【目的】对比分析武夷黑猪与杜洛克猪热休克蛋白B1(heat shock protein beta-1,HSPB1)结构和功能,为研究动物机体抗应激机制提供参考。【方法】通过克隆武夷黑猪和杜洛克猪HSPB1基因并测序,同时运用生物信息学方法比对相似性,构建系统...【目的】对比分析武夷黑猪与杜洛克猪热休克蛋白B1(heat shock protein beta-1,HSPB1)结构和功能,为研究动物机体抗应激机制提供参考。【方法】通过克隆武夷黑猪和杜洛克猪HSPB1基因并测序,同时运用生物信息学方法比对相似性,构建系统进化树,对比分析两者HSPB1蛋白的特性、结构和功能的差异,以及在两个猪种中各组织的表达水平。【结果】成功克隆武夷黑猪与杜洛克猪HSPB1基因,编码区均为624 bp,均共编码207个氨基酸,两者HSPB1核苷酸和氨基酸相似性分别为99.0%和98.6%,存在123^(G→T)、233^(T→C)核苷酸位点的变异,同时引起41^(E→D)、78^(L→P)氨基酸的差异。核苷酸序列进化树显示,两者均与蝙蝠亲缘关系最近;氨基酸序列进化树显示,两者均与羊亲缘关系最近。两者有相同的基因启动子与转录因子,在蛋白质的信号肽、跨膜区、保守基序、理化性质、亲/疏水性等特性上保持一致。进一步对比分析发现,该蛋白中存在1个仅可被糖原合酶激酶-3(glycogen synthase kinase-3,GSK-3)所识别的新的磷酸化位点,和4个可被化学因子结合作用的差异位点。武夷黑猪HSPB1蛋白在线粒体中占比比杜洛克猪少,氨基酸序列的二级结构、三级结构存在7个区域的差别。虽然两者都与磷酸化激酶互作密切,但武夷黑猪比杜洛克猪HSPB1蛋白多1个细胞骨架功能条目。HSPB1基因在两个猪种各组织中广泛表达,且品种间均无显著差异,其中都在背最长肌中表达量最高。【结论】成功扩增武夷黑猪与杜洛克猪HSPB1基因。相较于杜洛克猪,武夷黑猪HSPB1在细胞质中含量更高,且存在磷酸化位点和化学因子结合位点的差异。但两者在相同组织中的表达量无差异,都在背最长肌中表达量最高。结果为深入研究猪源HSPB1在抗应激反应中发挥的作用提供了理论基础。展开更多
OBJECTIVE To investigate the role of adventitial vasa vasorum in artery remodeling during the process of pulmonary artery hypertension(PAH),we checked the small heat shock protein 27/25(HSPB1)whether involved in patho...OBJECTIVE To investigate the role of adventitial vasa vasorum in artery remodeling during the process of pulmonary artery hypertension(PAH),we checked the small heat shock protein 27/25(HSPB1)whether involved in pathological basis of vascular remodeling.METHODS We explored the potential role of HSPB1 interacts with ectopic F1Fo-ATPase in the pulmonary vascular remodeling,investigate its effects on the endothelium cell dynamic,and further reveal its possible molecular mechanisms using hypoxic pulmonary hypertension rat model,transgenic mice and pulmonary adventitial vasa vasorum endothelial cell culture in vitro.RESULTS Our studies have shown that HSPB1 improves adventitial vasa vasorum angiogenesis and remodeling.We found that hypoxia induces-HSPB1 upregulation and HSPB1 interact with ectopic F1Fo-ATPase modulate adventitial vasa vasorum endothelial cell proliferation,migration and tube formation.And the inhibition of HSPB1can reverse the vascular inflammation and fibrosis amazingly.CONCLUSION Adventitial vasa vasorum plays an important role in vascular remodeling,and small heat shock protein 27/25 was involved in a variety of diseases during the development of PAH,which could an efficient therapeutic targets and prevention strategy for PAH clinical.展开更多
Cardiogenic shock(CS)is a common cause of mortality and treatment remains challenging despite advances in therapeutic options.CS is caused by severe impairment of myocardial performance that results in decreased cardi...Cardiogenic shock(CS)is a common cause of mortality and treatment remains challenging despite advances in therapeutic options.CS is caused by severe impairment of myocardial performance that results in decreased cardiac output,hypoperfusion of the end organ,and hypoxia.Clinically this presents as hypotension refractory to volume resuscitation with features of end-organ hypoperfusion requiring pharmacological or mechanical intervention.Acute myocardial infarction(AMI)accounts for 81%of patients with CS.Heat shock protein family B member 1(HSPB1)is a multifunctional protein induced by various stress factors and has a protective effect on cells.A large number of studies have demonstrated that HSPB1 plays an important