[Objective] The paper aimed to explore a simple in situ PCR technology for buckwheat.[Method] By using 16S and 4.5S nested primers and psbA primer,the in situ PCR,nested in situ PCR,and multiple in situ PCR were carri...[Objective] The paper aimed to explore a simple in situ PCR technology for buckwheat.[Method] By using 16S and 4.5S nested primers and psbA primer,the in situ PCR,nested in situ PCR,and multiple in situ PCR were carried out on common buckwheat,respectively.[Result] High-temperature drying treatment had the effects similar to that of embedding method.The effect of the nested in situ PCR is better than conventional in situ PCR.A better result could be obtained till the multiple in situ PCR was performed as many as 5-6 times.Four pair of signals could be obtained by using both 16S and 4.5S primers,but their sites differed from each other;psbA primer as a single copy only showed a pair of signals.A total of five pairs of common buckwheat chromosome could be identified according to the difference of the signal＇s location.[Conclusion] The chromosome in situ PCR technique for buckwheat was simple and feasible.展开更多
基金Supported by the Earmarked Fund for China Agriculture Research System(CAES-08-A-4)Natural Science Foundation of China(31060207)+1 种基金Guizhou Key Agricultural Project(QianKeHe NY Zi[2010]3094)Guizhou Animal and Plant Breeding Project(Qian-NongYuZhuanZi[2010]023)~~
文摘[Objective] The paper aimed to explore a simple in situ PCR technology for buckwheat.[Method] By using 16S and 4.5S nested primers and psbA primer,the in situ PCR,nested in situ PCR,and multiple in situ PCR were carried out on common buckwheat,respectively.[Result] High-temperature drying treatment had the effects similar to that of embedding method.The effect of the nested in situ PCR is better than conventional in situ PCR.A better result could be obtained till the multiple in situ PCR was performed as many as 5-6 times.Four pair of signals could be obtained by using both 16S and 4.5S primers,but their sites differed from each other;psbA primer as a single copy only showed a pair of signals.A total of five pairs of common buckwheat chromosome could be identified according to the difference of the signal＇s location.[Conclusion] The chromosome in situ PCR technique for buckwheat was simple and feasible.
