The complexity and ehronicity of parasitic infections have obscured the identification of biologically relevant antigens.Analysis of the T cell receptor repertoire used by mice infected with leishmania major revealed ...The complexity and ehronicity of parasitic infections have obscured the identification of biologically relevant antigens.Analysis of the T cell receptor repertoire used by mice infected with leishmania major revealed the expansion of a restricted population of CD4+ cells.These cells expressed the Vα 8-JαTA72,Vβ4 heterodimer in both progressive infection and protective immunity and across several major histocompatibility haplotypes.Thus,the same immunodominant parasite epitope drives the disparate outcomes of this infectious process, suggesting that candidate vaccine antigens selected by screening of immune individuals may be capable of exacerbating disease in genetically susceptible individuals.展开更多
<strong>Objective:</strong> To explore the relationship between heat shock protein-90<i>α</i> (HSP-90<i>α</i>) and occurrence of prostate cancer, and clinical value of combined de...<strong>Objective:</strong> To explore the relationship between heat shock protein-90<i>α</i> (HSP-90<i>α</i>) and occurrence of prostate cancer, and clinical value of combined detection of serum HSP-90<i>α</i> and prostate specific antigen (PSA) in the diagnosis of prostate cancer. <strong>Method:</strong> A total of 30 patients with prostate cancer, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy men (control group) were selected from September 2018 to September 2019, then to detect levels of serum HSP-90<i>α</i>, total PSA and free PSA (FPSA) by ELISA, serum testosterone level by radioimmunoassay, prostate cancer tissue was removed by operation, and relative expression of tissue HSP-90<i>α</i> protein by Western blot. <strong>Results:</strong> The levels of serum HSP-90<i>α</i> and total PSA in prostate cancer group were significantly higher than other two groups, and testosterone level was lower than other two groups (P < 0.05);there was no difference of serum FPSA level between the three groups (P > 0.05). It was found by Pearson test that serum HSP-90<i>α</i> was positively correlated with total PSA level (r = 0.659, P = 0.005), while negatively correlated with testosterone level (r = -0.549, P = 0.006). According to TNM stage of prostate cancer, there were 17 cases of stage I - II, 13 cases of stage III - IV, 6 cases of Gleason score 1 - 4, 13 cases of 5 - 7, 11 cases of 8 - 10, tumor diameter range from 0.8 to 6.2 cm, with average of (3.9 ± 1.5) cm. The relative expression of HSP-90<i>α</i> protein in tumor tissue was closely related to TNM stage, Gleason score and tumor diameter (P < 0.05). By ROC analysis, it was found that accuracy of combined detection of serum HSP-90<i>α</i> and PSA levels for prostate cancer diagnosis was 0.896, and that of single PSA detection was 0.852. <strong>Conclusion:</strong> Higher expressions of HSP-90<i>α</i> in prostate cancer tissue and serum may be closely related to occurrence and development of prostate cancer, and combined detections of serum HSP-90<i>α</i> and PSA levels are of great significance in improving early diagnosis of prostate cancer.展开更多
Blastomycosis, the systemic fungal disease of humans and animals caused by <i>Blastomyces dermatitidis </i>and the cryptic species <i>Blastomyces gilchristii</i><span>,<i> </i>...Blastomycosis, the systemic fungal disease of humans and animals caused by <i>Blastomyces dermatitidis </i>and the cryptic species <i>Blastomyces gilchristii</i><span>,<i> </i></span>is often misdiagnosed as a bacterial or viral pulmonary disease. Therefore, the development of improved immunodiagnostic assays for this disease has been the primary focus of research in our laboratory. The present study was designed to evaluate four <span>Blastomyces</span> yeast-phase lysate antigenic preparations (human, 597, Eagle River, WI;dog, ERC-2, WI;Human, B5927, Mountain Iron, MN;soil, 85, Georgia, ATCC 56920) for their ability to detect antibody in 48 serum specimens from dogs with diagnosed blastomycosis using an indirect ELISA (STD) compared to a biotin-streptavidin ELISA (B-SA). All four lysate antigens were able to detect antibod<span style="font-family:;" "="">ies</span><span style="font-family:;" "=""> in the specimens with mean absorbance values ranging from 0.930 (B5927) to 1.142 (ERC-2) with the STD ELSA and from 1.395 (B5927) to 1.775 (85) with the B-SA ELISA. The results indicated that both ELISA methods could be utilized for antibody detection, but the B-SA ELISA exhibited greater sensitivity than the STD ELISA with all four of the lysates.</span>展开更多
文摘The complexity and ehronicity of parasitic infections have obscured the identification of biologically relevant antigens.Analysis of the T cell receptor repertoire used by mice infected with leishmania major revealed the expansion of a restricted population of CD4+ cells.These cells expressed the Vα 8-JαTA72,Vβ4 heterodimer in both progressive infection and protective immunity and across several major histocompatibility haplotypes.Thus,the same immunodominant parasite epitope drives the disparate outcomes of this infectious process, suggesting that candidate vaccine antigens selected by screening of immune individuals may be capable of exacerbating disease in genetically susceptible individuals.
