5月11日,在北京举行的2019北京国际摩托车展览会上,光阳摩托(KYMCO)举行了盛大的新品发布会,3款电摩陆续登场,可谓新品璀璨,熠熠生辉。这3款电摩分别是:电动重机超跑SuperNEX概念车,以及搭载'正弦波内转子'电机的专业级电动踏...5月11日,在北京举行的2019北京国际摩托车展览会上,光阳摩托(KYMCO)举行了盛大的新品发布会,3款电摩陆续登场,可谓新品璀璨,熠熠生辉。这3款电摩分别是:电动重机超跑SuperNEX概念车,以及搭载'正弦波内转子'电机的专业级电动踏板摩托i-Like EV系列的i-Like EV pro版和i-Like EV标准版。3款车的发布,标志着光阳于去年宣布进军新能源市场后,已进入首发旗舰机种即将上市的实质性发展阶段。展开更多
目的:急性肾损伤(acute kidney injury,AKI)是临床上常见的危重症,目前仍缺乏早期、精准且无创的诊断和治疗手段,亟需深入探讨AKI发生和发展的潜在机制,寻找更精准可靠的靶点。本研究探讨缺血/再灌注(ischemia/reperfusion,I/R)、顺铂(c...目的:急性肾损伤(acute kidney injury,AKI)是临床上常见的危重症,目前仍缺乏早期、精准且无创的诊断和治疗手段,亟需深入探讨AKI发生和发展的潜在机制,寻找更精准可靠的靶点。本研究探讨缺血/再灌注(ischemia/reperfusion,I/R)、顺铂(cisplatin,Cis)、叶酸(folic acid,FA)诱导的3种AKI小鼠模型中整合素样金属蛋白酶结构域含血小板反应蛋白I型基序样3(adisintegrin-like and metalloprotease domain with thrombospondin type I motifslike-3,ADAMTSL3)的表达水平与分布情况。方法:将24只雄性C57BL/6小鼠随机分为对照组(Sham)、I/R组、Cis组和FA组4组,每组6只。造模后检测血肌酐(serum creatinine,SCr)和血尿素氮(blood urea nitrogen,BUN)水平。取各组小鼠的肾脏组织进行制片,并进行苏木精-伊红(hematoxylin and eosin,HE)染色、过碘酸希夫(periodic acid Schiff,PAS)染色、马松三色(Masson’s trichrome,Masson)染色、原位末端转移酶标记(terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling,TUNEL)染色、免疫荧光染色及蛋白质印迹法(Western blotting,WB)观察肾脏的损伤情况及ADAMTSL3的表达水平。结果:与Sham组相比,I/R、Cis和FA组的SCr、BUN均显著升高(均P<0.05),肾小管损伤程度均更严重,胶原纤维化、糖原沉积及细胞凋亡均增加,ADAMTSL3蛋白的表达水平均显著升高(均P<0.05),且主要定位于肾小管和间质;同时,Cis与FA组中ADAMTSL3蛋白的表达水平均高于I/R组(均P<0.05)。结论:3种AKI小鼠模型均成功建立,各组AKI小鼠肾脏中ADAMTSL3蛋白的表达水平均显著升高,可能为损伤性表达,可以作为肾实质损伤的潜在定位标志。ADAMTSL3可能在AKI发生和发展的机制中发挥重要作用,靶向ADAMTSL3可能可以帮助开发早期、无创、精准的新型AKI诊断标志物和靶点药物。展开更多
AIM:To determine the role of epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3)in pathogenesis of hepatocellular carcinoma(HCC)by investigating the EDIL3 expression in HCC and its prognostic va...AIM:To determine the role of epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3)in pathogenesis of hepatocellular carcinoma(HCC)by investigating the EDIL3 expression in HCC and its prognostic value for HCC.METHODS:EDIL3 expression was detected in 101 HCC surgical tissue samples with immunohistochemistry method,and its relation with clinicopathologic features and prognosis of HCC patients was analyzed.RESULTS:EDIL3 was highly expressed in 48.5%of the HCC patients.Although the EDIL3 expression level did not correlate with any clinicopathological parameters,KaplanMeier survival analysis showed that high expression level of EDIL3 resulted in a significantly poor prognosis of HCC patients(log-rank test,P=0.010).Multivariate Cox's analysis showed that the EDIL3 expression level was a significant and independent prognostic parameter for the overall survival rate of HCC patients(hazard ratio=1.978,95%confidence interval =1.139-3.435,P=0.015).CONCLUSION:High expression level of EDIL3 pre-dicts poor prognosis of HCC patients.EDIL3 may be a potential target of antiangiogenic therapy for HCC.展开更多
