤细胞增殖抑制活性筛选结果表明化合物9对HepG2、HGC27、U251细胞具有抑制作用,IC50分别为9.7、9.3、8.4μmol·L-1。 展开更多
关键词 羊踯躅 内生真菌 Penicillium sp.RM 次生代谢产物 环戊烯酮
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PBL联合SP模式和Seminar教学法在中医学实践教学中的应用价值
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作者 宇汝翠 吴瑞影 李金虎 《吉林医学》 2025年第6期1526-1528,共3页
目的:分析以问题为基础的教学法(PBL)联合标准化病人(SP)模式和Seminar教学法在中医学实践教学中的应用价值。方法:选取进行中医学实践的50名学生进行研究。以随机数字表法分为两组各25名。对照组采用PBL联合SP模式。观察组采用PBL+SP模... 目的:分析以问题为基础的教学法(PBL)联合标准化病人(SP)模式和Seminar教学法在中医学实践教学中的应用价值。方法:选取进行中医学实践的50名学生进行研究。以随机数字表法分为两组各25名。对照组采用PBL联合SP模式。观察组采用PBL+SP模式+Seminar教学法。比较两组学习效果(知识水平、相关技能水平、学习态度)及学生满意度。结果:观察组知识水平、相关技能水平、学习态度及总评分显著高于对照组(P<0.01)。观察组满意度高于对照组,差异有统计学意义(P<0.05)。结论:PBL联合SP模式和Seminar教学法可提升中医学实践教学的教学质量,学生学习效果好,满意度高。 展开更多
关键词 PBL sp SEMinAR 教学 中医学 实践
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Densification and microstructure evolution during SPS consolidation process in W-Ni-Fe system 被引量:2
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作者 胡可 李小强 +1 位作者 杨超 李元元 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第3期493-501,共9页
Spark plasma sintering method (SPS) was used to consolidate mixed W-5.6Ni-1.4Fe (mass fraction, %) powders from commercial fine elemental powders, and both the densification behavior and microstructure evolution i... Spark plasma sintering method (SPS) was used to consolidate mixed W-5.6Ni-1.4Fe (mass fraction, %) powders from commercial fine elemental powders, and both the densification behavior and microstructure evolution in sintering were investigated at different heating rates. The results show that the SPS densification process can be divided into three stages. At the initial unshrinking stage, fast heating generates instantaneous discharge and locally inhomogeneous temperature distribution in solid-state powder particles, enhancing later densification; during the intermediate solid state sintering stage, diffusion is more sufficient in the slow-heated SPS process; at the final transient liquid-phase sintering stage, tungsten grains become sphered and coarsen rapidly, but fast heating helps maintain rather small grain sizes. 展开更多
关键词 densification behavior microstructure evolution spS W-Ni-Fe alloy
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SPS参数对ZrC-SiC陶瓷微观结构及力学性能的影响
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作者 任静 解静 +4 位作者 王龙 张前 孙国栋 李辉 贾研 《兵器材料科学与工程》 北大核心 2026年第1期34-39,共6页
采用放电等离子烧结(SPS)工艺在不同烧结温度、保温时间、烧结压力下制备了一系列ZrC-SiC复相陶瓷,研究工艺参数对材料微观结构和力学性能的影响。结果表明:随着烧结温度的升高,陶瓷致密度与硬度均有所提升,高于1 500℃后提升缓慢,需增... 采用放电等离子烧结(SPS)工艺在不同烧结温度、保温时间、烧结压力下制备了一系列ZrC-SiC复相陶瓷,研究工艺参数对材料微观结构和力学性能的影响。结果表明:随着烧结温度的升高,陶瓷致密度与硬度均有所提升,高于1 500℃后提升缓慢,需增大压力或延长保温时间来进一步提高致密度,但过高的压力和保温时间会导致材料致密度和力学性能下降。在1 500℃/50 MPa/5 min下制备的ZrC-SiC复相陶瓷展现出优异的力学性能,其相对密度达到99.95%,维氏硬度和弯曲强度分别为21.01 GPa和357.37 MPa。 展开更多
关键词 ZrC-SiC超高温陶瓷 spS 烧结参数 力学性能
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缬草药材内生真菌Aspergillus sp.FH-1中1个新的氯代丁烯内酯类化合物
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作者 樊浩 王鑫 +7 位作者 禹梦欣 吴怡轩 赵丽媛 张可桢 李玉泽 王薇 宋小妹 张东东 《中国中药杂志》 北大核心 2026年第2期450-455,共6页
