Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria asso...Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria associated with three halophytes in an arid land,Northwest China.The bacterial strains were isolated from the roots,shoots,rhizosphere,and bulk soil of three halophytes,i.e.,Salicornia europaea L.,Kalidium foliatum(Pall.)Moq.,and Suaeda aralocaspica(Bunge)Freitag&Schütze,collected from the saline soils near to the Wujiaqu City,Xinjiang,Northwest China.A total of 567 strains were isolated and identified from these three halophytes belonging to 4 phyla,6 classes,25 orders,36 families,and 66 genera,including 147 potential novel species.A total of 213 strains exhibited one or more plant growthpromoting properties,while 20 strains demonstrated multiple in vitro plant growth-promoting activities,including phosphate solubilization,nitrogen fixation,siderophore production,and production of hydrolytic enzymes such as protease and cellulase.Our findings showed that halophytes in the arid land harbor diverse bacteria with the potential to enhance plant growth and adaptability under challenging environmental conditions.展开更多
Halophytes are valuable salt-, alkali- and drought-resistant germplasm resources. However, the char- acteristics of mineral elements in halophytes have not been investigated as intensively as those in crops. This stud...Halophytes are valuable salt-, alkali- and drought-resistant germplasm resources. However, the char- acteristics of mineral elements in halophytes have not been investigated as intensively as those in crops. This study attempted to investigate the characteristics of mineral elements for annual halophytes during their growth period to reveal their possible physiological mechanisms of salt resistance. By using three native annual halophytes （Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica） distributed in the desert in Northern Xinjiang of China, the dynamic changes in the mineral element contents of annual halophytes were analyzed through field sampling and laboratory analyses. The results demonstrated that the annual halophytes were able to absorb water and mineral nutrients selectively. In the interaction between the annual halophytes and saline soil, the adaptability of the annual halophytes was manifested as the accumulation of S, Na and CI during the growth period and maintenance of water and salt balance in the plant, thus ensuring their selective absorption of N, P, K, Ca, Mg and other mineral nutrients according to their growth demand. By utilizing this property, halophyte planting and mowing （before the wilting and death periods） could bioremediate heavy saline-alkali soil.展开更多
The effects of maternal salinity and light incubation on the salinity tolerance of the facultative halophyte Anabasis setifera during their germination stages were assessed. Seeds were collected from non-saline habita...The effects of maternal salinity and light incubation on the salinity tolerance of the facultative halophyte Anabasis setifera during their germination stages were assessed. Seeds were collected from non-saline habitats in Egypt and saline habitats in the United Arab Emirates （UAE）. The seeds of the two populations were germinated in 0, 100, 200, 400, 600 and 800 mM NaCI, and incubated at 25℃/15℃ in both 12-h light and 12-h darkness regimes and continuous darkness. Significantly more seeds germinated in the Egyptian population than in the UAE population. Salinity tolerance was significantly greater with the Egyptian population than with the UAE population, especially under the conditions of higher salinities. The difference in salinity tolerance between the seeds of two populations was attributed to their seed mass. In addition, germination was significantly faster for the Egyptian population than for the UAE population. Most of the saline treated seeds were able to recover their germination when transferred to distilled water, but this depended on their maternal salinity and light incubation. Recovery from higher salinities was significantly better for the seeds under darkness than for those under light in the UAE population, but the reverse was true for the seeds in the Egyptian population. The higher salinity tolerance for the A. setifera seeds from the non-saline Egyptian population and the lower salinity tolerance for the seeds from the saline UAE population cannot explain their natural distribution. Further studies about other possible roles, such as levels of different promoting and inhibiting phytohormones, are needed to understand the importance of salinity as an environmentally induced maternal effect.展开更多
For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta （YRD）, Shandong Province. The results...For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta （YRD）, Shandong Province. The results showed that the soil mesofauna tended to gather on soil surface in most samples at most times, but the vertical migrating greatly varied in different seasons or environment conditions. Acari was the dominant group. The index of diversity of the soil fauna was correlated with the index of evenness. The Acari＇s number of individuals infected other species and numbers. Dominant group-Aeari made greater contribution to the result of cluster analysis, and there were significant differences between communities in different habitats by cluster analysis with both Bray-Curtis and Jaccard similarity coefficient.展开更多
Endophytic bacteria of halophytic plants play essential roles in salt stress tolerance.Therefore,an understanding of the true nature of plant-microbe interactions under extreme conditions is essential.The current stud...Endophytic bacteria of halophytic plants play essential roles in salt stress tolerance.Therefore,an understanding of the true nature of plant-microbe interactions under extreme conditions is essential.The current study aimed to identify cultivable endophytic bacteria associated with the roots and shoots of Seidlitzia rosmarinus Ehrenb.ex Boiss.grown in the salt-affected soil in Uzbekistan and to evaluate their plant beneficial traits related to plant growth stimulation and stress tolerance.Bacteria were isolated from the roots and the shoots of S.rosmarinus using culture-dependent techniques and identified by the 16S rRNA gene.RFLP(Restriction Fragment Length Polymorphism)analysis was conducted to eliminate similar isolates.Results showed that the isolates from the roots of S.rosmarinus belonged to the genera Rothia,Kocuria,Pseudomonas,Staphylococcus,Paenibacillus and Brevibacterium.The bacterial isolates from the shoots of S.rosmarinus belonged to the genera Staphylococcus,Rothia,Stenotrophomonas,Brevibacterium,Halomonas,Planococcus,Planomicrobium and Pseudomonas,which differed from those of the roots.Notably,Staphylococcus,Rothia and Brevibacterium were detected in both roots and shoots,indicating possible migration of some species from roots to shoots.The root-associated bacteria showed higher levels of IAA(indole-3-acetic acid)synthesis compared with those isolated from the shoots,as well as the higher production of ACC(1-aminocyclopropane-1-carboxylate)deaminase.Our findings suggest that halophytic plants are valuable sources for the selection of microbes with a potential to improve plant fitness under saline soils.展开更多
