Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and r...Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2S^(v)chromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2S^(v)L using an F_(2)population.Two 2S^(v)-2B recombinants,derived from a cross of the 2S^(v)(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2S^(l)of the Ae.longissima(S^(v)genome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.展开更多
By measuring the contents of soil organic carbon(SOC),total nitrogen(TN),and total phosphorus(P)in rhizosphere and non-rhizosphere soils of Quercus variabilis forests with different ages(22,35,and 45 a)and origins(nat...By measuring the contents of soil organic carbon(SOC),total nitrogen(TN),and total phosphorus(P)in rhizosphere and non-rhizosphere soils of Quercus variabilis forests with different ages(22,35,and 45 a)and origins(natural forests and planted forests)in the Funiu Mountain area,the stoichiometric characteristics of carbon,nitrogen,and phosphorus in Q.variabilis forest soils were evaluated.The research results indicated that:(i)there were significant differences(P<0.05)in the SOC content between the rhizosphere and non-rhizosphere soils of Q.variabilis planted forests at different stand ages.Moreover,as the stand age increased,the SOC content in both the rhizosphere and non-rhizosphere soils of the planted forests initially decreased and then increased.The TN and TP contents in the rhizosphere and non-rhizosphere soils of Q.variabilis planted forests did not show significant differences across different stand ages,and they exhibited different trends as the stand age increased.(ii)The SOC and nitrogen to phosphorus ratio(N:P)exhibited significant rhizosphere effects,whereas the rhizosphere effects of the TN,TP,carbon to nitrogen ratio(C:N),and carbon to phosphorus ratio(C:P)were not prominent.In terms of the influence of stand age,the SOC,TN,TP,C:P,and N:P were significantly affected.However,the effect of the C:N at the stand age level was not significant.The interactive effects of soil carbon,nitrogen,phosphorus,and their stoichiometric ratios were all significant.It is recommended to appropriately supplement soil nitrogen and phosphorus to ensure the sustainable development of Q.variabilis forest stand.展开更多
基金supported by the National Natural Science Foundation of China(32172020,31971884)the National Key Research and Development Program of China(2024YFD1201202,2024YFD1200402)+4 种基金the Sichuan Science and Technology Program(2022ZDZX0014,2023YFN0085)the Qinghai Provincial Key Laboratory of Crop Molecular Breeding(2023-1-1)the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China(SKLKF202409)the Alliance of National and International Science Organizations for the Belt and Road Regions(ANSO-CR-KP-202205)the International Partnership Program of Chinese Academy of Sciences(077GJHZ2023028GC)。
文摘Aegilops variabilis(S^(v)S^(v)U^(v)U^(v))is a source of resistance to wheat stripe rust.The phKL locus in Chinese common wheat landrace Kaixian-Luohanmai(KL)can induce homoeologous wheat-alien chromosome pairing and recombination.In this study,we confirmed that the whole 2S^(v)chromosome introgressed into wheat from Ae.variabilis accession AS116 conferred all-stage stripe rust resistance.The underlying gene(s),named YrAev,was mapped to the long arm 2S^(v)L using an F_(2)population.Two 2S^(v)-2B recombinants,derived from a cross of the 2S^(v)(2B)chromosome substitution line and KL,were confirmed to harbor the resistance locus.The physical region containing YrAev,determined from RNA-seq data,was 844.6-852.1 Mb on the chromosome arm 2S^(l)of the Ae.longissima(S^(v)genome donor species of Ae.variabilis)accession TL05 assembly v1.0.Differential gene expression analysis of post-inoculation with the Pst race has indicated two disease-resistance-related genes(annotated as mixed lineage kinase domain-like protein and nucleotide-binding leucine-rich repeat like protein,respectively)as promising candidates for YrAev.This study demonstrates the utility of the phKL gene system in alien gene localization and transfer.The resistant translocation line harboring YrAev can be exploited by wheat breeders as a novel source of resistance to stripe rust.
基金Sponsored by the National Natural Science Foundation of China(32271848).
文摘By measuring the contents of soil organic carbon(SOC),total nitrogen(TN),and total phosphorus(P)in rhizosphere and non-rhizosphere soils of Quercus variabilis forests with different ages(22,35,and 45 a)and origins(natural forests and planted forests)in the Funiu Mountain area,the stoichiometric characteristics of carbon,nitrogen,and phosphorus in Q.variabilis forest soils were evaluated.The research results indicated that:(i)there were significant differences(P<0.05)in the SOC content between the rhizosphere and non-rhizosphere soils of Q.variabilis planted forests at different stand ages.Moreover,as the stand age increased,the SOC content in both the rhizosphere and non-rhizosphere soils of the planted forests initially decreased and then increased.The TN and TP contents in the rhizosphere and non-rhizosphere soils of Q.variabilis planted forests did not show significant differences across different stand ages,and they exhibited different trends as the stand age increased.(ii)The SOC and nitrogen to phosphorus ratio(N:P)exhibited significant rhizosphere effects,whereas the rhizosphere effects of the TN,TP,carbon to nitrogen ratio(C:N),and carbon to phosphorus ratio(C:P)were not prominent.In terms of the influence of stand age,the SOC,TN,TP,C:P,and N:P were significantly affected.However,the effect of the C:N at the stand age level was not significant.The interactive effects of soil carbon,nitrogen,phosphorus,and their stoichiometric ratios were all significant.It is recommended to appropriately supplement soil nitrogen and phosphorus to ensure the sustainable development of Q.variabilis forest stand.