研究缬草药材内生真菌Aspergillus sp.FH-1大米高盐培养基中抗真菌次生代谢产物及其抗菌活性。采用硅胶和ODS柱色谱以及半制备液相色谱等方法进行分离纯化,并结合其理化性质及NMR、IR、MS等波谱学技术进行结构解析。采用菌丝生长速率法... 研究缬草药材内生真菌Aspergillus sp.FH-1大米高盐培养基中抗真菌次生代谢产物及其抗菌活性。采用硅胶和ODS柱色谱以及半制备液相色谱等方法进行分离纯化,并结合其理化性质及NMR、IR、MS等波谱学技术进行结构解析。采用菌丝生长速率法测定所有化合物对番木瓜炭疽病菌Colletotrichum gloeosporioides的抗真菌活性。从Aspergillus sp.FH-1菌株的乙酸乙酯提取部位分离得到8个化合物,分别鉴定为asperchoried A(1)、butyrolactoneⅡ(2)、isobutyrolactoneⅡ(3)、asperno-lide A(4)、butyrolactoneⅤ(5)、clavatone(6)、1-羟基-6,8-二甲氧基-3-甲基蒽-9,10-二酮(7)、(S)-7-甲氧基-2,5-二甲基-2,3-二氢苯并呋喃-6-醇(8),其中化合物1为氯代丁烯内酯类新化合物。化合物1、2、6、7抑制番木瓜炭疽病菌活性的EC50分别为15.08、77.19、89.94、62.02μg·mL-1,表现出抑制番木瓜炭疽病菌活性。 展开更多
关键词 缬草 Aspergillus sp.FH-1 分离纯化 结构鉴定 asperchoried A 抗真菌
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Effect of Botryosphaeria berengeriana f.sp.piricola and Biocontrol Bacteria on the System of Antioxidant Enzymes in Pears 被引量:1
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作者 张丽丽 常有宏 陈志谊 《Agricultural Science & Technology》 CAS 2015年第9期1833-1836,1882,共5页
[Objective] The aim was to understand defense mechanism of pear after inoculated Botryosphaeria berengeriana f. sp. piricolan and mechanism of antioxidant enzymes of biocontrol bacteria. [Method] Pears were treated by... [Objective] The aim was to understand defense mechanism of pear after inoculated Botryosphaeria berengeriana f. sp. piricolan and mechanism of antioxidant enzymes of biocontrol bacteria. [Method] Pears were treated by Botryosphaeria berengeriana f. sp. piricolan and bio control bacteria, and the change of antioxidant enzymes were determined. [Result] The biocontrol bacteria had little effect on MDA;the content of MDA treated by B. berengeriana reached high peak in 48 h, was 10.22nmol/g which was 1.86 times of CK; the content of MDA treated by B. berengeriana and biocontrol bacteria reached high peak in 24 h, was 8.92 nmol/g which was1.62 times of CK. The content of SOD treated by biocontrol bacteria reached high peak in 48 h, was 126.69 U/[g(FW)·min] which was 1.54 times of CK; the contents of SOD treated by B. berengeriana as well as B. berengeriana and biocontrol bacteria reached high peak in 24 h, were 122.10 and 135.32 U/[g(FW)·min] which were 1.48 and 1.65 times of CK respectively; the contents of POD on biocontrol bacteria treatment, B. ana treatment as well as B. berengeriana and biocontrol bacteria treatment reached high peak in 24 h, were 385.34, 342.50 and 290.00 U/[g(FW)·min] which were 1.83, 1.62 and 1.38 times of CK respectively. The contents of CAT on biocontrol bacteria treatment, B. rengeriana treatment as well as B. berengeriana and biocontrol bacteria treatment reached high peak in 6 h, were 133.33,114.17 and 113.35 U/[g(FW)·min] which were 1.33, 1.14 and 1.13 times of CK respectively. The biocontrol bacteria had little difference in CK; the content of PPO of B. berengeriana treatment reached high peak in 12 h, was 81.86 U/[g(FW)·min]which was 1.76 times of CK; B. berengeriana and biocontrol bacteria treatment reached high peak in 24 h, was 70.00 U/[g(FW)·min] which was 1.50 times of CK.