Endophytic bacteria from halophytes have a wide range of application prospects in various fields,such as plant growth-promoting,biocontrol activity and stress resistance.The current study aimed to identify cultivable ...Endophytic bacteria from halophytes have a wide range of application prospects in various fields,such as plant growth-promoting,biocontrol activity and stress resistance.The current study aimed to identify cultivable endophytic bacteria associated with halophytes grown in the salt-affected soil in Xinjiang Uygur Autonomous Region,China and to evaluate their plant beneficial traits and enzyme-producing activity.Endophytic bacteria were isolated from Reaumuria soongorica(PalL Maxim.),Artemisia carvifolia(Buch.-Ham.ex Roxb.Hort.Beng.),Peganum harmala L.and Suaeda dendroides(C.A.Mey.Moq.)by using the cultural-dependent method.Then we classified these bacteria based on the difference between their sequences of 16S rRNA(16S ribosomal RNA)gene.Results showed that the isolated bacteria from R.soongorica belonged to the genera Brucella,Bacillus and Variovorax.The bacteria from A.carvifolia belonged to the genera Micromonospora and Brucella.The bacteria from P.harmala belonged to the genera Paramesorhizobium,Bacillus and Peribacillus.The bacteria from S.dendroides belonged to the genus Bacillus.Notably,the genus Bacillus was detected in the three above plants,indicating that Bacillus is a common taxon of endophytic bacteria in halophytes.And,our results found that about 37.50%of the tested strains showed strong protease-producing activity,6.25%of the tested strains showed strong cellulase-producing activity and 12.50%of the tested strains showed moderate lipase-producing activity.Besides,all isolated strains were positive for IAA(3-Indoleacetic acid)production,31.25%of isolated strains exhibited a moderate phosphate solubilization activity and 50.00%of isolated strains exhibited a weak siderophore production activity.Our findings suggest that halophytes are valuable resources for identifying microbes with the ability to increase host plant growth and health in salt-affected soils.展开更多
Objective:To evaluate the in vitro anti-Trypanosoma cruzi（T.cruzi） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the ...Objective:To evaluate the in vitro anti-Trypanosoma cruzi（T.cruzi） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the most active samples.Methods:Acetone,dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of T.cruzi.The most active extract was fractionated by preparative HPLC-DAD,affording 11 fractions.The most selective fraction was fully characterized by 1H-NMR.Results:From 94 samples tested,one was active,namely the root dichloromethane extract of Juncus acutus(IC50 < 20 μg/mL).This extract was fractionated by HPLC,affording 11 fractions,one of them containing only a pure compound（juncunol）,and tested for anti-parasitic activity.Fraction 8(IC50 = 4.1 μg/mL) was the most active,and was further characterized by 1H-NMR.The major compounds were phenanthrenes,9,10-dihydrophenanthrenes and benzocoumarins.Conclusion:Our results suggest that the compounds identified in fraction 8 are likely responsible for the observed anti parasitic activity.Further research is in progress aiming to isolate and identify the specific active molecules.To the best of our knowledge,this is the first report on the in vitro anti T.cruzi activity of halophyte species.展开更多
The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity.Since phytochelatins can effectively chelate heavy metals,it was hypothesized that S.salsa possessed a phyt...The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity.Since phytochelatins can effectively chelate heavy metals,it was hypothesized that S.salsa possessed a phytochelatin synthase(PCS) gene.In the present study,the cDNA of PCS was obtained from S.salsa(designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends(RACE).A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides,encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain.A similarity analysis suggested that SsPCS shared up to a 58.6%identity with other PCS proteins and clustered with PCS proteins from eudicots.There was a new kind of metal ion sensor motif in its C-terminal domain.The SsPCS transcript was more highly expressed in elongated and fibered roots and stems(P<0.05) than in leaves.Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS(P<0.05).To the best of our knowledge,SsPCS is the second PCS gene cloned from a halophyte,and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila.This study provided a new view of halophyte PCS genes in heavy metal tolerance.展开更多
Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby...Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby preserves genetic and species diversity for future use.We evaluated ex situ storage potential of eight halophytic species from deserts in the United Arab Emirates(UAE)by studying seed germination.Specifically,we examined the germinability of freshly collected seeds and seeds stored for three years in a seed bank.We also examined the effect of light conditions on fresh and stored seed germination.Fresh seeds of seven of the eight species tested had a higher germination rates under 12/12 h light/dark fluctuations than did those exposed to total darkness.Storage reduced light sensitivity in Halocnemum strobilaceum,Suaeda aegyptiaca,Salsola drummondii and Salsola imbricata,but increased the requirement for light in Arthrocnemum macrostachyum.In Anabasis setifera,storage decreased germination percentage when there was a 12-hour light/dark fluctuation,but increased germination rate when exposed to the dark treatment.Storage significantly reduced germination in both the light/dark and dark treatments in Suaeda vermiculata and S.aegyptiaca.Germination speed also responded differently to storage;whereas Timson's index significantly increased in A.macrostachyum and H.strobilaceum,it significantly decreased for S.drummondii,S.aegyptiaca and S.vermiculata.Germination of these species at a range of temperatures requires further testing;additionally,we strongly suggest that these laboratory findings be complemented by field studies.展开更多
About 23%of the surface area and 44%of the volume of all the lakes are occupied by saline lakes in the world.Importantly,agricultural diversion,illegal encroachment,pollution,and invasive species could cause these lak...About 23%of the surface area and 44%of the volume of all the lakes are occupied by saline lakes in the world.Importantly,agricultural diversion,illegal encroachment,pollution,and invasive species could cause these lakes to dry up completely or partially by 2025.Illegal saltpan encroachment is causing Sambhar,India’s largest saline lake,to shrink by 4.23%every decade.This study aims to characterize the soil parameters where halophytes are growing.A literature survey was conducted for halophytes and soil characteristics.The study area was divided into four zones for stratified random sampling.Soil sampling was conducted in February 2021.The soil indicators for halophyte selected were pH,electrical conductivity,moisture,salinity,organic carbon,and organic matter.The obtained results were interpolated in the geospatial platform for soil characteristic mapping.It is found that no research is conducted on halophytes of the lake.Studies on soil are also inconsistent and only six common parameters could be identified.Results show that the pH ranged 9.37-7.66,electrical conductivity was 16.1-0.38,moisture 23.37%-1.2%,organic carbon 3.29%-0.15%,organic matter 5.6%-0.2%,and salinity 8.86%-0.72%.Though these results show improved condition as compared to last few years,in long term,the lake is desiccating.During the UN Decade of Ecosystem Restoration(2021-2030),if these causes are not addressed,the ecosystem may completely dry up.展开更多
