Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO2 extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of...Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO2 extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 °C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield fla- vonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.展开更多
Objective:To systematically evaluate the protective effects of Humulus lupulus L.extract(HLE)on osteoporosis mice.Methods:In vivo experiment,a total of 3512-week-old female ICR mice were equally divided into 5 groups:...Objective:To systematically evaluate the protective effects of Humulus lupulus L.extract(HLE)on osteoporosis mice.Methods:In vivo experiment,a total of 3512-week-old female ICR mice were equally divided into 5 groups:the sham control group(sham);the ovariectomy with vehicle group(OVX);the OVX with estradiol valerate[EV,0.2 mg/(kg·d)];the OVX with low-or high-dose HLE groups[HLE,1 g/(kg·d)and 3 g/(kg·d)],7 in each group.Treatment began 1 week after the ovariectomized surgery and lasted for 12 weeks.Bone mass and trabecular bone mircoarchitecture were evaluated by micro computed tomography,and bone turnover markers in serum were evaluated using enzyme-linked immunosorbent assay(ELISA)kits.In vitro experiment,osteoblasts and osteoclasts were treated with HLE at doses of 0,4,20 and 100μg/mL.Biomarkers for bone formation in osteoblasts and bone resorption in osteoclasts were analyzed.Results:Compared with the OVX group,HLE exerted bone protective effects by the increase of estradiol(P<0.05),the improvement of cancellous bone structure,bone mineral density(P<0.01)and the reduction of serum alkaline phosphatase(ALP),tartrate resistant acid phosphatase(TRAP),bone gla-protein,c-terminal telopeptides of typeⅠcollagen(CTX-Ⅰ)and deoxypyridinoline levels(P<0.01 for all).In vitro experiment,compared with the control group,HLE at 20μg/mL promoted the cell proliferation(P<0.01),and increased the expression of bone morphogenetic protein-2 and osteopontin levels in osteoblasts(both P<0.05).HLE at 100μg/mL increased the osteoblastic ALP activities,and HLE at all dose enhanced the extracellular matrix mineralization(both P<0.01).Furthermore,compared with the control group,HLE at 20μg/m L and 100μg/m L inhibited osteoclastic TRAP activity(P<0.01),and reduced the expression of matrix metalloproteinase-9 and cathepsin K(both P<0.05).Conclusion:HLE may protect against bone loss,and have potentials in the treatment of osteoporosis.展开更多
Objective:To investigate the impact of Humulus Lupulus L.hydroalcoholic extract on the body weights,reproductive organs,sperm quality and hormone levels in male rats.Methods:By simple random sampling method,seventy ma...Objective:To investigate the impact of Humulus Lupulus L.hydroalcoholic extract on the body weights,reproductive organs,sperm quality and hormone levels in male rats.Methods:By simple random sampling method,seventy male Sprague-Dawley rats were randomly assigned to 7 groups including control group[distilled water,1 mL/(kg·d)],Tween 80 group[25%Tween 80 solution,1 mL/(kg·d)],olive oil group[olive oil,1 mL/(kg·d)],diethyl stilbestrol(DES)group[DES,100μg/(kg·body weight)],H50,H150 and H450[50,150 and 400 mg/(kg·d)of Humulus Lupulus L extract,respectively].The administration was performed via gavage once daily for 7 weeks.Body and reproductive organs weights including testes,seminal vesicles,epididymis and prostate were weighted and epididymal sperm quality were determined by digital balance.Blood samples were collected and serum free testosterone(T),luteinizing hormone(LH),follicle-stimulating hormone(FSH),and estrogen(E2)levels were measured by rat specific enzyme-linked immunosorbent assay.Results:The percentage increase in mean body weights of rats in the DES and H50,H150 and H450 groups decreased significantly compared to olive oil and Tween 80 groups(all P<0.05).The weights of seminal vesicle,epididymis and testes in rats receiving H50 were significantly higher than the control group(P<0.05 or P<0.01).The sperm count in the rats receiving H50 was significantly lower than the control group(P<0.05).The sperm motile characteristics of the rats receiving hydroalcoholic extract and DES were significantly lower than those of the control or rats receiving vehicles(all P<0.05).In H50,H150,H450 and DES groups,T and LH levels were decreased,and E2 was significantly increased compared to the control(P<0.05 or P<0.01).The FSH level did not change in all groups(P>0.05).Conclusion:Humulus Lupulus L.extract significantly increased the seminal vesicle and testes weights and reduced the sperm motility.展开更多
本研究以13个啤酒花品种(品系)为试材,对其茎尖培养的基本培养基及植物生长调节剂种类、浓度进行了研究。结果表明,各品种间在增殖数目和嫩茎高度上均存在差异;啤酒花的茎尖培养要求基本培养基中高的无机盐含量,MS.Anderson 培养基适于...本研究以13个啤酒花品种(品系)为试材,对其茎尖培养的基本培养基及植物生长调节剂种类、浓度进行了研究。结果表明,各品种间在增殖数目和嫩茎高度上均存在差异;啤酒花的茎尖培养要求基本培养基中高的无机盐含量,MS.Anderson 培养基适于啤酒花的茎尖培养。在植物生长调节剂的种类与浓度上,BA2.0mg·L^(-1)为各品种所必需;而对于 GA 及生长素的要求则因品种而异。卡斯凯特、簇生约基玛、巴卡、泰特纳吉不必添加 IBA 和 GA,8301,目标,布林、弗哥日需添加 GA0.3mg·L^(-1),威拉梅特、美国大花、柯密特需添加 IBA0.2mg·L^(-1),GA0.3mg·L^(-1),而赫斯布鲁克 G 则要求添加 IBA 2mg·L^(-1),GA0.3mg·L^(-1),青岛大花只需添加IBA2.0mg·L^(-1)。使用自来水代替蒸馏水取得良好结果。以微型扦插方式生根取得85%的生根株率,平均生根系数5,平均根长3.5cm,移栽成活率76%。展开更多
基金Project (No. Y304203) supported by the Natural Science Foundationof Zhejiang Province, China
文摘Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO2 extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 °C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield fla- vonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.