[Conclusion] B. berengeriana and biocontrol bacteria had more effect on MDA; both B. berengeriana and biocontrol bacteria could increase the excitation of SOD enzyme activity; both B. berengeriana and biocontrol bacteria could increase the excitation of POD enzyme activity; both B. berengeriana and biocontrol bacteria could increase the excitation of CAT enzyme activity; using biocontrol bacteria alone had not obvious effect on PPO, B. berengeriana could increase the excitation of PPO enzyme activity. 展开更多
关键词 Botryosphaeria berengeriana f.sp.piricolan Biocontrol bacteria Antioxidant enzymes
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元素标记单颗粒-电感耦合等离子体质谱(SP-ICP-MS)技术的生物分析应用
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作者 郭婷 陈贝贝 +1 位作者 何蔓 胡斌 《中国无机分析化学》 北大核心 2026年第3期376-387,共12页
元素标记电感耦合等离子体质谱(ICP-MS)技术已发展成为一种强大的生物定量分析工具。单颗粒(SP)-ICP-MS基于时间分辨检测模式,能够实现对悬浮液中单个金属纳米粒子(NPs)的精准测定。借助SP-ICP-MS在单颗粒检测方面的独特优势,以金属NPs... 元素标记电感耦合等离子体质谱(ICP-MS)技术已发展成为一种强大的生物定量分析工具。单颗粒(SP)-ICP-MS基于时间分辨检测模式,能够实现对悬浮液中单个金属纳米粒子(NPs)的精准测定。借助SP-ICP-MS在单颗粒检测方面的独特优势,以金属NPs作为元素标签,将元素标记策略与SP-ICP-MS分析技术相结合,成功开发出一类新型的元素标记ICP-MS方法。该方法在核酸、蛋白质等多种生物分子的高灵敏分析中展现出显著潜力。本文系统综述了元素标记SP-ICP-MS技术在生物分析领域的研究进展,重点阐述了异相分析、均相分析及数字检测等不同模式的应用特点。通过对各种检测模式的方法优势、实际应用及技术挑战的深入讨论,旨在为元素标记SP-ICP-MS技术的进一步发展提供参考,并对其在生物分析领域的应用前景进行了展望。 展开更多
关键词 单颗粒(sp-iCP-MS) 元素标记 异相分析 均相分析 数字检测
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Praxillella sp.线粒体全基因组序列与竹节虫科(Maldanidae)的系统发育关系
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作者 龚霄鹏 段晨 +1 位作者 叶莹莹 陈永久 《海洋学报》 北大核心 2026年第1期97-108,共12页
竹节虫科(Maldanidae)物种广泛分布于各种海洋环境,从浅海到深海皆有分布。但由于采集过程中非常容易断裂,很难获得较为完整的样本,因此形态学研究难以充分进行。关于竹节虫的分类地位,不同的研究者存在着不同的观点和争议。为进一步确... 竹节虫科(Maldanidae)物种广泛分布于各种海洋环境,从浅海到深海皆有分布。但由于采集过程中非常容易断裂,很难获得较为完整的样本,因此形态学研究难以充分进行。关于竹节虫的分类地位,不同的研究者存在着不同的观点和争议。为进一步确定竹节虫科的系统发育关系,本研究采用二代基因组测序数据对Praxillella sp.的线粒体基因组进行了组装与分析。结果显示Praxillella sp.基因组全长15539 bp,碱基组成为A(33.7%)、T(32.6%)、G(13.6%)和C(20%),具有明显的AT偏向性。与大多数后生动物类似,Praxillella sp.线粒体基因含有13个蛋白编码基因(PCGs),22个tRNA,2个rRNA和一段控制区(CR)。密码子偏好性可能主要受到自然选择的影响。基于13个PCGs构建的最大似然树和贝叶斯树的扑拓结构基本一致,除了真节虫亚科(Euclymeninae)内的P.praetermissa的位置存在不确定性外,但总体上支持了竹节虫亚科(Maldaninae)的单系性;与之相比,真节虫亚科(Euclymeninae)并未形成单系群,而是一个与征节虫亚科(Nicomachinae)并系的类群,在竹节虫科的14个物种中,共鉴定出4种基因排序方式,其中3种仅为tRNA顺序的变化,通过复制-随机丢失即可完成。本研究结果为厘清Praxillella sp.在竹节虫科中的分类与系统发育地位提供了新的观点。 展开更多
关键词 Praxillella sp. 竹节虫科 线粒体基因组 基因重排 系统发育关系
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C-Root SP、iRoot SP、AH Plus根管封闭剂的临床应用效果比较
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作者 陈铭燕 马春益 孙敬伟 《安徽医专学报》 2026年第1期24-27,共4页
目的:探讨不同根管封闭剂的应用效果。方法:选取医院收治的120例(120牙)牙髓根尖周病患者,依据随机数字表法分为A组(40例,40牙,C-Root SP根管封闭剂结合单尖法)、B组(40例,40牙,iRoot SP根管封闭剂结合单尖法)、C组(40例,40牙,AH Plus... 目的:探讨不同根管封闭剂的应用效果。方法:选取医院收治的120例(120牙)牙髓根尖周病患者,依据随机数字表法分为A组(40例,40牙,C-Root SP根管封闭剂结合单尖法)、B组(40例,40牙,iRoot SP根管封闭剂结合单尖法)、C组(40例,40牙,AH Plus热牙胶垂直加压法)。三组均观察至术后1周,比较三组疗效指标。结果:术中、术后1 d,C组患者中重度疼痛占比高于A组、B组(P<0.05);术中,C组患者无痛占比低于A组、B组,术后1 d,C组患者无痛占比低于A组(P<0.05);A组、B组患者恰填率高于C组,超填率均低于C组(P<0.05);A组、B组患者单根管充填时间短于C组(P<0.05)。结论:相较于AH Plus热牙胶充填法,C-Root SP、iRoot SP单尖充填法可缓解患者疼痛程度,提高恰填率,降低超填率,节省单根管充填时间。 展开更多