In the pot experiment,seven varieties of halophytes were grown in saline-alkali soil to investigate the responses of microflora and soil enzymes in the rhizosphere.The results showed that compared to the control,the p...In the pot experiment,seven varieties of halophytes were grown in saline-alkali soil to investigate the responses of microflora and soil enzymes in the rhizosphere.The results showed that compared to the control,the population of bacterial colony(84.8%-95.6%),actinomycetes colony(12.0%-14.5%)and fungi colony(0.5%-1.1%)increased significantly(P<0.05).The population of ammonia bacteria,aerobic cellulose decomposition bacteria in the soil of Vicia sativa L.(201.99%and 395.49%),Medicago sativa(152.43%and 319.90%)and Sesbania cannabina(Retz.)Pori(193.14%and 396.08%)were higher significantly than that of Panicum virgatum L.(49%and 60%),Sorghum bicolor(L.)Moench(99%and 210%),Amaranthus hypochondriacus L.(75%and 36%)and Aneurotepidimu chinense(75%and 77%)(P<0.05).However,Sorghum bicolor(L.)Moench was evidently higher than Panicum virgatum L.,Amaranthus hypochondriacus L.and Aneurotepidimu chinense(P<0.05)in the soil.The population of ammonia bacteria and aerobic cellulose-decomposing bacteria was significantly correlated with the five enzymes(P<0.05),which could improve the microenvironment in saline-alkali soil to accelerate the element cycling and promote the sustainable development of agriculture through cultivating Medicago sativa,Vicia sativa L.,Sesbania cannabina(Retz.)Pori and Sorghum bicolor(L.)Moench.展开更多
In this paper, we constructed the halophyte Mesocosm experimental group which was used as a substrate material that consisted of tidal flat and dredged sediment. Depending on whether the ingredients of vegetation and ...In this paper, we constructed the halophyte Mesocosm experimental group which was used as a substrate material that consisted of tidal flat and dredged sediment. Depending on whether the ingredients of vegetation and substrate material of Mesocosm, we constructed Mesocosm A (tidal flat sediment + Salicornia herbecea), Mesocosm B (only dredged sediment), Mesocosm C (dredged sediment + Salicornia herbecea). Monitoring was carried out of seawater quality factors (Chemical Oxygen Demand (COD), Total Nitrogen (T-N), Total Phosphorus (T-P), temperature, salinity), sediment factors (Chemical Oxygen Demand (COD), Total (T-N), Total Phosphorus (T-P) and growth of Salricornia herbecea) in each Mesocosm. Habitat Stability Index of vegetation was calculating by using the monitoring results. HSI of Mesocosm C was calculated from 0.87 to 0.95 as compared to the relatively high HSI Mesocosm A, it was evaluated to be able to be used in the restoration of the coastal salt marsh with dredged sediment.展开更多
To know the mode of distribution of halophytes species in the western region of Algeria, it was considered useful to undertake a study based on the achievement of identified linear. Two areas were considered, Northern...To know the mode of distribution of halophytes species in the western region of Algeria, it was considered useful to undertake a study based on the achievement of identified linear. Two areas were considered, Northern Tlemcen (Beni Saf and Rachgoun) and Northwest Oran (Sabkha). The trend of climate aridity which is more pronounced in the study area may have influences on floristic halophytes. The study of the floristic composition showed the importance of indicator halophitisation species such as Atriplex halimus, Tamarix gallica, Salicornia fruticosa, Arthrocnemum glaucum and was accompanied by various therophytes such as Sinapis arvensis, Erodium moschatum, Malva sylvestris and Plantago lagopus. ANOVA (analysis of variance) has highlighted the factors that influence the spatial distribution of some halophytes species at five stations studied;some are dispersed aggregates (Tamarix gallica, Suaeda fruticosa) with a coefficient of variation (Cv) greater than 10%;others are regularly dispersed (Atriplex halimus) with a coefficient of variation (Cv) less than 10%.展开更多
Coastal wetlands face dual pressures from high salinity and heavy metal pollution,presenting significant ecological challenges.Halophytes like Sesuvium portulacastrum possess unique physiological mechanisms to mitigat...Coastal wetlands face dual pressures from high salinity and heavy metal pollution,presenting significant ecological challenges.Halophytes like Sesuvium portulacastrum possess unique physiological mechanisms to mitigate metal toxicity.This study investigates how silicon (Si) availability influences the accumulation of copper (Cu) and cadmium (Cd) in S.portulacastrum.Our results show that Si supplementation at environmentally relevant levels significantly increases Cu and Cd concentrations in the roots,while simultaneously reducing the root-to-shoot translocation of these metals.In situ non-invasive micro-testing revealed decreased metal efflux from the xylem,indicating an enhanced retention of metals in the roots.Furthermore,analyses using X-ray photoelectron spectroscopy and atomic force microscopy demonstrated a higher density of oxygen-containing functional groups and SiO-on the extracellular matrix of Si-enriched roots.This structural transformation resulted in a significant reduction in root surface potential,facilitating greater metal ion attraction and uptake.The findings from this study provide critical insights into the mechanisms by which Si availability regulates metal accumulation in halophytes,suggesting potential strategies for mitigating metal pollution in coastal wetland ecosystems.展开更多
Glycophyte biomass-derived biochars are currently concerned in most studies.However,little attention is given to the char-acteristics of halophyte-derived biochars.In this study,five typical halophytes of euhalophytes...Glycophyte biomass-derived biochars are currently concerned in most studies.However,little attention is given to the char-acteristics of halophyte-derived biochars.In this study,five typical halophytes of euhalophytes(Suaeda altissima,Suaeda salsa,and Kalidium foliatum),recretohalophytes(Phragmites australis),and pseudohalophytes(Tamarix chinensis)which are widely distributed in the arid and semiarid regions of northwestern China were selected for producing biochars with a slow pyrolysis process at 500℃for 1 h.The harvested biochars were characterized in elemental content,pores,surface area,and surface charges,and then their potential value as a soil conditioner was evaluated.The results showed that the halophyte-derived biochars had variable ash and Na+contents,ranging from 7.26 to 23.64%and 1.06 to 33.93 g kg^(−1)respectively.The EC value of the biochars ranged from 1.76 to 23.45 mS cm^(−1).The biochar derived from Suaeda altissima had a very low specific surface area(SSA),3.50 m^(2)g^(−1),while that derived from Phragmites australis(BPA)had a very high SSA,344.02 m^(2)g^(−1).All the biochars carried both positive and negative charges.Kalidium foliatum biochar(BKF)possessed more nega-tive charges,while Suaeda altissima biochar(BSA)contained more positive charges.In general,the halophyte biochars had a higher ash content and lower point of zero net charge(PZNC)value,compared with the biochars derived from glycophytes,which would imply their higher potential value as an acidic soil conditioner.展开更多