基金Supported by the National Natural Science Foundation of China(No.U1603283)。
文摘Objective:To systematically evaluate the protective effects of Humulus lupulus L.extract(HLE)on osteoporosis mice.Methods:In vivo experiment,a total of 3512-week-old female ICR mice were equally divided into 5 groups:the sham control group(sham);the ovariectomy with vehicle group(OVX);the OVX with estradiol valerate[EV,0.2 mg/(kg·d)];the OVX with low-or high-dose HLE groups[HLE,1 g/(kg·d)and 3 g/(kg·d)],7 in each group.Treatment began 1 week after the ovariectomized surgery and lasted for 12 weeks.Bone mass and trabecular bone mircoarchitecture were evaluated by micro computed tomography,and bone turnover markers in serum were evaluated using enzyme-linked immunosorbent assay(ELISA)kits.In vitro experiment,osteoblasts and osteoclasts were treated with HLE at doses of 0,4,20 and 100μg/mL.Biomarkers for bone formation in osteoblasts and bone resorption in osteoclasts were analyzed.Results:Compared with the OVX group,HLE exerted bone protective effects by the increase of estradiol(P<0.05),the improvement of cancellous bone structure,bone mineral density(P<0.01)and the reduction of serum alkaline phosphatase(ALP),tartrate resistant acid phosphatase(TRAP),bone gla-protein,c-terminal telopeptides of typeⅠcollagen(CTX-Ⅰ)and deoxypyridinoline levels(P<0.01 for all).In vitro experiment,compared with the control group,HLE at 20μg/mL promoted the cell proliferation(P<0.01),and increased the expression of bone morphogenetic protein-2 and osteopontin levels in osteoblasts(both P<0.05).HLE at 100μg/mL increased the osteoblastic ALP activities,and HLE at all dose enhanced the extracellular matrix mineralization(both P<0.01).Furthermore,compared with the control group,HLE at 20μg/m L and 100μg/m L inhibited osteoclastic TRAP activity(P<0.01),and reduced the expression of matrix metalloproteinase-9 and cathepsin K(both P<0.05).Conclusion:HLE may protect against bone loss,and have potentials in the treatment of osteoporosis.
基金Supported by the Research Affairs of Shiraz University of Medical Sciences(No.3001)
文摘Objective:To investigate the impact of Humulus Lupulus L.hydroalcoholic extract on the body weights,reproductive organs,sperm quality and hormone levels in male rats.Methods:By simple random sampling method,seventy male Sprague-Dawley rats were randomly assigned to 7 groups including control group[distilled water,1 mL/(kg·d)],Tween 80 group[25%Tween 80 solution,1 mL/(kg·d)],olive oil group[olive oil,1 mL/(kg·d)],diethyl stilbestrol(DES)group[DES,100μg/(kg·body weight)],H50,H150 and H450[50,150 and 400 mg/(kg·d)of Humulus Lupulus L extract,respectively].The administration was performed via gavage once daily for 7 weeks.Body and reproductive organs weights including testes,seminal vesicles,epididymis and prostate were weighted and epididymal sperm quality were determined by digital balance.Blood samples were collected and serum free testosterone(T),luteinizing hormone(LH),follicle-stimulating hormone(FSH),and estrogen(E2)levels were measured by rat specific enzyme-linked immunosorbent assay.Results:The percentage increase in mean body weights of rats in the DES and H50,H150 and H450 groups decreased significantly compared to olive oil and Tween 80 groups(all P<0.05).The weights of seminal vesicle,epididymis and testes in rats receiving H50 were significantly higher than the control group(P<0.05 or P<0.01).The sperm count in the rats receiving H50 was significantly lower than the control group(P<0.05).The sperm motile characteristics of the rats receiving hydroalcoholic extract and DES were significantly lower than those of the control or rats receiving vehicles(all P<0.05).In H50,H150,H450 and DES groups,T and LH levels were decreased,and E2 was significantly increased compared to the control(P<0.05 or P<0.01).The FSH level did not change in all groups(P>0.05).Conclusion:Humulus Lupulus L.extract significantly increased the seminal vesicle and testes weights and reduced the sperm motility.
文摘本研究以13个啤酒花品种(品系)为试材,对其茎尖培养的基本培养基及植物生长调节剂种类、浓度进行了研究。结果表明,各品种间在增殖数目和嫩茎高度上均存在差异;啤酒花的茎尖培养要求基本培养基中高的无机盐含量,MS.Anderson 培养基适于啤酒花的茎尖培养。在植物生长调节剂的种类与浓度上,BA2.0mg·L^(-1)为各品种所必需;而对于 GA 及生长素的要求则因品种而异。卡斯凯特、簇生约基玛、巴卡、泰特纳吉不必添加 IBA 和 GA,8301,目标,布林、弗哥日需添加 GA0.3mg·L^(-1),威拉梅特、美国大花、柯密特需添加 IBA0.2mg·L^(-1),GA0.3mg·L^(-1),而赫斯布鲁克 G 则要求添加 IBA 2mg·L^(-1),GA0.3mg·L^(-1),青岛大花只需添加IBA2.0mg·L^(-1)。使用自来水代替蒸馏水取得良好结果。以微型扦插方式生根取得85%的生根株率,平均生根系数5,平均根长3.5cm,移栽成活率76%。