关键词 牙髓根尖周病 C-Root sp iRoot sp AH Plus
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西沙软珊瑚Sinularia sp.倍半萜类次级代谢产物研究
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作者 陈海燕 任俊德 +1 位作者 唐旭利 李国强 《中国海洋药物》 2025年第3期67-74,共8页
目的对采自西沙群岛的软珊瑚样品Sinularia sp.进行倍半萜类次级代谢产物的研究。方法利用硅胶柱层析、薄层色谱、中压制备型液相色谱和高效液相色谱等分离方法,对软珊瑚中的化学成分进行提取、分离并纯化;通过计算ECD、X射线单晶衍射... 目的对采自西沙群岛的软珊瑚样品Sinularia sp.进行倍半萜类次级代谢产物的研究。方法利用硅胶柱层析、薄层色谱、中压制备型液相色谱和高效液相色谱等分离方法,对软珊瑚中的化学成分进行提取、分离并纯化;通过计算ECD、X射线单晶衍射方法确定新化合物1~2的绝对构型,综合运用核磁共振波谱、质谱等现代波谱学方法,通过与文献报道的数据进行对比,鉴定已知化合物3~8的结构。并对化合物进行生物活性检测。结果从西沙群岛软珊瑚Sinularia sp.中分离鉴定8个倍半萜类化合物,包括2个新的倍半萜sinularnoid A(1)、sinularnoid B(2),6个已知的倍半萜类化合物,分别为sinuhirtin F(3)、nanonorcaryophyllene B(4)、sinuhirtin G(5)、nanonorcaryophyllene A(6)、methyl-(1S^(*),4S^(*),5S^(*),9R^(*),11S^(*))-4,5-epoxycaryophyllen-13-oate(7)、(1R,4R,5R,9S,11R)-4,5-epoxycaryophyll-8(14)-en-12-oic acid(8)。结论从西沙群岛软珊瑚Sinularia sp.中分离得到8个倍半萜类化合物,其化学结构类型分别属于caryophyllene型和norcaryophyllene型,其中化合物1~2为新化合物。活性测试结果表明,化合物7在斑马鱼实验中具有微弱的抗炎活性。 展开更多
关键词 Sinularia sp. 倍半萜 次级代谢产物
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Design of PLC control system of RTG spreader in Qingdao Port Container Terminal
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作者 王毅 朱德平 于晓良 《Journal of Measurement Science and Instrumentation》 CAS 2014年第1期61-65,共5页
The control method of rubber tyre gantry (RTG) spreader in Qingdao Port Container Terminal is logic board control,which has many shortcomings such as expensive spare parts and high faults.This paper designs a new co... The control method of rubber tyre gantry (RTG) spreader in Qingdao Port Container Terminal is logic board control,which has many shortcomings such as expensive spare parts and high faults.This paper designs a new control system using programmable logic controller (PLC) centralized control to replace the original logic board control.The new system mainly contains complete ELME spreader control scheme design,hardware selection and PLC control program development.Its field application shows that the system has characteristics of high efficiency,low running cost,easy maintenance. 展开更多
关键词 rubber tyre gantry (RTG) spREADER circuit board programmable logic controller (PLC)
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盐溶液中微塑料的SP-ICP-MS分析方法及应用
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作者 王金莹 栾浩轩 +4 位作者 #王春慧 朱敏 徐妍 毛雪飞 李雪 《中国无机分析化学》 北大核心 2026年第3期442-452,共11页
微塑料作为新污染物在盐类产品中的污染已引起广泛关注,但高盐基质导致的信号抑制与传输效率低下等问题长期制约微塑料的精准检测。本研究建立了一种基于单颗粒电感耦合等离子体质谱(SP-ICP-MS)的高盐基质微塑料直接分析方法,以13C为检... 微塑料作为新污染物在盐类产品中的污染已引起广泛关注,但高盐基质导致的信号抑制与传输效率低下等问题长期制约微塑料的精准检测。本研究建立了一种基于单颗粒电感耦合等离子体质谱(SP-ICP-MS)的高盐基质微塑料直接分析方法,以13C为检测元素,采用高效进样系统、线性直通雾化室、低雾化气流速(0.250 L/min)及超短驻留时间(50μs),结合基质匹配校准,将微塑料传输效率提升至61%±15%,显著降低了盐对检测的干扰。方法学验证表明,针对0.90%(W∶V)盐水基质中1~10μm聚苯乙烯微塑料,粒径检出限0.44μm,粒径偏差≤12%,加标回收率92.0%~111%,方法精密度与准确度良好。应用于市售盐类产品检测,生理盐水微塑料粒径为1.31~1.37μm,浓度为79~334 particles/mL;食用盐水溶液样品中粒径1.22~1.30μm,浓度为79~629 particles/mL,其中JS地区食盐中微塑料含量最高。本研究为盐类产品中微塑料的高通量精准检测提供了可靠技术手段,也为企业质量控制和监管部门风险评估提供重要数据支撑。 展开更多