The effects of NaCl stress on the activity of anti-oxidant enzymes （superoxide dismutase, catalase （CAT）, peroxidase （POD）, ascorbate peroxidase （APX）, monodehydroascorbate reductase, dehydroascorbate reductase...The effects of NaCl stress on the activity of anti-oxidant enzymes （superoxide dismutase, catalase （CAT）, peroxidase （POD）, ascorbate peroxidase （APX）, monodehydroascorbate reductase, dehydroascorbate reductase （DHAR）, and glutathione reductase （GR））, anti-oxidant molecules （ascorbate and glutathione）, and parameters of oxidative stress （malondialdehyde （MDA）, electrolyte leakage, and H2O2 concentrations） were investigated in Cakile maritima, a halophyte frequent along the Tunisian seashore. Seedlings were grown in the presence of salt （100, 200, and 400 mmol/L NaCl）. Plants were harvested periodically over 20 days. Growth was maximal in the presence of 0-100 mmol/L NaCl. At 400 mmol/L NaCl, growth decreased significantly. The salt tolerance of C. maritima, at moderate salinities, was associated with the lowest values of the parameters indicative of oxidative stress, namely the highest activities of POD, CAT, APX, DHAR, and GR and high tissue content of ascorbate and glutathione. However, prolonged exposure to high salinity resulted in a decrease in anti-oxidant activities and high MDA content, electrolyte leakage, and H2O2 concentrations. These results suggest that anti-oxidant systems participate in the tolerance of C. maritima to moderate salinities.展开更多
Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A...Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.展开更多
Suaeda fruticosa is an edible medicinal species belonging to the Amaranthaceae (Chenopodiaceae) family widely distributed in the Mediterranean regions, especially in Tunisia. This species shoots change their colour fr...Suaeda fruticosa is an edible medicinal species belonging to the Amaranthaceae (Chenopodiaceae) family widely distributed in the Mediterranean regions, especially in Tunisia. This species shoots change their colour from green to red-violet along the different seasons, being an interesting matrix to study for its chemical composition. Therefore, in a ground-breaking study, the hydromethanolic extracts of green and red-violet Tunisian S. fruticosa shoots were profiled for their individual phenolic compounds and betalains by High Performance Liquid Chromatography coupled with a Diode-Array Detector and Mass Spectrometry by Electrospray Ionization (HPLC-DAD/ESI-MS), and the in vitro antioxidant, antibacterial, and hepatotoxic properties were also assessed. The obtained results revealed that the hydromethanolic extracts contain interesting levels of phenolic compounds especially O-glycosylated and acylated flavonoids, while betacyanins were the main betalain compounds found. As expected, red-violet shoots were richer in phenolic compounds (45.5 ± 0.2 mg/g extract) and betalains (33.3 ± 0.2 mg/g extract) than greener shoots. Flavonoid/betalains rich extracts presented lower EC50 values for ABTS+, DPPH, and TBARS;as also lower MIC values against Gram-positive bacteria. This study showed that the wild halophyte growing in a hard environment, were drought and salinity are dominant, is an important source of flavonoids and betanin, important molecules that can find application in different industries, given the constant demands for natural bioactives and colouring compounds. Overall, these natural compounds may be valorised and further investigated to validate their potential as functional ingredients.展开更多
Soil salinization may affect biodiversity and species composition,leading to changes in the plant community structure.However,few studies have explored the spatial pattern of soil salinization and its effects on shrub...Soil salinization may affect biodiversity and species composition,leading to changes in the plant community structure.However,few studies have explored the spatial pattern of soil salinization and its effects on shrub community structure at the ecosystem scale.Therefore,we conducted a transect sampling of desert shrublands in Northwest China during the growing season(June–September)in 2021.Soil salinization(both the degree and type),shrub community structure(e.g.,shrub density and height),and biodiversity parameters(e.g.,Simpson diversity,Margalf abundance,Shannon-Wiener diversity,and Pielou evenness indices)were used to assess the effects of soil salinization on shrub community structure.The results showed that the primary degree of soil salinization in the study area was light salinization,with the area proportion of 69.8%.Whereas the main type of soil salinization was characterized as sulfate saline soil,also accounting for 69.8%of the total area.Notably,there was a significant reduction in the degree of soil salinization and a shift in the type of soil salinization from chloride saline soil to sulfate saline soil,with an increase in longitude.Regional mean annual precipitation(MAP),mean annual evapotranspiration(MAE),elevation,and slope significantly contributed to soil salinization and its geochemical differentiation.As soil salinization intensified,shrub community structure displayed increased diversity and evenness,as indicated by the increases in the Simpson diversity,Shannon-Wiener diversity,and Pielou evenness indices.Moreover,the succulent stems and leaves of Chenopodiaceae and Tamaricaceae exhibited clear advantages under these conditions.Furthermore,regional climate and topography,such as MAP,MAE,and elevation,had greater effects on the distribution of shrub plants than soil salinization.These results provide a reference for the origin and pattern of soil salinization in drylands and their effects on the community structure of halophyte shrub species.展开更多
基金funded by the Key Research and Development Project of Xinjiang Uygur Autonomous Region (2024B02015-3)the Regional Coordinated Innovation Project (Shanghai Cooperation Organization Science and Technology Partnership Program) of Xinjiang Uygur Autonomous Region (2025E01024)
文摘Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria associated with three halophytes in an arid land,Northwest China.The bacterial strains were isolated from the roots,shoots,rhizosphere,and bulk soil of three halophytes,i.e.,Salicornia europaea L.,Kalidium foliatum(Pall.)Moq.,and Suaeda aralocaspica(Bunge)Freitag&Schütze,collected from the saline soils near to the Wujiaqu City,Xinjiang,Northwest China.A total of 567 strains were isolated and identified from these three halophytes belonging to 4 phyla,6 classes,25 orders,36 families,and 66 genera,including 147 potential novel species.A total of 213 strains exhibited one or more plant growthpromoting properties,while 20 strains demonstrated multiple in vitro plant growth-promoting activities,including phosphate solubilization,nitrogen fixation,siderophore production,and production of hydrolytic enzymes such as protease and cellulase.Our findings showed that halophytes in the arid land harbor diverse bacteria with the potential to enhance plant growth and adaptability under challenging environmental conditions.
