摘要
目的系统研究变异黄芪Astragalus variabilis的生物碱成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱、半制备高效液相色谱等分离技术对变异黄芪生物碱进行分离纯化,并通过波谱技术鉴定其化学结构。分别采用四氮唑盐还原法(tetrazolium salt reduction assay,MTT)和格里斯试剂法(griess reagent assay,Griess)评价化合物1的体外抗肿瘤活性及抗炎活性。结果从变异黄芪二氯甲烷部位中分离得到13个生物碱成分,分别鉴定为octahydrooxireno[a]indolizine N-oxide(1)、(1R,8aS)-1-hydroxy-indolizidines(2)、苦豆碱(3)、去氢骆驼蓬碱(4)、槐胺碱(5)、苦参碱(6)、槐果碱(7)、9α-羟基苦参碱(8)、氧化槐定碱(9)、鸭嘴花碱(10)、L-vasicine(11)、脱氧鸭嘴花碱(12)和鸭嘴花碱酮(13)。化合物1在浓度100μmol/L时,没有表现出对人肺癌A549细胞、人肝癌HepG2细胞及巨噬细胞(NO)的抑制作用。结论化合物1为1种新的吲哚里西啶类生物碱,命名为变异黄芪碱A(variabilismine A);化合物1~13为首次从变异黄芪中分离得到。
Objective To study the alkaloid constituents from Astragalus variabilis.Methods Alkaloids were isolated and purified using chromatographic techniques,including silica gel column chromatography,Sephadex LH-20 column chromatography,and semi-preparative high-performance liquid chromatography(HPLC).Their chemical structures were elucidated through comprehensive spectroscopic analyses.The in vitro antitumor and anti-inflammatory activities of compound 1 were evaluated using the tetrazolium salt reduction assay(MTT)and the griess reagent assay,respectively.Results A total of 13 alkaloids were isolated from the dichloromethane extraction of A.variabilis and identified as octahydrooxireno[a]indolizine N-oxide(1),(1R,8aS)-1-hydroxy-indolizidines(2),aloperine(3),harmine(4),sophoramine(5),matrine(6),sophocarpine(7),9α-hydroxymatrine(8),oxysophoridine(9),vasicine(10),L-vasicine(11),deoxyvasicine(12)and L-vasicinone(13).Compound 1 didn’t exhibit inhibitory effects against human lung cancer cell line A549,human hepatocellular carcinoma cell line HepG2,and nitric oxide(NO)production in macrophages,at 100μmol/L.Conclusion Compounds(1–13)were isolated from A.variabilis for the first time,while compound 1 is a new indolizidine alkaloid,designated as variabilismine A.
作者
谭晶
杨敏
杨孙缘
熊荣琴
陈仁俊
张雅昆
曾艳荣
张振
路浩
裴国顺
谭承建
TAN Jing;YANG Min;YANG Sunyuan;XIONG Rongqin;CHEN Renjun;ZHANG Yakun;ZENG Yanrong;ZHNAG Zhen;LU Hao;PEI Guoshun;TAN Chengjian(School of Ethnic-Minority Medicine,Guizhou Minzu University,Guiyang 550025,China;Guizhou Academy of Sciences,Guiyang 550025,China;College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China)
出处
《中草药》
北大核心
2025年第22期8069-8076,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(32160110)
国家自然科学基金项目(31660103)
贵州省研究生教育创新计划项目资助(2024YJSKYJJ225)
贵州省科技计划项目(黔科合基础-ZK[2022]一般201)。
