IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学...IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学计划要求,根据教学大纲规定的内容和质量要求进行教学和考核,质量可控,满足现有招生学生全部的实践要求。展开更多
Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hai...Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.展开更多
Distributed certification via threshold cryptography is much more secu re than other ways to protect certification authority (CA) 's private key,and c an tolerate some intrusions. As the original system such as IT...Distributed certification via threshold cryptography is much more secu re than other ways to protect certification authority (CA) 's private key,and c an tolerate some intrusions. As the original system such as ITTC,etc.,is unsaf e ,inefficient and impracitcal in actual network environment,this paper brings u p a new distributed certification scheme,which although it generates key shares concentratively,it updates key shares distributedly,and so,avoids single-po in t failure like ITTC. It not only enhances robustness with Feldman verification and SSL protocol,but can also change the threshold (t,k) flexibly and robu stly,and so,is much more practical. In this work,the authors implement the prototype sy stem of the new scheme and test and analyze its performance.展开更多
文摘IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学计划要求,根据教学大纲规定的内容和质量要求进行教学和考核,质量可控,满足现有招生学生全部的实践要求。
文摘Objective:To investigate the biochemical capacity,and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L.(Sabz and Siah)on Leishmania major promastigotes and amastigotes.Methods:In the hairy roots,the activity of antioxidant enzymes compared to normal leaves and roots,and the presence of some phenolic compounds in comparison with fruits were investigated.The IC_(50) values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays.By calculating the infectivity index of peripheral blood mononuclear cells(PBMCs),the leishmanicidal activity(IC_(50) values)of hairy roots for amastigotes was estimated.The effects of hairy roots(IC_(50) values)treatment on the levels of IFN-γ and iNOS expression,intracellular reactive oxygen species,and iNOS protein expression in infected-PBMCs were determined.Results:Based on antioxidant enzyme assays and high performance liquid chromatography analysis,hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds.According to the results of tetrazolium-dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays,the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes.Depending on the concentration and exposure time,treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species,up-regulation of IFN-γ and iNOS genes expression,and high value of iNOS protein compared to controls.Conclusions:The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.
文摘Distributed certification via threshold cryptography is much more secu re than other ways to protect certification authority (CA) 's private key,and c an tolerate some intrusions. As the original system such as ITTC,etc.,is unsaf e ,inefficient and impracitcal in actual network environment,this paper brings u p a new distributed certification scheme,which although it generates key shares concentratively,it updates key shares distributedly,and so,avoids single-po in t failure like ITTC. It not only enhances robustness with Feldman verification and SSL protocol,but can also change the threshold (t,k) flexibly and robu stly,and so,is much more practical. In this work,the authors implement the prototype sy stem of the new scheme and test and analyze its performance.