基金financially supported by the Scientific and Technological Project of Xinjiang Uygur Autonomous Region(201130106-2)the Innovation and Sustainable Development Research on Forest Carbon Sink in Karamay
文摘Halophytes are valuable salt-, alkali- and drought-resistant germplasm resources. However, the char- acteristics of mineral elements in halophytes have not been investigated as intensively as those in crops. This study attempted to investigate the characteristics of mineral elements for annual halophytes during their growth period to reveal their possible physiological mechanisms of salt resistance. By using three native annual halophytes （Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica） distributed in the desert in Northern Xinjiang of China, the dynamic changes in the mineral element contents of annual halophytes were analyzed through field sampling and laboratory analyses. The results demonstrated that the annual halophytes were able to absorb water and mineral nutrients selectively. In the interaction between the annual halophytes and saline soil, the adaptability of the annual halophytes was manifested as the accumulation of S, Na and CI during the growth period and maintenance of water and salt balance in the plant, thus ensuring their selective absorption of N, P, K, Ca, Mg and other mineral nutrients according to their growth demand. By utilizing this property, halophyte planting and mowing （before the wilting and death periods） could bioremediate heavy saline-alkali soil.
基金supported by the Qatar National Research Fund(5-260-1-053)
文摘The effects of maternal salinity and light incubation on the salinity tolerance of the facultative halophyte Anabasis setifera during their germination stages were assessed. Seeds were collected from non-saline habitats in Egypt and saline habitats in the United Arab Emirates （UAE）. The seeds of the two populations were germinated in 0, 100, 200, 400, 600 and 800 mM NaCI, and incubated at 25℃/15℃ in both 12-h light and 12-h darkness regimes and continuous darkness. Significantly more seeds germinated in the Egyptian population than in the UAE population. Salinity tolerance was significantly greater with the Egyptian population than with the UAE population, especially under the conditions of higher salinities. The difference in salinity tolerance between the seeds of two populations was attributed to their seed mass. In addition, germination was significantly faster for the Egyptian population than for the UAE population. Most of the saline treated seeds were able to recover their germination when transferred to distilled water, but this depended on their maternal salinity and light incubation. Recovery from higher salinities was significantly better for the seeds under darkness than for those under light in the UAE population, but the reverse was true for the seeds in the Egyptian population. The higher salinity tolerance for the A. setifera seeds from the non-saline Egyptian population and the lower salinity tolerance for the seeds from the saline UAE population cannot explain their natural distribution. Further studies about other possible roles, such as levels of different promoting and inhibiting phytohormones, are needed to understand the importance of salinity as an environmentally induced maternal effect.
基金Supported by the Doctoral Fund of Northeast Agricultural University(2009RC41)Postdoctoral Grants of Heilongjiang Province(LBH-Z10265)
文摘For the first time, we used Tullgren method made a study on vertical migrating and cluster analysis of the soil mesofauna in Dongying Halophytes Garden in the Yellow River Delta （YRD）, Shandong Province. The results showed that the soil mesofauna tended to gather on soil surface in most samples at most times, but the vertical migrating greatly varied in different seasons or environment conditions. Acari was the dominant group. The index of diversity of the soil fauna was correlated with the index of evenness. The Acari＇s number of individuals infected other species and numbers. Dominant group-Aeari made greater contribution to the result of cluster analysis, and there were significant differences between communities in different habitats by cluster analysis with both Bray-Curtis and Jaccard similarity coefficient.
基金the Eurasia Program of the Norwegian Centre for Cooperation in Education(CPEA-LT-2016/10095)the German Academic Exchange Service(DAAD)the President's International Fellowship Initiative of the Chinese Academy of Sciences(2018VBA002S).
文摘Endophytic bacteria of halophytic plants play essential roles in salt stress tolerance.Therefore,an understanding of the true nature of plant-microbe interactions under extreme conditions is essential.The current study aimed to identify cultivable endophytic bacteria associated with the roots and shoots of Seidlitzia rosmarinus Ehrenb.ex Boiss.grown in the salt-affected soil in Uzbekistan and to evaluate their plant beneficial traits related to plant growth stimulation and stress tolerance.Bacteria were isolated from the roots and the shoots of S.rosmarinus using culture-dependent techniques and identified by the 16S rRNA gene.RFLP(Restriction Fragment Length Polymorphism)analysis was conducted to eliminate similar isolates.Results showed that the isolates from the roots of S.rosmarinus belonged to the genera Rothia,Kocuria,Pseudomonas,Staphylococcus,Paenibacillus and Brevibacterium.The bacterial isolates from the shoots of S.rosmarinus belonged to the genera Staphylococcus,Rothia,Stenotrophomonas,Brevibacterium,Halomonas,Planococcus,Planomicrobium and Pseudomonas,which differed from those of the roots.Notably,Staphylococcus,Rothia and Brevibacterium were detected in both roots and shoots,indicating possible migration of some species from roots to shoots.The root-associated bacteria showed higher levels of IAA(indole-3-acetic acid)synthesis compared with those isolated from the shoots,as well as the higher production of ACC(1-aminocyclopropane-1-carboxylate)deaminase.Our findings suggest that halophytic plants are valuable sources for the selection of microbes with a potential to improve plant fitness under saline soils.
基金This research was supported by the Xinjiang Uygur Autonomous Region Regional Coordinated Innovation Project(Shanghai Cooperation Organization Science and Technology Partnership Program)(2020E01047)the National Natural Science Foundation of China(U1703106,32061143043,91751206)The authors acknowledge the anonymous reviewers for their constructive comments on the manuscript.