文摘<strong>Objective:</strong> To explore the relationship between heat shock protein-90<i>α</i> (HSP-90<i>α</i>) and occurrence of prostate cancer, and clinical value of combined detection of serum HSP-90<i>α</i> and prostate specific antigen (PSA) in the diagnosis of prostate cancer. <strong>Method:</strong> A total of 30 patients with prostate cancer, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy men (control group) were selected from September 2018 to September 2019, then to detect levels of serum HSP-90<i>α</i>, total PSA and free PSA (FPSA) by ELISA, serum testosterone level by radioimmunoassay, prostate cancer tissue was removed by operation, and relative expression of tissue HSP-90<i>α</i> protein by Western blot. <strong>Results:</strong> The levels of serum HSP-90<i>α</i> and total PSA in prostate cancer group were significantly higher than other two groups, and testosterone level was lower than other two groups (P < 0.05);there was no difference of serum FPSA level between the three groups (P > 0.05). It was found by Pearson test that serum HSP-90<i>α</i> was positively correlated with total PSA level (r = 0.659, P = 0.005), while negatively correlated with testosterone level (r = -0.549, P = 0.006). According to TNM stage of prostate cancer, there were 17 cases of stage I - II, 13 cases of stage III - IV, 6 cases of Gleason score 1 - 4, 13 cases of 5 - 7, 11 cases of 8 - 10, tumor diameter range from 0.8 to 6.2 cm, with average of (3.9 ± 1.5) cm. The relative expression of HSP-90<i>α</i> protein in tumor tissue was closely related to TNM stage, Gleason score and tumor diameter (P < 0.05). By ROC analysis, it was found that accuracy of combined detection of serum HSP-90<i>α</i> and PSA levels for prostate cancer diagnosis was 0.896, and that of single PSA detection was 0.852. <strong>Conclusion:</strong> Higher expressions of HSP-90<i>α</i> in prostate cancer tissue and serum may be closely related to occurrence and development of prostate cancer, and combined detections of serum HSP-90<i>α</i> and PSA levels are of great significance in improving early diagnosis of prostate cancer.
文摘Blastomycosis, the systemic fungal disease of humans and animals caused by <i>Blastomyces dermatitidis </i>and the cryptic species <i>Blastomyces gilchristii</i><span>,<i> </i></span>is often misdiagnosed as a bacterial or viral pulmonary disease. Therefore, the development of improved immunodiagnostic assays for this disease has been the primary focus of research in our laboratory. The present study was designed to evaluate four <span>Blastomyces</span> yeast-phase lysate antigenic preparations (human, 597, Eagle River, WI;dog, ERC-2, WI;Human, B5927, Mountain Iron, MN;soil, 85, Georgia, ATCC 56920) for their ability to detect antibody in 48 serum specimens from dogs with diagnosed blastomycosis using an indirect ELISA (STD) compared to a biotin-streptavidin ELISA (B-SA). All four lysate antigens were able to detect antibod<span style="font-family:;" "="">ies</span><span style="font-family:;" "=""> in the specimens with mean absorbance values ranging from 0.930 (B5927) to 1.142 (ERC-2) with the STD ELSA and from 1.395 (B5927) to 1.775 (85) with the B-SA ELISA. The results indicated that both ELISA methods could be utilized for antibody detection, but the B-SA ELISA exhibited greater sensitivity than the STD ELISA with all four of the lysates.</span>
