The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administrati...The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administration with 50-- 200 mg/kg body weight of PEH to type II diabetic mice induced by alloxan for four weeks, the blood glucose level of mice was decreased significantly. Moreover, the SOD activity increased at the same time（p〈0.01）. PEH showed a significant anti-diabetic and anti-oxidation activity. In addition, PEH affected the BUN and ALT levels（p〈0.05）, which are important characteristics of diabetes mellitus. Through this work, PEH should be a potential anti-diabetic drug in the future.展开更多
Huidouba is a kind of fungus plant that grows by spiders parasitizing tea trees at an altitude of 1500 m above Mount Emei.The bioactive polysaccharides extracted from Huidouba have been traditionally used in the treat...Huidouba is a kind of fungus plant that grows by spiders parasitizing tea trees at an altitude of 1500 m above Mount Emei.The bioactive polysaccharides extracted from Huidouba have been traditionally used in the treatment of diabetes in China.In this study,the optimal hot-water extraction condition of crude Huidouba polysaccharides was obtained using Box-Behnken design:the temperature of 93.7◦C,the time of 3.57 h,the liquid-to-solid ratio of 28.47 mL/g,and the yield was 0.46%.Three purified Huidouba polysaccharides(HDBPs,including HDBP-d,HDBP-1,and HDBP-2)were separated through deproteinization,ultrafiltration,and column chromatography.Then the inhibition mechanism and kinetics of HDBPs againstα-glucosidase were investigated.The inhibitory activity of HDBPs onα-glucosidase was positively correlated with their concentration,and their mechanism of quenching fluorescence was ascertained to be the static type induced by the formation of HDBPs-α-glucosidase complex.HDBP-d and HDBP-2 inhibited the activity ofα-glucosidase in a reversible competitive-type manner while HDBP-1 was a noncompetitive-type inhibitor.The inhibitory activities of HDBPs onα-glucosidase correlated inversely with temperature in most cases,and showed weaker inhibitory activity at extreme acidic or alkaline conditions.These findings highlight the efficacy,kinetics and mechanism of HDBPs inhibitingα-glucosidase in vitro.The study provided a replaceable approach for chemical inhibitors ofα-glucosidase with natural fungus plant extracts,and suggested the theoretical basis that the plant-derived polysaccharides with glucosidase inhibition effects were applied in medicines.展开更多
Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB tre...Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB treatment could enhance glucose tolerance and insulin sensitivity,and thus prevent a hyperglycemia state.Methods:To test the hypothesis,streptozotocin(STZ)-induced diabetic mice and db/db mice,widely used models of hyperglycemia and insulin-resistant diabetes,were either treated with HDB,metformin,or acarbose.Blood glucose,oral glucose tolerance test,insulin tolerance test,pancreatic histopathology and serum biochemistry were detected to assess the hypoglycemic effect of HDB.Results:HDB treatments were found to show the effect in reducing glucose levels.HDB also resulted in a significant reduction in body weight and food intake in the STZ-induced diabetic mouse model.Furthermore,it significantly improved glucose and insulin tolerance in the two diabetic mouse models.Importantly,insulin,glucagon,pancreatic polypeptide,and somatostatin immunohistochemistry revealed that HDB treatment improved the function and the location of the cells in the islets compared with the other two treatments.HDB treatment resulted in significant restoration of islet function.Our results illustrated the underlying mechanism of HDB in the progression of diabetes,and HDB can be an effective agent for the treatment of diabetes.Conclusion:The results of this study suggested that HDB can reduce blood glucose levels in STZ-induced hyperglycemic mice and db/db mice.展开更多
基金Supported by the China Medical Foundation(No.20061108)
文摘The polysaccharide extracted from Huidouba（PEH） exihibited a significant anti-diabetic activity. The polysaccharide contained glucose, mannose and galactose in a content ratio of 1.00：0.34：0.32. After administration with 50-- 200 mg/kg body weight of PEH to type II diabetic mice induced by alloxan for four weeks, the blood glucose level of mice was decreased significantly. Moreover, the SOD activity increased at the same time（p〈0.01）. PEH showed a significant anti-diabetic and anti-oxidation activity. In addition, PEH affected the BUN and ALT levels（p〈0.05）, which are important characteristics of diabetes mellitus. Through this work, PEH should be a potential anti-diabetic drug in the future.