role in regulating apoptosis.Recently,some studies have suggested that HSPB1 also participates in the autophagic process.HSPB1 are expressed in many cells of the cardiovascular system such as endothelial cells,cardiac muscle cells,monocytes,and platelets.They are up-regulated in response to inflammation,oxidative stress,or ischemia and protect cells against extracellular stress factors.Here,we explore the involvement of HSPB1 in apoptosis,autophagy,and CS.We speculate that HSPB1 may exert its anti-myocardial injury role via the regulation of apoptosis and autophagy;this may provide the basis for the development of new approaches for the prevention and treatment of CS.展开更多
文摘OBJECTIVE To investigate the role of adventitial vasa vasorum in artery remodeling during the process of pulmonary artery hypertension(PAH),we checked the small heat shock protein 27/25(HSPB1)whether involved in pathological basis of vascular remodeling.METHODS We explored the potential role of HSPB1 interacts with ectopic F1Fo-ATPase in the pulmonary vascular remodeling,investigate its effects on the endothelium cell dynamic,and further reveal its possible molecular mechanisms using hypoxic pulmonary hypertension rat model,transgenic mice and pulmonary adventitial vasa vasorum endothelial cell culture in vitro.RESULTS Our studies have shown that HSPB1 improves adventitial vasa vasorum angiogenesis and remodeling.We found that hypoxia induces-HSPB1 upregulation and HSPB1 interact with ectopic F1Fo-ATPase modulate adventitial vasa vasorum endothelial cell proliferation,migration and tube formation.And the inhibition of HSPB1can reverse the vascular inflammation and fibrosis amazingly.CONCLUSION Adventitial vasa vasorum plays an important role in vascular remodeling,and small heat shock protein 27/25 was involved in a variety of diseases during the development of PAH,which could an efficient therapeutic targets and prevention strategy for PAH clinical.
文摘Cardiogenic shock(CS)is a common cause of mortality and treatment remains challenging despite advances in therapeutic options.CS is caused by severe impairment of myocardial performance that results in decreased cardiac output,hypoperfusion of the end organ,and hypoxia.Clinically this presents as hypotension refractory to volume resuscitation with features of end-organ hypoperfusion requiring pharmacological or mechanical intervention.Acute myocardial infarction(AMI)accounts for 81%of patients with CS.Heat shock protein family B member 1(HSPB1)is a multifunctional protein induced by various stress factors and has a protective effect on cells.A large number of studies have demonstrated that HSPB1 plays an important role in regulating apoptosis.Recently,some studies have suggested that HSPB1 also participates in the autophagic process.HSPB1 are expressed in many cells of the cardiovascular system such as endothelial cells,cardiac muscle cells,monocytes,and platelets.They are up-regulated in response to inflammation,oxidative stress,or ischemia and protect cells against extracellular stress factors.Here,we explore the involvement of HSPB1 in apoptosis,autophagy,and CS.We speculate that HSPB1 may exert its anti-myocardial injury role via the regulation of apoptosis and autophagy;this may provide the basis for the development of new approaches for the prevention and treatment of CS.