文摘Endophytic bacteria from halophytes have a wide range of application prospects in various fields,such as plant growth-promoting,biocontrol activity and stress resistance.The current study aimed to identify cultivable endophytic bacteria associated with halophytes grown in the salt-affected soil in Xinjiang Uygur Autonomous Region,China and to evaluate their plant beneficial traits and enzyme-producing activity.Endophytic bacteria were isolated from Reaumuria soongorica(PalL Maxim.),Artemisia carvifolia(Buch.-Ham.ex Roxb.Hort.Beng.),Peganum harmala L.and Suaeda dendroides(C.A.Mey.Moq.)by using the cultural-dependent method.Then we classified these bacteria based on the difference between their sequences of 16S rRNA(16S ribosomal RNA)gene.Results showed that the isolated bacteria from R.soongorica belonged to the genera Brucella,Bacillus and Variovorax.The bacteria from A.carvifolia belonged to the genera Micromonospora and Brucella.The bacteria from P.harmala belonged to the genera Paramesorhizobium,Bacillus and Peribacillus.The bacteria from S.dendroides belonged to the genus Bacillus.Notably,the genus Bacillus was detected in the three above plants,indicating that Bacillus is a common taxon of endophytic bacteria in halophytes.And,our results found that about 37.50%of the tested strains showed strong protease-producing activity,6.25%of the tested strains showed strong cellulase-producing activity and 12.50%of the tested strains showed moderate lipase-producing activity.Besides,all isolated strains were positive for IAA(3-Indoleacetic acid)production,31.25%of isolated strains exhibited a moderate phosphate solubilization activity and 50.00%of isolated strains exhibited a weak siderophore production activity.Our findings suggest that halophytes are valuable resources for identifying microbes with the ability to increase host plant growth and health in salt-affected soils.
基金supported by the Xtreme Bio(PTDC/MAR-EST/4346/2012)Ma Na Cruzi projects(bilateral project,FCT/CAPES 2358,2014/2015)+3 种基金funded by FCT–Foundation for Science and Technology and Portuguese National Budgetit also received national funds through FCT project CCMAR/Multi/04326/2013 and P3DPrograma de Descoberta e Desenvolvimento de Drogas(PROEP/CNPq/FIOCRUZ 401988/2012-0)supported by FCT Investigator Programme(IF/00049/2012)Policarpo Sales by Programa Brasil Sem Miséria/Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior(CAPES)/FIOCRUZ
文摘Objective:To evaluate the in vitro anti-Trypanosoma cruzi（T.cruzi） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the most active samples.Methods:Acetone,dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of T.cruzi.The most active extract was fractionated by preparative HPLC-DAD,affording 11 fractions.The most selective fraction was fully characterized by 1H-NMR.Results:From 94 samples tested,one was active,namely the root dichloromethane extract of Juncus acutus(IC50 < 20 μg/mL).This extract was fractionated by HPLC,affording 11 fractions,one of them containing only a pure compound（juncunol）,and tested for anti-parasitic activity.Fraction 8(IC50 = 4.1 μg/mL) was the most active,and was further characterized by 1H-NMR.The major compounds were phenanthrenes,9,10-dihydrophenanthrenes and benzocoumarins.Conclusion:Our results suggest that the compounds identified in fraction 8 are likely responsible for the observed anti parasitic activity.Further research is in progress aiming to isolate and identify the specific active molecules.To the best of our knowledge,this is the first report on the in vitro anti T.cruzi activity of halophyte species.
基金Supported by the 100 Talents Program of the Chinese Academy of Sciencesthe Key Technology R&D Program of Shandong Province(No.2012GGA06032)
文摘The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity.Since phytochelatins can effectively chelate heavy metals,it was hypothesized that S.salsa possessed a phytochelatin synthase(PCS) gene.In the present study,the cDNA of PCS was obtained from S.salsa(designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends(RACE).A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides,encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain.A similarity analysis suggested that SsPCS shared up to a 58.6%identity with other PCS proteins and clustered with PCS proteins from eudicots.There was a new kind of metal ion sensor motif in its C-terminal domain.The SsPCS transcript was more highly expressed in elongated and fibered roots and stems(P<0.05) than in leaves.Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS(P<0.05).To the best of our knowledge,SsPCS is the second PCS gene cloned from a halophyte,and it might contain a different metal sensing capability than the first PCS from Thellungiella halophila.This study provided a new view of halophyte PCS genes in heavy metal tolerance.
文摘Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby preserves genetic and species diversity for future use.We evaluated ex situ storage potential of eight halophytic species from deserts in the United Arab Emirates(UAE)by studying seed germination.Specifically,we examined the germinability of freshly collected seeds and seeds stored for three years in a seed bank.We also examined the effect of light conditions on fresh and stored seed germination.Fresh seeds of seven of the eight species tested had a higher germination rates under 12/12 h light/dark fluctuations than did those exposed to total darkness.Storage reduced light sensitivity in Halocnemum strobilaceum,Suaeda aegyptiaca,Salsola drummondii and Salsola imbricata,but increased the requirement for light in Arthrocnemum macrostachyum.In Anabasis setifera,storage decreased germination percentage when there was a 12-hour light/dark fluctuation,but increased germination rate when exposed to the dark treatment.Storage significantly reduced germination in both the light/dark and dark treatments in Suaeda vermiculata and S.aegyptiaca.Germination speed also responded differently to storage;whereas Timson's index significantly increased in A.macrostachyum and H.strobilaceum,it significantly decreased for S.drummondii,S.aegyptiaca and S.vermiculata.Germination of these species at a range of temperatures requires further testing;additionally,we strongly suggest that these laboratory findings be complemented by field studies.
文摘About 23%of the surface area and 44%of the volume of all the lakes are occupied by saline lakes in the world.Importantly,agricultural diversion,illegal encroachment,pollution,and invasive species could cause these lakes to dry up completely or partially by 2025.Illegal saltpan encroachment is causing Sambhar,India’s largest saline lake,to shrink by 4.23%every decade.This study aims to characterize the soil parameters where halophytes are growing.A literature survey was conducted for halophytes and soil characteristics.The study area was divided into four zones for stratified random sampling.Soil sampling was conducted in February 2021.The soil indicators for halophyte selected were pH,electrical conductivity,moisture,salinity,organic carbon,and organic matter.The obtained results were interpolated in the geospatial platform for soil characteristic mapping.It is found that no research is conducted on halophytes of the lake.Studies on soil are also inconsistent and only six common parameters could be identified.Results show that the pH ranged 9.37-7.66,electrical conductivity was 16.1-0.38,moisture 23.37%-1.2%,organic carbon 3.29%-0.15%,organic matter 5.6%-0.2%,and salinity 8.86%-0.72%.Though these results show improved condition as compared to last few years,in long term,the lake is desiccating.During the UN Decade of Ecosystem Restoration(2021-2030),if these causes are not addressed,the ecosystem may completely dry up.