基金supported by the National Key R&D Program of China(no.2016YFD0400303)Guangdong Province YangFan Innovative and Entrepreneurial Research Team Project(no.2014YT02S029).
文摘Huidouba is a kind of fungus plant that grows by spiders parasitizing tea trees at an altitude of 1500 m above Mount Emei.The bioactive polysaccharides extracted from Huidouba have been traditionally used in the treatment of diabetes in China.In this study,the optimal hot-water extraction condition of crude Huidouba polysaccharides was obtained using Box-Behnken design:the temperature of 93.7◦C,the time of 3.57 h,the liquid-to-solid ratio of 28.47 mL/g,and the yield was 0.46%.Three purified Huidouba polysaccharides(HDBPs,including HDBP-d,HDBP-1,and HDBP-2)were separated through deproteinization,ultrafiltration,and column chromatography.Then the inhibition mechanism and kinetics of HDBPs againstα-glucosidase were investigated.The inhibitory activity of HDBPs onα-glucosidase was positively correlated with their concentration,and their mechanism of quenching fluorescence was ascertained to be the static type induced by the formation of HDBPs-α-glucosidase complex.HDBP-d and HDBP-2 inhibited the activity ofα-glucosidase in a reversible competitive-type manner while HDBP-1 was a noncompetitive-type inhibitor.The inhibitory activities of HDBPs onα-glucosidase correlated inversely with temperature in most cases,and showed weaker inhibitory activity at extreme acidic or alkaline conditions.These findings highlight the efficacy,kinetics and mechanism of HDBPs inhibitingα-glucosidase in vitro.The study provided a replaceable approach for chemical inhibitors ofα-glucosidase with natural fungus plant extracts,and suggested the theoretical basis that the plant-derived polysaccharides with glucosidase inhibition effects were applied in medicines.
基金supported by the National Natural Science Foundation of China (No.81673957)State Key Laboratory of Natural and Biomimetic Drug (No.K20160210)+1 种基金Independent Research Projects for Ph.D.Students of Minzu University of China (No.181084)Undergraduate Research and Training Program (URTP) of Minzu University of China (No.GCCX2020110018)。
文摘Objective:Huidouba(HDB) is a Chinese folk medicine used to treat diabetes in Sichuan Province,China.Therefore,we investigated the anti-diabetic effects of HDB and its underlying mechanisms.We hypothesized that HDB treatment could enhance glucose tolerance and insulin sensitivity,and thus prevent a hyperglycemia state.Methods:To test the hypothesis,streptozotocin(STZ)-induced diabetic mice and db/db mice,widely used models of hyperglycemia and insulin-resistant diabetes,were either treated with HDB,metformin,or acarbose.Blood glucose,oral glucose tolerance test,insulin tolerance test,pancreatic histopathology and serum biochemistry were detected to assess the hypoglycemic effect of HDB.Results:HDB treatments were found to show the effect in reducing glucose levels.HDB also resulted in a significant reduction in body weight and food intake in the STZ-induced diabetic mouse model.Furthermore,it significantly improved glucose and insulin tolerance in the two diabetic mouse models.Importantly,insulin,glucagon,pancreatic polypeptide,and somatostatin immunohistochemistry revealed that HDB treatment improved the function and the location of the cells in the islets compared with the other two treatments.HDB treatment resulted in significant restoration of islet function.Our results illustrated the underlying mechanism of HDB in the progression of diabetes,and HDB can be an effective agent for the treatment of diabetes.Conclusion:The results of this study suggested that HDB can reduce blood glucose levels in STZ-induced hyperglycemic mice and db/db mice.