关键词 微塑料 sp-iCP-MS 盐水基质 基体匹配 传输效率 定量分析 健康风险
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红豆杉内生真菌Penicillium sp.HDS-P-2次级代谢产物的分离与鉴定
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作者 袁春蕾 李秋迎 +3 位作者 杨金源 马嘉阳 白皎 华会明 《沈阳药科大学学报》 2026年第1期53-60,69,共9页
目的研究红豆杉[Taxus chinensis(Pilg.)Rehder]内生真菌Penicillium sp.HDS-P-2中的次级代谢产物,评价化合物的肿瘤细胞毒作用。方法采用硅胶、ODS、Sephadex LH-20凝胶、MCI柱色谱及高效液相色谱等手段进行分离纯化,根据理化性质和波... 目的研究红豆杉[Taxus chinensis(Pilg.)Rehder]内生真菌Penicillium sp.HDS-P-2中的次级代谢产物,评价化合物的肿瘤细胞毒作用。方法采用硅胶、ODS、Sephadex LH-20凝胶、MCI柱色谱及高效液相色谱等手段进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。以顺铂为阳性对照药,通过CCK-8法测定化合物对HL-60细胞的生长抑制作用。结果分离得到16个化合物,分别鉴定为penimeroterpenoid A(1)、peniandrastin G(2)、andrastin A(3)、shearinine S(4)、shearinine E(5)、shearinine A(6)、22,23-dehydroshearinine A(7)、shearinine D(8)、hypoxyterpoid A(9)、paecilomycine B(10)、penioxalicin(11)、7-羟基-4-甲氧基-5-甲基香豆素(12)、硫赭曲菌素(13)、O-methylcurvulinic acid(14)、aspernigrin A(15)、pyrophen(16)。化合物5~8对HL-60细胞的IC50为10~20μmol·L^(-1)。结论化合物12为首次从青霉属真菌中分离得到,化合物2、4、7、10、11为首次从红豆杉内生菌中分离得到。并且化合物1、5~8对HL-60细胞表现出不同程度的抑制作用。 展开更多
关键词 红豆杉内生真菌 青霉属 次级代谢产物 结构鉴定 细胞毒活性
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Reprogramming rate-dependent stress-strain curves of mechanical metamaterials by“stair-building”strategy
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作者 Xin Lin Fei Pan +5 位作者 Jintao Du Ke Ma Juan Guan Xiangchao Feng Pengfei Wang Yuli Chen 《Acta Mechanica Sinica》 2026年第1期251-259,共9页
Metamaterials programmed with target rate-dependent mechanical properties are efficient platforms for realizing advanced functionalities.Yet,the loading rate-dependent mechanical property programming has received limi... Metamaterials programmed with target rate-dependent mechanical properties are efficient platforms for realizing advanced functionalities.Yet,the loading rate-dependent mechanical property programming has received limited attention.Here,the“stair-building”strategy is employed in the rate domain by combining the bistability with viscoelasticity.An arbitrary target curve in the programmable space can be approximated by a“stair”built by two kinds of“bricks”.The“bricks”can be realized by a dual-bistable unit,constructed by two bistable structures in series.The dual-bistable unit can switch between two efficient stable phases without inducing changes in the global morphology.Such a unit exhibits N-shaped stress-strain curves at both efficient stable phases with different peak values,resulting in different heights of“bricks”.Moreover,the N-shaped curves have rate-dependent peak values,indicating that the heights of“bricks”change with loading rate.The“stair-building”strategy is realized by array-structured mechanical metamaterials based on dual-bistable units.Different stress-strain curves under various loading rates can be reprogrammed in the same piece of metamaterial by intentionally selecting the efficient stable phases of units.Besides,the rate effect of the metamaterial can also be tuned by reprogramming stress-strain curves under both low and high loading rates,respectively.This reprogrammable metamaterial is promising in smart vibration isolators and adaptive energy absorbers. 展开更多