基金Supported by Funding(Topic CXZ003)of Key Research and Development Plan of Ningxia Hui Autonomous Region(2019BBF02001,2018BBF23008)。
文摘In the pot experiment,seven varieties of halophytes were grown in saline-alkali soil to investigate the responses of microflora and soil enzymes in the rhizosphere.The results showed that compared to the control,the population of bacterial colony(84.8%-95.6%),actinomycetes colony(12.0%-14.5%)and fungi colony(0.5%-1.1%)increased significantly(P<0.05).The population of ammonia bacteria,aerobic cellulose decomposition bacteria in the soil of Vicia sativa L.(201.99%and 395.49%),Medicago sativa(152.43%and 319.90%)and Sesbania cannabina(Retz.)Pori(193.14%and 396.08%)were higher significantly than that of Panicum virgatum L.(49%and 60%),Sorghum bicolor(L.)Moench(99%and 210%),Amaranthus hypochondriacus L.(75%and 36%)and Aneurotepidimu chinense(75%and 77%)(P<0.05).However,Sorghum bicolor(L.)Moench was evidently higher than Panicum virgatum L.,Amaranthus hypochondriacus L.and Aneurotepidimu chinense(P<0.05)in the soil.The population of ammonia bacteria and aerobic cellulose-decomposing bacteria was significantly correlated with the five enzymes(P<0.05),which could improve the microenvironment in saline-alkali soil to accelerate the element cycling and promote the sustainable development of agriculture through cultivating Medicago sativa,Vicia sativa L.,Sesbania cannabina(Retz.)Pori and Sorghum bicolor(L.)Moench.
文摘In this paper, we constructed the halophyte Mesocosm experimental group which was used as a substrate material that consisted of tidal flat and dredged sediment. Depending on whether the ingredients of vegetation and substrate material of Mesocosm, we constructed Mesocosm A (tidal flat sediment + Salicornia herbecea), Mesocosm B (only dredged sediment), Mesocosm C (dredged sediment + Salicornia herbecea). Monitoring was carried out of seawater quality factors (Chemical Oxygen Demand (COD), Total Nitrogen (T-N), Total Phosphorus (T-P), temperature, salinity), sediment factors (Chemical Oxygen Demand (COD), Total (T-N), Total Phosphorus (T-P) and growth of Salricornia herbecea) in each Mesocosm. Habitat Stability Index of vegetation was calculating by using the monitoring results. HSI of Mesocosm C was calculated from 0.87 to 0.95 as compared to the relatively high HSI Mesocosm A, it was evaluated to be able to be used in the restoration of the coastal salt marsh with dredged sediment.
文摘To know the mode of distribution of halophytes species in the western region of Algeria, it was considered useful to undertake a study based on the achievement of identified linear. Two areas were considered, Northern Tlemcen (Beni Saf and Rachgoun) and Northwest Oran (Sabkha). The trend of climate aridity which is more pronounced in the study area may have influences on floristic halophytes. The study of the floristic composition showed the importance of indicator halophitisation species such as Atriplex halimus, Tamarix gallica, Salicornia fruticosa, Arthrocnemum glaucum and was accompanied by various therophytes such as Sinapis arvensis, Erodium moschatum, Malva sylvestris and Plantago lagopus. ANOVA (analysis of variance) has highlighted the factors that influence the spatial distribution of some halophytes species at five stations studied;some are dispersed aggregates (Tamarix gallica, Suaeda fruticosa) with a coefficient of variation (Cv) greater than 10%;others are regularly dispersed (Atriplex halimus) with a coefficient of variation (Cv) less than 10%.
基金supported by the National Natural Science Foundation of China (Nos.42376152 and 42306155)Guangdong Major Project of Basic and Applied Basic Research(No.2023B0303000017)+2 种基金the Special Program of Key Sectors in Guangdong Universities (No.2022ZDZX4040)the Innovation Team Project of Universities in Guangdong Province (No.2023KCXTD028)Guangxi Key Laboratory of Marine Environmental Change and Disaster in Beibu Gulf,Beibu Gulf University (No.2022KF007)。
文摘Coastal wetlands face dual pressures from high salinity and heavy metal pollution,presenting significant ecological challenges.Halophytes like Sesuvium portulacastrum possess unique physiological mechanisms to mitigate metal toxicity.This study investigates how silicon (Si) availability influences the accumulation of copper (Cu) and cadmium (Cd) in S.portulacastrum.Our results show that Si supplementation at environmentally relevant levels significantly increases Cu and Cd concentrations in the roots,while simultaneously reducing the root-to-shoot translocation of these metals.In situ non-invasive micro-testing revealed decreased metal efflux from the xylem,indicating an enhanced retention of metals in the roots.Furthermore,analyses using X-ray photoelectron spectroscopy and atomic force microscopy demonstrated a higher density of oxygen-containing functional groups and SiO-on the extracellular matrix of Si-enriched roots.This structural transformation resulted in a significant reduction in root surface potential,facilitating greater metal ion attraction and uptake.The findings from this study provide critical insights into the mechanisms by which Si availability regulates metal accumulation in halophytes,suggesting potential strategies for mitigating metal pollution in coastal wetland ecosystems.
基金the 13th five-year project of National Key Technology R&D Program(No.2016YFC0501306-02)the National Key Research and Development Program of China(2016YFC0501306).
