Objective:To study the mechanism of action of Yanghe Huayan Decoction on endocrine-resistant cells(MCF-7R/mTOR cells)with low expression of mTOR;Methods:CCK-8 assay and cell clone assay were used to detect cell prolif...Objective:To study the mechanism of action of Yanghe Huayan Decoction on endocrine-resistant cells(MCF-7R/mTOR cells)with low expression of mTOR;Methods:CCK-8 assay and cell clone assay were used to detect cell proliferation and clonal ability,and flow cytometry was used to detect cell apoptosis.The changes of cytokines in ER-PI3K/Akt/mTOR signaling pathway were analyzed by blot and QPCR.Results:Yanghe Huayan Decoction could significantly affect the proliferation(p<0.05)and cloning ability(p<0.05)of MCF-7R/mTOR cells,promote cell apoptosis(p<0.01),and downregulate the expression of ER,PI3K,AKT,mTOR and p-mTOR(p<0.05).Conclusion:Yanghe Huayan Decoction can regulate the ER-PI3K/Akt/mTOR signaling pathway with multiple targets,and the use of combined mTOR inhibitors can regulate the ER-PI3K/Akt/mTOR signaling pathway more significantly.展开更多
In the context of today’s coexistence of multi-religious traditions,how different religious traditions get along is not only an academic issue,but also a practical one.Huayan Buddhism is one of the eight major school...In the context of today’s coexistence of multi-religious traditions,how different religious traditions get along is not only an academic issue,but also a practical one.Huayan Buddhism is one of the eight major schools of Buddhism in China.The characteristic theory of Chinese Huayan Buddhism-fusion thought,providing a way of thinking and practical guidance for friendly dialogue between religions,has not only solved the problem of religious dialogue,but also promoted the development of harmonious relations among different religions.In addition,it has positive implications for exchanges between civilizations and cultural diversity.展开更多
基金National Natural Science Foundation of China(No.81573989)
文摘Objective:To study the mechanism of action of Yanghe Huayan Decoction on endocrine-resistant cells(MCF-7R/mTOR cells)with low expression of mTOR;Methods:CCK-8 assay and cell clone assay were used to detect cell proliferation and clonal ability,and flow cytometry was used to detect cell apoptosis.The changes of cytokines in ER-PI3K/Akt/mTOR signaling pathway were analyzed by blot and QPCR.Results:Yanghe Huayan Decoction could significantly affect the proliferation(p<0.05)and cloning ability(p<0.05)of MCF-7R/mTOR cells,promote cell apoptosis(p<0.01),and downregulate the expression of ER,PI3K,AKT,mTOR and p-mTOR(p<0.05).Conclusion:Yanghe Huayan Decoction can regulate the ER-PI3K/Akt/mTOR signaling pathway with multiple targets,and the use of combined mTOR inhibitors can regulate the ER-PI3K/Akt/mTOR signaling pathway more significantly.
文摘In the context of today’s coexistence of multi-religious traditions,how different religious traditions get along is not only an academic issue,but also a practical one.Huayan Buddhism is one of the eight major schools of Buddhism in China.The characteristic theory of Chinese Huayan Buddhism-fusion thought,providing a way of thinking and practical guidance for friendly dialogue between religions,has not only solved the problem of religious dialogue,but also promoted the development of harmonious relations among different religions.In addition,it has positive implications for exchanges between civilizations and cultural diversity.
基金陝西省社科基金項目“唐武周敦煌大族家窟莫高窟第332窟研究”(2023G003)陝西省石窟寺保護研究中心開放課題“唐代長安對敦煌石窟影響研究”(C2021—003)高等學校學科創新引智基地計劃資助(Supported by the Project 111)“長安與絲路文化傳播學科創新引智基地”(B18032)之階段性成果。