关键词 Mechanical metamaterial programmability Rate-dependent effect
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P4LoF: Scheduling Loop-Free Multi-Flow Updates in Programmable Networks
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作者 Jiqiang Xia Qi Zhan +2 位作者 Le Tian Yuxiang Hu Jianhua Peng 《Computers, Materials & Continua》 2026年第1期1236-1254,共19页
The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.H... The rapid growth of distributed data-centric applications and AI workloads increases demand for low-latency,high-throughput communication,necessitating frequent and flexible updates to network routing configurations.However,maintaining consistent forwarding states during these updates is challenging,particularly when rerouting multiple flows simultaneously.Existing approaches pay little attention to multi-flow update,where improper update sequences across data plane nodes may construct deadlock dependencies.Moreover,these methods typically involve excessive control-data plane interactions,incurring significant resource overhead and performance degradation.This paper presents P4LoF,an efficient loop-free update approach that enables the controller to reroute multiple flows through minimal interactions.P4LoF first utilizes a greedy-based algorithm to generate the shortest update dependency chain for the single-flow update.These chains are then dynamically merged into a dependency graph and resolved as a Shortest Common Super-sequence(SCS)problem to produce the update sequence of multi-flow update.To address deadlock dependencies in multi-flow updates,P4LoF builds a deadlock-fix forwarding model that leverages the flexible packet processing capabilities of the programmable data plane.Experimental results show that P4LoF reduces control-data plane interactions by at least 32.6%with modest overhead,while effectively guaranteeing loop-free consistency. 展开更多
关键词 Network management update consistency programmable data plane P4
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Hangzhou strategic action plan charts a decade for biosphere reserves
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作者 Ke-Ping Ma 《Plant Diversity》 2026年第1期216-218,共3页
In a pivotal moment for global conservation policy,UNESCO’s Man and the Biosphere(MAB)Programme has endorsed the Hangzhou Strategic Action Plan(HSAP)2026–2035(UNESCO,2025),a decade-long roadmap that positions the Wo... In a pivotal moment for global conservation policy,UNESCO’s Man and the Biosphere(MAB)Programme has endorsed the Hangzhou Strategic Action Plan(HSAP)2026–2035(UNESCO,2025),a decade-long roadmap that positions the World Network of Biosphere Reserves(WNBR)as central actors in delivering biodiversity,climate and sustainable-development goals(SDGs).The plan—developed and opened for global consultation in the runup to the 5th World Congress of Biosphere Reserves—articulates strategic directions,measurable action targets and practical instruments to strengthen biosphere reserves as living laboratories for people and nature(Ma,2025). 展开更多
