Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm...Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, using THF-CH3CN-H2O-H3PO4 （30 ： 5： 125 ： 0. 1） as the mobile phase at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was 270 nm. Results The linear range of 2＂-O-rhamnosyl vitexin and vitexin were 0. 106 4 μg - 2. 1280 μg （ r =0. 999 1 ） and 0. 139 2μg - 2. 784 0 μg （ r =0. 999 3 ）, respectively. The average recoveries of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% （ RSD = 2.80%, n = 6） and 100.6% （ RSD = 2.84%, n = 6）, respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.展开更多
Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with differ...Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 days.The appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold storage.Meanwhile,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ed.Furthermore,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning process.It not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con trolled.Additionally,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and catechol.Conelusion:Hawthorn leaf extract significantly alleviated the browning of fresh-cut potato.It could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.展开更多
目的分析山楂叶研究现状和研究热点,为其进一步研究提供参考。方法检索中国知识资源总库(CNKI)、中国学术期刊数据库(万方数据)、中文科技期刊数据库(VIP)、Web of Science(WOS)1980年4月30日-2024年10月31日收录的山楂叶研究文献,采用N...目的分析山楂叶研究现状和研究热点,为其进一步研究提供参考。方法检索中国知识资源总库(CNKI)、中国学术期刊数据库(万方数据)、中文科技期刊数据库(VIP)、Web of Science(WOS)1980年4月30日-2024年10月31日收录的山楂叶研究文献,采用NoteExpress4.0.0.9855软件进行文献管理和筛选,利用CiteSpace6.2.R6软件对作者、机构、国家和关键词进行可视化分析。结果共纳入中文文献793篇、英文文献122篇,中文文献发文趋势先上升后下降,英文文献发文趋势相对平稳。作者间有一定合作,且形成了团队间、机构间、跨国交流合作,但合作不够密切,中文发文最多的作者为潘海峰,英文发文最多的作者为Li,Yanrong(李艳荣);发文最多的机构为承德医学院。关键词分析显示,化学成分研究以黄酮、多酚、牧荆素、原花青素为主,药理作用研究以糖尿病、高脂血症、抗氧化作用、抗炎为主。结论山楂叶研究仍处于发展上升阶段,山楂叶配方颗粒深度研发可能为今后研究趋势。展开更多
文摘Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, using THF-CH3CN-H2O-H3PO4 （30 ： 5： 125 ： 0. 1） as the mobile phase at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was 270 nm. Results The linear range of 2＂-O-rhamnosyl vitexin and vitexin were 0. 106 4 μg - 2. 1280 μg （ r =0. 999 1 ） and 0. 139 2μg - 2. 784 0 μg （ r =0. 999 3 ）, respectively. The average recoveries of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% （ RSD = 2.80%, n = 6） and 100.6% （ RSD = 2.84%, n = 6）, respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.
基金sustained by the National Natural Science Foundation of China(No.32001765)the Open Project Program of the State Key Laboratory of Food Nutrition and Safety,China(SKLFNS-KF-202016).
文摘Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 days.The appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold storage.Meanwhile,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ed.Furthermore,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning process.It not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con trolled.Additionally,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and catechol.Conelusion:Hawthorn leaf extract significantly alleviated the browning of fresh-cut potato.It could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.
文摘目的分析山楂叶研究现状和研究热点,为其进一步研究提供参考。方法检索中国知识资源总库(CNKI)、中国学术期刊数据库(万方数据)、中文科技期刊数据库(VIP)、Web of Science(WOS)1980年4月30日-2024年10月31日收录的山楂叶研究文献,采用NoteExpress4.0.0.9855软件进行文献管理和筛选,利用CiteSpace6.2.R6软件对作者、机构、国家和关键词进行可视化分析。结果共纳入中文文献793篇、英文文献122篇,中文文献发文趋势先上升后下降,英文文献发文趋势相对平稳。作者间有一定合作,且形成了团队间、机构间、跨国交流合作,但合作不够密切,中文发文最多的作者为潘海峰,英文发文最多的作者为Li,Yanrong(李艳荣);发文最多的机构为承德医学院。关键词分析显示,化学成分研究以黄酮、多酚、牧荆素、原花青素为主,药理作用研究以糖尿病、高脂血症、抗氧化作用、抗炎为主。结论山楂叶研究仍处于发展上升阶段,山楂叶配方颗粒深度研发可能为今后研究趋势。