文摘5月11日,在北京举行的2019北京国际摩托车展览会上,光阳摩托(KYMCO)举行了盛大的新品发布会,3款电摩陆续登场,可谓新品璀璨,熠熠生辉。这3款电摩分别是:电动重机超跑SuperNEX概念车,以及搭载'正弦波内转子'电机的专业级电动踏板摩托i-Like EV系列的i-Like EV pro版和i-Like EV标准版。3款车的发布,标志着光阳于去年宣布进军新能源市场后,已进入首发旗舰机种即将上市的实质性发展阶段。
文摘目的:急性肾损伤(acute kidney injury,AKI)是临床上常见的危重症,目前仍缺乏早期、精准且无创的诊断和治疗手段,亟需深入探讨AKI发生和发展的潜在机制,寻找更精准可靠的靶点。本研究探讨缺血/再灌注(ischemia/reperfusion,I/R)、顺铂(cisplatin,Cis)、叶酸(folic acid,FA)诱导的3种AKI小鼠模型中整合素样金属蛋白酶结构域含血小板反应蛋白I型基序样3(adisintegrin-like and metalloprotease domain with thrombospondin type I motifslike-3,ADAMTSL3)的表达水平与分布情况。方法:将24只雄性C57BL/6小鼠随机分为对照组(Sham)、I/R组、Cis组和FA组4组,每组6只。造模后检测血肌酐(serum creatinine,SCr)和血尿素氮(blood urea nitrogen,BUN)水平。取各组小鼠的肾脏组织进行制片,并进行苏木精-伊红(hematoxylin and eosin,HE)染色、过碘酸希夫(periodic acid Schiff,PAS)染色、马松三色(Masson’s trichrome,Masson)染色、原位末端转移酶标记(terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling,TUNEL)染色、免疫荧光染色及蛋白质印迹法(Western blotting,WB)观察肾脏的损伤情况及ADAMTSL3的表达水平。结果:与Sham组相比,I/R、Cis和FA组的SCr、BUN均显著升高(均P<0.05),肾小管损伤程度均更严重,胶原纤维化、糖原沉积及细胞凋亡均增加,ADAMTSL3蛋白的表达水平均显著升高(均P<0.05),且主要定位于肾小管和间质;同时,Cis与FA组中ADAMTSL3蛋白的表达水平均高于I/R组(均P<0.05)。结论:3种AKI小鼠模型均成功建立,各组AKI小鼠肾脏中ADAMTSL3蛋白的表达水平均显著升高,可能为损伤性表达,可以作为肾实质损伤的潜在定位标志。ADAMTSL3可能在AKI发生和发展的机制中发挥重要作用,靶向ADAMTSL3可能可以帮助开发早期、无创、精准的新型AKI诊断标志物和靶点药物。
基金Supported by National Natural Science Foundation of China,No.u0772002
文摘AIM:To determine the role of epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3)in pathogenesis of hepatocellular carcinoma(HCC)by investigating the EDIL3 expression in HCC and its prognostic value for HCC.METHODS:EDIL3 expression was detected in 101 HCC surgical tissue samples with immunohistochemistry method,and its relation with clinicopathologic features and prognosis of HCC patients was analyzed.RESULTS:EDIL3 was highly expressed in 48.5%of the HCC patients.Although the EDIL3 expression level did not correlate with any clinicopathological parameters,KaplanMeier survival analysis showed that high expression level of EDIL3 resulted in a significantly poor prognosis of HCC patients(log-rank test,P=0.010).Multivariate Cox's analysis showed that the EDIL3 expression level was a significant and independent prognostic parameter for the overall survival rate of HCC patients(hazard ratio=1.978,95%confidence interval =1.139-3.435,P=0.015).CONCLUSION:High expression level of EDIL3 pre-dicts poor prognosis of HCC patients.EDIL3 may be a potential target of antiangiogenic therapy for HCC.