关键词 Man and Biosphere Programme World Network of Biosphere Reserve Sustainable development Kunming-Montreal Global Biodiversity Framework
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A Boon for Trade in Services
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作者 ZHANG SHASHA 《ChinAfrica》 2026年第3期22-24,共3页
Saud Khan,a Pakistani physician,collected his work and residence permits at the Yangpu Government Service Centre in Danzhou City,Hainan Province,on 18 December 2025.Khan had arrived in Hainan three months earlier to p... Saud Khan,a Pakistani physician,collected his work and residence permits at the Yangpu Government Service Centre in Danzhou City,Hainan Province,on 18 December 2025.Khan had arrived in Hainan three months earlier to participate in a medical training programme at Hainan Western Central Hospital.By the time the programme concluded,he had made up his mind to stay and broaden his professional experience and long-term career prospects in Hainan. 展开更多
关键词 TRADE residence permit Hainan province work residence permits work permit Yangpu government service centre medical training programme SERViCES
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Ultrahigh strength of cage-like polymeric nitrogen surpassing diamond under high pressure
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作者 Hui Liang Di Wang +4 位作者 Rui Xu Hao Chen Dan Zhou Yunwei Zhang Quan Li 《Matter and Radiation at Extremes》 2026年第1期103-110,共8页
We report first-principles predictions of a cage-like polymeric nitrogen phase(cage-N)composed of interlocked N10 clusters stabilized by mixed sp^(2)/sp^(3) hybridization.Under high pressure,cage-N exhibits exceptiona... We report first-principles predictions of a cage-like polymeric nitrogen phase(cage-N)composed of interlocked N10 clusters stabilized by mixed sp^(2)/sp^(3) hybridization.Under high pressure,cage-N exhibits exceptional mechanical performance,including an ideal compressive strength of 343 GPa at a pressure of 300 GPa,~33% higher than that of diamond.This ultrahigh strength arises from the synergistic interplay between its three-dimensional covalent framework and hybridized bonding topology,which enables isotropic stress accommodation and dynamic electronic rearrangement.These results establish cage-N as a promising non-carbon ultrahard material and provide a bonding-driven route toward designing superhard frameworks under extreme conditions. 展开更多
关键词 compressive strength mixed sp sp hybridization cage polymeric nitrogen hybridized bonding topologywhich mechanical performance interlocked n clusters ultrahigh strength first principles predictions
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