文摘Glycophyte biomass-derived biochars are currently concerned in most studies.However,little attention is given to the char-acteristics of halophyte-derived biochars.In this study,five typical halophytes of euhalophytes(Suaeda altissima,Suaeda salsa,and Kalidium foliatum),recretohalophytes(Phragmites australis),and pseudohalophytes(Tamarix chinensis)which are widely distributed in the arid and semiarid regions of northwestern China were selected for producing biochars with a slow pyrolysis process at 500℃for 1 h.The harvested biochars were characterized in elemental content,pores,surface area,and surface charges,and then their potential value as a soil conditioner was evaluated.The results showed that the halophyte-derived biochars had variable ash and Na+contents,ranging from 7.26 to 23.64%and 1.06 to 33.93 g kg^(−1)respectively.The EC value of the biochars ranged from 1.76 to 23.45 mS cm^(−1).The biochar derived from Suaeda altissima had a very low specific surface area(SSA),3.50 m^(2)g^(−1),while that derived from Phragmites australis(BPA)had a very high SSA,344.02 m^(2)g^(−1).All the biochars carried both positive and negative charges.Kalidium foliatum biochar(BKF)possessed more nega-tive charges,while Suaeda altissima biochar(BSA)contained more positive charges.In general,the halophyte biochars had a higher ash content and lower point of zero net charge(PZNC)value,compared with the biochars derived from glycophytes,which would imply their higher potential value as an acidic soil conditioner.
文摘The effects of NaCl stress on the activity of anti-oxidant enzymes （superoxide dismutase, catalase （CAT）, peroxidase （POD）, ascorbate peroxidase （APX）, monodehydroascorbate reductase, dehydroascorbate reductase （DHAR）, and glutathione reductase （GR））, anti-oxidant molecules （ascorbate and glutathione）, and parameters of oxidative stress （malondialdehyde （MDA）, electrolyte leakage, and H2O2 concentrations） were investigated in Cakile maritima, a halophyte frequent along the Tunisian seashore. Seedlings were grown in the presence of salt （100, 200, and 400 mmol/L NaCl）. Plants were harvested periodically over 20 days. Growth was maximal in the presence of 0-100 mmol/L NaCl. At 400 mmol/L NaCl, growth decreased significantly. The salt tolerance of C. maritima, at moderate salinities, was associated with the lowest values of the parameters indicative of oxidative stress, namely the highest activities of POD, CAT, APX, DHAR, and GR and high tissue content of ascorbate and glutathione. However, prolonged exposure to high salinity resulted in a decrease in anti-oxidant activities and high MDA content, electrolyte leakage, and H2O2 concentrations. These results suggest that anti-oxidant systems participate in the tolerance of C. maritima to moderate salinities.
基金supported by a grant from the Cooperative Research Projects for Bioenergy Crop Development RDA (RIMS20070201036026)
文摘Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.
基金grateful to The Tunisian Ministry of High Education and research for financial support through national funds to Laboratory of Bioressources Integrative Biology and valorizatingrateful the Foundation for Science and Technology(FCT,Portugal)for financial support through national funds FCT/MCTES to CIMO(UIDB/00690/2020)and SusTEC(LA/P/0007/2021)+1 种基金the F.Mandim PhD grant(SFRH/BD/146614/2019)L.Barros,M.I.Dias,and R.Calhelha thank the national funding by FCT,P.I.,through the institutional and S.A.Heleno to the individual scientific employment program-contract,for their contracts.
文摘Suaeda fruticosa is an edible medicinal species belonging to the Amaranthaceae (Chenopodiaceae) family widely distributed in the Mediterranean regions, especially in Tunisia. This species shoots change their colour from green to red-violet along the different seasons, being an interesting matrix to study for its chemical composition. Therefore, in a ground-breaking study, the hydromethanolic extracts of green and red-violet Tunisian S. fruticosa shoots were profiled for their individual phenolic compounds and betalains by High Performance Liquid Chromatography coupled with a Diode-Array Detector and Mass Spectrometry by Electrospray Ionization (HPLC-DAD/ESI-MS), and the in vitro antioxidant, antibacterial, and hepatotoxic properties were also assessed. The obtained results revealed that the hydromethanolic extracts contain interesting levels of phenolic compounds especially O-glycosylated and acylated flavonoids, while betacyanins were the main betalain compounds found. As expected, red-violet shoots were richer in phenolic compounds (45.5 ± 0.2 mg/g extract) and betalains (33.3 ± 0.2 mg/g extract) than greener shoots. Flavonoid/betalains rich extracts presented lower EC50 values for ABTS+, DPPH, and TBARS;as also lower MIC values against Gram-positive bacteria. This study showed that the wild halophyte growing in a hard environment, were drought and salinity are dominant, is an important source of flavonoids and betanin, important molecules that can find application in different industries, given the constant demands for natural bioactives and colouring compounds. Overall, these natural compounds may be valorised and further investigated to validate their potential as functional ingredients.
基金financially supported by the National Natural Sciences Foundation of China(42330503,42171068)the Third Xinjiang Scientific Expedition Program(2022xjkk0901)the Tianshan Talent Training Program(2023TSYCLJ0048).
文摘Soil salinization may affect biodiversity and species composition,leading to changes in the plant community structure.However,few studies have explored the spatial pattern of soil salinization and its effects on shrub community structure at the ecosystem scale.Therefore,we conducted a transect sampling of desert shrublands in Northwest China during the growing season(June–September)in 2021.Soil salinization(both the degree and type),shrub community structure(e.g.,shrub density and height),and biodiversity parameters(e.g.,Simpson diversity,Margalf abundance,Shannon-Wiener diversity,and Pielou evenness indices)were used to assess the effects of soil salinization on shrub community structure.The results showed that the primary degree of soil salinization in the study area was light salinization,with the area proportion of 69.8%.Whereas the main type of soil salinization was characterized as sulfate saline soil,also accounting for 69.8%of the total area.Notably,there was a significant reduction in the degree of soil salinization and a shift in the type of soil salinization from chloride saline soil to sulfate saline soil,with an increase in longitude.Regional mean annual precipitation(MAP),mean annual evapotranspiration(MAE),elevation,and slope significantly contributed to soil salinization and its geochemical differentiation.As soil salinization intensified,shrub community structure displayed increased diversity and evenness,as indicated by the increases in the Simpson diversity,Shannon-Wiener diversity,and Pielou evenness indices.Moreover,the succulent stems and leaves of Chenopodiaceae and Tamaricaceae exhibited clear advantages under these conditions.Furthermore,regional climate and topography,such as MAP,MAE,and elevation,had greater effects on the distribution of shrub plants than soil salinization.These results provide a reference for the origin and pattern of soil salinization in drylands and their effects on the community structure of halophyte shrub species.
