Structural shape monitoring plays a vital role in the structural health monitoring systems.The inverse finite element method(iFEM)has been demonstrated to be a practical method of deformation reconstruction owing to i...Structural shape monitoring plays a vital role in the structural health monitoring systems.The inverse finite element method(iFEM)has been demonstrated to be a practical method of deformation reconstruction owing to its unique advantages.Current iFEM formulations have been applied to small deformation of structures based on the small-displacement assumption of linear theory.However,this assumption may be inapplicable to some structures with large displacements in practical applications.Therefore,geometric nonlinearity needs to be considered.In this study,to expand the practical utility of iFEM for large displacement monitoring,we propose a nonlinear iFEM algorithm based on a four-node inverse quadrilateral shell element iQS4.Taking the advantage of an iterative iFEM algorithm,a nonlinear response is linearized to compute the geometrically nonlinear deformation reconstruction,like the basic concept of nonlinear FE analysis.Several examples are solved to verify the proposed approach.It is demonstrated that large displacements can be accurately estimated even if the in-situ sensor data includes different levels of randomly generated noise.It is proven that the nonlinear iFEM algorithm provides a more accurate displacement response as compared to the linear iFEM methodology for structures undergoing large displacement.Hence,the proposed approach can be utilized as a viable tool to effectively characterize geometrically nonlinear deformations of structures in real-time applications.展开更多
目的探讨IQSEC1是否通过病毒蛋白PB1调控甲型流感病毒的增殖。方法首先克隆甲型流感病毒[A/Shanghai/02/2013(H7N9)]的8个基因;其次,通过免疫共沉淀检测IQ模体Sec7结构域蛋白1(IQSEC1)与聚合酶PB1(PB1)存在相互作用;此外,通过过表达或...目的探讨IQSEC1是否通过病毒蛋白PB1调控甲型流感病毒的增殖。方法首先克隆甲型流感病毒[A/Shanghai/02/2013(H7N9)]的8个基因;其次,通过免疫共沉淀检测IQ模体Sec7结构域蛋白1(IQSEC1)与聚合酶PB1(PB1)存在相互作用;此外,通过过表达或者敲低IQSEC1的方法检测IQSEC1对PB1核定位的影响;最后,过表达或者敲低IQSEC1后检测Influenza A virus[A/Shanghai/02/2013(H7N9)]。结果病毒感染条件下,外源IQSEC1和PB1存在相互作用。当过表达IQSEC1时,细胞中IQSEC1的表达量上升,相应的PB1在细胞核中的定位减少;当用敲低IQSEC1时,细胞中IQSEC1的表达量下降,相应的PB1在细胞核中的定位上升。过表达IQSEC1后,甲型流感病毒的增殖水平下降(P<0.05)。敲低IQSEC1后,甲型流感病毒的增殖水平上升(P<0.05)。结论IQSEC1通过减少甲型流感病毒蛋白PB1的核定位抑制甲型流感病毒的增殖。展开更多
基金supported by the NationalNatural Science Foundation of China(Grant No.11902253)the Fundamental Research Funds for the Central Universities of China.The authors are grateful for this support.
文摘Structural shape monitoring plays a vital role in the structural health monitoring systems.The inverse finite element method(iFEM)has been demonstrated to be a practical method of deformation reconstruction owing to its unique advantages.Current iFEM formulations have been applied to small deformation of structures based on the small-displacement assumption of linear theory.However,this assumption may be inapplicable to some structures with large displacements in practical applications.Therefore,geometric nonlinearity needs to be considered.In this study,to expand the practical utility of iFEM for large displacement monitoring,we propose a nonlinear iFEM algorithm based on a four-node inverse quadrilateral shell element iQS4.Taking the advantage of an iterative iFEM algorithm,a nonlinear response is linearized to compute the geometrically nonlinear deformation reconstruction,like the basic concept of nonlinear FE analysis.Several examples are solved to verify the proposed approach.It is demonstrated that large displacements can be accurately estimated even if the in-situ sensor data includes different levels of randomly generated noise.It is proven that the nonlinear iFEM algorithm provides a more accurate displacement response as compared to the linear iFEM methodology for structures undergoing large displacement.Hence,the proposed approach can be utilized as a viable tool to effectively characterize geometrically nonlinear deformations of structures in real-time applications.
文摘目的探讨IQSEC1是否通过病毒蛋白PB1调控甲型流感病毒的增殖。方法首先克隆甲型流感病毒[A/Shanghai/02/2013(H7N9)]的8个基因;其次,通过免疫共沉淀检测IQ模体Sec7结构域蛋白1(IQSEC1)与聚合酶PB1(PB1)存在相互作用;此外,通过过表达或者敲低IQSEC1的方法检测IQSEC1对PB1核定位的影响;最后,过表达或者敲低IQSEC1后检测Influenza A virus[A/Shanghai/02/2013(H7N9)]。结果病毒感染条件下,外源IQSEC1和PB1存在相互作用。当过表达IQSEC1时,细胞中IQSEC1的表达量上升,相应的PB1在细胞核中的定位减少;当用敲低IQSEC1时,细胞中IQSEC1的表达量下降,相应的PB1在细胞核中的定位上升。过表达IQSEC1后,甲型流感病毒的增殖水平下降(P<0.05)。敲低IQSEC1后,甲型流感病毒的增殖水平上升(P<0.05)。结论IQSEC1通过减少甲型流感病毒蛋白PB1的核定位抑制甲型流感病毒的增殖。
