Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-...Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-enriched peptides.We used five different enzymatic treatments to prepare Se-enriched protein hydrolysates from dark meat.Among them,the trypsin-generated protein hydrolysate showed the highestα-glucosidase inhibition activity.It was then fractionated by ultrafiltration followed by RP-HPLC,and the sequences of Se-enriched peptide obtained were identified from the most active fraction by LC-MS/MS.Subsequently,KPLSeCPK was screened by in silico analyses and validated by theα-glucosidase activity inhibition experiment(IC_(50)=0.828 mM).Molecular docking and inhibition kinetics assay further demonstrated the interaction between KPLSeCPK andα-glucosidase,suggesting its potential as a novel hyperglycemic inhibitor.Moreover,KPLSeCPK lessened IR and boosted antioxidant capability of IR-HepG2 cells.Thus,KPLSeCPK provides a valuable reference for efficiently utilizing natural organic Se.展开更多
Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative eff...Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative effects on insulin resistance in insulin resistant HepG2(IR-HepG2)cells.PTP was obtained from partridge tea,then PTP-1,PTP-2 and PTP-3 were obtained after further purification.The polyphenol contents of PTP,PTP-1,PTP-2,PTP-3 were determined to be 15.9%,51.4%,42.51% and 22.74%,respectively.PTP and PTP-1 had similar compositions,and the contents of the main components of PTP-1 was higher than that of PTP.Structural compositions and contents of PTP-2 and PTP-3 differ significantly.Further analysis indicated that PTP failed to have significant hypoglycaemic activity,while other three polyphenols regulated the fat metabolism disorders caused by insulin resistance,thus improving the body’s utilization of blood glucose.In addition,the three polyphenols reversed the damage caused by insulin resistance in IR-HepG2 cells by up-regulating the activities of HK(200μg/mL PTP-1:2.8414 mol/min/mL)and PK(200μg/mL PTP-1:10.7204 U/L),increasing the activity of SOD and CAT and decreasing the level of MDA.The mRNA results showed that PTP-1,PTP-2 and PTP-3 could achieve hypoglycaemic efficacy in the body by regulating the IRS-1/PI3K/AKT signal pathway.In summary,our results suggested that the polyphenols of partridge tea could be a promising natural source for preventing and treating hyperglycemia.展开更多
基金funded by the National Natural Science Foundation of China(32360578).
文摘Se-enriched peptides serve various physiological functions and are crucial sources of Se.This study found the Se content of yellowfin tuna dark meat was 18.34 mg/kg(dry weight),making it an ideal natural source of Se-enriched peptides.We used five different enzymatic treatments to prepare Se-enriched protein hydrolysates from dark meat.Among them,the trypsin-generated protein hydrolysate showed the highestα-glucosidase inhibition activity.It was then fractionated by ultrafiltration followed by RP-HPLC,and the sequences of Se-enriched peptide obtained were identified from the most active fraction by LC-MS/MS.Subsequently,KPLSeCPK was screened by in silico analyses and validated by theα-glucosidase activity inhibition experiment(IC_(50)=0.828 mM).Molecular docking and inhibition kinetics assay further demonstrated the interaction between KPLSeCPK andα-glucosidase,suggesting its potential as a novel hyperglycemic inhibitor.Moreover,KPLSeCPK lessened IR and boosted antioxidant capability of IR-HepG2 cells.Thus,KPLSeCPK provides a valuable reference for efficiently utilizing natural organic Se.
基金supported by the National Natural Science Foundation of China(32072201,32372286)Natural Science Foundation of Guangdong Province(2023A1515011967,2023A1515012223)+2 种基金Shenzhen Science and Technology Program(JCYJ20230807094503006,JCYJ20230807094503007)Foshan City Science and Technology Tackling Special Project in Social Areas(2120001008478)National Natural Foundation of China(82374239).
文摘Partridge tea is a plant used as a speciality tea drink in Hainan Province,China.The aim of this study was to isolate polyphenols from partridge tea,investigate their main constituent compositions and ameliorative effects on insulin resistance in insulin resistant HepG2(IR-HepG2)cells.PTP was obtained from partridge tea,then PTP-1,PTP-2 and PTP-3 were obtained after further purification.The polyphenol contents of PTP,PTP-1,PTP-2,PTP-3 were determined to be 15.9%,51.4%,42.51% and 22.74%,respectively.PTP and PTP-1 had similar compositions,and the contents of the main components of PTP-1 was higher than that of PTP.Structural compositions and contents of PTP-2 and PTP-3 differ significantly.Further analysis indicated that PTP failed to have significant hypoglycaemic activity,while other three polyphenols regulated the fat metabolism disorders caused by insulin resistance,thus improving the body’s utilization of blood glucose.In addition,the three polyphenols reversed the damage caused by insulin resistance in IR-HepG2 cells by up-regulating the activities of HK(200μg/mL PTP-1:2.8414 mol/min/mL)and PK(200μg/mL PTP-1:10.7204 U/L),increasing the activity of SOD and CAT and decreasing the level of MDA.The mRNA results showed that PTP-1,PTP-2 and PTP-3 could achieve hypoglycaemic efficacy in the body by regulating the IRS-1/PI3K/AKT signal pathway.In summary,our results suggested that the polyphenols of partridge tea could be a promising natural source for preventing and treating hyperglycemia.
