摘要
Carpobrotus edulis(L.)N.E.Br.is an edible halophyte native to South Africa,where it has been traditionally consumed as food,revealing its nutritional value,and used in the treatment of human diseases probably due to the accumulation of bioactive compounds such as polyphenols.These compounds have potential to display valuable biological activities that could bring several benefits to the human being,including the mitigation of toxicity induced by food contaminants.Therefore,this study investigated biological activities with health-promoting potential of a C.edulis ethanol extract(CEE),such as antioxidant,antigenotoxic,and anti-mutagenic.CEE was shown by LC-DAD-ESI/MSn to be composed of 16 polyphenols.The extract exhibited antioxidant properties by protecting Saccharomyces cerevisiae viability and improving the redox state of HepG2 cells under oxidative stress.Antigenotoxicity of CEE against H_(2)O_(2)-induced oxidative damage was detected in HepG2 and Caco-2 cells by comet assay and subsequently observed in the dominant deletion assay in S.cerevisiae.Antigenotoxicity and antimutagenicity of CEE towards the neoformed contaminant benzo[a]pyrene was found in HepG2 and Caco-2 cells by comet assay and in Salmonella typhimurium using the Ames test,respectively.The disclosed antigenotoxic and antimutagenic properties of C.edulis further advance its potential for application in food and nutraceutical industries for health benefits.
基金
financial support provided by National funds through FCT-Portuguese Foundation for Science and Technology(PD/BD/135329/2017
COVID/BD/152478/2022)
under the PhD AgriChains(PD/00122/2012)
from the European Social Funds and the Regional Operational Programme Norte 2020
supported by the research units CITAB(UIDB/04033/2020)and CBMA(UIDB/04050/2020)
L.Custodio acknowledges FCT for the UIDB/04326/2020 and PTDC/BAA-AGR/1391/2020 projects,and Scientific Employment Stimulus(CEECIND/00425/2017)
supported by national funds through FCT/MCTES(PIDDAC):CIMO,UIDB/00690/2020(DOI:10.54499/UIDB/00690/2020)and UIDP/00690/2020(DOI:10.54499/UIDP/00690/2020)
SusTEC,LA/P/0007/2020(DOI:10.54499/LA/P/0007/2020)
National funding by FCT,P.I.
